#METABOLOMICS WORKBENCH syfoods_20240218_013413 DATATRACK_ID:4655 STUDY_ID:ST003097 ANALYSIS_ID:AN005068 PROJECT_ID:PR001923 VERSION 1 CREATED_ON 07-06-2024 #PROJECT PR:PROJECT_TITLE Lactiplantibacillus plantarum intervention on gestational health of gestational PR:PROJECT_TITLE fecal microbiome transplant germ-free mice PR:PROJECT_SUMMARY The study aims to investigate whether administration of probiotic L. plantarum PR:PROJECT_SUMMARY could influence comprehensive gestational health by using germ-free mice, and PR:PROJECT_SUMMARY characterizing its influence on relationships between the gut microbiome PR:PROJECT_SUMMARY composition and metabolism. PR:INSTITUTE Beijing Sanyuan Foods Co. Ltd PR:LAST_NAME Lijun PR:FIRST_NAME Chen PR:ADDRESS 8th yingchang street, yinghai distric PR:EMAIL chenlijun@sanyuan.com.cn PR:PHONE 01056306594 PR:DOI http://dx.doi.org/10.21228/M85149 #STUDY ST:STUDY_TITLE Lactiplantibacillus plantarum intervention on cecum bile acids of gestational ST:STUDY_TITLE fecal microbiome transplant germ-free mice ST:STUDY_SUMMARY The study aims to investigate whether administration of probiotic L. plantarum ST:STUDY_SUMMARY could influence profiling of cecum bile acids of germ-free mice. L. plantarum ST:STUDY_SUMMARY during gestation of humanized GF mice showed that allolithocholic acid was ST:STUDY_SUMMARY significantly increased. Other bile acids showed no significant difference. ST:INSTITUTE Beijing Sanyuan Foods Co. Ltd ST:LAST_NAME Lijun ST:FIRST_NAME Chen ST:ADDRESS 8th yingchang street, yinghai district ST:EMAIL chenlijun@sanyuan.com.cn ST:PHONE 01056306594 ST:SUBMIT_DATE 2024-02-18 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENDER Female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 185MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801105.mzML SUBJECT_SAMPLE_FACTORS - 188MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801103.mzML SUBJECT_SAMPLE_FACTORS - 908MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801102.mzML SUBJECT_SAMPLE_FACTORS - 909MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801104.mzML SUBJECT_SAMPLE_FACTORS - 107MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801109.mzML SUBJECT_SAMPLE_FACTORS - 120MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801110.mzML SUBJECT_SAMPLE_FACTORS - 122MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801111.mzML SUBJECT_SAMPLE_FACTORS - 180MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801106.mzML SUBJECT_SAMPLE_FACTORS - 81MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801108.mzML SUBJECT_SAMPLE_FACTORS - 907MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801107.mzML #COLLECTION CO:COLLECTION_SUMMARY Cecum content sample was collected and stored at -80°C environment for bile CO:COLLECTION_SUMMARY acids analysis. CO:SAMPLE_TYPE Cecum CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Mice were randomly assigned into two experimental groups, named control and TR:TREATMENT_SUMMARY intervention groups, receiving standard chow feeding, and standard chow + L. TR:TREATMENT_SUMMARY plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS TR:TREATMENT_SUMMARY solution by oral gavage (2 days/week, p.o., three weeks). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Sample was extracted in 400 μL of methanol, and vortexing and shaking for 60 s, SP:SAMPLEPREP_SUMMARY add 100 mg of glass beads, and put into a high-throughput tissue grinder, SP:SAMPLEPREP_SUMMARY grinding at 55 Hz for 60 s. Repeat the above operation at least twice; SP:SAMPLEPREP_SUMMARY ultrasonic at room temperature for 30 min, centrifuged at 12,000 rpm at 4 ℃ SP:SAMPLEPREP_SUMMARY for 10 min, and 200 μL of supernatant mixed with 400 μL of water. Take 200 μL SP:SAMPLEPREP_SUMMARY of supernatant and add 400 μL of water, vortex for 30 s; take 300 μL of SP:SAMPLEPREP_SUMMARY supernatant and filter it through 0.22 μm membrane, vortex for 30 s. After SP:SAMPLEPREP_SUMMARY centrifugation, take 20 μL of supernatant and dilute it 10 times with 180 μL SP:SAMPLEPREP_SUMMARY of 30% methanol solution, and add it to the assay vial. SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:INSTRUMENT_NAME Shimmadzu LC-30 CH:COLUMN_NAME Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) CH:COLUMN_TEMPERATURE 40 CH:FLOW_GRADIENT 0-9 min, 30% B; 9-14 min, 30-36% B;14-18 min, 36-38% B;18-24 min, 38-50% B;24-32 CH:FLOW_GRADIENT min, 50-75% B;32-33 min, 75-90% B;33-35.5 min, 90-30% B CH:FLOW_RATE 5 μL/min CH:SOLVENT_A 0.01% formic acid in water CH:SOLVENT_B 100% acetonitrile CH:CHROMATOGRAPHY_TYPE LC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 6500+ MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE ESI MS:MS_COMMENTS Ion source temperature 500°C, ion source voltage -4500 V, collision gas 6 psi, MS:MS_COMMENTS air curtain gas 30 psi, nebulizer gas and auxiliary gas both 50 psi. Scanning MS:MS_COMMENTS was performed using multiple reaction monitoring (MRM). The raw data were MS:MS_COMMENTS firstly converted to mzXML format by MSConvert in the ProteoWizard software MS:MS_COMMENTS package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS MS:MS_COMMENTS (Domingo-Almenara et al., 2020) for feature detection, retention time correction MS:MS_COMMENTS and alignment. MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS μg/g MS_METABOLITE_DATA_START Samples 185MN 188MN 908MN 909MN 107MN 120MN 122MN 180MN 81MN 907MN Factors Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention ACA 23.3964 30.2381 20.4678 78.4980 40.6983 16.0303 11.1462 94.4578 43.9604 39.3786 alloLCA 0.9368 1.0333 0.7883 0.9202 1.3047 1.0242 1.0245 1.1133 1.1834 0.9181 alphaMCA 54.3260 57.3810 57.0760 139.2095 46.0848 17.5152 24.9012 122.6506 91.0099 64.0777 betaMCA 671.2274 569.0476 626.9006 1024.5059 568.5786 387.8788 495.6522 1096.3855 812.6733 710.6796 betaUDCA 0.8330 0.7690 0.8819 1.9091 0.3531 0.1085 0.1603 1.5904 1.2261 0.4777 CA 37.9074 12.4048 7.4854 144.9012 8.0499 1.9818 1.5249 237.8313 27.3267 3.9379 CDCA 3.3078 0.7143 2.8538 4.8617 0.6434 0.4091 0.2846 5.3253 0.6250 0.3775 DCA 185.6740 219.2857 200.0000 296.4427 196.0100 99.3939 113.8340 286.7470 266.1386 205.5146 GCA 0.3107 0.4265 GDCA 0.3810 0.4234 0.4150 0.4024 0.4444 0.4054 HDCA 3.3803 12.1667 3.8596 6.5929 6.2244 5.0000 7.4704 15.2048 10.1941 4.1010 isoLCA 3.7666 4.8333 4.3977 3.9605 4.5786 2.3333 3.9605 4.3373 3.8495 4.1476 LCA 34.5272 50.7143 47.4854 50.2767 42.1945 16.8182 32.9644 47.7108 40.6337 46.3689 LCA3S 3.0905 2.5476 3.2982 4.0791 4.4589 3.2424 2.5850 2.5542 2.7728 NorCA 4.1529 24.7619 6.2690 11.3123 25.5561 4.2727 2.4190 27.4699 12.5465 18.8272 NorDCA 1.0093 1.4190 0.8000 1.8925 2.4269 1.5121 0.5099 2.3325 1.0242 2.0691 S12ketoLCA 54.8089 46.9048 40.2339 62.1344 38.3042 27.5152 40.7905 43.8554 58.6931 66.4078 S67diketoLCA 0.4791 0.4482 0.2590 S712diketoLCA 1.8785 1.0762 1.2842 4.0553 1.3796 1.1000 0.8680 1.5880 1.5588 1.0882 S7ketoLCA 0.9272 0.3762 0.6246 3.0356 0.2050 0.1727 0.1383 1.7590 0.7747 0.2889 TalphaMCA 0.6519 0.2667 0.9099 2.4901 0.3112 0.1936 0.1629 2.3880 0.5370 0.2983 TbetaMCA 12.8451 5.5238 15.0877 41.2648 6.0748 3.2121 3.9605 101.9277 11.2396 7.3398 TCA 1.0769 0.8310 1.6842 2.3953 1.4274 1.3182 0.5858 3.8795 0.9244 0.8132 TCDCA 0.4443 0.3881 3.1345 0.6261 0.5147 0.4333 0.4269 0.5422 0.3208 0.4171 TDCA 1.1421 0.4643 1.5111 4.0791 0.6195 0.5000 0.3320 3.6867 1.2879 0.6641 THCA 0.3747 0.1730 THDCATUDCA 0.0927 0.0443 0.1836 0.3296 0.0658 0.8627 0.1359 0.0333 TLCA 0.3791 0.3977 0.4150 0.4313 UCA 1.8857 2.1429 1.4480 3.9368 3.2618 1.6424 1.0387 3.2771 3.2317 2.5631 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant ACA 0 21.6 407.304 alloLCA 94228 31.1 375.302 alphaMCA 0 15.6 407.3 betaMCA 0 16.6 407.302 betaUDCA 0 20.7 391.311 CA 0 22.1 407.303 CDCA 0 27.9 391.301 DCA 0 28.5 391.3 GCA 0 15.4 464.281 GDCA 0 24.1 448.279 HDCA 0 23.2 391.303 isoLCA C17658 31.4 375.288 LCA 9903 C03990 32.9 375.3 LCA3S 0 31.7 455.196 NorCA 0 16 393.211 NorDCA 0 25.7 377.301 S12ketoLCA 0 25.7 389.314 S67diketoLCA 0 25.3 403.3 S712diketoLCA 0 11.7 403.25 S7ketoLCA 0 30 389.3 TalphaMCA 0 5.67 514.311 TbetaMCA 0 6.02 514.287 TCA 0 14 514.332 TCDCA 0 21.7 498.357 TDCA 0 23.3 498.35 THCA 0 9.09 514.3 THDCATUDCA 0 12.9 498.411 TLCA 0 29.9 482.223 UCA 0 10.9 407.301 METABOLITES_END #END