#METABOLOMICS WORKBENCH GH_1090_20240117_045159 DATATRACK_ID:4584 STUDY_ID:ST003050 ANALYSIS_ID:AN005001 VERSION 1 CREATED_ON 02-07-2024 #PROJECT PR:PROJECT_TITLE Plasma instead of serum avoids critical confounding of clinical metabolomics PR:PROJECT_TITLE studies by platelets PR:PROJECT_SUMMARY Metabolomics is an emerging and powerful molecular profiling method supporting PR:PROJECT_SUMMARY clinical investigations. Serum and plasma are commonly used without rational PR:PROJECT_SUMMARY prioritization. Serum is collected after blood coagulation, a complex PR:PROJECT_SUMMARY biochemical process involving active platelet metabolism. This may affect the PR:PROJECT_SUMMARY metabolome and increase the variance as platelet counts and function may vary PR:PROJECT_SUMMARY substantially in individuals. A multi-omics approach systematically PR:PROJECT_SUMMARY investigating the suitability of serum and plasma for clinical studies PR:PROJECT_SUMMARY demonstrated that metabolites correlated well (n=461, R2=0.991), whereas lipid PR:PROJECT_SUMMARY mediators (n=104, R2=0.906) and proteins (n=322, R2=0.860) differed PR:PROJECT_SUMMARY substantially between specimen. Independently, analysis of platelet releasates PR:PROJECT_SUMMARY identified most biomolecules significantly enriched in serum when compared to PR:PROJECT_SUMMARY plasma. A prospective, randomized, controlled parallel group metabolomics trial PR:PROJECT_SUMMARY with acetylsalicylic acid administered for 7 days demonstrated that the apparent PR:PROJECT_SUMMARY drug effects significantly differ depending on analyzed specimen. Only serum PR:PROJECT_SUMMARY analyses of healthy individuals suggested a significant downregulation of TXB2 PR:PROJECT_SUMMARY and 12-HETE, which were specifically formed during coagulation in vitro. Plasma PR:PROJECT_SUMMARY analyses reliably identified acetylsalicylic acid effects on metabolites and PR:PROJECT_SUMMARY lipids occurring in vivo such as a decrease in polyunsaturated fatty acids. The PR:PROJECT_SUMMARY present data suggests that plasma should be preferred above serum for clinical PR:PROJECT_SUMMARY metabolomics studies as the serum metabolome may be substantially confounded by PR:PROJECT_SUMMARY platelets. PR:INSTITUTE University of Vienna PR:DEPARTMENT Department of Analytical Chemistry PR:LABORATORY Gerner lab PR:LAST_NAME Hagn PR:FIRST_NAME Gerhard PR:ADDRESS Währingerstraße 38, 1090 Vienna, Austria PR:EMAIL gerhard.hagn@univie.ac.at PR:PHONE +43 1 4277 52375 PR:DOI http://dx.doi.org/10.21228/M88147 #STUDY ST:STUDY_TITLE Plasma instead of serum avoids critical confounding of clinical metabolomics ST:STUDY_TITLE studies by platelets (Part 1/3 - Plasma and serum eicosadomics) ST:STUDY_SUMMARY Metabolomics is an emerging and powerful molecular profiling method supporting ST:STUDY_SUMMARY clinical investigations. Serum and plasma are commonly used without rational ST:STUDY_SUMMARY prioritization. Serum is collected after blood coagulation, a complex ST:STUDY_SUMMARY biochemical process involving active platelet metabolism. This may affect the ST:STUDY_SUMMARY metabolome and increase the variance as platelet counts and function may vary ST:STUDY_SUMMARY substantially in individuals. A multi-omics approach systematically ST:STUDY_SUMMARY investigating the suitability of serum and plasma for clinical studies ST:STUDY_SUMMARY demonstrated that metabolites correlated well (n=461, R2=0.991), whereas lipid ST:STUDY_SUMMARY mediators (n=104, R2=0.906) and proteins (n=322, R2=0.860) differed ST:STUDY_SUMMARY substantially between specimen. Independently, analysis of platelet releasates ST:STUDY_SUMMARY identified most biomolecules significantly enriched in serum when compared to ST:STUDY_SUMMARY plasma. A prospective, randomized, controlled parallel group metabolomics trial ST:STUDY_SUMMARY with acetylsalicylic acid administered for 7 days demonstrated that the apparent ST:STUDY_SUMMARY drug effects significantly differ depending on analyzed specimen. Only serum ST:STUDY_SUMMARY analyses of healthy individuals suggested a significant downregulation of TXB2 ST:STUDY_SUMMARY and 12-HETE, which were specifically formed during coagulation in vitro. Plasma ST:STUDY_SUMMARY analyses reliably identified acetylsalicylic acid effects on metabolites and ST:STUDY_SUMMARY lipids occurring in vivo such as a decrease in polyunsaturated fatty acids. The ST:STUDY_SUMMARY present data suggests that plasma should be preferred above serum for clinical ST:STUDY_SUMMARY metabolomics studies as the serum metabolome may be substantially confounded by ST:STUDY_SUMMARY platelets. ST:INSTITUTE University of Vienna ST:DEPARTMENT Department of Analytical Chemistry ST:LABORATORY Gerner lab ST:LAST_NAME Hagn ST:FIRST_NAME Gerhard ST:ADDRESS Währingerstraße 38, 1090 Vienna, Austria ST:EMAIL gerhard.hagn@univie.ac.at ST:PHONE +43 1 4277 52375 ST:SUBMIT_DATE 2024-01-17 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male and female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - Donor_1_Plasma_after_Aspirin_1 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_1_Plasma_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_1_Plasma_after_Aspirin_2 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_1_Plasma_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_2_Plasma_after_Aspirin_1 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_2_Plasma_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_2_Plasma_after_Aspirin_2 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_2_Plasma_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_3_Plasma_after_Aspirin_1 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_3_Plasma_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_3_Plasma_after_Aspirin_2 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_3_Plasma_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_4_Plasma_after_Aspirin_1 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_4_Plasma_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_4_Plasma_after_Aspirin_2 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_4_Plasma_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_5_Plasma_after_Aspirin_1 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_5_Plasma_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_5_Plasma_after_Aspirin_2 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_5_Plasma_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_6_Plasma_after_Aspirin_1 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_6_Plasma_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_6_Plasma_after_Aspirin_2 Sample material:Plasma | Treatment:after_Aspirin RAW_FILE_NAME=Donor_6_Plasma_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_10_Plasma_after_Omega3_1 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_10_Plasma_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_10_Plasma_after_Omega3_2 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_10_Plasma_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_11_Plasma_after_Omega3_1 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_11_Plasma_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_11_Plasma_after_Omega3_2 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_11_Plasma_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_12_Plasma_after_Omega3_1 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_12_Plasma_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_12_Plasma_after_Omega3_2 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_12_Plasma_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_7_Plasma_after_Omega3_1 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_7_Plasma_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_7_Plasma_after_Omega3_2 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_7_Plasma_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_8_Plasma_after_Omega3_1 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_8_Plasma_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_8_Plasma_after_Omega3_2 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_8_Plasma_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_9_Plasma_after_Omega3_1 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_9_Plasma_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_9_Plasma_after_Omega3_2 Sample material:Plasma | Treatment:after_Omega3 RAW_FILE_NAME=Donor_9_Plasma_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_1_Plasma_before_Aspirin_1 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_1_Plasma_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_1_Plasma_before_Aspirin_2 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_1_Plasma_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_2_Plasma_before_Aspirin_1 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_2_Plasma_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_2_Plasma_before_Aspirin_2 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_2_Plasma_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_3_Plasma_before_Aspirin_1 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_3_Plasma_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_3_Plasma_before_Aspirin_2 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_3_Plasma_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_4_Plasma_before_Aspirin_1 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_4_Plasma_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_4_Plasma_before_Aspirin_2 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_4_Plasma_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_5_Plasma_before_Aspirin_1 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_5_Plasma_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_5_Plasma_before_Aspirin_2 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_5_Plasma_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_6_Plasma_before_Aspirin_1 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_6_Plasma_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_6_Plasma_before_Aspirin_2 Sample material:Plasma | Treatment:before_Aspirin RAW_FILE_NAME=Donor_6_Plasma_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_10_Plasma_before_Omega3_1 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_10_Plasma_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_10_Plasma_before_Omega3_2 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_10_Plasma_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_11_Plasma_before_Omega3_1 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_11_Plasma_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_11_Plasma_before_Omega3_2 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_11_Plasma_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_12_Plasma_before_Omega3_1 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_12_Plasma_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_12_Plasma_before_Omega3_2 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_12_Plasma_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_7_Plasma_before_Omega3_1 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_7_Plasma_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_7_Plasma_before_Omega3_2 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_7_Plasma_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_8_Plasma_before_Omega3_1 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_8_Plasma_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_8_Plasma_before_Omega3_2 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_8_Plasma_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_9_Plasma_before_Omega3_1 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_9_Plasma_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_9_Plasma_before_Omega3_2 Sample material:Plasma | Treatment:before_Omega3 RAW_FILE_NAME=Donor_9_Plasma_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_1_Plasma_1 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_1_Plasma_1 SUBJECT_SAMPLE_FACTORS - Donor_1_Plasma_2 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_1_Plasma_2 SUBJECT_SAMPLE_FACTORS - Donor_2_Plasma_1 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_2_Plasma_1 SUBJECT_SAMPLE_FACTORS - Donor_2_Plasma_2 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_2_Plasma_2 SUBJECT_SAMPLE_FACTORS - Donor_3_Plasma_1 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_3_Plasma_1 SUBJECT_SAMPLE_FACTORS - Donor_3_Plasma_2 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_3_Plasma_2 SUBJECT_SAMPLE_FACTORS - Donor_4_Plasma_1 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_4_Plasma_1 SUBJECT_SAMPLE_FACTORS - Donor_4_Plasma_2 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_4_Plasma_2 SUBJECT_SAMPLE_FACTORS - Donor_5_Plasma_1 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_5_Plasma_1 SUBJECT_SAMPLE_FACTORS - Donor_5_Plasma_2 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_5_Plasma_2 SUBJECT_SAMPLE_FACTORS - Donor_6_Plasma_1 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_6_Plasma_1 SUBJECT_SAMPLE_FACTORS - Donor_6_Plasma_2 Sample material:Plasma | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_6_Plasma_2 SUBJECT_SAMPLE_FACTORS - Donor_1_Serum_after_Aspirin_1 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_1_Serum_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_1_Serum_after_Aspirin_2 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_1_Serum_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_2_Serum_after_Aspirin_1 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_2_Serum_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_2_Serum_after_Aspirin_2 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_2_Serum_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_3_Serum_after_Aspirin_1 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_3_Serum_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_3_Serum_after_Aspirin_2 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_3_Serum_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_4_Serum_after_Aspirin_1 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_4_Serum_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_4_Serum_after_Aspirin_2 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_4_Serum_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_5_Serum_after_Aspirin_1 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_5_Serum_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_5_Serum_after_Aspirin_2 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_5_Serum_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_6_Serum_after_Aspirin_1 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_6_Serum_after_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_6_Serum_after_Aspirin_2 Sample material:Serum | Treatment:after_Aspirin RAW_FILE_NAME=Donor_6_Serum_after_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_10_Serum_after_Omega3_1 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_10_Serum_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_10_Serum_after_Omega3_2 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_10_Serum_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_11_Serum_after_Omega3_1 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_11_Serum_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_11_Serum_after_Omega3_2 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_11_Serum_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_12_Serum_after_Omega3_1 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_12_Serum_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_12_Serum_after_Omega3_2 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_12_Serum_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_7_Serum_after_Omega3_1 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_7_Serum_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_7_Serum_after_Omega3_2 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_7_Serum_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_8_Serum_after_Omega3_1 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_8_Serum_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_8_Serum_after_Omega3_2 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_8_Serum_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_9_Serum_after_Omega3_1 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_9_Serum_after_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_9_Serum_after_Omega3_2 Sample material:Serum | Treatment:after_Omega3 RAW_FILE_NAME=Donor_9_Serum_after_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_1_Serum_before_Aspirin_1 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_1_Serum_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_1_Serum_before_Aspirin_2 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_1_Serum_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_2_Serum_before_Aspirin_1 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_2_Serum_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_2_Serum_before_Aspirin_2 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_2_Serum_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_3_Serum_before_Aspirin_1 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_3_Serum_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_3_Serum_before_Aspirin_2 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_3_Serum_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_4_Serum_before_Aspirin_1 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_4_Serum_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_4_Serum_before_Aspirin_2 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_4_Serum_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_5_Serum_before_Aspirin_1 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_5_Serum_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_5_Serum_before_Aspirin_2 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_5_Serum_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_6_Serum_before_Aspirin_1 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_6_Serum_before_Aspirin_1 SUBJECT_SAMPLE_FACTORS - Donor_6_Serum_before_Aspirin_2 Sample material:Serum | Treatment:before_Aspirin RAW_FILE_NAME=Donor_6_Serum_before_Aspirin_2 SUBJECT_SAMPLE_FACTORS - Donor_10_Serum_before_Omega3_1 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_10_Serum_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_10_Serum_before_Omega3_2 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_10_Serum_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_11_Serum_before_Omega3_1 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_11_Serum_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_11_Serum_before_Omega3_2 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_11_Serum_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_12_Serum_before_Omega3_1 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_12_Serum_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_12_Serum_before_Omega3_2 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_12_Serum_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_7_Serum_before_Omega3_1 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_7_Serum_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_7_Serum_before_Omega3_2 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_7_Serum_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_8_Serum_before_Omega3_1 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_8_Serum_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_8_Serum_before_Omega3_2 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_8_Serum_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_9_Serum_before_Omega3_1 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_9_Serum_before_Omega3_1 SUBJECT_SAMPLE_FACTORS - Donor_9_Serum_before_Omega3_2 Sample material:Serum | Treatment:before_Omega3 RAW_FILE_NAME=Donor_9_Serum_before_Omega3_2 SUBJECT_SAMPLE_FACTORS - Donor_1_Serum_1 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_1_Serum_1 SUBJECT_SAMPLE_FACTORS - Donor_1_Serum_2 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_1_Serum_2 SUBJECT_SAMPLE_FACTORS - Donor_2_Serum_1 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_2_Serum_1 SUBJECT_SAMPLE_FACTORS - Donor_2_Serum_2 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_2_Serum_2 SUBJECT_SAMPLE_FACTORS - Donor_3_Serum_1 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_3_Serum_1 SUBJECT_SAMPLE_FACTORS - Donor_3_Serum_2 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_3_Serum_2 SUBJECT_SAMPLE_FACTORS - Donor_4_Serum_1 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_4_Serum_1 SUBJECT_SAMPLE_FACTORS - Donor_4_Serum_2 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_4_Serum_2 SUBJECT_SAMPLE_FACTORS - Donor_5_Serum_1 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_5_Serum_1 SUBJECT_SAMPLE_FACTORS - Donor_5_Serum_2 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_5_Serum_2 SUBJECT_SAMPLE_FACTORS - Donor_6_Serum_1 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_6_Serum_1 SUBJECT_SAMPLE_FACTORS - Donor_6_Serum_2 Sample material:Serum | Treatment:Plasma_Serum_comparison RAW_FILE_NAME=Donor_6_Serum_2 #COLLECTION CO:COLLECTION_SUMMARY SERUM/PLASMA: Blood samples were obtained at baseline and after 7 days intake of CO:COLLECTION_SUMMARY the study medication. On both study days two blood samples using 6 mL K3EDTA and CO:COLLECTION_SUMMARY serum collection tubes (both Vacuette, Greiner Bio-One GmbH, Kremsmünster, CO:COLLECTION_SUMMARY Austria) were obtained from each subject. EDTA-anticoagulated tubes were CO:COLLECTION_SUMMARY carefully inverted two times after blood draw and centrifuged immediately at CO:COLLECTION_SUMMARY room temperature at 2000 g for 10 min. In contrast, filled serum tubes were CO:COLLECTION_SUMMARY carefully inverted after blood draw and placed to sit upright for 15 to 30 CO:COLLECTION_SUMMARY minutes to allow clot formation. Then, tubes were centrifuged at room CO:COLLECTION_SUMMARY temperature at 2000 g for 10 min. Directly after centrifugation, 500 µL of CO:COLLECTION_SUMMARY plasma or serum, respectively, were transferred into pre-labelled Eppendorf CO:COLLECTION_SUMMARY safe-lock tubes and stored at -80°C until analysis. CO:SAMPLE_TYPE Blood (serum) and blood (plasma) #TREATMENT TR:TREATMENT_SUMMARY Subjects were randomized to receive either acetylsalicylic acid or Omega-3 TR:TREATMENT_SUMMARY capsules for 7 days. One study cohort was instructed to take 500 mg TR:TREATMENT_SUMMARY acetylsalicylic acid (Aspirin® 500 mg acetylsalicylic acid, Cellulose powder, TR:TREATMENT_SUMMARY maize starche) per day in the evening whereas the second study cohort was TR:TREATMENT_SUMMARY instructed to take two Omega-3 complex 870 mg capsules (Br. Böhm Omega-3 TR:TREATMENT_SUMMARY capsules, 1017 mg cold water fish oil equivalent to 870 mg Omega-3, consisting TR:TREATMENT_SUMMARY of 420 mg EPA, 330 mg DHA, 5μg Vitamin D equivalent to 200 IU, 6 mg Vitamin E, TR:TREATMENT_SUMMARY 30 mg Co-enzyme Q10) per day in the evening. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY SERUM/PLASMA: Frozen EDTA-anticoagulated plasma or serum was freshly thawed on SP:SAMPLEPREP_SUMMARY ice. For precipitation of proteins, plasma or serum (400 µL) was mixed with SP:SAMPLEPREP_SUMMARY cold EtOH (1.6 mL, abs. 99%, -20°C; AustroAlco) including an internal standard SP:SAMPLEPREP_SUMMARY mixture of 12S-HETE-d8, 15S-HETE-d8, 5-Oxo-ETE-d7, 11,12-DiHETrE-d11, PGE2-d4 SP:SAMPLEPREP_SUMMARY and 20-HETE-d6 (concentrations can be found below). The samples were stored SP:SAMPLEPREP_SUMMARY over-night at -20°C. After centrifugation (30 min, 4536 g, 4°C), the SP:SAMPLEPREP_SUMMARY supernatant was transferred into a new 15 mL FalconTM tube. EtOH was evaporated SP:SAMPLEPREP_SUMMARY via vacuum centrifugation at 37°C until the original sample volume (400 µL) SP:SAMPLEPREP_SUMMARY was restored. For solid phase extraction (SPE) samples were loaded onto SP:SAMPLEPREP_SUMMARY preconditioned StrataX SPE columns (30 mg mL-1; Phenomenex, Torrance, CA, USA) SP:SAMPLEPREP_SUMMARY using Pasteur pipettes. After sample loading, the SPE columns were washed with 5 SP:SAMPLEPREP_SUMMARY mL of MS grade water and eluted with ice-cold MeOH (500 µL; MeOH abs.; VWR SP:SAMPLEPREP_SUMMARY International, Vienna, Austria) containing 2% formic acid (FA; Sigma-Aldrich). SP:SAMPLEPREP_SUMMARY MeOH was evaporated using a gentle nitrogen stream at room temperature and the SP:SAMPLEPREP_SUMMARY dried samples were reconstituted in 150 µL reconstitution buffer (H2O:ACN:MeOH SP:SAMPLEPREP_SUMMARY + 0.2% FA–vol% 65:31.5:3.5). The samples were then transferred into an SP:SAMPLEPREP_SUMMARY autosampler held at stored at 4°C and subsequently measured via LC-MS/MS. SP:SAMPLEPREP_SUMMARY 12S-HETE-d8: 6.67 pg/µL 15S-HETE-d8: 6.67 pg/µL 5-Oxo-ETE-d7: 20 pg/µL SP:SAMPLEPREP_SUMMARY 11,12-DiHETrE-d11: 6.67 pg/µL PGE2-d4: 13.33 pg/µL 20-HETE-d6: 6.67 pg/µL #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY For LC-MS analyses, analytes were separated using a Thermo Scientific Vanquish CH:CHROMATOGRAPHY_SUMMARY (UHPLC) system equipped with a Kinetex C18-column (2.6 µm, XB-C18, 100 A° , LC CH:CHROMATOGRAPHY_SUMMARY Column 150 * 2.1 mm; Phenomenex) applying a gradient flow profile (mobile phase CH:CHROMATOGRAPHY_SUMMARY A: H2O + 0.2% FA, mobile phase B: ACN:MeOH (vol% 90:10) + 0.2% FA) starting at CH:CHROMATOGRAPHY_SUMMARY 35% B and increasing to 90% B (1–10 min), further increasing to 99% B within CH:CHROMATOGRAPHY_SUMMARY 0.5 min and held for 5 min. Solvent B was then decreased to the initial level of CH:CHROMATOGRAPHY_SUMMARY 35% within 0.5 min and the column was equilibrated for 4 min, resulting in a CH:CHROMATOGRAPHY_SUMMARY total run time of 20 min. The flow rate was kept at 200 µL min-1 and the column CH:CHROMATOGRAPHY_SUMMARY oven temperature at 40°C. The injection volume was 20 µL and all samples were CH:CHROMATOGRAPHY_SUMMARY analysed in technical duplicates. CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Phenomenex Kinetex XB-C18 (150 x 2.1mm, 2.6um) CH:COLUMN_TEMPERATURE 40 CH:FLOW_GRADIENT 0min with 35% B to 90% B (1–10 min), further increasing to 99% B within 0.5 CH:FLOW_GRADIENT min and held for 5 min. Solvent B was then decreased to the initial level of 35% CH:FLOW_GRADIENT within 0.5 min and the column was equilibrated for 4 min, resulting in a total CH:FLOW_GRADIENT run time of 20 min. CH:FLOW_RATE 200 µL/min CH:SOLVENT_A 100% water; 0.2% formic acid CH:SOLVENT_B 90% acetonitrile/10% methanol; 0.2% formic acid CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:MS_COMMENTS The Vanquish UHPLC system was coupled to a Q ExactiveTM HF Quadrupole-OrbitrapTM MS:MS_COMMENTS high-resolution mass spectrometer (Thermo Fisher Scientific, Austria), equipped MS:MS_COMMENTS with a HESI source for negative ionization to perform the mass spectrometric MS:MS_COMMENTS analysis. The MS scan range was 250-700 m/z with a resolution of 60,000 (at m/z MS:MS_COMMENTS 200) on the MS1 level. A Top 2 method was applied for fragmentation (HCD 24 MS:MS_COMMENTS normalized collision energy), preferable 33 m/z values specific for well-known MS:MS_COMMENTS eicosanoids and precursor molecules from an inclusion list. The resulting MS:MS_COMMENTS fragments were analysed on the MS2 level at a resolution of 15,000 (at m/z 200). MS:MS_COMMENTS Operating in negative ionization mode, a spray voltage of 3.5 kV and a capillary MS:MS_COMMENTS temperature of 253°C were applied. Sheath gas was set to 46 and the auxiliary MS:MS_COMMENTS gas to 10 (arbitrary units). MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS normalized AUC MS_METABOLITE_DATA_START Samples Donor_1_Plasma_after_Aspirin_1 Donor_1_Plasma_after_Aspirin_2 Donor_2_Plasma_after_Aspirin_1 Donor_2_Plasma_after_Aspirin_2 Donor_3_Plasma_after_Aspirin_1 Donor_3_Plasma_after_Aspirin_2 Donor_4_Plasma_after_Aspirin_1 Donor_4_Plasma_after_Aspirin_2 Donor_5_Plasma_after_Aspirin_1 Donor_5_Plasma_after_Aspirin_2 Donor_6_Plasma_after_Aspirin_1 Donor_6_Plasma_after_Aspirin_2 Donor_10_Plasma_after_Omega3_1 Donor_10_Plasma_after_Omega3_2 Donor_11_Plasma_after_Omega3_1 Donor_11_Plasma_after_Omega3_2 Donor_12_Plasma_after_Omega3_1 Donor_12_Plasma_after_Omega3_2 Donor_7_Plasma_after_Omega3_1 Donor_7_Plasma_after_Omega3_2 Donor_8_Plasma_after_Omega3_1 Donor_8_Plasma_after_Omega3_2 Donor_9_Plasma_after_Omega3_1 Donor_9_Plasma_after_Omega3_2 Donor_1_Plasma_before_Aspirin_1 Donor_1_Plasma_before_Aspirin_2 Donor_2_Plasma_before_Aspirin_1 Donor_2_Plasma_before_Aspirin_2 Donor_3_Plasma_before_Aspirin_1 Donor_3_Plasma_before_Aspirin_2 Donor_4_Plasma_before_Aspirin_1 Donor_4_Plasma_before_Aspirin_2 Donor_5_Plasma_before_Aspirin_1 Donor_5_Plasma_before_Aspirin_2 Donor_6_Plasma_before_Aspirin_1 Donor_6_Plasma_before_Aspirin_2 Donor_10_Plasma_before_Omega3_1 Donor_10_Plasma_before_Omega3_2 Donor_11_Plasma_before_Omega3_1 Donor_11_Plasma_before_Omega3_2 Donor_12_Plasma_before_Omega3_1 Donor_12_Plasma_before_Omega3_2 Donor_7_Plasma_before_Omega3_1 Donor_7_Plasma_before_Omega3_2 Donor_8_Plasma_before_Omega3_1 Donor_8_Plasma_before_Omega3_2 Donor_9_Plasma_before_Omega3_1 Donor_9_Plasma_before_Omega3_2 Donor_1_Serum_after_Aspirin_1 Donor_1_Serum_after_Aspirin_2 Donor_2_Serum_after_Aspirin_1 Donor_2_Serum_after_Aspirin_2 Donor_3_Serum_after_Aspirin_1 Donor_3_Serum_after_Aspirin_2 Donor_4_Serum_after_Aspirin_1 Donor_4_Serum_after_Aspirin_2 Donor_5_Serum_after_Aspirin_1 Donor_5_Serum_after_Aspirin_2 Donor_6_Serum_after_Aspirin_1 Donor_6_Serum_after_Aspirin_2 Donor_10_Serum_after_Omega3_1 Donor_10_Serum_after_Omega3_2 Donor_11_Serum_after_Omega3_1 Donor_11_Serum_after_Omega3_2 Donor_12_Serum_after_Omega3_1 Donor_12_Serum_after_Omega3_2 Donor_7_Serum_after_Omega3_1 Donor_7_Serum_after_Omega3_2 Donor_8_Serum_after_Omega3_1 Donor_8_Serum_after_Omega3_2 Donor_9_Serum_after_Omega3_1 Donor_9_Serum_after_Omega3_2 Donor_1_Serum_before_Aspirin_1 Donor_1_Serum_before_Aspirin_2 Donor_2_Serum_before_Aspirin_1 Donor_2_Serum_before_Aspirin_2 Donor_3_Serum_before_Aspirin_1 Donor_3_Serum_before_Aspirin_2 Donor_4_Serum_before_Aspirin_1 Donor_4_Serum_before_Aspirin_2 Donor_5_Serum_before_Aspirin_1 Donor_5_Serum_before_Aspirin_2 Donor_6_Serum_before_Aspirin_1 Donor_6_Serum_before_Aspirin_2 Donor_10_Serum_before_Omega3_1 Donor_10_Serum_before_Omega3_2 Donor_11_Serum_before_Omega3_1 Donor_11_Serum_before_Omega3_2 Donor_12_Serum_before_Omega3_1 Donor_12_Serum_before_Omega3_2 Donor_7_Serum_before_Omega3_1 Donor_7_Serum_before_Omega3_2 Donor_8_Serum_before_Omega3_1 Donor_8_Serum_before_Omega3_2 Donor_9_Serum_before_Omega3_1 Donor_9_Serum_before_Omega3_2 Factors Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Aspirin Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:after_Omega3 Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Aspirin Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Plasma | Treatment:before_Omega3 Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Aspirin Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:after_Omega3 Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Aspirin Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 Sample material:Serum | Treatment:before_Omega3 11_12-DiHETrE 16.6102 16.8856 17.2693 17.0842 16.8219 16.9172 17.4933 17.4578 16.6947 17.0107 16.3768 16.9933 17.0677 17.0884 17.1080 17.1125 16.4480 16.6956 17.3481 17.5928 17.6753 17.6135 16.9708 17.0499 17.2631 17.3753 16.8783 17.1453 17.3181 17.6223 17.4730 17.6648 16.8785 16.8959 16.9836 17.1611 17.1102 17.4142 17.3664 17.4971 16.6047 17.0300 17.2783 17.6032 17.4559 17.6125 17.4432 17.5845 16.7180 16.9229 16.6389 17.0029 17.1069 16.9293 17.2417 17.2932 16.7779 16.8093 16.7181 16.9560 17.0670 17.0994 17.0426 17.1949 16.4454 16.6049 17.2833 17.4735 17.5204 17.4734 17.1244 16.9106 17.0641 17.1401 16.8675 16.9877 17.2980 17.5056 17.5975 17.5149 16.8617 16.9347 17.4513 17.2766 17.3063 17.2243 17.3869 17.2676 16.5280 16.6274 17.3469 17.4236 17.5736 17.5489 17.6413 17.6370 12_13-DiHOME 20.7003 20.4475 22.2624 22.0086 20.3233 20.2129 20.2571 19.7929 20.2773 20.1490 20.2080 20.0918 21.5164 21.4028 19.6441 19.2640 20.9947 20.6271 20.0627 19.7387 20.7349 20.6342 22.3873 22.1961 20.6920 20.5014 23.1636 22.9555 20.3831 20.1474 20.2766 20.0580 20.2183 20.2238 20.7251 20.5496 22.6436 22.4957 22.3240 22.1674 20.3616 19.8791 20.7533 20.6520 19.6197 19.4413 21.0342 20.8566 20.8304 20.5822 22.0971 21.8808 20.2718 20.1233 20.1024 19.7378 19.9762 19.9599 19.7618 19.3595 21.6647 21.2761 19.5074 19.0978 20.5451 20.2352 19.9333 19.7388 20.6013 20.4614 22.3661 22.1563 20.5630 20.3469 23.1010 22.8751 20.0782 19.8027 20.0842 19.7364 20.0577 19.7427 20.5225 20.1791 22.5505 22.2854 22.2126 21.9908 20.0157 19.7526 20.3955 20.2083 19.5680 19.1258 21.0102 20.5764 12-HETE/8-HETE 16.4645 16.5338 16.6083 16.5007 17.3233 17.2363 16.5949 16.6001 16.4481 16.6461 16.3044 16.2945 16.6336 16.4485 15.6191 15.8991 15.9445 16.2241 17.2014 17.2542 16.9616 16.9253 16.4916 16.2290 17.0399 16.8573 16.3541 16.3511 17.2999 17.3011 17.1641 17.0835 16.3796 16.3274 17.8696 17.9727 16.3352 16.3448 15.7156 15.7442 16.2864 16.2093 17.3435 17.4530 17.3363 17.4870 16.6719 16.4771 17.2645 17.2633 19.2182 19.2725 20.6116 20.7114 18.4944 18.7306 18.3724 18.2027 18.7084 18.6289 22.7843 22.8060 21.8622 21.9072 22.7690 22.7764 23.0356 23.1632 21.9906 22.0467 21.3145 21.2648 18.6198 18.7594 22.0473 22.0487 21.9226 22.0142 21.4833 21.5945 19.9250 19.9726 18.8715 18.8412 22.9759 22.9371 22.8837 22.9392 23.3474 23.4214 22.1229 22.2170 22.2381 22.3132 21.9547 22.1574 13-HOTrE 19.0491 18.6506 18.8065 17.8674 17.2409 17.0531 20.4752 19.9594 20.7595 20.1806 17.2233 17.1337 18.0947 17.3309 16.8813 16.1201 17.0374 16.5155 17.1448 16.7474 17.4006 16.9315 20.1600 19.7438 18.6049 18.2775 19.4281 18.7051 18.7745 18.2162 18.2156 17.8894 17.2477 16.7809 18.0039 17.8793 21.1182 20.4640 17.9403 17.3042 17.1240 16.7193 17.9892 17.5171 17.1601 17.0632 18.0339 16.8963 20.5637 20.2153 18.8365 18.0458 16.9423 16.9690 16.9404 16.3851 17.1745 16.4274 17.0561 16.6312 18.0524 17.4188 16.4574 16.2853 17.3766 16.5031 17.0339 17.0829 18.4243 18.1598 20.0013 19.8934 18.8135 18.8242 21.1705 20.7086 17.2204 16.8830 17.0546 16.8841 16.9218 16.5229 17.9317 17.5027 17.9472 17.4331 19.7268 19.3663 16.8275 15.9611 17.3165 17.2089 17.4904 17.3619 18.5255 18.6421 13-OxoODE 16.7115 16.3924 16.1947 16.2009 16.8179 16.7328 16.6654 16.6424 16.5947 16.5810 16.6643 16.7333 16.9185 17.0276 16.2297 16.0332 16.1005 16.2892 17.4182 17.5763 17.9253 17.8017 16.8716 16.7464 17.4268 17.5333 17.7512 17.8860 17.0683 16.9405 17.2888 17.3869 16.8402 16.9211 17.1843 17.1302 16.8289 16.8831 17.2053 17.1095 15.1345 15.6222 18.1235 18.0798 16.8399 16.9411 17.0611 16.9018 17.3016 17.4282 17.6787 17.5064 17.3474 17.3624 18.5120 18.4350 17.8439 17.7959 18.0951 18.0928 17.8678 17.9747 17.1737 17.3932 16.8672 16.8858 18.4745 18.6494 18.3141 18.3435 17.7368 17.7425 18.4543 18.4391 17.9876 18.1192 17.4691 17.7215 17.6079 18.1580 17.5963 17.7684 18.9236 19.0132 18.4487 18.4355 17.9515 18.0883 17.0941 16.9459 18.9629 19.1064 18.4469 18.4799 18.2277 18.3040 14_15-DiHETrE 16.7073 17.0551 17.8980 18.2763 17.2579 17.7817 17.1086 17.4641 17.3638 17.8137 17.2163 17.7481 17.3434 17.4398 17.3269 17.5211 17.0586 16.8023 17.4665 17.5041 17.6518 17.7705 17.1190 17.4988 17.5652 17.7018 17.8912 18.1776 17.0336 17.4604 17.4426 17.6870 17.0541 17.5728 17.4716 17.5989 17.3959 17.7796 17.3607 17.5670 16.6038 17.1607 17.4721 17.7207 17.8065 17.9961 17.9279 18.0379 17.0033 17.0166 18.2579 18.4149 17.5399 17.0412 17.2691 17.1763 17.5424 17.4306 17.1524 17.1380 17.4724 17.2295 17.4776 17.4004 16.6559 17.0234 17.5742 17.5593 17.7357 17.5913 17.4745 17.4356 17.5903 17.6833 17.0850 15.3427 17.3207 17.1830 17.5106 17.4602 17.3138 17.1979 17.6705 17.3561 17.9639 17.8245 17.4478 17.3684 16.8579 16.8261 17.6302 17.6496 18.0504 17.8164 17.8129 17.7967 14(15)-EET 17.6586 17.7020 17.6264 17.7025 18.2633 18.3157 18.2712 18.2803 16.6620 16.7410 17.5934 17.7698 18.4252 18.4645 18.1203 18.0506 19.1484 19.0573 18.7359 18.7758 18.5211 18.6619 18.2950 18.3476 18.3726 18.4435 17.7629 17.9010 18.8687 18.8941 18.6426 18.7052 17.5577 17.6317 18.2709 18.4299 17.6017 17.8468 17.7198 17.8313 18.8225 18.8927 18.5567 18.6197 18.3517 18.3648 18.5473 18.5023 18.2838 18.4307 18.2302 18.3326 18.9915 19.1405 19.1695 19.2062 17.4680 17.5858 18.3916 18.4419 19.2304 19.3448 18.7143 18.8537 19.8714 19.9400 19.2868 19.5343 19.1113 19.1350 19.1310 19.3009 19.0209 19.0966 18.4564 18.5967 19.6164 19.7653 19.3932 19.4811 18.2099 18.2809 19.5099 19.4413 18.3217 18.3216 18.5384 18.5515 19.5851 19.6670 19.0586 19.1740 18.6754 18.7725 19.2374 19.3016 15deoxy-PGJ2 16.7804 16.2596 16.5113 15.6720 16.1008 18.3475 17.6040 16.7393 19.6991 18.9731 20.0562 19.8553 15.8475 16.5555 17.1492 16.4067 17.3713 17.2112 16.6382 16.2197 16.6535 16.1238 17.3661 16.7145 16.7952 16.3898 16.3154 15.6191 16.2839 15.0754 16.1041 15.4501 16.8849 16.4895 17.1936 16.8605 16.6495 16.1626 16.8824 16.6127 16.5634 17.1487 20.3361 19.8267 16.2416 15.9538 16.2202 15.7121 16.6773 16.1585 18.3226 17.2878 20.4255 19.7536 20.5810 19.9560 16.6218 15.8405 16.3972 15.4750 16.2029 15.2712 16.3740 14.4926 15.3253 14.6885 17.0072 16.5467 16.6336 15.9659 16.5525 16.2376 16.6646 15.6226 16.9171 16.3804 16.7186 16.0561 15.0955 15.0357 16.4799 15.9500 16.0881 15.7887 16.3306 15.6943 16.4947 16.0923 15.7822 15.2823 19.7909 19.4253 16.7189 16.2565 16.5097 15.4123 15-HETE 16.3767 16.6702 16.3791 16.6510 16.8595 16.9207 17.6174 17.5457 17.3633 17.4984 16.6062 16.4030 16.9123 16.8039 16.2632 16.0899 16.1727 15.8935 16.7106 16.9202 16.9653 16.8527 17.2314 17.0046 16.7838 16.8486 16.7291 16.7267 17.1244 17.0638 17.2849 17.3853 16.9723 16.6826 17.2426 17.3737 17.4170 17.3235 16.4157 16.4096 16.5376 16.7623 17.1415 17.2696 16.7854 16.9898 16.8822 16.5499 17.1856 17.1623 16.7130 16.6586 16.9396 17.0421 16.9136 17.0793 17.0061 17.0721 16.5649 16.6074 17.8644 17.9920 17.1590 17.2308 17.8697 17.9232 18.5402 18.5871 17.3145 17.0918 17.6800 17.7171 16.9070 17.0302 18.7854 18.9217 17.6229 17.7171 17.3979 17.5498 17.0232 17.0951 18.0103 17.9284 18.1152 18.2176 18.2313 18.4003 18.5928 18.6517 17.8435 17.9886 17.5091 17.6069 18.4179 18.3827 16-HETE 16.3652 16.8706 16.5739 16.1314 16.8064 16.9473 16.5718 16.5378 16.8962 17.0645 16.6065 16.9054 16.9868 16.6248 16.5488 16.5238 15.6262 14.9687 16.2028 16.4538 17.0279 17.1551 16.5374 16.5832 16.5588 16.4711 16.1506 16.1946 16.5054 16.7159 16.5950 16.7881 16.6309 16.5998 16.4230 16.6657 17.1837 16.8811 16.7330 16.5473 16.0318 15.9230 16.4312 16.6597 16.8164 17.2419 16.8852 16.4144 16.6812 16.3339 16.5814 16.5125 16.9526 17.1870 17.0261 16.8178 17.1719 17.1254 16.6766 16.6367 16.8683 16.8047 16.9823 16.7218 16.2133 16.0853 16.4653 16.3055 17.1693 17.0784 16.8235 16.8172 16.4008 16.6319 16.7411 16.4569 16.7968 16.9199 16.9330 16.9432 17.0498 16.7052 16.8691 16.7663 17.0899 17.0331 17.0435 17.1209 16.2614 16.1157 16.7480 16.8886 17.3431 17.1464 16.6571 16.5616 17(18)-DiHETE 15.9129 15.9242 16.6602 16.8806 15.4880 16.0294 16.9625 16.9710 15.4592 15.7041 15.8977 15.9943 17.4203 17.6802 18.0052 18.4631 17.7098 17.9354 16.3586 16.5257 16.9769 17.3829 17.5346 17.7747 15.5965 16.1444 16.1496 16.8017 15.8715 16.1765 16.2999 16.6205 15.4281 15.4578 15.6666 16.0049 16.0292 16.4147 16.1045 16.0949 17.0678 17.1855 15.9385 15.6993 16.1234 16.4826 16.1433 16.3685 15.6464 16.1137 16.4552 16.7615 15.5550 15.9936 16.6623 16.7204 15.6481 15.8674 15.8421 15.8242 17.4817 17.5775 18.0494 18.1431 17.5299 17.9114 17.4134 16.6759 17.0246 17.0882 17.6508 17.5788 15.8868 16.3050 16.5027 16.2529 16.0056 16.3224 15.9729 16.2597 15.7875 15.2399 16.0410 16.1627 16.1932 16.2065 15.7431 15.9931 16.9940 17.3249 15.9747 15.3511 15.8908 16.1084 16.4726 16.5199 18-HEPE 15.7204 14.5401 15.7279 15.1023 14.4120 16.1800 15.7741 16.5480 16.7686 16.3028 17.6613 17.3501 15.2815 15.8917 14.5583 14.9257 16.5732 16.2371 15.2695 14.0796 16.4552 16.5670 14.4901 15.2760 16.1952 15.5558 14.4154 15.5685 15.1242 17.1443 16.7277 15.4040 14.8348 15.8729 15.4831 17.1238 15.5753 17.5636 15.3452 13.6932 15.1775 16.0821 17.7044 17.1247 15.2518 14.7500 14.8814 15.2000 14.7647 15.2183 14.7420 15.6224 17.2757 16.7816 18.0279 17.1518 14.3712 13.7512 16.1363 17.1116 15.8781 15.7361 15.7955 15.9146 16.4543 16.1192 15.4972 15.5791 15.8181 16.1016 13.9747 14.0026 14.2307 15.3376 16.4856 15.1280 16.4037 15.4472 15.3896 15.1278 15.6862 15.8732 14.9852 15.2201 15.3286 15.6107 15.4511 16.4678 16.1652 15.9537 16.8997 16.7410 14.3010 14.1140 15.1788 15.3700 18-HETE 15.0012 15.0924 15.2459 15.1698 15.6874 15.9873 15.9567 15.6604 15.9320 15.7458 16.2351 14.9564 15.7675 16.0180 16.8093 16.9422 15.9770 16.0147 15.5531 15.6875 16.5465 16.3352 15.9795 15.8191 14.9488 15.5375 14.9854 14.6957 15.6317 15.8510 15.8697 15.9554 15.8895 15.7292 15.4106 15.4256 15.6825 15.6729 15.9715 15.8912 15.0221 15.4274 15.8219 15.7111 16.7126 16.3689 15.7230 16.0392 15.4534 15.6537 15.4227 14.8182 15.9548 15.6978 15.9656 15.8980 16.0794 15.7377 15.1065 15.1410 16.0623 15.7097 16.5086 16.9375 15.7109 15.3841 15.9520 15.8853 16.7891 16.3162 15.9933 15.8404 15.6325 15.3422 15.3069 15.4258 16.1522 16.1412 15.8975 15.7894 16.0212 15.9798 15.6495 15.4759 15.8660 15.6335 16.0589 16.2371 15.2638 14.7345 15.7695 15.4502 16.3843 16.4468 15.6479 15.7188 19-HETE 16.1139 16.1649 15.5248 15.2697 16.3725 16.3719 16.3254 15.9969 16.4099 16.1574 16.1571 15.8616 15.8585 15.9470 16.0735 15.6566 15.4044 15.1806 16.2886 16.1795 16.8960 16.6240 16.1256 15.9723 16.7553 16.7335 15.8732 15.3220 16.2907 16.3953 16.7158 16.5224 16.3899 16.2639 16.7029 16.3687 16.1530 16.1233 15.8239 15.6764 15.5002 15.1860 16.4809 16.1387 16.5906 16.6489 16.6046 16.4242 16.7294 16.0523 15.5353 15.5163 16.5438 16.3529 16.5693 16.2943 16.6257 16.4019 16.1044 16.1123 16.2248 15.9055 16.5621 16.1785 15.7344 15.5068 16.5895 16.5043 16.8932 16.7799 16.3348 16.0181 17.2836 17.1253 15.9408 15.9029 16.8204 16.6107 16.9881 16.9250 16.4780 16.2104 17.0458 16.9086 16.4069 16.4313 16.3703 15.9065 15.4404 15.5914 16.8320 16.8614 16.8270 17.0150 16.8096 16.7971 20-HETE 16.1432 16.2880 16.0143 16.0391 16.9147 16.8509 16.3208 16.3831 16.5563 16.2968 16.7191 16.8322 16.5440 16.0760 16.5094 16.3804 16.6687 16.6237 17.0852 17.2034 16.8134 17.0054 17.3985 17.2005 17.5278 17.5234 16.4803 16.3587 17.3219 17.2210 16.9935 16.9566 16.8608 16.5699 17.6083 17.7587 16.6814 16.1945 16.2912 16.3368 16.3542 16.4957 17.3751 17.3506 16.9137 16.7715 17.8818 17.7655 16.7240 16.5288 16.4047 16.0015 17.3697 17.2231 16.9868 16.6373 16.9032 16.6637 17.3453 17.0838 16.6564 16.1280 16.8173 16.8525 17.0648 16.8599 17.5669 17.2487 17.1793 16.9507 17.8244 17.5828 17.6888 17.4887 16.7677 16.0840 17.4980 17.3571 17.2187 17.0252 17.3037 17.1223 17.9872 17.8876 16.7795 16.3538 16.8243 16.9137 17.1574 16.9710 17.7359 17.6348 17.2468 16.9691 18.3278 18.0659 22-HDoHE 17.2969 17.1544 16.3244 16.3532 17.8635 17.8497 17.8021 17.6540 16.4129 16.3622 17.3167 17.4806 18.4651 18.3421 18.6552 18.6240 19.2152 19.1968 18.7398 18.7921 18.2132 18.2155 18.6455 18.5633 18.3942 18.4132 16.7650 16.7320 18.1878 18.1292 18.3722 18.3943 16.6855 16.6674 18.2593 18.3169 17.0337 16.8978 17.2576 17.3089 18.7643 18.7468 18.0233 18.0362 17.9360 17.9661 18.4194 18.3233 17.9228 17.6922 16.7987 16.3220 18.3532 18.1591 18.2485 18.1624 16.9554 16.7001 17.7852 17.6781 18.7886 18.6695 19.1340 19.1328 19.5737 19.5887 19.3295 19.2178 18.6498 18.5211 19.0793 18.9752 18.8389 18.6971 17.2421 17.0622 18.6442 18.4879 18.7731 18.7329 17.2619 17.1085 18.8302 18.9145 17.4699 17.3349 17.9378 17.7764 19.2355 19.1993 18.5882 18.4573 18.5681 18.3618 18.9901 18.8760 5(6)-EET 18.1634 18.0990 17.6201 17.6633 18.7602 18.9237 18.5490 18.4444 18.2677 18.1393 18.1519 18.1703 17.7146 17.7973 17.8381 17.7272 18.2096 18.0610 18.9191 18.8290 19.0519 19.2145 18.1534 18.2504 18.7450 18.7411 17.4144 17.5606 19.2224 19.3356 19.0433 19.1395 18.2371 18.2596 18.6192 18.6086 17.8096 17.9694 17.8410 17.9886 18.3901 18.3573 18.8819 18.9913 19.0852 19.1388 19.2767 19.2931 18.8299 19.0555 18.3421 18.4892 19.8030 19.9882 19.4997 19.5900 18.8975 19.0010 19.0257 19.0570 18.7243 18.7438 18.5516 18.6428 19.0775 19.1482 19.7091 19.8248 19.8609 20.0098 19.2063 19.2851 19.4976 19.6219 18.2093 18.2631 20.3052 20.4883 19.9464 20.0435 19.1290 19.2297 20.0440 20.0502 18.8092 18.7160 18.7738 18.8464 19.3409 19.3536 19.6076 19.7795 19.7608 19.7634 20.2613 20.3302 5-HETE 16.3380 16.5142 16.8880 17.0569 16.9291 16.9887 16.8325 16.9062 16.8480 16.6982 16.9124 16.9728 16.6523 16.5234 16.8786 16.9893 16.5870 16.7376 16.9110 16.9133 16.8024 16.9255 16.5076 16.5556 17.1828 17.1627 16.9428 16.8712 16.9687 17.1977 17.5830 17.5511 17.1633 17.1576 16.6151 16.7328 16.8743 16.7361 16.8907 16.8727 16.5830 16.5358 17.0128 17.1167 17.1232 16.9406 16.8779 16.9470 18.2589 18.5143 19.5155 19.5820 18.3660 18.3073 18.6687 18.9428 19.1278 19.1824 19.3274 19.2178 19.2792 18.9794 17.8521 17.8129 18.3236 18.2814 19.4137 20.1352 17.3659 17.4076 19.3187 18.9987 19.7902 19.9883 19.9213 19.7075 19.3442 19.1524 17.9780 18.2229 19.1171 19.1664 19.2636 19.2773 18.9225 18.9469 17.6418 17.6307 18.0689 18.0949 19.7117 19.8904 17.2361 17.7304 19.8988 20.2833 9_10-DiHOME 21.6187 21.3508 21.6035 21.3065 20.3123 20.1756 20.5664 20.3265 20.9328 20.7641 20.0025 19.8761 22.2604 21.9328 19.7487 19.5708 20.8047 20.6070 20.6725 20.3376 22.3535 22.2012 22.2107 21.8182 20.8856 20.7100 21.4797 21.1129 20.8958 20.6020 20.5024 20.3693 20.5917 20.5049 20.8444 20.7869 22.3478 22.2396 21.7495 21.5607 20.4621 19.9638 20.4376 20.3659 20.1711 19.9552 21.5127 21.2223 21.5204 21.2174 21.1290 20.9189 19.9082 19.5747 19.8778 19.4306 20.2151 20.0033 19.2095 19.1046 21.9753 21.6598 19.0699 19.1920 20.3736 20.0692 20.1187 19.6405 22.2599 21.9016 21.9991 21.5640 20.2642 19.8919 21.2032 21.0481 20.1670 19.9350 19.9731 19.6193 20.2095 19.9573 20.0671 19.9209 22.1191 21.9142 21.7269 21.2543 19.4818 19.3605 20.1914 19.9437 19.6143 19.4175 21.2538 20.9097 9- and 13-HODE_9(10)- and 12(13)-EPOME 21.9859 21.9488 22.9482 22.8258 21.3290 21.3788 23.5243 23.5391 24.1073 24.0698 20.7772 20.8245 22.6401 22.6628 20.2491 20.2611 21.1336 21.0958 21.3506 21.3430 22.3479 22.3372 23.3198 23.3110 22.0473 22.0272 22.9326 22.9258 22.2641 22.2701 21.9585 21.9864 21.5263 21.4954 21.5909 21.6638 24.4787 24.4282 22.6668 22.6734 20.6594 20.7124 22.3392 22.3855 21.6297 21.6105 21.9961 21.9834 23.4940 23.5064 22.9006 22.8611 21.2251 21.2736 20.8205 20.8839 21.5556 21.6157 20.3492 20.2876 22.5567 22.5501 20.1026 20.1882 21.3647 21.4032 21.4346 21.2827 22.9945 23.0299 22.9791 22.9808 22.0916 22.1215 24.3858 24.3867 21.2720 21.3375 21.0727 21.1314 21.1289 21.1507 21.5029 21.5630 23.0662 23.0554 23.4615 23.5033 20.4528 20.4354 21.8322 21.6707 21.7981 21.7517 22.7500 22.7246 9-HOTrE 19.5202 19.2728 21.2562 20.9861 18.4156 17.9933 21.2466 20.9546 21.5591 20.9522 18.4526 18.2640 20.2865 19.9036 18.0542 17.7442 18.4245 17.8854 18.0698 18.2156 19.1977 19.0281 22.1405 22.1074 20.5462 20.4787 21.8271 21.8899 20.2470 19.7088 18.9535 18.7503 17.7903 17.3152 18.5422 18.6521 22.1832 21.7658 20.0941 19.7680 17.7561 17.6261 19.0688 18.7904 18.1106 18.1877 18.8583 18.5551 21.2639 21.1479 21.1400 21.2116 18.1619 18.0971 18.8925 18.6456 18.3913 18.0772 18.2075 18.0411 20.0812 20.1788 18.2623 18.0657 18.4815 18.1205 17.9278 18.0541 19.8763 19.6686 22.0411 22.0943 20.7089 20.7098 22.5257 22.5483 19.2017 19.0539 17.9266 18.1146 17.5934 17.6135 18.7360 18.3883 19.8133 20.0909 21.1059 20.6815 17.6879 17.1187 18.1630 18.3781 18.6357 18.1938 19.6385 19.6129 9-HpODE 23.5268 23.3360 26.2605 26.1493 24.5687 24.5335 25.1535 25.1072 24.4786 24.3905 24.3931 24.4517 25.2876 25.3166 24.3834 24.4189 23.9372 23.9991 24.2839 24.2054 24.1626 24.0849 26.3755 26.2837 24.2461 24.2460 25.7375 25.7567 24.4498 24.4858 24.9942 25.0538 23.6253 23.5098 24.2268 24.3224 25.5723 25.4998 24.3721 24.4601 24.2572 24.3164 24.6511 24.5356 24.1060 24.0176 25.0431 25.0185 23.7553 23.7059 26.5001 26.5067 24.4513 24.5293 25.2217 25.2760 24.4732 24.5332 24.4444 24.3372 25.4718 25.4489 24.6089 24.7064 24.4665 24.5097 24.5208 24.5267 23.9196 23.9136 26.7204 26.7571 24.3985 24.3207 25.7315 25.8392 24.6528 24.6943 24.7491 24.7573 24.2277 24.2523 24.1232 24.1908 25.6013 25.5166 24.6060 24.6843 24.5064 24.5235 24.6229 24.6828 23.9227 23.8243 25.2556 25.2367 AA 23.6580 23.6451 23.2854 23.2653 24.0527 24.0648 24.1757 24.1354 23.1971 23.1507 24.1692 23.9018 23.1989 23.1091 23.7830 23.7765 23.2386 23.1927 23.6929 23.6548 24.0452 23.9687 22.5775 22.6588 24.3972 24.2999 23.2817 23.1149 24.2260 24.2388 24.4385 24.3670 23.9280 23.8103 23.7158 23.7298 23.7703 23.6460 23.4656 23.5615 23.7972 23.8108 23.7211 23.9885 24.5549 24.5288 23.8229 23.7815 23.5482 23.5401 23.8220 23.9052 24.0391 24.0187 23.9166 23.8753 22.7850 22.8704 24.0620 23.3905 23.8912 23.7823 24.0015 24.1052 23.9958 24.0373 24.4049 24.4169 24.2235 24.2089 23.1057 23.2132 24.1688 24.1643 24.0041 23.8881 24.3142 24.1209 24.2972 24.2889 23.4707 23.4621 24.6439 24.5535 23.9964 23.7822 24.1737 24.2219 24.3666 24.3599 24.0342 24.1279 23.9590 23.8942 23.8558 23.8799 AA / isoform I 20.7797 21.0874 20.8534 20.8567 21.5670 21.4292 21.8312 21.6691 20.1527 20.1279 21.4122 20.3040 20.8432 20.5348 21.0531 20.6573 20.4767 20.2864 21.3966 21.1307 21.8573 21.6930 19.4692 19.4782 21.6676 21.5288 21.1008 20.5593 21.9341 21.5891 21.8949 21.7982 21.1974 21.1409 20.8990 20.7678 21.6536 21.3375 21.1903 21.3318 20.8948 20.7945 21.5907 21.6815 22.1123 22.1632 21.4658 21.4052 21.2270 21.1999 21.5122 21.1818 21.9220 21.6292 21.8059 21.6940 19.8029 19.4482 21.6878 19.6352 21.6347 21.2795 21.4348 21.2078 21.2566 21.1034 22.4294 22.4821 22.5387 22.4135 20.0543 19.6780 22.1185 21.9962 22.0142 21.4672 22.4108 21.9229 22.3464 21.9875 20.8526 20.7388 22.5340 22.0126 22.1280 21.3407 22.1635 21.8895 21.5242 21.1759 22.1408 22.1588 21.7912 21.6191 21.7159 21.6651 AA / isoform II 14.2838 15.4167 17.1250 15.8374 15.5860 14.6594 16.0577 16.0206 15.3229 15.2588 15.9512 14.9611 14.6496 15.0267 14.7142 13.9847 14.7001 14.3810 15.5320 15.7264 16.3174 16.4803 15.1847 15.4699 15.7916 16.4080 14.1623 15.1005 14.9471 14.9364 16.4776 16.9753 12.9461 15.3584 16.0468 16.0549 15.8624 15.7376 14.3409 14.6957 14.3232 15.0352 15.5448 15.5794 15.0711 15.4214 15.3266 14.7476 15.1005 14.8208 15.8403 15.4817 16.5461 16.1324 16.4743 16.0946 14.0747 13.8681 16.1369 14.2578 15.9485 14.9972 14.0760 14.1277 14.3171 14.2328 15.9733 16.0469 16.0706 15.9451 12.9572 14.3070 16.1456 15.7009 16.0159 15.2683 16.7968 16.4376 16.8088 16.5044 14.3633 14.1187 15.8379 15.5106 16.5857 15.7685 16.1132 15.9138 15.1740 14.2787 15.8533 15.4243 15.2957 14.7641 14.9298 15.6098 Adrenic Acid 16.8367 16.7009 16.3527 16.0503 17.9687 16.9785 18.1384 18.1823 16.2902 16.1098 18.1022 17.1373 16.5097 16.3466 15.8075 15.8802 15.6919 15.4225 15.6950 15.0144 16.3307 16.0559 15.6024 14.9434 15.8833 16.0056 16.4238 15.5203 16.9604 17.4602 18.8968 19.1824 15.9377 16.2625 15.6339 15.8188 18.0924 17.6753 16.0308 16.1990 16.0373 15.8514 15.9981 15.8705 17.4134 16.4137 14.8755 15.5974 16.2540 16.2011 16.4059 15.7765 18.2384 17.8683 17.7633 17.3616 15.3966 14.6428 17.6858 15.6439 16.8472 15.9501 15.1829 15.1442 15.1187 14.8202 16.5474 16.6852 17.6956 17.6315 13.8010 13.7413 17.7178 17.3365 16.6487 15.9438 18.4022 17.7863 18.3778 18.2051 15.7037 15.5650 17.8212 17.6217 17.8536 17.0585 17.0145 16.8401 15.6082 14.7191 16.9728 16.5803 16.6525 16.2902 16.2354 15.6535 a-Linolenic Acid 18.4659 18.4583 20.7901 20.8339 19.4437 19.4805 20.2020 20.2276 18.3744 18.3348 18.9319 18.7179 18.6571 18.7215 18.4739 18.6019 18.3927 18.4122 20.8862 20.9139 20.1698 20.1774 20.1229 20.1638 21.5106 21.4753 22.7599 22.7632 21.3646 21.4024 20.9100 20.9238 19.1314 19.1507 20.4083 20.4187 19.7803 19.8475 19.3950 19.5090 18.4785 18.6670 21.8683 22.0218 20.8159 20.8028 21.3076 21.2269 18.0920 18.3166 19.4888 19.5808 18.6056 18.5192 18.9344 18.8989 16.5432 16.5594 18.3242 17.7387 18.2511 18.1645 18.0771 18.2065 17.9476 17.8336 19.7700 19.6879 19.1232 19.0488 18.7679 18.6327 19.9579 19.7763 21.0653 21.0433 19.6349 19.7153 19.3114 19.4308 17.5652 17.5942 20.0345 19.9877 18.7556 18.6665 19.0585 19.1123 17.6582 17.6777 20.1611 20.1607 19.0239 19.0006 19.3323 19.4940 Cholic Acid 20.9922 20.9451 19.9924 19.9101 21.7775 21.7633 21.2922 21.2242 22.3873 22.4133 20.5440 20.5673 19.7403 19.6115 25.2721 25.2916 21.0411 21.0577 17.0031 16.9797 24.7847 24.7142 22.3754 22.4009 21.1788 21.1503 19.9847 20.0842 20.4056 20.4307 19.8057 19.8586 21.9817 21.9168 19.7815 19.7882 19.0263 18.9602 20.7709 20.8325 23.2938 23.2170 16.8020 16.7108 21.3486 21.3217 22.3371 22.3258 20.7435 20.7200 19.5848 19.5747 21.4243 21.4403 20.9352 21.0046 21.9765 22.0052 20.2431 20.1725 19.3895 19.3044 24.9005 24.9035 20.5709 20.5112 16.2233 16.5047 24.3989 24.3598 22.0644 22.0765 20.9371 20.9660 19.7471 19.7301 20.2001 20.1907 19.3240 19.3033 21.6903 21.6468 19.4873 19.5331 18.5125 18.6201 20.4389 20.4465 22.8894 22.8471 16.2401 16.3133 21.1824 21.2392 22.1172 22.1309 Deoxycholic Acid 23.3575 22.7864 24.4616 23.6684 23.8616 23.2917 24.4512 23.9428 23.8899 23.2195 25.1827 24.9501 24.2281 23.6987 24.7867 24.3739 24.9270 24.5903 24.1219 23.7359 23.8592 23.4487 23.6649 23.2047 23.4300 23.0016 24.4440 23.7983 23.9147 23.2336 23.8918 23.3938 23.7758 23.2529 24.6379 24.4084 24.8973 24.4060 24.0644 23.6983 25.4710 25.0654 23.7759 23.4050 23.4524 23.0111 23.6901 23.2450 21.0720 20.3492 23.3642 22.4734 22.0573 21.4570 23.4655 22.9169 22.0999 21.3188 24.6135 24.2622 23.5124 22.8717 23.9796 23.4892 23.5256 23.0845 22.9191 22.3741 22.3349 21.7734 21.1799 20.5819 21.3559 20.7491 23.6148 22.8512 22.5151 21.7976 22.2357 21.5897 21.7451 21.2823 23.6733 23.3758 24.2195 23.6374 22.6180 22.1354 24.9543 24.5195 22.8823 22.4742 21.3765 21.0005 21.8525 21.3354 DGLA 16.7203 16.7338 16.0715 15.9233 16.7962 16.8415 17.4870 17.3213 14.9836 15.0204 17.0007 16.6626 17.0251 17.0957 16.4820 16.4276 15.8223 15.9064 16.6069 16.3876 16.7140 16.9165 14.2499 14.1608 17.3268 17.2881 16.3640 16.0375 17.3517 17.3111 17.6722 17.5981 16.1207 16.0272 16.4379 16.3112 17.7968 17.8006 16.4583 16.4771 16.3087 16.1326 16.8738 17.1096 17.2088 17.1136 15.8906 16.0166 15.9061 15.9983 15.9202 15.6297 16.2694 16.2551 16.5363 16.2381 14.0215 13.8935 16.3121 15.3396 16.5941 16.4154 16.1985 16.1170 15.2772 15.3915 17.0453 16.9941 16.9664 16.9418 13.5862 13.8671 16.9787 16.9243 16.1154 15.9493 16.8499 16.7059 17.0800 16.9195 15.1720 14.9845 17.9226 17.7382 17.5813 16.8624 16.5393 16.4918 14.9871 14.9221 17.2134 17.1391 16.1553 16.0892 15.0404 15.2095 DGLA / isoform I 16.2132 16.4334 15.5091 15.2724 17.0868 17.2343 16.8679 16.7673 15.5050 15.7067 16.7935 16.6960 16.0676 15.9012 16.1145 15.9941 16.3504 16.3419 16.6426 16.6083 17.2510 17.3936 14.6255 14.7041 17.0342 17.0832 15.8303 15.8240 17.8933 18.0427 17.2800 17.2474 16.5553 16.5540 17.3680 17.3322 16.5046 16.3358 15.7354 15.8486 15.9421 15.7120 17.1887 17.2787 17.5222 17.6640 16.4300 16.2488 15.9214 16.0169 15.6482 15.4936 16.7880 16.9896 16.1698 16.2352 14.8152 14.8757 16.3745 16.0280 15.5817 15.3117 15.6196 15.5470 15.5093 15.2908 16.4398 16.2857 16.7734 16.8061 14.0668 14.2226 16.1330 16.4232 15.7110 15.5622 17.5365 17.7107 16.8282 16.7279 15.5167 15.5554 17.3032 17.4549 16.1607 16.0122 15.8185 15.8033 15.5070 15.4528 16.5468 16.7042 16.7047 16.8146 15.8207 15.9047 DHA 22.9845 23.0906 23.6478 23.7242 23.7350 23.8059 24.1240 24.1459 22.0860 22.1355 23.6611 23.5895 24.1683 24.1818 24.3055 24.3853 24.8216 24.7784 23.9563 23.9894 24.0941 24.1396 23.7533 23.7924 23.4820 23.6324 23.6122 23.6083 24.1386 24.2469 24.5229 24.5892 22.6765 22.6274 23.5628 23.5589 23.1964 23.1905 23.6391 23.6417 24.6166 24.5542 23.6665 23.7642 23.7603 23.8150 23.7869 23.8127 23.9687 24.0395 24.3743 24.5097 24.3874 24.5301 24.4463 24.5432 22.7129 22.7279 24.3633 23.9997 24.7461 24.7306 24.8098 24.8804 24.8531 24.8705 24.7471 24.8096 24.6926 24.7776 24.3221 24.3774 24.1425 24.3117 24.3410 24.4168 24.7511 24.8107 24.8056 24.8612 23.2236 23.2731 24.6333 24.6458 23.9600 23.9718 24.3765 24.4127 24.7404 24.7667 24.2038 24.3124 24.2309 24.2816 24.4630 24.4659 DHA / isoform I 20.8324 21.0023 14.7638 14.7836 21.9314 22.0684 22.4520 22.4998 19.1293 19.2264 21.6892 20.9212 14.2892 16.3550 14.8792 15.9516 21.2751 15.6767 22.4721 22.4053 22.2341 22.1227 14.2575 13.8789 22.8157 22.6476 13.8250 15.3314 22.2928 21.6225 23.2721 23.1885 20.9654 20.8942 22.3106 22.2033 21.7642 21.2363 13.8013 15.5962 21.7913 21.6244 22.1949 22.4508 22.7037 22.7030 21.8241 21.7502 15.5705 16.0958 16.7123 14.7652 21.0775 14.7752 20.3464 20.6152 14.0737 14.6676 15.7030 13.2162 15.8566 16.0203 15.5579 13.3556 14.9912 15.3321 21.7437 21.9659 15.8164 21.3308 13.1890 14.4775 22.0598 21.7806 14.9480 14.8180 16.2491 16.8034 22.3133 22.9112 16.1371 15.4017 22.9858 22.9980 14.6992 14.2537 15.8409 16.4417 14.4632 13.8826 21.4851 21.7185 20.8181 20.8701 15.8231 16.0136 DHA / isoform II 18.0806 18.2343 18.6585 18.4349 19.0805 18.9294 19.5733 19.6145 16.9392 16.7335 18.6548 17.5671 19.0578 18.7446 19.0665 18.8723 19.0305 18.9427 19.5123 19.3954 19.6415 19.6376 17.7685 17.7724 19.0816 19.0833 18.9631 18.2792 19.6635 19.4738 20.0283 19.9170 17.9793 17.8212 18.5949 18.4143 19.0110 18.6178 18.7236 18.7965 19.2241 19.1633 19.2507 19.4198 19.4605 19.5018 19.1959 19.2659 18.1870 18.1023 18.5643 18.3735 19.0576 18.8738 19.1710 19.1136 16.1305 15.8109 18.7872 16.4211 19.2812 18.7680 19.0211 18.7709 18.9864 18.8051 19.8911 19.8347 20.0899 19.9888 17.6096 17.3657 19.2862 19.2356 18.9540 18.4943 19.5789 19.2956 20.0179 19.9171 17.2816 17.0879 19.9967 19.7327 18.8556 18.0907 19.0197 18.7967 18.7189 18.0721 19.3907 19.3554 18.9308 18.7755 19.1396 19.0407 DPA 21.1719 21.1654 21.0468 20.9389 21.9060 21.7853 22.0744 21.9976 20.0749 19.9567 21.4075 20.6096 21.4607 21.2386 21.3473 21.2322 21.3206 21.1850 21.8439 21.7491 22.1649 22.0555 19.9659 19.9355 22.2185 22.1532 21.2322 20.7267 22.5670 22.5238 22.5719 22.5115 21.3040 21.1931 21.6226 21.5601 21.8465 21.5550 21.0600 21.1647 21.1076 21.0680 21.9410 22.1571 22.4217 22.4062 21.8958 21.8475 21.0811 21.0375 21.0691 20.8830 21.8401 21.7311 21.5491 21.4389 19.2512 18.9409 21.3606 19.6509 21.5849 21.1788 21.2925 21.1385 21.0291 20.9067 22.3048 22.2793 22.4330 22.3630 19.8428 19.7304 22.1858 22.2179 21.3541 20.9922 22.5303 22.2290 22.4876 22.3595 20.4618 20.3017 22.8840 22.7162 21.5886 21.0802 21.4876 21.3579 20.5124 20.1192 22.1075 22.0998 21.6731 21.5916 21.6313 21.5579 DPA / isoform I 19.3828 19.3676 19.0966 19.0863 20.3258 20.3771 20.1148 20.0283 18.0760 17.9805 19.8902 19.6527 19.4049 19.3881 19.4388 19.4518 19.4567 19.3643 19.7473 19.6924 20.2793 20.2000 18.6520 18.6589 20.0794 20.0528 19.2830 19.1478 20.6819 20.5978 20.5362 20.5432 18.8927 18.8542 19.8113 19.8049 19.7867 19.6681 19.1447 19.1799 19.1107 19.0355 20.0023 20.1054 20.4844 20.4308 19.6653 19.6141 19.2275 19.2908 19.0851 19.1843 20.4572 20.4833 19.9589 19.8009 17.6206 17.6374 19.9296 19.1759 19.4209 19.3043 19.2662 19.3334 18.8988 18.8638 20.0594 20.0220 20.5754 20.4853 18.5240 18.5877 19.9996 20.0990 19.2826 19.0959 20.8913 20.6585 20.7526 20.6438 18.5533 18.5433 21.0347 20.8848 19.7530 19.4920 19.5586 19.5779 18.7759 18.6634 19.9666 20.0380 19.8381 19.9513 19.6438 19.6108 EPA 20.7429 20.7424 20.6600 20.7138 20.9443 20.9771 21.0183 21.0721 19.8079 19.9302 20.8807 20.7858 22.2134 22.2616 22.7263 22.7591 23.2849 23.2332 21.7755 21.7894 21.7797 21.8019 21.9342 21.9821 20.9523 21.0118 20.3655 20.3562 21.1940 21.2603 21.3117 21.3535 20.3591 20.3164 21.0075 21.0426 20.3278 20.4123 20.4416 20.4996 22.3081 22.3029 20.7275 20.7398 21.2522 21.2764 20.7882 20.7390 21.6842 21.8019 21.7426 21.9049 21.8790 21.9543 21.7997 21.8401 20.0713 20.1555 21.5121 21.3290 23.1295 23.1736 23.4947 23.5695 23.8393 23.9295 23.0363 23.1632 22.5144 22.5757 22.8490 22.9508 21.7345 21.7788 21.3990 21.5857 22.1130 22.2427 21.9440 21.9908 20.8562 20.8945 22.3246 22.3603 21.0678 21.1366 21.4743 21.5429 23.0478 23.0482 21.7444 21.7580 21.7303 21.7002 21.6136 21.7216 g-Linolenic Acid 18.4340 18.5130 17.2155 16.4424 18.8371 18.8825 18.2299 18.2280 17.4173 17.5487 17.6649 17.6100 18.4049 18.3894 17.3312 17.2338 17.1006 16.9982 18.6075 18.5306 19.1073 19.1367 16.0459 16.3766 18.8952 19.0042 14.5074 14.3468 19.4021 19.3796 18.8177 19.0490 18.1089 17.8563 18.4601 18.4824 18.6555 18.4925 17.7444 17.6036 17.3517 16.9442 19.1159 18.9276 19.2774 19.3190 18.1238 18.1580 18.5554 18.5354 17.5641 17.8295 18.8834 18.9980 18.2658 18.1649 16.6786 16.6706 17.5508 17.3631 18.7842 18.7036 16.9135 16.9411 16.7162 16.9287 18.6979 18.7159 19.1070 19.1884 16.0025 16.5875 19.1296 19.3637 15.8773 15.4856 19.2591 19.2983 19.0136 19.0714 17.2303 17.5287 19.0526 19.1057 18.7941 18.4898 17.6722 17.7193 16.8521 17.0843 19.1159 19.2259 19.1012 18.7575 18.0009 17.9375 Glycocholic Acid 20.3795 20.4050 21.0141 20.9219 19.9979 19.9003 20.8824 20.7742 18.8722 18.8294 20.7226 20.6752 21.9212 21.8099 20.8913 20.7414 20.7751 20.7599 16.3711 16.3152 21.9780 22.0000 22.4184 22.3705 19.8999 19.9145 22.3330 22.1183 20.3262 20.2166 20.7945 20.7503 20.3897 20.2492 20.7318 20.5536 20.2640 20.1459 22.7301 22.6100 20.5031 20.4273 15.8877 15.9874 20.8275 20.8464 20.4904 20.4727 20.2260 20.2279 20.6355 20.6543 19.7689 19.6291 20.5693 20.5139 18.5167 18.4788 20.4091 20.3290 21.7301 21.6107 20.5660 20.5198 20.3603 20.2859 16.2975 16.2345 21.6598 21.6918 22.2193 22.2157 19.6684 19.8599 21.9978 21.9257 20.2537 20.0145 20.3776 20.2668 20.1095 20.0587 20.5068 20.4452 19.9972 19.9718 22.3698 22.2968 20.4417 20.2371 15.8095 15.8888 20.5832 20.5641 20.3361 20.3574 Glycodeoxycholic Acid 27.1198 26.6311 26.2721 25.3336 25.1476 24.1653 25.5591 24.7222 24.9946 24.1495 27.3762 27.0596 26.9411 26.3581 26.7352 26.0948 26.7384 26.1492 21.6699 20.8254 25.7087 25.0731 27.6010 27.1625 27.1783 26.7575 26.6900 26.0187 26.2839 25.4702 25.2426 24.4212 26.0179 25.3866 25.6261 25.1505 26.9445 26.3732 27.0843 26.7240 26.1850 25.4008 23.0053 22.1094 24.7707 23.9774 25.5449 24.7286 26.9933 26.5905 25.9942 24.9675 24.9421 23.9969 25.4013 24.4659 24.6754 23.8592 27.1256 26.8071 26.8476 26.2049 26.4753 25.9567 26.2776 25.6444 21.5986 20.3996 25.5585 24.8913 27.3939 27.0432 26.9703 26.7124 26.5663 25.8793 26.1052 25.3368 24.9292 24.0226 25.7789 25.2227 25.3712 24.8083 26.7740 26.2899 26.8911 26.5092 25.9187 25.1638 22.8285 22.0380 24.6212 24.1229 25.4656 24.7543 HHTrE / isoform I 16.1760 16.1548 16.1774 15.9593 15.9793 14.9356 15.9611 15.9859 16.3413 16.2991 15.9249 16.0865 14.8271 15.1667 15.3079 14.8274 17.0784 16.7780 15.3380 16.5888 15.1444 16.3606 13.0533 13.7221 14.3266 14.4690 14.1534 14.7000 14.3721 15.4328 16.1141 16.0938 15.9982 15.7400 15.9936 17.3166 16.1508 14.8094 15.3441 13.4210 16.7309 16.7430 15.1419 15.5745 16.0569 13.3529 15.0750 16.5230 16.6029 16.2743 15.3494 15.6861 15.9025 15.0167 16.0785 16.0515 16.3936 16.2977 15.8993 15.7634 16.9469 16.4627 15.3623 16.0303 17.5152 16.9478 17.0614 17.2337 15.6911 15.5018 16.0891 16.3539 15.4300 15.7342 16.3541 16.3457 15.9375 15.8663 16.2047 12.8295 16.2584 16.6634 16.4687 16.3945 16.5924 16.6323 16.0485 15.9632 18.0671 17.6965 16.2463 16.2750 15.9780 15.9353 17.1550 17.0505 HpODE / isoform I 20.9503 21.0036 22.8463 22.7445 20.5405 20.6220 21.2198 21.0441 20.3340 20.1417 19.9875 20.0683 21.7420 21.5474 20.1338 20.0277 21.2093 20.9931 20.2250 20.4346 20.7698 20.7608 24.2327 24.2200 22.5022 22.3734 24.4903 24.5472 21.9731 22.0650 20.7941 20.8979 20.2967 20.3141 20.9881 20.9426 21.9584 21.9255 22.6070 22.5788 20.2565 20.0889 20.7384 20.7358 20.1281 20.1645 21.1660 21.1693 20.8985 21.0757 22.4219 22.3993 20.5249 20.4563 20.9167 20.9065 20.1009 19.9227 19.6496 19.4974 21.4374 21.4458 20.0974 20.0193 20.6651 20.4763 20.0949 20.0328 20.5316 20.5160 24.1436 24.2856 22.2066 22.1682 24.1542 24.3605 21.7694 21.8456 20.5015 20.5407 20.1469 20.2378 20.8490 20.8818 21.7484 21.7746 22.4663 22.3520 20.0440 19.8636 20.4373 20.4902 19.7847 19.7044 21.0921 21.1565 HpODE / isoform II 19.4102 19.1376 19.6400 19.3914 18.9833 18.6242 19.5183 19.2396 19.9995 19.8825 19.5370 19.4059 19.2256 18.9906 19.2439 18.9838 19.6612 19.6690 19.1992 19.0998 19.0504 18.9011 19.6427 19.3833 18.9805 18.7374 19.2724 19.0961 18.9621 18.6919 19.0513 18.9509 19.0181 18.7935 19.3024 19.2281 19.0365 18.8884 19.7547 19.6382 19.0390 18.8261 19.9453 19.7677 18.8781 18.8289 18.8502 18.6753 19.3038 19.1494 19.6362 19.2026 19.3438 19.0867 19.3916 19.0091 18.7688 18.4908 18.6537 18.2559 18.9197 18.7082 17.9388 17.6535 18.3379 17.8915 18.8508 18.5035 18.8354 18.6389 18.9808 18.6471 19.0990 18.8400 19.2841 19.0401 18.4791 17.9077 19.0581 18.7784 18.7389 18.5517 18.3712 18.2749 18.8271 18.6368 19.7720 19.5689 18.2466 17.9070 19.7660 19.5683 18.7074 18.4661 18.4338 18.2505 Linoleic acid 23.0935 23.0602 23.4106 23.2756 23.8197 23.6119 24.0030 23.9076 22.3495 22.2691 23.2265 22.3245 23.3332 23.0760 22.3807 22.2465 22.6587 22.5164 24.2096 24.0690 24.5158 24.3463 21.4641 21.4168 24.4305 24.3852 24.0889 23.4381 24.1688 24.1069 24.3600 24.2913 23.4459 23.2732 23.6401 23.5358 24.7921 24.3468 23.4905 23.5438 22.5607 22.4448 24.5387 24.6615 24.6421 24.6319 23.9390 23.8276 22.2550 22.2289 22.5507 22.2854 23.0029 22.7828 22.7461 22.6347 20.8543 20.5691 22.3178 20.5518 22.6312 22.0802 21.6245 21.4893 21.4876 21.3729 23.7263 23.7277 24.0899 23.9775 20.6401 20.4558 23.7878 23.7899 23.4607 22.9093 23.7467 23.3220 23.7200 23.5651 21.8331 21.6231 24.0956 23.8770 23.7368 22.9768 22.9505 22.8037 21.1501 20.7121 24.0562 23.9247 23.2358 23.0841 23.1479 23.0370 LPC (0:0/18:2) 25.9535 25.9895 26.7234 26.8027 26.1418 26.2513 26.3156 26.3352 26.4870 26.5595 25.7349 25.7505 26.4150 26.3991 26.2213 26.3140 26.1498 26.2029 25.6071 25.6408 25.8783 25.8971 26.1492 26.1724 25.8431 25.9406 26.5031 26.6346 26.2342 26.3230 25.9617 26.0230 26.1038 26.0978 25.4798 25.5195 26.5637 26.6073 26.3679 26.4310 25.9121 25.9256 25.6240 25.6669 25.8022 25.8405 25.6662 25.7080 25.8883 25.9861 26.5337 26.7324 25.9715 26.1807 26.1110 26.2128 26.1331 26.2731 25.4836 25.4933 26.2483 26.2938 25.9447 26.0291 25.7378 25.7552 25.5087 25.6731 25.7792 25.8727 25.8011 25.9394 25.5927 25.8223 26.4809 26.6527 26.0765 26.2866 25.8531 25.9473 25.8374 25.9554 25.5393 25.6449 26.3895 26.5099 26.1015 26.1147 25.6792 25.6945 25.5263 25.6966 25.5460 25.7199 25.7661 25.8495 LPC (0:0/20:4) 24.3820 24.3838 24.8937 24.9162 24.5333 24.5968 24.8368 24.8658 25.0185 25.0670 24.2937 24.2805 24.1643 24.5371 25.0018 25.0365 24.0056 24.0067 23.7575 23.7509 23.9596 23.9759 24.6389 24.5985 24.4261 24.5266 24.6592 24.7614 24.6401 24.6593 24.4165 24.4642 24.6524 24.6597 24.1177 24.1053 24.6272 24.4699 25.0076 24.9844 24.4310 24.1541 23.7726 24.1193 24.2422 24.2407 24.0533 24.0874 24.1214 24.2558 24.5272 24.6726 24.4872 24.7009 24.3582 24.4454 24.7153 24.8229 24.0713 24.0560 24.1002 24.1687 24.4994 24.5695 23.6768 23.7071 23.8705 24.0290 24.0673 24.1530 24.4573 24.5314 24.3186 24.5707 24.4499 24.6188 24.5005 24.6816 24.3245 24.4053 24.5068 24.5548 24.2652 24.3767 24.1450 24.2519 24.4155 24.4350 23.9377 23.9785 23.7583 23.9415 24.1609 24.3704 24.2777 24.4481 LPC (0:0/20:5) 21.4471 20.8913 21.5531 20.8160 21.2962 20.7852 21.2663 20.7553 20.7514 20.1747 20.3856 20.1531 22.8057 22.3324 23.6185 23.2717 23.4720 23.2135 21.1657 20.8690 21.1826 20.8859 22.5984 22.1290 21.2257 20.8693 21.0088 20.3792 21.4782 20.9535 21.1726 20.6992 20.5997 20.1667 20.4968 20.3813 20.3114 19.7996 21.1887 20.8537 22.5834 22.1532 19.8907 19.5258 20.9727 20.5997 20.1063 19.7953 21.4535 20.8286 21.4341 20.6296 21.2062 20.7783 21.0906 20.6484 20.5567 19.9168 20.2267 19.8530 22.6949 22.1741 23.4515 23.1098 23.2127 22.8382 21.3721 20.8921 21.3241 20.7644 22.4338 21.8254 21.1216 20.6576 21.0256 20.3577 21.4013 20.7527 21.0211 20.4372 20.5447 20.1845 20.7019 20.4320 20.1999 19.6059 20.8676 20.5425 22.3876 21.9686 19.8594 19.5095 21.0346 20.7130 20.4264 19.9216 LPC (0:0/22:5) 19.3587 19.5177 19.5942 19.6562 19.7257 19.7631 19.4749 19.6335 19.3526 19.3633 19.2832 19.1838 19.9109 19.9450 20.2028 20.2586 20.1781 20.1931 19.5524 19.5776 19.1091 19.0456 19.7694 19.7820 19.5820 19.5976 19.3457 19.4083 19.7841 19.8812 19.6878 19.6907 19.4452 19.4432 19.0075 18.9739 19.4946 19.4944 19.7559 19.8282 19.6805 19.7392 19.2127 19.2126 19.2823 19.4383 19.1509 19.1593 19.4980 19.5029 19.5592 19.7798 19.7109 19.8038 19.2731 19.4841 18.8683 19.0645 18.9364 18.8480 19.8101 19.8651 19.9843 20.0434 19.6070 19.7632 19.6200 19.7258 19.1344 19.1027 19.5082 19.7673 19.2879 19.5992 19.4782 19.6652 19.7563 19.7518 19.6352 19.5597 19.1445 19.2602 19.1247 19.3136 19.3847 19.4383 19.5764 19.5493 19.3829 19.5831 19.0885 19.2588 19.2551 19.4760 19.4493 19.5116 LPC (0:0/22:6) 21.6674 21.6892 22.5218 22.6004 21.9708 22.0564 22.2834 22.2789 21.7885 21.8077 21.8137 21.8204 22.3866 22.4230 23.1703 23.1661 22.6942 22.7254 21.8866 21.9429 21.6278 21.7368 22.6158 22.6181 22.3201 22.4433 22.1966 22.2697 21.9611 22.1126 22.4474 22.5127 21.4950 21.5542 21.4473 21.5098 21.7289 21.6858 22.4545 22.4896 22.9060 22.8476 21.4358 21.4619 21.8449 21.9413 21.5461 21.5943 21.8632 21.9419 22.4479 22.6198 22.0268 22.1974 22.0827 22.1594 21.5178 21.5838 21.6543 21.5836 22.3481 22.4789 23.0445 23.0230 22.4470 22.4896 22.1301 22.2430 21.8883 22.0002 22.5222 22.5474 22.2861 22.5949 22.2637 22.3869 21.9037 22.0535 22.2589 22.3795 21.3488 21.5151 21.7619 21.9512 21.6595 21.6485 22.2271 22.2184 22.7236 22.7448 21.5222 21.6564 21.9208 22.1220 21.8215 22.0142 LPC (18:2/0:0) 27.9366 27.9871 28.5804 28.6820 28.1782 28.2444 28.2944 28.3359 28.2675 28.2496 27.9471 27.9202 28.2711 28.3501 28.3109 28.3538 28.4479 28.4493 27.7106 27.7575 27.9345 28.0609 27.9751 28.0925 27.9033 28.0025 28.3869 28.5251 27.9105 28.3041 28.1142 28.1798 28.0306 27.9306 27.7807 27.7841 28.3729 28.4464 28.3414 28.3791 28.2070 28.1920 27.7191 27.7954 27.8270 27.8670 27.8029 27.7238 27.6585 27.7122 28.3345 28.3115 27.6587 27.8898 27.6450 27.7871 27.7897 28.0169 27.4592 27.4090 27.7777 27.8683 27.7410 27.8317 27.5597 27.6098 27.4405 27.6003 27.5936 27.6974 27.6481 27.7634 27.4283 27.6619 28.2231 28.2279 27.7533 28.0552 27.6674 27.6861 27.4842 27.6066 27.4730 27.5705 27.8756 27.9754 27.8266 27.8228 27.6388 27.7159 27.3881 27.5740 27.3682 27.5598 27.5753 27.6268 LPC (20:4/0:0) 26.4305 26.4886 26.5676 26.6646 26.8740 27.0003 26.7571 26.7831 27.0634 27.1199 26.7713 26.7520 26.4715 26.4913 26.8708 26.9359 26.6897 26.7074 26.3151 26.3333 26.4718 26.5329 26.8614 26.8668 26.7912 26.8706 26.4670 26.6152 26.9379 27.0534 26.8495 26.9262 26.8934 26.8984 26.6757 26.6993 26.5152 26.5426 26.9091 26.9271 26.7509 26.7760 26.3505 26.4173 26.6498 26.6653 26.6689 26.6676 26.4598 26.5479 26.4082 26.5754 26.6355 26.8425 26.3993 26.4905 26.6051 26.7075 26.3750 26.3719 26.2422 26.3003 26.5570 26.6314 26.0925 26.0891 26.0714 26.1943 26.4751 26.4936 26.5745 26.6757 26.4437 26.6772 26.4463 26.6124 26.6476 26.8794 26.5122 26.5094 26.4378 26.5279 26.5940 26.5726 26.2530 26.3574 26.5726 26.5487 26.3394 26.4107 26.1273 26.2677 26.3506 26.5421 26.5310 26.5884 LPC (20:5/0:0) 24.8043 24.6805 24.7476 24.6022 24.6107 24.5735 24.5362 24.4902 24.2739 24.1914 23.9890 24.0010 25.8229 25.8053 26.6213 26.6075 26.7316 26.7493 24.6142 24.5516 24.7055 24.6959 25.8264 25.7190 24.6744 24.5801 24.3572 24.2708 24.9590 24.8575 24.4645 24.4383 24.0621 24.0809 24.0271 24.0808 23.5846 23.4695 24.6492 24.6336 25.8544 25.8466 23.4340 23.4874 24.3573 24.3606 23.7416 23.7371 24.6866 24.6194 24.5374 24.3947 24.3769 24.4402 24.1854 24.2012 23.8252 23.7996 23.6990 23.6376 25.6192 25.5582 26.2695 26.3586 26.1332 26.1375 24.5990 24.4985 24.7377 24.6391 25.6299 25.5247 24.3238 24.3902 24.1451 24.0553 24.6209 24.6012 24.0093 24.0411 23.6073 23.6716 23.8933 24.0434 23.3272 23.2347 24.1720 24.1692 25.6135 25.5497 23.4342 23.3531 24.2959 24.2760 23.7466 23.6790 LPC (22:5/0:0) 21.6497 21.6400 22.2620 22.3351 22.0725 22.2429 21.9274 21.9886 21.8225 21.9187 21.5983 21.5892 22.0825 22.1582 22.8400 22.8313 22.5761 22.5215 21.6953 21.7370 21.1555 21.2037 21.7280 21.7273 21.8636 21.9751 21.9180 21.9645 22.2009 22.2875 22.0363 22.0838 21.8535 21.8706 21.3993 21.4085 21.7901 21.8320 22.4971 22.4869 22.2347 22.2509 21.4777 21.5170 21.4420 21.3922 21.2226 21.2383 21.1270 21.2957 21.6877 21.8151 21.6640 21.8605 21.3883 21.4715 21.3047 21.5053 21.3652 21.2761 21.6767 21.7295 22.2529 22.2321 21.8606 21.7522 21.0886 21.2082 20.6435 20.7353 21.2091 21.3224 20.9878 21.2918 21.2641 21.4521 21.7079 21.7304 21.5033 21.6493 21.3006 21.2641 21.4933 21.4655 21.3081 21.4377 22.0043 21.9085 21.6878 21.7400 20.6525 20.7353 20.5864 20.8214 20.6865 20.8428 LPC (22:6/0:0) 24.5541 24.5852 25.0686 25.1350 24.8864 24.9562 25.0334 25.0530 24.4983 24.5302 24.7604 24.7142 25.1156 25.1413 25.7477 25.8459 25.7544 25.7868 24.8001 24.8167 24.6804 24.6937 25.3593 25.3846 25.0728 25.1512 24.8082 24.8999 24.8454 24.9217 25.3473 25.3800 24.4199 24.3999 24.5527 24.5157 24.4336 24.4410 25.2590 25.2584 25.8416 25.8288 24.4158 24.4536 24.6959 24.7321 24.7112 24.7020 24.5463 24.6588 24.8534 25.0157 24.5714 24.7924 24.5893 24.6882 23.9739 24.1009 24.4436 24.4516 24.8487 24.9145 25.3540 25.4115 25.1412 25.1883 24.6989 24.8529 24.6784 24.7821 25.1799 25.2804 24.8065 25.0638 24.6791 24.8424 24.5405 24.7059 24.7699 24.9833 23.9025 24.0309 24.5341 24.6236 24.0870 24.1784 24.8229 24.8255 25.4472 25.4659 24.1423 24.3356 24.5376 24.7143 24.6103 24.7615 LPE (0:0/16:0) 21.9361 21.9684 23.0651 23.1134 22.6348 22.6709 22.2441 22.1754 21.6497 21.7200 21.9058 21.8661 22.6753 22.6705 22.5693 22.5148 21.8600 21.8370 21.9874 22.0912 21.2811 21.2447 22.1595 22.1946 21.3855 21.4801 22.6243 22.6993 21.9391 22.0213 21.9388 21.9927 21.1386 21.1062 20.3561 20.3409 22.2024 22.1999 21.8575 21.9281 22.3191 22.3016 22.0663 22.1117 21.5539 21.5901 21.2124 21.1667 21.7337 21.8021 23.0315 23.1440 22.3255 22.4271 21.9185 22.0201 21.4646 21.5141 21.7704 21.7341 22.5921 22.7083 22.0586 22.0872 21.8928 21.8373 22.1092 22.3142 21.2344 21.3529 21.8983 21.9659 21.3146 21.5346 22.5416 22.6674 21.7778 21.9640 21.8054 21.9092 20.9975 21.0518 21.0800 21.1320 22.2823 22.3078 21.6861 21.6881 22.1352 22.2374 21.7385 21.9836 20.6626 20.9128 20.8938 21.1022 LPE (0:0/18:1) 23.2687 23.3060 24.5272 24.5920 22.2741 22.3548 22.3631 22.3710 23.0063 23.0956 21.4010 21.3704 22.9349 22.9493 22.4683 22.5179 22.0845 22.0963 21.2072 21.2196 21.1909 21.3066 22.0958 22.1074 21.9198 22.0077 23.9883 24.1223 22.2001 22.3072 21.3982 21.5198 21.4732 21.4815 20.1287 20.1993 23.3115 23.2743 22.5656 22.5439 21.8479 21.8475 21.3934 21.4959 21.3676 21.3623 21.2063 21.1576 23.0513 23.1490 24.2602 24.4351 21.9912 22.1079 22.0996 22.1457 22.8640 22.9803 21.1081 21.1871 22.7676 22.8143 22.0604 22.0895 21.6687 21.6622 20.9354 21.0233 21.0994 21.1835 21.7577 21.9105 21.7647 21.8781 23.6656 23.7413 22.0317 22.2313 21.3266 21.4010 21.2635 21.2512 20.7126 20.7716 23.2203 23.2251 22.3704 22.4253 21.6172 21.6261 20.9350 21.1521 20.4734 20.6196 20.8367 20.9077 LPE (0:0/18:2) 24.7334 24.6862 25.7774 25.6440 24.4490 24.4615 24.2818 24.2260 23.9765 23.9227 23.4573 23.4664 24.5240 24.4639 24.0220 24.0276 23.3961 23.3571 23.6826 23.6427 23.6176 23.6193 23.9887 23.9362 24.0136 23.9874 25.4313 25.3880 24.1502 24.0884 24.1182 24.1347 23.3594 23.3458 22.3267 22.3847 25.0732 24.9517 24.6169 24.6137 23.4876 23.4895 23.9803 23.9705 24.0642 24.0542 23.3308 23.2446 24.6886 24.6191 25.6923 25.5584 24.3680 24.4668 24.0993 24.0723 23.6608 23.6508 23.2837 23.2591 24.5339 24.5440 23.8458 23.9387 23.3513 23.3347 23.6953 23.6586 23.6230 23.5530 23.7640 23.6621 23.8635 23.8524 25.4061 25.3138 24.0915 24.1134 24.1014 24.1472 23.2361 23.2607 22.8190 22.9325 24.9637 24.9171 24.3803 24.3764 23.4537 23.4475 23.8726 23.8769 23.5955 23.5780 23.4230 23.3445 LPE (0:0/20:4) 23.7369 23.6923 24.1833 24.0219 24.2975 24.2813 24.1575 24.0767 24.0937 24.0283 24.2838 24.2694 23.2454 23.2021 23.7715 23.7199 23.8281 23.8007 23.4448 23.4107 22.9482 22.9778 23.7269 23.6592 23.7387 23.7263 23.8286 23.7281 24.0061 23.9364 24.1564 24.1286 23.4075 23.3886 22.9868 22.9774 23.8621 23.7059 23.9728 23.9284 23.4711 23.4571 23.6045 23.6001 23.8402 23.8203 23.4833 23.3619 23.2176 23.1522 23.6028 23.5085 23.7710 23.8143 23.5200 23.4953 23.2260 23.2223 23.1564 23.1198 23.0445 22.9862 23.1888 23.2642 22.2297 22.2637 23.0830 23.0553 22.6904 22.6657 23.1687 23.1348 23.3244 23.3127 23.4843 23.4571 23.4868 23.4476 23.8523 23.8286 23.0902 23.1237 23.0864 23.1839 23.4341 23.3868 23.3584 23.3486 22.8628 22.8838 23.3677 23.1305 23.0566 23.0261 23.1834 23.1067 LPE (0:0/20:5) 20.3117 20.2871 20.3618 20.6414 20.3074 20.6402 20.1703 20.3086 18.9565 18.6853 19.4384 19.7464 21.7183 21.6744 22.2557 22.1710 22.9385 22.8077 20.4826 20.3201 19.9527 19.9272 21.0963 20.9806 19.3838 19.3046 20.0888 19.6792 20.0420 20.2058 20.4046 20.4605 18.9976 18.8843 19.2978 18.9281 19.2887 19.4825 19.8966 20.1366 21.6303 21.8947 19.2881 19.3828 20.0505 19.7096 18.6193 18.5612 20.0205 19.8599 19.8640 19.6945 20.1032 20.0093 19.6818 19.8927 18.4148 18.2602 18.6699 18.6526 21.3070 21.1360 21.7894 21.7875 21.8832 21.4770 20.1491 19.6901 19.4139 19.2520 20.7465 20.5159 19.0286 18.9160 19.4384 19.3510 19.6006 19.4866 19.9649 19.8156 18.3154 18.6371 18.9676 19.1639 18.9012 18.7975 19.1939 19.2941 21.0352 20.9159 18.9434 18.9461 19.2599 19.3743 18.4925 18.5586 LPE (0:0/22:6) 22.4261 22.2312 22.8656 22.5798 22.7695 22.6151 22.9260 22.7492 22.3671 21.9467 22.5335 22.8146 22.9427 22.7790 23.3030 23.1916 23.2840 23.2060 22.8594 22.7644 22.0750 21.9449 22.9478 22.8247 22.8273 22.7699 22.2931 22.1163 22.3068 22.1211 23.2048 23.0971 21.7186 21.6690 22.2879 22.3243 22.2882 22.0408 22.4468 22.4115 23.4034 23.3284 22.8002 22.4145 22.5440 22.4091 22.2239 22.0091 22.1958 21.9031 22.4649 22.1634 22.5290 22.4463 22.7500 22.3706 21.5934 21.4069 22.0645 22.2437 22.8860 22.6737 22.8932 22.8719 22.9804 22.9408 23.0127 22.4890 21.8625 21.6584 22.5551 22.3647 22.5197 22.4254 22.0289 21.8462 22.3013 22.1704 23.2913 22.8837 21.8297 21.3991 22.0543 22.1768 21.9896 21.8086 22.0426 21.9689 23.3448 22.9277 22.4426 22.4571 22.0621 21.9616 22.1312 21.9566 LPE (16:0/0:0) 23.9658 24.0253 25.0631 25.1385 24.7210 24.8429 24.3415 24.2990 23.5108 23.6255 24.1967 24.1158 24.7811 24.8385 24.4506 24.5136 24.4004 24.3357 24.0054 24.0253 23.2898 23.3606 24.1846 24.2046 23.4029 23.4751 24.5904 24.7256 23.9593 24.0478 24.1954 24.2385 23.0373 23.0579 22.7080 22.7174 24.3610 24.3775 23.8309 23.8348 24.5864 24.5598 24.1168 24.1815 23.4872 23.5112 23.3713 23.3764 23.6667 23.7413 25.0718 25.1315 24.3617 24.4925 24.1061 24.1168 23.4222 23.5329 24.0435 23.9201 24.5175 24.5245 23.9415 24.0405 24.0773 24.0886 23.9359 24.0289 23.2976 23.3702 23.9729 24.0754 23.1813 23.3433 24.5249 24.6107 23.7892 23.9388 23.8587 23.8622 22.9031 22.9015 23.2133 23.2558 24.3567 24.3434 23.7425 23.7571 24.3709 24.4151 23.5862 23.7369 22.4842 22.6530 22.8877 22.9056 LPE (18:1/0:0) 25.2518 25.3414 26.4136 26.5001 24.4193 24.5200 24.2993 24.4105 25.0618 25.0943 23.6053 23.4506 24.9033 24.9277 24.5777 24.6602 24.2237 24.1891 23.2294 23.3023 23.2622 23.2836 24.1150 24.1610 23.8331 23.9049 25.8628 25.9422 24.3045 24.4134 23.5665 23.6383 23.4958 23.4640 22.3666 22.3431 25.1998 25.3243 24.7089 24.7465 24.0247 24.1300 23.4507 23.4668 23.2046 23.2511 23.2731 23.3328 25.1739 25.2232 26.5541 26.6360 24.2960 24.3714 24.3064 24.3784 25.0224 25.1103 23.4907 23.4046 25.0213 24.9491 24.3668 24.4392 24.1003 24.1879 23.0361 23.1396 23.2556 23.3687 24.0129 24.0950 23.7922 23.9417 25.7753 25.9074 24.3400 24.4537 23.4658 23.5974 23.2265 23.4107 22.8004 22.9015 25.4604 25.4355 24.8500 24.8885 24.1096 24.1423 22.9141 23.0926 22.5776 22.5735 22.9377 22.9597 LPE (18:2/0:0) 27.0599 27.1354 27.9522 28.0045 26.7036 26.9014 26.8193 26.8262 26.5766 26.6002 26.1462 26.1889 27.0459 27.1284 26.8365 26.8671 26.5885 26.5379 26.2597 26.3088 26.3054 26.3712 26.6270 26.6247 26.4396 26.4350 27.6433 27.7136 26.3729 26.4506 26.6565 26.6021 26.0641 26.1010 25.1919 25.2287 27.3487 27.3538 27.1369 27.1914 26.4539 26.3990 26.5702 26.5877 26.4598 26.5025 26.0275 26.0551 26.8725 27.0879 27.7207 27.8991 26.5767 26.7832 26.4813 26.5726 26.2286 26.3475 25.9493 25.9863 26.9339 27.0234 26.4357 26.5434 26.2314 26.2690 26.0677 26.1788 26.2122 26.3044 26.4108 26.5130 26.1875 26.3538 27.4630 27.6060 26.2918 26.4856 26.4578 26.5470 25.7198 25.8070 25.4545 25.5803 27.1167 27.2131 26.8426 26.9038 26.2523 26.3031 26.2472 26.4020 25.9238 26.0768 25.8682 25.9598 LPE (20:4/0:0) 26.0285 26.0596 26.3722 26.3969 26.2242 26.2626 26.1393 26.1551 26.3381 26.3925 25.8560 25.9063 25.6334 25.7614 26.2900 26.2676 25.6050 25.5911 25.8877 25.9586 25.6824 25.7193 26.2274 26.2205 26.1406 26.2393 26.1931 26.2823 25.9530 25.9824 26.4125 26.4980 26.0155 26.0467 25.4439 25.4852 26.0615 26.0482 26.2645 26.3264 25.8326 25.8553 26.1009 26.1206 26.0900 26.1446 25.9815 26.0113 25.8397 25.9569 26.1159 26.2437 25.8607 26.0992 25.7693 25.8631 25.9549 26.0060 25.5439 25.6112 25.5353 25.5882 25.9259 25.9815 25.1950 25.2219 25.7175 25.8424 25.5293 25.6117 25.9548 26.0339 25.9023 26.0872 26.0078 26.1141 25.3905 25.5761 26.1826 26.2571 25.6690 25.7910 25.5864 25.6866 25.8029 25.9043 25.9270 25.9811 25.5685 25.6716 25.7788 25.9586 25.6787 25.8132 25.8597 25.9442 LPE (20:5/0:0) 23.4697 22.9809 23.4628 22.7637 23.4240 23.0015 22.9573 22.3410 21.7971 20.9401 21.7123 21.5820 24.4136 23.9262 25.0380 24.6383 24.9120 24.5504 23.2697 22.9804 22.8530 22.6608 24.0860 23.8673 22.5941 22.2974 22.9634 22.2440 23.0556 22.5141 23.3956 22.8740 21.7403 21.1960 21.7745 21.3512 22.2200 21.7466 22.7988 22.3198 24.2037 23.6973 22.4754 22.0196 22.9501 22.6454 21.8579 21.6385 23.3373 23.2001 23.1810 22.6960 23.1838 22.9641 22.5457 22.0907 21.2735 20.7375 21.5138 20.9591 24.2412 23.8529 24.7602 24.5276 24.6130 24.0698 23.0516 22.8652 22.6159 22.5691 23.7676 23.7259 22.2967 22.2387 22.6456 22.4347 22.7579 22.3659 22.9497 22.4963 21.3647 21.0310 21.6706 21.3469 21.8968 21.5713 22.4298 21.8196 23.9283 23.4325 22.1002 22.1471 22.4869 22.5012 21.6286 21.5920 LPE (22:6/0:0) 24.8875 24.8494 25.1762 25.1558 25.1563 25.2169 25.0805 25.1232 24.8178 24.7192 24.7870 24.9109 25.2213 25.2903 25.6713 25.6952 25.4471 25.4870 25.3268 25.3749 24.7334 24.7404 25.4085 25.3723 25.2248 25.2883 24.7317 24.7645 24.7875 24.8451 25.5854 25.6347 24.3862 24.4333 24.6358 24.7212 24.6007 24.5569 24.9604 24.9875 25.7457 25.7593 25.2464 25.1261 25.0607 25.0566 24.8885 24.9298 24.6962 24.7746 24.9244 24.9979 24.8800 25.0551 24.8656 24.8149 24.2802 24.3162 24.5132 24.6713 25.0657 25.1370 25.3201 25.3671 25.2274 25.2122 25.2746 25.2112 24.5869 24.6303 25.1116 25.1232 24.9448 25.0957 24.4805 24.5512 24.4935 24.5682 25.4539 25.3928 24.1466 24.1379 24.6076 24.7285 24.3230 24.3440 24.6166 24.6820 25.6286 25.5414 24.9155 25.0030 24.6272 24.6918 24.8428 24.8188 LTB4/12-epi-LTB4 15.0737 14.4703 16.4578 15.9423 16.0947 15.6648 15.5549 14.2617 16.1551 15.2907 16.8473 16.5213 16.1012 15.7961 15.9251 15.2827 14.6793 15.3739 15.4549 16.8125 15.4911 16.0146 17.2189 14.4772 15.4868 15.2730 15.9865 15.1989 16.1156 15.3221 15.9268 15.7079 15.4234 14.1146 15.8966 15.9871 16.6882 15.8854 15.1502 16.5545 15.8223 14.6763 15.5917 15.4058 15.8114 15.4355 15.3279 14.7442 16.7998 16.3672 18.4648 17.4882 17.7762 17.1874 17.5345 16.6299 17.9585 17.0498 18.2882 17.5630 18.3984 17.2341 16.7473 15.7512 17.4431 16.4463 18.7094 17.9319 15.6652 14.5314 17.7760 16.6430 18.0742 17.4968 18.7963 17.7543 18.2009 17.2620 16.5179 15.5610 17.7122 16.9189 17.7978 17.0226 18.5425 17.5271 16.8918 15.9153 17.5506 16.5739 18.6727 18.2848 16.6602 16.1525 18.5653 18.2225 Oleic Acid 23.3484 23.3432 22.9041 22.7795 23.6265 23.4595 24.1314 24.1482 22.8614 22.7048 23.5414 22.7867 22.7252 22.4681 21.0415 20.9363 21.7754 21.8601 23.5239 23.3644 23.4822 23.2954 19.6124 19.6215 24.2237 24.1604 23.7711 23.0299 23.7381 23.6909 24.8986 24.8364 23.4303 23.2823 23.5278 23.4433 24.6091 24.1804 22.5329 22.6754 21.6068 21.5163 24.0142 24.1312 24.4084 24.3215 23.1336 22.9950 22.1839 21.9932 21.9078 21.3879 23.1141 22.5758 22.5392 21.7506 21.8235 21.3710 21.5166 19.7858 21.7995 20.4883 18.4365 18.5493 19.6339 19.3743 23.2856 23.1264 23.3333 23.1741 19.7065 19.1987 23.6068 23.6278 22.7768 21.6623 23.3568 22.6336 23.6781 23.3034 21.8260 21.5361 23.7881 23.5525 23.2563 22.3776 21.4873 21.2266 19.4863 19.1113 23.6026 23.3802 22.9086 22.9245 22.3336 22.0421 Palmitic Acid 17.9096 17.7506 18.2327 18.0721 19.0115 17.7636 19.4243 19.4207 17.5833 17.4626 19.1385 18.4361 18.1779 18.0459 17.7099 17.4437 18.0924 18.0514 15.0384 15.6111 15.3785 16.7121 15.8512 15.9225 16.4469 16.4693 18.2671 17.6089 17.3516 18.1499 19.9901 20.7189 16.9259 17.5523 16.7938 17.0116 19.7178 19.4644 18.2257 18.3526 17.6370 17.5278 15.3552 16.3476 17.9967 16.7461 15.5855 16.2496 16.2734 16.1155 17.1382 16.7264 17.8366 17.3544 17.7807 17.2647 16.2378 16.0127 17.0693 16.1549 16.9524 16.4056 15.6653 15.5923 15.6094 15.5268 16.9782 16.9969 17.2731 17.2702 15.2836 15.2442 17.7206 17.2083 17.8340 17.0719 17.8311 17.2320 17.9707 18.1336 15.5370 15.6111 17.0279 16.8360 17.9657 17.3059 17.3072 17.1687 15.8226 15.4380 17.6405 17.0606 16.8298 16.2912 15.7812 14.9848 PGE2 14.5064 16.1762 16.4134 14.4663 15.8579 15.6229 17.9601 17.0700 14.7355 15.3954 15.2733 14.8384 15.5361 16.0145 18.3596 14.3939 15.6678 14.5149 16.2816 15.8060 16.8553 16.5941 14.6746 14.2704 14.8220 14.7990 15.8083 15.3477 14.7857 14.9575 16.8842 16.0998 14.9166 14.8379 16.2439 15.7441 17.3283 16.3932 13.6791 16.1155 14.8143 15.0135 15.9428 14.6028 15.0038 16.1682 14.8003 14.3063 15.6186 15.9536 15.9265 13.7307 14.7294 15.0115 16.9498 16.1856 13.0992 14.6267 15.5063 14.5711 15.5945 15.5494 14.7245 14.5890 15.2797 15.2780 15.3515 15.5326 14.2298 14.4554 14.8499 14.8840 15.3678 15.0901 15.3406 15.1812 14.0498 14.0973 14.8021 14.8350 14.5839 15.3960 15.0387 13.7177 15.3330 15.4505 14.4383 14.3902 15.3920 15.5914 14.9660 14.9333 14.2804 14.6739 15.3876 15.4476 Sphingosine-1-phosphate 23.8381 23.4494 23.8449 23.4204 23.7786 23.2844 23.3831 22.8099 23.6599 23.1610 24.2540 24.0203 22.9297 22.4346 23.0976 22.7280 23.9174 23.5549 22.1808 21.7659 21.7787 21.4434 24.1147 23.6119 21.2256 20.8973 22.5884 21.9757 23.9303 23.4125 22.3930 22.0154 20.8179 20.4572 19.6712 19.1955 23.4301 22.9548 22.8506 22.4553 22.5347 22.1632 22.7692 22.3751 23.4546 23.0730 22.3235 21.9491 22.2831 21.6921 23.3205 22.4099 23.5857 22.9651 23.4961 22.9706 23.0395 22.6431 23.1370 22.6554 23.6501 23.0273 22.3292 22.1275 22.5935 22.2012 22.0984 21.6576 22.7488 22.3143 23.5390 22.8138 21.7570 21.2821 23.5185 22.6146 23.5265 22.7775 22.8033 22.1825 22.1431 21.7789 21.2809 21.1202 23.6996 23.0335 22.6353 22.2107 22.8167 22.2790 21.4300 21.2245 21.7228 21.3754 21.1628 20.7213 TXB2 15.7680 13.7577 15.9859 15.2984 15.3323 15.5257 15.1637 16.3157 13.9907 17.1923 17.3157 13.7374 15.6754 15.5280 14.0447 17.4312 16.5118 14.1991 15.2459 14.6311 16.1439 17.2321 14.2537 16.0735 15.0190 15.2476 15.1557 14.2541 15.2972 15.4502 17.2150 16.0325 16.2176 13.4201 17.8226 17.9340 15.4398 14.9773 14.0269 17.4573 15.3089 14.7511 13.8729 14.9639 15.2134 15.6896 14.6195 14.4369 14.5454 13.8497 16.1543 14.2808 17.4946 15.2351 15.8141 15.8093 15.5791 15.3208 16.0002 14.1392 20.2685 20.3046 19.2737 19.2940 20.4886 20.4969 20.9782 21.0265 19.0530 19.2004 20.0079 20.0505 16.5100 16.5706 20.6228 20.7769 19.2032 19.1098 19.0909 19.1893 17.2805 17.2813 16.3193 16.5065 20.1967 20.4231 19.1240 19.2045 21.1897 21.1717 19.9795 20.2295 19.3887 19.5574 20.7197 20.8627 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 11,12-DiHETrE 8.86 337.2384 12,13-DiHOME 8.18 313.2384 12-HETE/8-HETE 10.26 319.2279 13-HOTrE 9.12 293.2122 13-OxoODE 9.95 293.2122 14,15-DiHETrE 8.57 337.2384 14(15)-EET 10.86 319.2279 15deoxy-PGJ2 9.26 315.1966 15-HETE 9.96 319.2279 16-HETE 9.62 319.2279 17(18)-DiHETE 7.73 335.2228 18-HEPE 9.18 317.2122 18-HETE 9.51 319.2279 19-HETE 9.3 319.2279 20-HETE 9.38 319.2279 22-HDoHE 9.65 343.2279 5(6)-EET 11.27 319.2279 5-HETE 10.48 319.2279 9,10-DiHOME 8.34 313.2384 9- and 13-HODE_9(10)- and 12(13)-EPOME 9.79 295.2279 9-HOTrE 9 293.2122 9-HpODE 9.73 311.2228 AA 12.54 303.233 AA / isoform I 12.67 303.233 AA / isoform II 13.17 303.233 Adrenic Acid 13.16 331.2643 a-Linolenic Acid 12.03 277.2173 Cholic Acid 7.19 407.2797 Deoxycholic Acid 9.32 391.2854 DGLA 12.9 305.2486 DGLA / isoform I 13.06 305.2486 DHA 12.35 327.233 DHA / isoform I 12.43 327.233 DHA / isoform II 12.67 327.233 DPA 12.67 329.2486 DPA / isoform I 12.84 329.2486 EPA 11.88 301.2173 g-Linolenic Acid 12.13 277.2173 Glycocholic Acid 5.56 464.3018 Glycodeoxycholic Acid 7.98 448.3069 HHTrE / isoform I 8.76 279.1966 HpODE / isoform I 7.48 311.2228 HpODE / isoform II 8.47 311.2228 Linoleic acid 12.68 279.233 LPC (0:0/18:2) 10.93 564.3312 LPC (0:0/20:4) 10.67 588.3312 LPC (0:0/20:5) 9.4 586.3157 LPC (0:0/22:5) 11.33 614.3463 LPC (0:0/22:6) 10.45 612.3312 LPC (18:2/0:0) 11.63 564.3312 LPC (20:4/0:0) 11.17 588.3312 LPC (20:5/0:0) 9.75 586.3157 LPC (22:5/0:0) 12.17 614.3463 LPC (22:6/0:0) 10.87 612.3312 LPE (0:0/16:0) 10.55 452.2782 LPE (0:0/18:1) 11.09 478.2939 LPE (0:0/18:2) 9.75 476.2782 LPE (0:0/20:4) 9.73 500.2782 LPE (0:0/20:5) 8.77 498.2626 LPE (0:0/22:6) 9.63 524.2782 LPE (16:0/0:0) 11.04 452.2782 LPE (18:1/0:0) 11.6 478.2939 LPE (18:2/0:0) 10.09 476.2782 LPE (20:4/0:0) 10.02 500.2782 LPE (20:5/0:0) 9.04 498.2626 LPE (22:6/0:0) 9.9 524.2782 LTB4/12-epi-LTB4 7.85 335.2228 Oleic Acid 13.29 281.2486 Palmitic Acid 13.17 255.233 PGE2 5.42 351.2177 Sphingosine-1-phosphate 9.38 378.2415 TXB2 4.73 369.2277 METABOLITES_END #END