{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003166","ANALYSIS_ID":"AN005194","VERSION":"1","CREATED_ON":"April 11, 2024, 1:33 pm"},

"PROJECT":{"PROJECT_TITLE":"Releasing the mitochondrial respiration brake MCJ/DnaJC15 enhances CD8 CAR-T cell therapy efficacy","PROJECT_SUMMARY":"Metabolism of chimeric antigen receptor (CAR) T cells is emerging as an important area to improve CAR-T cell therapy in cancer treatment. Mitochondrial respiration is essential for survival and function of CAR-T cells, but developing strategies to specifically enhance mitochondrial respiration has been challenging. Here we identify MCJ/DnaJC15, an endogenous negative regulator of mitochondrial Complex I, as a metabolic target to enhance mitochondrial respiration in CD8 CAR-T cells. Loss of MCJ in CD8 CAR-T cells increases their in vitro and in vivo efficacy against mouse B cell leukemias. MCJ deficiency in TCR- specific CD8 cells also increases their efficacy against solid tumors in vivo. Furthermore, we reveal that human CD8 cells express MCJ and that silencing MCJ expression increases mitochondrial metabolism and anti-tumor activity of human CAR-T cells. Thus, targeting MCJ to enhance mitochondrial metabolism is a promising therapeutic strategy to improve the efficacy of adoptive T cell therapies.","INSTITUTE":"University of Colorado School of Medicine","LABORATORY":"Laboratory of Angelo D''Alessandro in collaboration with Mercedes Rincon","LAST_NAME":"Cendali","FIRST_NAME":"Francesca","ADDRESS":"13199 East Montview Boulevard, Aurora, CO, 80045, USA","EMAIL":"francesca.cendali@cuanschutz.edu","PHONE":"3037246131"},

"STUDY":{"STUDY_TITLE":"Metabolomics of Murine WT or MCJ KO CD19-BBz CD8 CAR-T cells","STUDY_SUMMARY":"MCJ/DnaJC15 is an endogenous negative regulator of Complex I and mitochondrial respiration. The goal of these experiments are to characterize the metabolic profile of murine WT or MCJ KO CD8 CD19-BBz CAR-T cells after 3 expansions (6 days) with IL-2 (60IU/ml).","INSTITUTE":"University of Colorado School of Medicine","LABORATORY":"Laboratory of Angelo D''Alessandro in collaboration with Mercedes Rincon","LAST_NAME":"Cendali","FIRST_NAME":"Francesca","ADDRESS":"13199 East Montview Boulevard, Aurora, CO, 80045, USA","EMAIL":"francesca.cendali@cuanschutz.edu","PHONE":"3037246131"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"WT CD19-BBz CART_1",
"Factors":{"factor":"WT","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"1"}
},
{
"Subject ID":"-",
"Sample ID":"WT CD19-BBz CART_2",
"Factors":{"factor":"WT","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"2"}
},
{
"Subject ID":"-",
"Sample ID":"WT CD19-BBz CART_3",
"Factors":{"factor":"WT","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"3"}
},
{
"Subject ID":"-",
"Sample ID":"WT CD19-BBz CART_4",
"Factors":{"factor":"WT","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"4"}
},
{
"Subject ID":"-",
"Sample ID":"MCJ KO CD19-BBz CART_1",
"Factors":{"factor":"MCJ KO","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"5"}
},
{
"Subject ID":"-",
"Sample ID":"MCJ KO CD19-BBz CART_2",
"Factors":{"factor":"MCJ KO","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"6"}
},
{
"Subject ID":"-",
"Sample ID":"MCJ KO CD19-BBz CART_3",
"Factors":{"factor":"MCJ KO","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"7"}
},
{
"Subject ID":"-",
"Sample ID":"MCJ KO CD19-BBz CART_4",
"Factors":{"factor":"MCJ KO","Sample source":"T-Cells"},
"Additional sample data":{"RAW_FILE_NAME(Raw File)":"8"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mouse CD8 cells were isolated with negative selection as described above, activated with anti-CD3/anti-CD28 beads (Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation, Gibco, Cat#11453D) with addition of recombinant human IL-2 (rhIL-2) (60 IU/ml) and human recombinant IL-7 (10 ng/ml). On the second and third day of activation, the supernatant containing the CD19-BBz CAR/hEGFR retrovirus was spun on a plate coated with retronectin (Takara Bio Inc.) (2000xg for 2.5h at 32C). The activated cells were added to the viral-coated plate for transduction. The transduced CD8 CAR-T cells were removed from the beads on the fourth day and expanded with rhIL-2 (60 IU/ml) or rhIL-7 (10 ng/ml) and rhL-15 (100 ng/ml). After 2 days (1st expansion) cells were split 1/3 with fresh cytokine-containing medium. After 2 more days (2nd expansion), cells were split again with fresh cytokine-containing medium. Two days later (3rd expansion) cells were used for experiment or incubated in cytokine free-medium. For CAR+ cells enrichment, the CAR-T cells expanded with IL-2 for 3 expansions were incubated with an anti-hEGFR-PE (BioLegend, Cat#352904, RRID: AB_10896794) antibody, followed by incubation with anti-PE microbeads (Miltenyi, order#130-048-801), and purified using the Miltenyi LS columns as recommended by the manufacturer (Miltenyi). The enrichment resulted in a 98-100% CAR+ population, as verified by flow cytometry.","SAMPLE_TYPE":"T-cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Metabolomics differences in this experiment are observed at baseline"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"CAR-T cells were isolated as described above either from in vitro culture or from bone marrow harvested in an in vivo study. The cells were washed in PBS and frozen at -80C until the assay is ready to run. Metabolites from cells were extracted at 2x106 cells/ml at 4°C (30 min) in the presence of 5:3:2 MeOH:MeCN:water (v/v/v). The samples were spun down and the resulting supernatant was transferred to new tubes and dried under a vacuum. The resulting residue was reconstituted in 0.1% formic acid at a 3x concentration, then analyzed on a Thermo Vanquish UHPLC coupled to a Thermo Q Exactive MS as previously described in detail."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Negative ion Mode","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)","SOLVENT_A":"95% water/5% acetonitrile; 1 mM ammonium acetate","SOLVENT_B":"95% acetonitrile/5% water; 1 mM ammonium acetate","FLOW_GRADIENT":"0-0.5 min 0% B, 0.5-1.1 min 0-100% B, 1.1-2.75 min hold at 100% B, 2.75-3 min 100-0% B, 3-5 min hold at 0% B","FLOW_RATE":"0.450ml/min","COLUMN_TEMPERATURE":"45"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Resolution 70,000, scan range 65-900 m/z, maximum injection time 200 ms, microscans 2, automatic gain control (AGC) 3 x 10^6 ions, source voltage 4.0 kV, capillary temperature 320 C, and sheath gas 45, auxiliary gas 15, and sweep gas 0 (all nitrogen). Data converted to mzXML using RawConverter. Metabolites were annotated and integrated using Maven in conjunction with the KEGG database."},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"ATP","WT CD19-BBz CART_1":"41731.19","WT CD19-BBz CART_2":"6428.767","WT CD19-BBz CART_3":"24878.19","WT CD19-BBz CART_4":"35170.29","MCJ KO CD19-BBz CART_1":"79503.05","MCJ KO CD19-BBz CART_2":"81745.34","MCJ KO CD19-BBz CART_3":"138619.4","MCJ KO CD19-BBz CART_4":"85962.03","":""},{"Metabolite":"CDP","WT CD19-BBz CART_1":"53948","WT CD19-BBz CART_2":"47685.77","WT CD19-BBz CART_3":"46709.7","WT CD19-BBz CART_4":"53225.76","MCJ KO CD19-BBz CART_1":"83453.38","MCJ KO CD19-BBz CART_2":"80334.26","MCJ KO CD19-BBz CART_3":"79512.63","MCJ KO CD19-BBz CART_4":"66829.29","":""},{"Metabolite":"dTMP","WT CD19-BBz CART_1":"11528.27","WT CD19-BBz CART_2":"13090.34","WT CD19-BBz CART_3":"15471.74","WT CD19-BBz CART_4":"12250.69","MCJ KO CD19-BBz CART_1":"8103.299","MCJ KO CD19-BBz CART_2":"10143.27","MCJ KO CD19-BBz CART_3":"9107.273","MCJ KO CD19-BBz CART_4":"12798.74","":""},{"Metabolite":"UTP","WT CD19-BBz CART_1":"33895.8","WT CD19-BBz CART_2":"14083.71","WT CD19-BBz CART_3":"21179.4","WT CD19-BBz CART_4":"29137.43","MCJ KO CD19-BBz CART_1":"79893.7","MCJ KO CD19-BBz CART_2":"80497.16","MCJ KO CD19-BBz CART_3":"85839.19","MCJ KO CD19-BBz CART_4":"48459.85","":""},{"Metabolite":"4-Pyridoxate","WT CD19-BBz CART_1":"53052.21","WT CD19-BBz CART_2":"36336.4","WT CD19-BBz CART_3":"38370.96","WT CD19-BBz CART_4":"40768.04","MCJ KO CD19-BBz CART_1":"53107.3","MCJ KO CD19-BBz CART_2":"51397.92","MCJ KO CD19-BBz CART_3":"67147.98","MCJ KO CD19-BBz CART_4":"73606.19","":""},{"Metabolite":"UDP-glucose","WT CD19-BBz CART_1":"109945.5","WT CD19-BBz CART_2":"102305","WT CD19-BBz CART_3":"106772.5","WT CD19-BBz CART_4":"117610.4","MCJ KO CD19-BBz CART_1":"173604.3","MCJ KO CD19-BBz CART_2":"171099.8","MCJ KO CD19-BBz CART_3":"153633.5","MCJ KO CD19-BBz CART_4":"137282.2","":""},{"Metabolite":"ADP-D-ribose","WT CD19-BBz CART_1":"80313.99","WT CD19-BBz CART_2":"39212.17","WT CD19-BBz CART_3":"48370.59","WT CD19-BBz CART_4":"37083.43","MCJ KO CD19-BBz CART_1":"26234.21","MCJ KO CD19-BBz CART_2":"54659.27","MCJ KO CD19-BBz CART_3":"40937.18","MCJ KO CD19-BBz CART_4":"57243.11","":""},{"Metabolite":"Phosphate","WT CD19-BBz CART_1":"14296980","WT CD19-BBz CART_2":"8234016","WT CD19-BBz CART_3":"13455610","WT CD19-BBz CART_4":"11907920","MCJ KO CD19-BBz CART_1":"12452870","MCJ KO CD19-BBz CART_2":"12267700","MCJ KO CD19-BBz CART_3":"13097670","MCJ KO CD19-BBz CART_4":"11994820","":""},{"Metabolite":"Diphosphate","WT CD19-BBz CART_1":"1350856","WT CD19-BBz CART_2":"888204.6","WT CD19-BBz CART_3":"1270134","WT CD19-BBz CART_4":"1227957","MCJ KO CD19-BBz CART_1":"1829761","MCJ KO CD19-BBz CART_2":"1288761","MCJ KO CD19-BBz CART_3":"1300646","MCJ KO CD19-BBz CART_4":"1350158","":""},{"Metabolite":"D-Glucose","WT CD19-BBz CART_1":"34903.93","WT CD19-BBz CART_2":"33176.79","WT CD19-BBz CART_3":"52589.67","WT CD19-BBz CART_4":"54858.92","MCJ KO CD19-BBz CART_1":"37811.08","MCJ KO CD19-BBz CART_2":"55359.25","MCJ KO CD19-BBz CART_3":"37016.07","MCJ KO CD19-BBz CART_4":"37469.9","":""},{"Metabolite":"D-Glucose 6/1-phosphate","WT CD19-BBz CART_1":"85955.66","WT CD19-BBz CART_2":"67637.87","WT CD19-BBz CART_3":"52940.54","WT CD19-BBz CART_4":"77000.13","MCJ KO CD19-BBz CART_1":"111030.3","MCJ KO CD19-BBz CART_2":"77257.45","MCJ KO CD19-BBz CART_3":"78838.41","MCJ KO CD19-BBz CART_4":"79792.44","":""},{"Metabolite":"D-Glyceraldehyde 3-phosphate/Glycerone phosphate","WT CD19-BBz CART_1":"287686","WT CD19-BBz CART_2":"343037.8","WT CD19-BBz CART_3":"372692.3","WT CD19-BBz CART_4":"339469.2","MCJ KO CD19-BBz CART_1":"438925.6","MCJ KO CD19-BBz CART_2":"401145.6","MCJ KO CD19-BBz CART_3":"437910.4","MCJ KO CD19-BBz CART_4":"360800.9","":""},{"Metabolite":"2/3-Phospho-D-glycerate","WT CD19-BBz CART_1":"20790.25","WT CD19-BBz CART_2":"13434.66","WT CD19-BBz CART_3":"14234.36","WT CD19-BBz CART_4":"19509.62","MCJ KO CD19-BBz CART_1":"38532.4","MCJ KO CD19-BBz CART_2":"33113.87","MCJ KO CD19-BBz CART_3":"25507.95","MCJ KO CD19-BBz CART_4":"28076.16","":""},{"Metabolite":"Pyruvate","WT CD19-BBz CART_1":"57872.79","WT CD19-BBz CART_2":"52969.32","WT CD19-BBz CART_3":"45175.14","WT CD19-BBz CART_4":"60090.41","MCJ KO CD19-BBz CART_1":"53257.15","MCJ KO CD19-BBz CART_2":"50325.11","MCJ KO CD19-BBz CART_3":"74337.44","MCJ KO CD19-BBz CART_4":"49558.11","":""},{"Metabolite":"Lactate","WT CD19-BBz CART_1":"4848126","WT CD19-BBz CART_2":"3885394","WT CD19-BBz CART_3":"5318762","WT CD19-BBz CART_4":"5787558","MCJ KO CD19-BBz CART_1":"5375694","MCJ KO CD19-BBz CART_2":"5242856","MCJ KO CD19-BBz CART_3":"6198282","MCJ KO CD19-BBz CART_4":"5424948","":""},{"Metabolite":"Maltose","WT CD19-BBz CART_1":"21164.09","WT CD19-BBz CART_2":"15210.54","WT CD19-BBz CART_3":"15643.46","WT CD19-BBz CART_4":"24864.53","MCJ KO CD19-BBz CART_1":"10121.72","MCJ KO CD19-BBz CART_2":"13867.65","MCJ KO CD19-BBz CART_3":"27094.81","MCJ KO CD19-BBz CART_4":"16564.82","":""},{"Metabolite":"Mannitol/Sorbitol","WT CD19-BBz CART_1":"40654.9","WT CD19-BBz CART_2":"41214.98","WT CD19-BBz CART_3":"40424.16","WT CD19-BBz CART_4":"39911.29","MCJ KO CD19-BBz CART_1":"52945.3","MCJ KO CD19-BBz CART_2":"50903.8","MCJ KO CD19-BBz CART_3":"36894.93","MCJ KO CD19-BBz CART_4":"35577.58","":""},{"Metabolite":"Citrate","WT CD19-BBz CART_1":"618699.8","WT CD19-BBz CART_2":"191719","WT CD19-BBz CART_3":"289149.5","WT CD19-BBz CART_4":"357374","MCJ KO CD19-BBz CART_1":"404766.8","MCJ KO CD19-BBz CART_2":"414949.8","MCJ KO CD19-BBz CART_3":"642812.4","MCJ KO CD19-BBz CART_4":"595414.9","":""},{"Metabolite":"2-Oxoglutarate","WT CD19-BBz CART_1":"12281.25","WT CD19-BBz CART_2":"15227.17","WT CD19-BBz CART_3":"15536.72","WT CD19-BBz CART_4":"18042.83","MCJ KO CD19-BBz CART_1":"28747.53","MCJ KO CD19-BBz CART_2":"23252.08","MCJ KO CD19-BBz CART_3":"33593.17","MCJ KO CD19-BBz CART_4":"32512.49","":""},{"Metabolite":"Succinate","WT CD19-BBz CART_1":"138719.9","WT CD19-BBz CART_2":"129564","WT CD19-BBz CART_3":"173048.6","WT CD19-BBz CART_4":"147926.4","MCJ KO CD19-BBz CART_1":"174430.2","MCJ KO CD19-BBz CART_2":"178328.8","MCJ KO CD19-BBz CART_3":"205234.1","MCJ KO CD19-BBz CART_4":"244900","":""},{"Metabolite":"Fumarate","WT CD19-BBz CART_1":"155572.1","WT CD19-BBz CART_2":"90909.04","WT CD19-BBz CART_3":"124985.8","WT CD19-BBz CART_4":"130289.6","MCJ KO CD19-BBz CART_1":"129184.5","MCJ KO CD19-BBz CART_2":"166935.8","MCJ KO CD19-BBz CART_3":"179206.8","MCJ KO CD19-BBz CART_4":"177238.2","":""},{"Metabolite":"Malate","WT CD19-BBz CART_1":"328446.8","WT CD19-BBz CART_2":"247421.1","WT CD19-BBz CART_3":"218096.7","WT CD19-BBz CART_4":"297673.4","MCJ KO CD19-BBz CART_1":"325895.5","MCJ KO CD19-BBz CART_2":"336860.8","MCJ KO CD19-BBz CART_3":"453008.4","MCJ KO CD19-BBz CART_4":"273032.2","":""},{"Metabolite":"2-Hydroxyglutarate/Citramalate","WT CD19-BBz CART_1":"39109.5","WT CD19-BBz CART_2":"40886.45","WT CD19-BBz CART_3":"46718.08","WT CD19-BBz CART_4":"44507.89","MCJ KO CD19-BBz CART_1":"74731.09","MCJ KO CD19-BBz CART_2":"79205.07","MCJ KO CD19-BBz CART_3":"82418.81","MCJ KO CD19-BBz CART_4":"77209.23","":""},{"Metabolite":"Sedoheptulose 1/7-phosphate","WT CD19-BBz CART_1":"40664.46","WT CD19-BBz CART_2":"38187.89","WT CD19-BBz CART_3":"35360.77","WT CD19-BBz CART_4":"34914.26","MCJ KO CD19-BBz CART_1":"50929.48","MCJ KO CD19-BBz CART_2":"34809.28","MCJ KO CD19-BBz CART_3":"41409.61","MCJ KO CD19-BBz CART_4":"46787.02","":""},{"Metabolite":"alpha-D-Ribose 1-phosphate","WT CD19-BBz CART_1":"169594.8","WT CD19-BBz CART_2":"125331.4","WT CD19-BBz CART_3":"116209","WT CD19-BBz CART_4":"120834.6","MCJ KO CD19-BBz CART_1":"142507.4","MCJ KO CD19-BBz CART_2":"122830.7","MCJ KO CD19-BBz CART_3":"135970.8","MCJ KO CD19-BBz CART_4":"164456.7","":""},{"Metabolite":"Dimethylglycine","WT CD19-BBz CART_1":"183660.9","WT CD19-BBz CART_2":"121348.4","WT CD19-BBz CART_3":"160857.4","WT CD19-BBz CART_4":"152375.3","MCJ KO CD19-BBz CART_1":"145719","MCJ KO CD19-BBz CART_2":"193498.6","MCJ KO CD19-BBz CART_3":"185511.5","MCJ KO CD19-BBz CART_4":"220374.2","":""},{"Metabolite":"N-Acetylneuraminate","WT CD19-BBz CART_1":"122704.1","WT CD19-BBz CART_2":"62105.49","WT CD19-BBz CART_3":"62626.44","WT CD19-BBz CART_4":"68726.91","MCJ KO CD19-BBz CART_1":"81198.21","MCJ KO CD19-BBz CART_2":"100110.1","MCJ KO CD19-BBz CART_3":"107409.4","MCJ KO CD19-BBz CART_4":"115418.2","":""},{"Metabolite":"N-Glycoloyl-neuraminate","WT CD19-BBz CART_1":"249265.9","WT CD19-BBz CART_2":"173564","WT CD19-BBz CART_3":"179054.4","WT CD19-BBz CART_4":"179983","MCJ KO CD19-BBz CART_1":"232159.2","MCJ KO CD19-BBz CART_2":"201731.4","MCJ KO CD19-BBz CART_3":"225946.6","MCJ KO CD19-BBz CART_4":"213605.1","":""},{"Metabolite":"UDP-N-acetyl-D-glucosamine","WT CD19-BBz CART_1":"229337.2","WT CD19-BBz CART_2":"207586.8","WT CD19-BBz CART_3":"248297.6","WT CD19-BBz CART_4":"229223","MCJ KO CD19-BBz CART_1":"367094","MCJ KO CD19-BBz CART_2":"355713.3","MCJ KO CD19-BBz CART_3":"327184.5","MCJ KO CD19-BBz CART_4":"301008.9","":""},{"Metabolite":"Phosphocreatine","WT CD19-BBz CART_1":"12416.68","WT CD19-BBz CART_2":"15688.38","WT CD19-BBz CART_3":"12999.27","WT CD19-BBz CART_4":"13079.56","MCJ KO CD19-BBz CART_1":"20042.31","MCJ KO CD19-BBz CART_2":"18910.34","MCJ KO CD19-BBz CART_3":"17525.09","MCJ KO CD19-BBz CART_4":"14660.42","":""},{"Metabolite":"Sphinganine 1-phosphate","WT CD19-BBz CART_1":"7634.625","WT CD19-BBz CART_2":"6593.951","WT CD19-BBz CART_3":"11418.5","WT CD19-BBz CART_4":"9275.2","MCJ KO CD19-BBz CART_1":"6087.835","MCJ KO CD19-BBz CART_2":"6923.96","MCJ KO CD19-BBz CART_3":"8480.534","MCJ KO CD19-BBz CART_4":"9538.961","":""}],

"Metabolites":[{"Metabolite":"ATP","CmpdID":"C00002","parent":"505.99","medRt":"0.609"},{"Metabolite":"CDP","CmpdID":"C00112","parent":"402.01","medRt":"0.632"},{"Metabolite":"dTMP","CmpdID":"C00364","parent":"321.05","medRt":"0.929"},{"Metabolite":"UTP","CmpdID":"C00075","parent":"482.96","medRt":"0.630"},{"Metabolite":"4-Pyridoxate","CmpdID":"C00847","parent":"182.04","medRt":"0.716"},{"Metabolite":"UDP-glucose","CmpdID":"C00029","parent":"565.05","medRt":"0.620"},{"Metabolite":"ADP-D-ribose","CmpdID":"C01882","parent":"558.06","medRt":"0.646"},{"Metabolite":"Phosphate","CmpdID":"C00009","parent":"96.97","medRt":"0.670"},{"Metabolite":"Diphosphate","CmpdID":"C00013","parent":"176.93","medRt":"0.613"},{"Metabolite":"D-Glucose","CmpdID":"C00031","parent":"179.06","medRt":"0.691"},{"Metabolite":"D-Glucose 6/1-phosphate","CmpdID":"C02965","parent":"259.02","medRt":"0.637"},{"Metabolite":"D-Glyceraldehyde 3-phosphate/Glycerone phosphate","CmpdID":"C00118","parent":"168.99","medRt":"1.818"},{"Metabolite":"2/3-Phospho-D-glycerate","CmpdID":"C00631","parent":"184.98","medRt":"0.615"},{"Metabolite":"Pyruvate","CmpdID":"C00022","parent":"87.01","medRt":"0.811"},{"Metabolite":"Lactate","CmpdID":"C01432","parent":"89.02","medRt":"0.789"},{"Metabolite":"Maltose","CmpdID":"C00208","parent":"341.11","medRt":"0.729"},{"Metabolite":"Mannitol/Sorbitol","CmpdID":"C00392","parent":"181.07","medRt":"0.699"},{"Metabolite":"Citrate","CmpdID":"C00158","parent":"191.02","medRt":"0.627"},{"Metabolite":"2-Oxoglutarate","CmpdID":"C00026","parent":"145.01","medRt":"0.807"},{"Metabolite":"Succinate","CmpdID":"C00042","parent":"117.02","medRt":"0.808"},{"Metabolite":"Fumarate","CmpdID":"C00122","parent":"108.24","medRt":"0.628"},{"Metabolite":"Malate","CmpdID":"C00149","parent":"133.01","medRt":"0.612"},{"Metabolite":"2-Hydroxyglutarate/Citramalate","CmpdID":"C02630","parent":"147.03","medRt":"0.811"},{"Metabolite":"Sedoheptulose 1/7-phosphate","CmpdID":"C06222","parent":"289.03","medRt":"0.636"},{"Metabolite":"alpha-D-Ribose 1-phosphate","CmpdID":"C00620","parent":"229.01","medRt":"0.637"},{"Metabolite":"Dimethylglycine","CmpdID":"C01026","parent":"102.05","medRt":"0.639"},{"Metabolite":"N-Acetylneuraminate","CmpdID":"C00270","parent":"308.10","medRt":"0.646"},{"Metabolite":"N-Glycoloyl-neuraminate","CmpdID":"C03410","parent":"324.09","medRt":"0.643"},{"Metabolite":"UDP-N-acetyl-D-glucosamine","CmpdID":"C00043","parent":"606.07","medRt":"0.636"},{"Metabolite":"Phosphocreatine","CmpdID":"C02305","parent":"210.03","medRt":"0.622"},{"Metabolite":"Sphinganine 1-phosphate","CmpdID":"C01120","parent":"380.26","medRt":"3.706"}]
}

}