{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003162","ANALYSIS_ID":"AN005189","VERSION":"1","CREATED_ON":"April 10, 2024, 6:57 pm"},

"PROJECT":{"PROJECT_TITLE":"Loss of dihydroceramide desaturase drives neurodegeneration by disrupting endoplasmic reticulum and lipid droplet homeostasis in glial cells","PROJECT_TYPE":"Untargeted Metabolomics and Lipidomics","PROJECT_SUMMARY":"Dihydroceramide desaturases convert dihydroceramides to ceramides, the precursors of all complex sphingolipids. Reduction of DEGS1 dihydroceramide desaturase function causes pediatric neurodegenerative disorder hypomyelinating leukodystrophy-18 (HLD-18). We discovered that infertile crescent (ifc), the Drosophila DEGS1 homolog, is expressed primarily in glial cells to promote CNS development by guarding against neurodegeneration. Loss of ifc causes massive dihydroceramide accumulation and severe morphological defects in cortex glia, including endoplasmic reticulum (ER) expansion, failure of neuronal ensheathment, and lipid droplet depletion. RNAi knockdown of the upstream ceramide synthase schlank in glia of ifc mutants rescues ER expansion, suggesting dihydroceramide accumulation in the ER drives this phenotype. RNAi knockdown of ifc in glia but not neurons drives neuronal cell death, suggesting that ifc function in glia promotes neuronal survival. Our work identifies glia as the primary site of disease progression in HLD-18 and may inform on juvenile forms of ALS, which also feature elevated dihydroceramide levels.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"Genetics, Medicine, Chemistry","LABORATORY":"Skeath and Patti Laboratories","LAST_NAME":"Cho","FIRST_NAME":"Kevin","ADDRESS":"1 Brookings Drive, Campus Box 1134, St. Louis, MO, 63130, USA","EMAIL":"kevin.cho@wustl.edu","PHONE":"314-935-8813"},

"STUDY":{"STUDY_TITLE":"Loss of dihydroceramide desaturase drives neurodegeneration by disrupting endoplasmic reticulum and lipid droplet homeostasis in glial cells","STUDY_TYPE":"Untargeted Metabolomics & Lipidomics","STUDY_SUMMARY":"Dihydroceramide desaturases convert dihydroceramides to ceramides, the precursors of all complex sphingolipids. Reduction of DEGS1 dihydroceramide desaturase function causes pediatric neurodegenerative disorder hypomyelinating leukodystrophy-18 (HLD-18). We discovered that infertile crescent (ifc), the Drosophila DEGS1 homolog, is expressed primarily in glial cells to promote CNS development by guarding against neurodegeneration. Loss of ifc causes massive dihydroceramide accumulation and severe morphological defects in cortex glia, including endoplasmic reticulum (ER) expansion, failure of neuronal ensheathment, and lipid droplet depletion. RNAi knockdown of the upstream ceramide synthase schlank in glia of ifc mutants rescues ER expansion, suggesting dihydroceramide accumulation in the ER drives this phenotype. RNAi knockdown of ifc in glia but not neurons drives neuronal cell death, suggesting that ifc function in glia promotes neuronal survival. Our work identifies glia as the primary site of disease progression in HLD-18 and may inform on juvenile forms of ALS, which also feature elevated dihydroceramide levels.","INSTITUTE":"Washington University in St. Louis","DEPARTMENT":"Genetics, Medicine, Chemistry","LABORATORY":"Skeath and Patti Laboratories","LAST_NAME":"Cho","FIRST_NAME":"Kevin","ADDRESS":"1 Brookings Drive, Campus Box 1134, St. Louis, MO, 63130, USA","EMAIL":"kevin.cho@wustl.edu","PHONE":"314-935-8813"},

"SUBJECT":{"SUBJECT_TYPE":"Insect","SUBJECT_SPECIES":"Drosophila melanogaster","TAXONOMY_ID":"7227"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_ifc_1",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_ifc_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_ifc_2",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_ifc_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_ifc_3",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_ifc_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_ifc_4",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_ifc_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_ifc_5",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_ifc_5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_WT_1",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_WT_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_WT_2",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_WT_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_WT_3",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_WT_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_WT_4",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_WT_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_RPLC_POS_WT_5",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"WL_RPLC_POS_WT_5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_ifc_1",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_ifc_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_ifc_2",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_ifc_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_ifc_3",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_ifc_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_ifc_4",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_ifc_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_ifc_5",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_ifc_5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_WT_1",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_WT_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_WT_2",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_WT_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_WT_3",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_WT_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_WT_4",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_WT_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_RPLC_POS_WT_5",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"RPLC","Polarity":"Positive","RAW_FILE_NAME(Raw file name)":"CNS_RPLC_POS_WT_5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_ifc_1",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_ifc_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_ifc_2",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_ifc_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_ifc_3",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_ifc_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_ifc_4",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_ifc_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_ifc_5",
"Factors":{"Genotype":"Knockout","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_ifc_5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_WT_1",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_WT_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_WT_2",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_WT_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_WT_3",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_WT_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_WT_4",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_WT_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"WL_HILIC_NEG_WT_5",
"Factors":{"Genotype":"Wild-type","Sample source":"Whole Larvae"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"WL_HILIC_NEG_WT_5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_ifc_1",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_ifc_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_ifc_2",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_ifc_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_ifc_3",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_ifc_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_ifc_4",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_ifc_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_ifc_5",
"Factors":{"Genotype":"Knockout","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_ifc_5.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_WT_1",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_WT_1.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_WT_2",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_WT_2.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_WT_3",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_WT_3.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_WT_4",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_WT_4.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"CNS_HILIC_NEG_WT_5",
"Factors":{"Genotype":"Wild-type","Sample source":"CNS"},
"Additional sample data":{"Chromatography":"HILIC","Polarity":"Negative","RAW_FILE_NAME(Raw file name)":"CNS_HILIC_NEG_WT_5.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Whole larvae and dissected CNS","SAMPLE_TYPE":"Larvae"},

"TREATMENT":{"TREATMENT_SUMMARY":"Untargeted lipidomics analysis was conducted on whole larva and dissected CNS of wild type and ifc-/- mutants at the late-third instar stage. Five replicates were prepared for each set of experiments. For whole larvae, at least 15 larvae of each genotype were used for each replicate. For the dissected CNS, at least 50 wild type and 60 ifc-/- CNS were used per replicate. Immediately following collection or dissection, larvae and the dissected CNS were flash frozen in liquid nitrogen and placed at -80°C."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Whole larvae and CNS samples were extracted utilizing a tissue homogenizer, employing a solvent mixture of acetonitrile, methanol, and water in a 2:2:1 ratio, using 40 µL per mg of wet weight. The samples were then stored at -20°C overnight. Following centrifugation at 14,000 x g for 10 minutes at 4°C, the supernatant was transferred into an LC/MS vial and subsequently stored at -80°C until LC/MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290 Infinity II","COLUMN_NAME":"Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um)","SOLVENT_A":"acetronitrile/water (60/40); 10mM ammonium formate; 2.5 uM medronic acid; 0.1% formic acid","SOLVENT_B":"2-propanol/acetonitrile (90/10); 10mM ammonium formate; 0.1% formic acid","FLOW_GRADIENT":"0-2min: 30% B, 2-17 min: 30%-75% B, 17-20 min: 75%-85% B, 20-23 min: 85%-100% B, 23-26 min: 100% B","FLOW_RATE":"250 uL/min","COLUMN_TEMPERATURE":"60"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 6545 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Data were collected with the following MS source settings: gas temp, 250°C; drying gas, 11 l/min; nebulizer, 35 psi; sheath gas temp, 300°C; sheath gas flow 12 l/min; capillary voltage, 3 kV; nozzle voltage, 500 V; isolation window, 1.3 Da. XCMS and Skyline software were used for data processing"},

"MS_METABOLITE_DATA":{
"Units":"peak area",

"Data":[{"Metabolite":"Cer 14:1;2O/18:0","WL_RPLC_POS_ifc_1":"2539","WL_RPLC_POS_ifc_2":"1702","WL_RPLC_POS_ifc_3":"1306","WL_RPLC_POS_ifc_4":"1134","WL_RPLC_POS_ifc_5":"1221","WL_RPLC_POS_WT_1":"163138","WL_RPLC_POS_WT_2":"156656","WL_RPLC_POS_WT_3":"195902","WL_RPLC_POS_WT_4":"150334","WL_RPLC_POS_WT_5":"145579"},{"Metabolite":"Cer 14:1;2O/20:0","WL_RPLC_POS_ifc_1":"7774","WL_RPLC_POS_ifc_2":"12261","WL_RPLC_POS_ifc_3":"12775","WL_RPLC_POS_ifc_4":"14526","WL_RPLC_POS_ifc_5":"6479","WL_RPLC_POS_WT_1":"477290","WL_RPLC_POS_WT_2":"439178","WL_RPLC_POS_WT_3":"608539","WL_RPLC_POS_WT_4":"478843","WL_RPLC_POS_WT_5":"494303"},{"Metabolite":"Cer 14:1;2O/24:0","WL_RPLC_POS_ifc_1":"901","WL_RPLC_POS_ifc_2":"2847","WL_RPLC_POS_ifc_3":"1161","WL_RPLC_POS_ifc_4":"540","WL_RPLC_POS_ifc_5":"2887","WL_RPLC_POS_WT_1":"11305","WL_RPLC_POS_WT_2":"12354","WL_RPLC_POS_WT_3":"21841","WL_RPLC_POS_WT_4":"13687","WL_RPLC_POS_WT_5":"11877"},{"Metabolite":"GlcCer d14:0/18:0","WL_RPLC_POS_ifc_1":"100365","WL_RPLC_POS_ifc_2":"134469","WL_RPLC_POS_ifc_3":"123915","WL_RPLC_POS_ifc_4":"129694","WL_RPLC_POS_ifc_5":"99063","WL_RPLC_POS_WT_1":"5511","WL_RPLC_POS_WT_2":"2509","WL_RPLC_POS_WT_3":"5156","WL_RPLC_POS_WT_4":"6668","WL_RPLC_POS_WT_5":"4267"},{"Metabolite":"GlcCer d14:0/20:0","WL_RPLC_POS_ifc_1":"103661","WL_RPLC_POS_ifc_2":"126464","WL_RPLC_POS_ifc_3":"106325","WL_RPLC_POS_ifc_4":"120548","WL_RPLC_POS_ifc_5":"90424","WL_RPLC_POS_WT_1":"2245","WL_RPLC_POS_WT_2":"2570","WL_RPLC_POS_WT_3":"3302","WL_RPLC_POS_WT_4":"988","WL_RPLC_POS_WT_5":"4520"},{"Metabolite":"GlcCer d14:0/22:0","WL_RPLC_POS_ifc_1":"30591","WL_RPLC_POS_ifc_2":"34474","WL_RPLC_POS_ifc_3":"26452","WL_RPLC_POS_ifc_4":"35016","WL_RPLC_POS_ifc_5":"24122","WL_RPLC_POS_WT_1":"1026","WL_RPLC_POS_WT_2":"1518","WL_RPLC_POS_WT_3":"1617","WL_RPLC_POS_WT_4":"2841","WL_RPLC_POS_WT_5":"1646"},{"Metabolite":"GlcCer d14:0/24:0","WL_RPLC_POS_ifc_1":"1793","WL_RPLC_POS_ifc_2":"1827","WL_RPLC_POS_ifc_3":"2593","WL_RPLC_POS_ifc_4":"1078","WL_RPLC_POS_ifc_5":"1240","WL_RPLC_POS_WT_1":"2640","WL_RPLC_POS_WT_2":"377","WL_RPLC_POS_WT_3":"2383","WL_RPLC_POS_WT_4":"541","WL_RPLC_POS_WT_5":"483"},{"Metabolite":"GlcCer d14:1/18:0","WL_RPLC_POS_ifc_1":"6180","WL_RPLC_POS_ifc_2":"1396","WL_RPLC_POS_ifc_3":"2575","WL_RPLC_POS_ifc_4":"3277","WL_RPLC_POS_ifc_5":"995","WL_RPLC_POS_WT_1":"33018","WL_RPLC_POS_WT_2":"32250","WL_RPLC_POS_WT_3":"38111","WL_RPLC_POS_WT_4":"30040","WL_RPLC_POS_WT_5":"32723"},{"Metabolite":"GlcCer d14:1/20:0","WL_RPLC_POS_ifc_1":"10806","WL_RPLC_POS_ifc_2":"11524","WL_RPLC_POS_ifc_3":"10623","WL_RPLC_POS_ifc_4":"9459","WL_RPLC_POS_ifc_5":"5023","WL_RPLC_POS_WT_1":"144339","WL_RPLC_POS_WT_2":"146565","WL_RPLC_POS_WT_3":"191335","WL_RPLC_POS_WT_4":"148590","WL_RPLC_POS_WT_5":"155146"},{"Metabolite":"GlcCer 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"Metabolites":[{"metabolite_name":"Cer 14:1;2O/18:0"},{"metabolite_name":"Cer 14:1;2O/20:0"},{"metabolite_name":"Cer 14:1;2O/24:0"},{"metabolite_name":"GlcCer d14:0/18:0"},{"metabolite_name":"GlcCer d14:0/20:0"},{"metabolite_name":"GlcCer d14:0/22:0"},{"metabolite_name":"GlcCer d14:0/24:0"},{"metabolite_name":"GlcCer d14:1/18:0"},{"metabolite_name":"GlcCer d14:1/20:0"},{"metabolite_name":"GlcCer d14:1/22:0"},{"metabolite_name":"CerPE d14:1;2O/16:0"},{"metabolite_name":"CerPE d14:1;2O/18:0"},{"metabolite_name":"CerPE d14:1;2O/20:0"},{"metabolite_name":"CerPE d14:1;2O/22:0"},{"metabolite_name":"CerPE d14:1;2O/24:0"},{"metabolite_name":"Cer 14:0;2O/18:0"},{"metabolite_name":"Cer 14:0;2O/20:0"},{"metabolite_name":"Cer 14:0;2O/24:0"},{"metabolite_name":"Lauroyl-CoA"},{"metabolite_name":"DG 16:1_18:3"},{"metabolite_name":"DG 18:1_18:1"},{"metabolite_name":"HexCer 14:0;2O/18:0"},{"metabolite_name":"HexCer 14:0;2O/20:0"},{"metabolite_name":"HexCer 14:0;2O/22:0"},{"metabolite_name":"LPE 34:1"},{"metabolite_name":"LPE 36:1"},{"metabolite_name":"LPE 36:3"},{"metabolite_name":"PC 13:0_22:3"},{"metabolite_name":"PC 14:1_20:2"},{"metabolite_name":"PC 14:1_22:4"},{"metabolite_name":"PC 17:0_17:0"},{"metabolite_name":"PC 17:2_17:2"},{"metabolite_name":"PC 18:1_18:1"},{"metabolite_name":"PC 18:2_18:2"},{"metabolite_name":"PC 18:3_18:3"},{"metabolite_name":"PE 10:0_26:3"},{"metabolite_name":"PE 14:1_20:2"},{"metabolite_name":"PE 17:0_17:0"},{"metabolite_name":"PE 17:2_17:2"},{"metabolite_name":"PE 18:0_18:5"},{"metabolite_name":"PE 18:1_18:1"},{"metabolite_name":"PE 18:2_18:2"},{"metabolite_name":"PS 18:0_18:3"},{"metabolite_name":"PS 18:1_18:1"},{"metabolite_name":"TG 14:1_16:1_18:2"},{"metabolite_name":"TG 16:0_16:0_16:1"},{"metabolite_name":"TG 16:0_16:1_18:2"},{"metabolite_name":"TG 16:0_18:1_18:1"},{"metabolite_name":"TG 16:1_16:1_18:2"},{"metabolite_name":"TG 16:1_16:1_18:3"},{"metabolite_name":"TG 16:1_18:2_18:2"},{"metabolite_name":"TG 16:1_18:2_18:3"},{"metabolite_name":"C14 Sphinganine"},{"metabolite_name":"C14 Sphinganine-1 phosphate"},{"metabolite_name":"C14 Sphingosine"}]
}

}