{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003173","ANALYSIS_ID":"AN005207","VERSION":"1","CREATED_ON":"April 17, 2024, 7:41 am"},

"PROJECT":{"PROJECT_TITLE":"Assessment and partial characterization of candidate genes in dihydrochalcone and arbutin biosynthesis in an apple-pear hybrid by de novo transcriptome assembly","PROJECT_SUMMARY":"The goal of the study was to determine the phenolic profile of young and old leaves, as well as fruit of apple (Malus x domestica), pear (Pyrus communis) and an intergeneric apple-pear hybrid. Three independent replicates were obtained for each genotype from the germplasm collection at Fondazione Edmund Mach (Italy) and analyzed by a phenolic targeted LC/MS-MS method. In addition, candidate genes retrieved from a de novo transcriptome assembly were tested in recombinant proteins (n = 3) to determine the conversion of hydroquinone to arbutin. Combining RNA-Seq, in silico functional annotation prediction, targeted gene expression analysis and expression – metabolite correlations with the data submitted to Metabolomics Workbench, we identified candidate genes for functional characterisation, resulting in the identification of active arbutin synthases in the hybrid and parental genotypes. We found that the putative arbutin synthases of pear (PcAS) and apple-pear hybrid (HybAS) were able to convert hydroquinone into arbutin. Interestingly, also one out of two putative arbutin synthases isolated from apple (MdAS1) could produce arbutin in vitro. However, the metabolomic profiling of phenolic compounds showed that apple lacks of arbutin and was found to accumulate the precursor hydroquinone in traces in young and old leaves of apple. Although quercetin was accumulated in similar amounts in the same tissues, a luminiscence-based assay showed that quercetin was converted only 25% compared to activity towards hydroquinone in the tested conditions. In summary, the metabolomic profiling submitted to Metabolomics workbench also shows that: 1) arbutin is accumulated mainly in young leaves of pear, followed by the apple-pear hybrid and was found in traces in apple fruit; 2) rutin was found mainly in pear and apple-pear hybrid tissues; 3) phenolic profile of apple is dominated by phloridzin and undetectable in all pear tissues analyzed, with young leaves being the tissue showing highest accumulation.","INSTITUTE":"Fondazione Edmund Mach","LAST_NAME":"Miranda Chavez","FIRST_NAME":"Simon David","ADDRESS":"Via Mach, 1, San Michele all''Adige, Trento, 38098, Italy","EMAIL":"simondavid.mirandachavez@fmach.it","PHONE":"+390461615231"},

"STUDY":{"STUDY_TITLE":"Assessment and partial characterization of candidate genes in dihydrochalcone and arbutin biosynthesis in an apple-pear hybrid by de novo transcriptome assembly","STUDY_SUMMARY":"The goal of the study was to determine the phenolic profile of young and old leaves, as well as fruit of apple (Malus x domestica), pear (Pyrus communis) and an intergeneric apple-pear hybrid. Three independent replicates were obtained for each genotype from the germplasm collection at Fondazione Edmund Mach (Italy) and analyzed by a targeted phenolic LC/MS-MS method. In addition, candidate genes from apple, pear and apple-pear hybrid retrieved from a de novo transcriptome assembly were expressed in E. coli and recombinant proteins were tested (in triplicate) to determine the conversion of hydroquinone to arbutin. Combining RNA-Seq, in silico functional annotation prediction, targeted gene expression analysis and expression – metabolite correlations with the data submitted to Metabolomics Workbench, we identified candidate genes for functional characterisation, resulting in the identification of active arbutin synthases in the hybrid and parental genotypes. We found that the putative arbutin synthases of pear (PcAS) and apple-pear hybrid (HybAS) were able to convert hydroquinone into arbutin. Interestingly, also one out of two putative arbutin synthases isolated from apple (MdAS1) could produce arbutin in vitro. However, the metabolomic profiling of phenolic compounds showed that apple lacks of arbutin and was found to accumulate the precursor hydroquinone in traces in young and old leaves of apple. Although quercetin was accumulated in similar amounts in the same tissues, a luminiscence-based assay showed that quercetin was converted only 25% compared to activity towards hydroquinone in the tested conditions. In summary, the metabolomic profiling submitted to Metabolomics workbench also shows that: 1) arbutin is accumulated mainly in young leaves of pear, followed by the apple-pear hybrid and was found in traces in apple fruit; 2) rutin was found mainly in pear and apple-pear hybrid tissues; 3) phenolic profile of apple is dominated by phloridzin and undetectable in all pear tissues analyzed, with young leaves being the tissue showing highest accumulation.","INSTITUTE":"Fondazione Edmund Mach","LAST_NAME":"Miranda Chavez","FIRST_NAME":"Simon David","ADDRESS":"Via Mach, 1, San Michele all''Adige, Trento, 38098, Italy","EMAIL":"simondavid.mirandachavez@fmach.it","PHONE":"+390461615231"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Malus domestica/Pyrus communis/Apple-pear intergeneric hybrid"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Young Leaf_Malus domestica_YLR1",
"Sample ID":"Young Leaf_Malus domestica_YLR1",
"Factors":{"Sample source":"Young Leaf","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR1","RAW_FILE_NAME":"Apple_YoungLeaves_R1.mzML"}
},
{
"Subject ID":"Young Leaf_Malus domestica_YLR2",
"Sample ID":"Young Leaf_Malus domestica_YLR2",
"Factors":{"Sample source":"Young Leaf","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR2","RAW_FILE_NAME":"Apple_YoungLeaves_R2.mzML"}
},
{
"Subject ID":"Young Leaf_Malus domestica_YLR3",
"Sample ID":"Young Leaf_Malus domestica_YLR3",
"Factors":{"Sample source":"Young Leaf","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR3","RAW_FILE_NAME":"Apple_YoungLeaves_R3.mzML"}
},
{
"Subject ID":"Old Leaf_Malus domestica_OLR1",
"Sample ID":"Old Leaf_Malus domestica_OLR1",
"Factors":{"Sample source":"Old Leaf","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR1","RAW_FILE_NAME":"Apple_OldLeaves_R1.mzML"}
},
{
"Subject ID":"Old Leaf_Malus domestica_OLR2",
"Sample ID":"Old Leaf_Malus domestica_OLR2",
"Factors":{"Sample source":"Old Leaf","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR2","RAW_FILE_NAME":"Apple_OldLeaves_R2.mzML"}
},
{
"Subject ID":"Old Leaf_Malus domestica_OLR3",
"Sample ID":"Old Leaf_Malus domestica_OLR3",
"Factors":{"Sample source":"Old Leaf","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR3","RAW_FILE_NAME":"Apple_OldLeaves_R3.mzML"}
},
{
"Subject ID":"Fruit_Malus domestica_FR1",
"Sample ID":"Fruit_Malus domestica_FR1",
"Factors":{"Sample source":"Fruit","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"FR1","RAW_FILE_NAME":"Apple_Fruit_R1.mzML"}
},
{
"Subject ID":"Fruit_Malus domestica_FR2",
"Sample ID":"Fruit_Malus domestica_FR2",
"Factors":{"Sample source":"Fruit","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"FR2","RAW_FILE_NAME":"Apple_Fruit_R2.mzML"}
},
{
"Subject ID":"Fruit_Malus domestica_FR3",
"Sample ID":"Fruit_Malus domestica_FR3",
"Factors":{"Sample source":"Fruit","Genotype":"Malus domestica","Treatment":"Control"},
"Additional sample data":{"Batch":"FR3","RAW_FILE_NAME":"Apple_Fruit_R3.mzML"}
},
{
"Subject ID":"Young Leaf_Pyrus communis_YLR1",
"Sample ID":"Young Leaf_Pyrus communis_YLR1",
"Factors":{"Sample source":"Young Leaf","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR1","RAW_FILE_NAME":"Pear_YoungLeaves_R1.mzML"}
},
{
"Subject ID":"Young Leaf_Pyrus communis_YLR2",
"Sample ID":"Young Leaf_Pyrus communis_YLR2",
"Factors":{"Sample source":"Young Leaf","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR2","RAW_FILE_NAME":"Pear_YoungLeaves_R2.mzML"}
},
{
"Subject ID":"Young Leaf_Pyrus communis_YLR3",
"Sample ID":"Young Leaf_Pyrus communis_YLR3",
"Factors":{"Sample source":"Young Leaf","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR3","RAW_FILE_NAME":"Pear_YoungLeaves_R3.mzML"}
},
{
"Subject ID":"Old Leaf_Pyrus communis_OLR1",
"Sample ID":"Old Leaf_Pyrus communis_OLR1",
"Factors":{"Sample source":"Old Leaf","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR1","RAW_FILE_NAME":"Pear_OldLeaves_R1.mzML"}
},
{
"Subject ID":"Old Leaf_Pyrus communis_OLR2",
"Sample ID":"Old Leaf_Pyrus communis_OLR2",
"Factors":{"Sample source":"Old Leaf","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR2","RAW_FILE_NAME":"Pear_OldLeaves_R2.mzML"}
},
{
"Subject ID":"Old Leaf_Pyrus communis_OLR3",
"Sample ID":"Old Leaf_Pyrus communis_OLR3",
"Factors":{"Sample source":"Old Leaf","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR3","RAW_FILE_NAME":"Pear_OldLeaves_R3.mzML"}
},
{
"Subject ID":"Fruit_Pyrus communis_FR1",
"Sample ID":"Fruit_Pyrus communis_FR1",
"Factors":{"Sample source":"Fruit","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"FR1","RAW_FILE_NAME":"Pear_Fruit_R1.mzML"}
},
{
"Subject ID":"Fruit_Pyrus communis_FR2",
"Sample ID":"Fruit_Pyrus communis_FR2",
"Factors":{"Sample source":"Fruit","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"FR2","RAW_FILE_NAME":"Pear_Fruit_R2.mzML"}
},
{
"Subject ID":"Fruit_Pyrus communis_FR3",
"Sample ID":"Fruit_Pyrus communis_FR3",
"Factors":{"Sample source":"Fruit","Genotype":"Pyrus communis","Treatment":"Control"},
"Additional sample data":{"Batch":"FR3","RAW_FILE_NAME":"Pear_Fruit_R3.mzML"}
},
{
"Subject ID":"Young Leaf_Apple pear hybrid_YLR1",
"Sample ID":"Young Leaf_Apple pear hybrid_YLR1",
"Factors":{"Sample source":"Young Leaf","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR1","RAW_FILE_NAME":"Hybrid_YoungLeaves_R1.mzML"}
},
{
"Subject ID":"Young Leaf_Apple pear hybrid_YLR2",
"Sample ID":"Young Leaf_Apple pear hybrid_YLR2",
"Factors":{"Sample source":"Young Leaf","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR2","RAW_FILE_NAME":"Hybrid_YoungLeaves_R2.mzML"}
},
{
"Subject ID":"Young Leaf_Apple pear hybrid_YLR3",
"Sample ID":"Young Leaf_Apple pear hybrid_YLR3",
"Factors":{"Sample source":"Young Leaf","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"YLR3","RAW_FILE_NAME":"Hybrid_YoungLeaves_R3.mzML"}
},
{
"Subject ID":"Old Leaf_Apple pear hybrid_OLR1",
"Sample ID":"Old Leaf_Apple pear hybrid_OLR1",
"Factors":{"Sample source":"Old Leaf","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR1","RAW_FILE_NAME":"Hybrid_OldLeaves_R1.mzML"}
},
{
"Subject ID":"Old Leaf_Apple pear hybrid_OLR2",
"Sample ID":"Old Leaf_Apple pear hybrid_OLR2",
"Factors":{"Sample source":"Old Leaf","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR2","RAW_FILE_NAME":"Hybrid_OldLeaves_R2.mzML"}
},
{
"Subject ID":"Old Leaf_Apple pear hybrid_OLR3",
"Sample ID":"Old Leaf_Apple pear hybrid_OLR3",
"Factors":{"Sample source":"Old Leaf","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"OLR3","RAW_FILE_NAME":"Hybrid_OldLeaves_R3.mzML"}
},
{
"Subject ID":"Fruit_Apple pear hybrid_FR1",
"Sample ID":"Fruit_Apple pear hybrid_FR1",
"Factors":{"Sample source":"Fruit","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"FR1","RAW_FILE_NAME":"Hybrid_Fruit_R1.mzML"}
},
{
"Subject ID":"Fruit_Apple pear hybrid_FR2",
"Sample ID":"Fruit_Apple pear hybrid_FR2",
"Factors":{"Sample source":"Fruit","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"FR2","RAW_FILE_NAME":"Hybrid_Fruit_R2.mzML"}
},
{
"Subject ID":"Fruit_Apple pear hybrid_FR3",
"Sample ID":"Fruit_Apple pear hybrid_FR3",
"Factors":{"Sample source":"Fruit","Genotype":"Apple pear hybrid","Treatment":"Control"},
"Additional sample data":{"Batch":"FR3","RAW_FILE_NAME":"Hybrid_Fruit_R3.mzML"}
},
{
"Subject ID":"Enzyme assay_Malus domestica_MdAS1R1",
"Sample ID":"Enzyme assay_Malus domestica_MdAS1R1",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Malus domestica","Treatment":"5ug"},
"Additional sample data":{"Batch":"MdAS1R1","RAW_FILE_NAME":"MdAS1_R1.mzML"}
},
{
"Subject ID":"Enzyme assay_Malus domestica_MdAS1R2",
"Sample ID":"Enzyme assay_Malus domestica_MdAS1R2",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Malus domestica","Treatment":"5ug"},
"Additional sample data":{"Batch":"MdAS1R2","RAW_FILE_NAME":"MdAS1_R2.mzML"}
},
{
"Subject ID":"Enzyme assay_Malus domestica_MdAS1R3",
"Sample ID":"Enzyme assay_Malus domestica_MdAS1R3",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Malus domestica","Treatment":"5ug"},
"Additional sample data":{"Batch":"MdAS1R3","RAW_FILE_NAME":"MdAS1_R3.mzML"}
},
{
"Subject ID":"Enzyme assay_Malus domestica_MdAS2R1",
"Sample ID":"Enzyme assay_Malus domestica_MdAS2R1",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Malus domestica","Treatment":"5ug"},
"Additional sample data":{"Batch":"MdAS2R1","RAW_FILE_NAME":"MdAS2_R1.mzML"}
},
{
"Subject ID":"Enzyme assay_Malus domestica_MdAS2R2",
"Sample ID":"Enzyme assay_Malus domestica_MdAS2R2",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Malus domestica","Treatment":"5ug"},
"Additional sample data":{"Batch":"MdAS2R2","RAW_FILE_NAME":"MdAS2_R2.mzML"}
},
{
"Subject ID":"Enzyme assay_Malus domestica_MdAS2R3",
"Sample ID":"Enzyme assay_Malus domestica_MdAS2R3",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Malus domestica","Treatment":"5ug"},
"Additional sample data":{"Batch":"MdAS2R3","RAW_FILE_NAME":"MdAS2_R3.mzML"}
},
{
"Subject ID":"Enzyme assay_Pyrus communis_PcASR1",
"Sample ID":"Enzyme assay_Pyrus communis_PcASR1",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Pyrus communis","Treatment":"5ug"},
"Additional sample data":{"Batch":"PcASR1","RAW_FILE_NAME":"PcAS_R1.mzML"}
},
{
"Subject ID":"Enzyme assay_Pyrus communis_PcASR2",
"Sample ID":"Enzyme assay_Pyrus communis_PcASR2",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Pyrus communis","Treatment":"5ug"},
"Additional sample data":{"Batch":"PcASR2","RAW_FILE_NAME":"PcAS_R2.mzML"}
},
{
"Subject ID":"Enzyme assay_Pyrus communis_PcASR3",
"Sample ID":"Enzyme assay_Pyrus communis_PcASR3",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Pyrus communis","Treatment":"5ug"},
"Additional sample data":{"Batch":"PcASR3","RAW_FILE_NAME":"PcAS_R3.mzML"}
},
{
"Subject ID":"Enzyme assay_Apple pear hybrid_HybASR1",
"Sample ID":"Enzyme assay_Apple pear hybrid_HybASR1",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Apple pear hybrid","Treatment":"5ug"},
"Additional sample data":{"Batch":"HybASR1","RAW_FILE_NAME":"HybAS_R1.mzML"}
},
{
"Subject ID":"Enzyme assay_Apple pear hybrid_HybASR2",
"Sample ID":"Enzyme assay_Apple pear hybrid_HybASR2",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Apple pear hybrid","Treatment":"5ug"},
"Additional sample data":{"Batch":"HybASR2","RAW_FILE_NAME":"HybAS_R2.mzML"}
},
{
"Subject ID":"Enzyme assay_Apple pear hybrid_HybASR3",
"Sample ID":"Enzyme assay_Apple pear hybrid_HybASR3",
"Factors":{"Sample source":"Enzyme assay","Genotype":"Apple pear hybrid","Treatment":"5ug"},
"Additional sample data":{"Batch":"HybASR3","RAW_FILE_NAME":"HybAS_R3.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"For metabolite profiling, ripe fruit and young and old leaves of apple, pear and hybrid were collected from each individual maintained in the germplasm collection of Fondazione Edmund Mach. 100 mg of fresh tissue (FW) was extracted in 4 mL 80% v·v-1 methanol, sonicated for 20 min at 60 Hz in a water bath at 25ºC, agitated for further 20 min and kept in dark for 48 h, filtered through a 0.22 µm PTFE filter and stored at 4 ºC. For enzyme assays, E. coli strains harbouring pGEX-4T-1 with putative AS were grown in Terrific Broth (12 g·L-1 tryptone, 24 g·L-1 yeast extract, 9.4 g·L-1 K2HPO4, 2.2 g·L-1 KH2PO4, 4 mL·L-1 glycerol) at 37 °C and recombinant proteins were induced by supplementation of 0.5 mM IPTG at optical density OD600 of 0.5 – 0.6 and incubation at 20 ºC with agitation at 200 rpm for 16 h. Protein extraction was carried out by resuspending cells with B-PER™ Complete reagent supplemented with cOmplete™ protease inhibitor cocktail (Roche) followed by protein purification by Pierce™ GST spin purification kit, according to manufacter’s instructions. Quantitation of proteins was carried out by Pierce™ BCA protein assay kit and Bradford reagent (Sigma) after crude extraction and Glutathione S-Transferase (GST) - fusion protein purification, respectively. Enzyme activity was assayed in 200 µL reactions using 1 mM hydroquinone, 2 mM UDP-glucose, 5 µg purified protein in 200 mM Tris HCl, pH 7.5 buffer, incubated at 50 °C for 1 h and terminated by adding 300 µL methanol, as previously described35.","SAMPLE_TYPE":"Plant tissue/Enzyme assay","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"All samples were wildtype genotypes from Malus domestica, Pyrus communis and apple-pear hybrid, each in three replicates grown in the germplasm collection at Fondazione Edmund Mach, Italy. For recombinant protein assay, each protein was supplemented with the putative hydroquinone substrate."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"For phenolic targeted profiling, 100 mg of fresh tissue (FW) was extracted in 4 mL 80% v·v-1 methanol, sonicated for 20 min at 60 Hz in a water bath at 25ºC, agitated for further 20 min and kept in dark for 48 h, filtered through a 0.22 µm PTFE filter and stored at 4 ºC. For recombinant protein assays, each 200 µL reaction was extracted with 300 µL methanol for injection."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Ultraperformance liquid chromatography was performed on a Waters Acquity UPLC system (Milford, MA) consisting of a binary pump, an online vacuum degasser, an autosampler, and a column compartment. Separation of the phenolic compounds was achieved on a Waters Acquity HSS T3 column 1.8 μm, 100 mm × 2.1 mm (Milford, MA, USA), kept at 40 °C. Mobile phase A was water containing 0.1% formic acid; mobile phase B was acetonitrile containing 0.1% formic acid. The flow was 0.4 mL/min, and the gradient profile was 0-0.1 min, 5% B; from 0 to 3 min, linear gradient to 20% B; from 3 to 4.3 min, isocratic 20% B; from 4.3 to 9 min, linear gradient to 45% B; from 9 to 11 min, linear gradient to 100% B; from 11 to 13 min, wash at 100% B; from 13.01 to 15 min, back to the initial conditions of 5% B. The injection volume of both the standard solutions and the samples was 2 μL. After each injection, the needle was rinsed with 600 μL of weak wash solution (water/methanol, 90:10) and 200 μL of strong wash solution (methanol/water, 90:10). Samples were kept at 6 °C during the analysis.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)","SOLVENT_A":"99.9% water/0.1% formic acid","SOLVENT_B":"99.9% acetonitrile/0.1% formic acid","FLOW_GRADIENT":"0-0.1 min: 5% B, 0.1-3.0 min: linear 20%B, 3.0-4.3 min: isocratic 20% B, 4.3-9.0 min: linear 45% B, 9.0-11.0 min: linear 100% B, 11.0-13.0 min: wash 100% B, 13.01 – 15.0 min: back to initial 5% B","FLOW_RATE":"0.4 ml/min","COLUMN_TEMPERATURE":"40"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Waters Xevo TQ-XS","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Capillary voltage was 3.5 kV in positive mode and −2.5 kV in negative mode; the source was kept at 150 °C; desolvation temperature was 500 °C; cone gas flow, 50 L/h; and desolvation gas flow, 800 L/h. Unit resolution was applied to each quadrupole. Flow injections of each individual metabolite were used to optimize the MRM conditions. For the majority of the metabolites, this was done automatically by the Waters Intellistart software, whereas for some compounds the optimal cone voltages and collision energies were identified during collision-induced dissociation (CID) experiments and manually set. A dwell time of at least 25 ms was applied to each MRM transition."},

"MS_METABOLITE_DATA":{
"Units":"mg/L",

"Data":[{"Metabolite":"Quercetin","Young Leaf_Malus domestica_YLR1":"0.60","Young Leaf_Malus domestica_YLR2":"0.00","Young Leaf_Malus domestica_YLR3":"0.44","Old Leaf_Malus domestica_OLR1":"0.00","Old Leaf_Malus domestica_OLR2":"0.00","Old Leaf_Malus domestica_OLR3":"0.01","Fruit_Malus domestica_FR1":"0.00","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"0.00","Young Leaf_Pyrus communis_YLR2":"0.01","Young Leaf_Pyrus communis_YLR3":"0.03","Old Leaf_Pyrus communis_OLR1":"0.00","Old Leaf_Pyrus communis_OLR2":"0.00","Old Leaf_Pyrus communis_OLR3":"0.00","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.03","Young Leaf_Apple pear hybrid_YLR2":"0.03","Young Leaf_Apple pear hybrid_YLR3":"0.03","Old Leaf_Apple pear hybrid_OLR1":"0.00","Old Leaf_Apple pear hybrid_OLR2":"0.00","Old Leaf_Apple pear hybrid_OLR3":"0.01","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.00","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Quercetin-3''O-rhamnoside","Young Leaf_Malus domestica_YLR1":"2.35","Young Leaf_Malus domestica_YLR2":"2.31","Young Leaf_Malus domestica_YLR3":"2.22","Old Leaf_Malus domestica_OLR1":"2.34","Old Leaf_Malus domestica_OLR2":"2.48","Old Leaf_Malus domestica_OLR3":"2.73","Fruit_Malus domestica_FR1":"0.01","Fruit_Malus domestica_FR2":"0.01","Fruit_Malus domestica_FR3":"0.01","Young Leaf_Pyrus communis_YLR1":"0.04","Young Leaf_Pyrus communis_YLR2":"0.03","Young Leaf_Pyrus communis_YLR3":"0.05","Old Leaf_Pyrus communis_OLR1":"0.02","Old Leaf_Pyrus communis_OLR2":"0.04","Old Leaf_Pyrus communis_OLR3":"0.04","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.24","Young Leaf_Apple pear hybrid_YLR2":"0.24","Young Leaf_Apple pear hybrid_YLR3":"0.28","Old Leaf_Apple pear hybrid_OLR1":"0.44","Old Leaf_Apple pear hybrid_OLR2":"0.41","Old Leaf_Apple pear hybrid_OLR3":"0.47","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.00","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Quercetin-3''O-glucoside","Young Leaf_Malus domestica_YLR1":"1.69","Young Leaf_Malus domestica_YLR2":"1.72","Young Leaf_Malus domestica_YLR3":"1.62","Old Leaf_Malus domestica_OLR1":"1.38","Old Leaf_Malus domestica_OLR2":"1.54","Old Leaf_Malus domestica_OLR3":"1.52","Fruit_Malus domestica_FR1":"0.01","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"0.09","Young Leaf_Pyrus communis_YLR2":"0.09","Young Leaf_Pyrus communis_YLR3":"0.08","Old Leaf_Pyrus communis_OLR1":"0.27","Old Leaf_Pyrus communis_OLR2":"0.26","Old Leaf_Pyrus communis_OLR3":"0.22","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.13","Young Leaf_Apple pear hybrid_YLR2":"0.13","Young Leaf_Apple pear hybrid_YLR3":"0.17","Old Leaf_Apple pear hybrid_OLR1":"0.61","Old Leaf_Apple pear hybrid_OLR2":"0.68","Old Leaf_Apple pear hybrid_OLR3":"0.61","Fruit_Apple pear hybrid_FR1":"0.01","Fruit_Apple pear hybrid_FR2":"0.01","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Quercetin-3''O-galactoside","Young Leaf_Malus domestica_YLR1":"1.69","Young Leaf_Malus domestica_YLR2":"1.72","Young Leaf_Malus domestica_YLR3":"1.62","Old Leaf_Malus domestica_OLR1":"1.38","Old Leaf_Malus domestica_OLR2":"1.54","Old Leaf_Malus domestica_OLR3":"1.52","Fruit_Malus domestica_FR1":"0.01","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"0.65","Young Leaf_Pyrus communis_YLR2":"0.60","Young Leaf_Pyrus communis_YLR3":"0.67","Old Leaf_Pyrus communis_OLR1":"0.61","Old Leaf_Pyrus communis_OLR2":"0.54","Old Leaf_Pyrus communis_OLR3":"0.54","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.13","Young Leaf_Apple pear hybrid_YLR2":"0.13","Young Leaf_Apple pear hybrid_YLR3":"0.17","Old Leaf_Apple pear hybrid_OLR1":"0.43","Old Leaf_Apple pear hybrid_OLR2":"0.31","Old Leaf_Apple pear hybrid_OLR3":"0.61","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.01","Fruit_Apple pear hybrid_FR3":"0.01","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Rutin","Young Leaf_Malus domestica_YLR1":"0.00","Young Leaf_Malus domestica_YLR2":"0.00","Young Leaf_Malus domestica_YLR3":"0.00","Old Leaf_Malus domestica_OLR1":"0.28","Old Leaf_Malus domestica_OLR2":"0.26","Old Leaf_Malus domestica_OLR3":"0.35","Fruit_Malus domestica_FR1":"0.00","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"13.89","Young Leaf_Pyrus communis_YLR2":"14.12","Young Leaf_Pyrus communis_YLR3":"15.52","Old Leaf_Pyrus communis_OLR1":"11.20","Old Leaf_Pyrus communis_OLR2":"11.25","Old Leaf_Pyrus communis_OLR3":"11.50","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"10.95","Young Leaf_Apple pear hybrid_YLR2":"11.87","Young Leaf_Apple pear hybrid_YLR3":"12.42","Old Leaf_Apple pear hybrid_OLR1":"5.86","Old Leaf_Apple pear hybrid_OLR2":"6.67","Old Leaf_Apple pear hybrid_OLR3":"6.73","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.01","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Kaempferol-3''O-glucoside","Young Leaf_Malus domestica_YLR1":"0.13","Young Leaf_Malus domestica_YLR2":"0.14","Young Leaf_Malus domestica_YLR3":"0.12","Old Leaf_Malus domestica_OLR1":"0.05","Old Leaf_Malus domestica_OLR2":"0.04","Old Leaf_Malus domestica_OLR3":"0.05","Fruit_Malus domestica_FR1":"0.00","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"0.16","Young Leaf_Pyrus communis_YLR2":"0.16","Young Leaf_Pyrus communis_YLR3":"0.16","Old Leaf_Pyrus communis_OLR1":"0.06","Old Leaf_Pyrus communis_OLR2":"0.07","Old Leaf_Pyrus communis_OLR3":"0.08","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.26","Young Leaf_Apple pear hybrid_YLR2":"0.26","Young Leaf_Apple pear hybrid_YLR3":"0.28","Old Leaf_Apple pear hybrid_OLR1":"0.15","Old Leaf_Apple pear hybrid_OLR2":"0.16","Old Leaf_Apple pear hybrid_OLR3":"0.17","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.00","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Kaempferol-3''O-rutinoside","Young Leaf_Malus domestica_YLR1":"0.37","Young Leaf_Malus domestica_YLR2":"0.44","Young Leaf_Malus domestica_YLR3":"0.39","Old Leaf_Malus domestica_OLR1":"0.00","Old Leaf_Malus domestica_OLR2":"0.00","Old Leaf_Malus domestica_OLR3":"0.00","Fruit_Malus domestica_FR1":"0.00","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"1.45","Young Leaf_Pyrus communis_YLR2":"1.45","Young Leaf_Pyrus communis_YLR3":"1.79","Old Leaf_Pyrus communis_OLR1":"0.69","Old Leaf_Pyrus communis_OLR2":"0.61","Old Leaf_Pyrus communis_OLR3":"0.74","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"2.14","Young Leaf_Apple pear hybrid_YLR2":"2.25","Young Leaf_Apple pear hybrid_YLR3":"2.39","Old Leaf_Apple pear hybrid_OLR1":"0.58","Old Leaf_Apple pear hybrid_OLR2":"0.74","Old Leaf_Apple pear hybrid_OLR3":"0.67","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.00","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Laricitrin","Young Leaf_Malus domestica_YLR1":"0.05","Young Leaf_Malus domestica_YLR2":"0.00","Young Leaf_Malus domestica_YLR3":"0.04","Old Leaf_Malus domestica_OLR1":"0.05","Old Leaf_Malus domestica_OLR2":"0.05","Old Leaf_Malus domestica_OLR3":"0.04","Fruit_Malus domestica_FR1":"0.01","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"0.05","Young Leaf_Pyrus communis_YLR2":"0.00","Young Leaf_Pyrus communis_YLR3":"0.05","Old Leaf_Pyrus communis_OLR1":"0.05","Old Leaf_Pyrus communis_OLR2":"0.00","Old Leaf_Pyrus communis_OLR3":"0.04","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.01","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.04","Young Leaf_Apple pear hybrid_YLR2":"0.05","Young Leaf_Apple pear hybrid_YLR3":"0.00","Old Leaf_Apple pear hybrid_OLR1":"0.05","Old Leaf_Apple pear hybrid_OLR2":"0.04","Old Leaf_Apple pear hybrid_OLR3":"0.05","Fruit_Apple pear hybrid_FR1":"0.01","Fruit_Apple pear hybrid_FR2":"0.01","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Isorhamnetin-3''O-glucoside","Young Leaf_Malus domestica_YLR1":"0.01","Young Leaf_Malus domestica_YLR2":"0.02","Young Leaf_Malus domestica_YLR3":"0.01","Old Leaf_Malus domestica_OLR1":"0.00","Old Leaf_Malus domestica_OLR2":"0.00","Old Leaf_Malus domestica_OLR3":"0.00","Fruit_Malus domestica_FR1":"0.00","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"0.03","Young Leaf_Pyrus communis_YLR2":"0.03","Young Leaf_Pyrus communis_YLR3":"0.05","Old Leaf_Pyrus communis_OLR1":"0.06","Old Leaf_Pyrus communis_OLR2":"0.09","Old Leaf_Pyrus communis_OLR3":"0.09","Fruit_Pyrus communis_FR1":"0.01","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.00","Young Leaf_Apple pear hybrid_YLR2":"0.01","Young Leaf_Apple pear hybrid_YLR3":"0.00","Old Leaf_Apple pear hybrid_OLR1":"0.00","Old Leaf_Apple pear hybrid_OLR2":"0.00","Old Leaf_Apple pear hybrid_OLR3":"0.01","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.00","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Isorhamnetin-3''O-rutinoside","Young Leaf_Malus domestica_YLR1":"0.04","Young Leaf_Malus domestica_YLR2":"0.05","Young Leaf_Malus domestica_YLR3":"0.05","Old Leaf_Malus domestica_OLR1":"0.01","Old Leaf_Malus domestica_OLR2":"0.01","Old Leaf_Malus domestica_OLR3":"0.01","Fruit_Malus domestica_FR1":"0.00","Fruit_Malus domestica_FR2":"0.00","Fruit_Malus domestica_FR3":"0.00","Young Leaf_Pyrus communis_YLR1":"0.94","Young Leaf_Pyrus communis_YLR2":"0.87","Young Leaf_Pyrus communis_YLR3":"1.04","Old Leaf_Pyrus communis_OLR1":"0.65","Old Leaf_Pyrus communis_OLR2":"0.57","Old Leaf_Pyrus communis_OLR3":"0.60","Fruit_Pyrus communis_FR1":"0.00","Fruit_Pyrus communis_FR2":"0.00","Fruit_Pyrus communis_FR3":"0.00","Young Leaf_Apple pear hybrid_YLR1":"0.54","Young Leaf_Apple pear hybrid_YLR2":"0.55","Young Leaf_Apple pear hybrid_YLR3":"0.63","Old Leaf_Apple pear hybrid_OLR1":"0.15","Old Leaf_Apple pear hybrid_OLR2":"0.19","Old Leaf_Apple pear hybrid_OLR3":"0.20","Fruit_Apple pear hybrid_FR1":"0.00","Fruit_Apple pear hybrid_FR2":"0.00","Fruit_Apple pear hybrid_FR3":"0.00","Enzyme assay_Malus domestica_MdAS1R1":"0.00","Enzyme assay_Malus domestica_MdAS1R2":"0.00","Enzyme assay_Malus domestica_MdAS1R3":"0.00","Enzyme assay_Malus domestica_MdAS2R1":"0.00","Enzyme assay_Malus domestica_MdAS2R2":"0.00","Enzyme assay_Malus domestica_MdAS2R3":"0.00","Enzyme assay_Pyrus communis_PcASR1":"0.00","Enzyme assay_Pyrus communis_PcASR2":"0.00","Enzyme assay_Pyrus communis_PcASR3":"0.00","Enzyme assay_Apple pear hybrid_HybASR1":"0.00","Enzyme assay_Apple pear hybrid_HybASR2":"0.00","Enzyme assay_Apple pear hybrid_HybASR3":"0.00"},{"Metabolite":"Hydroquinone","Young Leaf_Malus domestica_YLR1":"0.2","Young Leaf_Malus domestica_YLR2":"0.0","Young Leaf_Malus domestica_YLR3":"0.5","Old Leaf_Malus domestica_OLR1":"0.0","Old Leaf_Malus domestica_OLR2":"0.6","Old Leaf_Malus domestica_OLR3":"6.3","Fruit_Malus domestica_FR1":"0.0","Fruit_Malus domestica_FR2":"0.0","Fruit_Malus domestica_FR3":"0.0","Young Leaf_Pyrus communis_YLR1":"30.2","Young Leaf_Pyrus communis_YLR2":"29.2","Young Leaf_Pyrus communis_YLR3":"27.2","Old Leaf_Pyrus communis_OLR1":"7.8","Old Leaf_Pyrus communis_OLR2":"6.2","Old Leaf_Pyrus communis_OLR3":"5.9","Fruit_Pyrus communis_FR1":"0.0","Fruit_Pyrus communis_FR2":"0.0","Fruit_Pyrus communis_FR3":"0.0","Young Leaf_Apple pear hybrid_YLR1":"14.7","Young Leaf_Apple pear hybrid_YLR2":"14.3","Young Leaf_Apple pear hybrid_YLR3":"12.5","Old Leaf_Apple pear hybrid_OLR1":"10.3","Old Leaf_Apple pear hybrid_OLR2":"11.3","Old Leaf_Apple pear hybrid_OLR3":"10.9","Fruit_Apple pear hybrid_FR1":"0.0","Fruit_Apple pear hybrid_FR2":"0.0","Fruit_Apple pear hybrid_FR3":"0.1","Enzyme assay_Malus domestica_MdAS1R1":"19.76","Enzyme assay_Malus domestica_MdAS1R2":"20.04","Enzyme assay_Malus domestica_MdAS1R3":"18.3","Enzyme assay_Malus domestica_MdAS2R1":"26.09","Enzyme assay_Malus domestica_MdAS2R2":"24.12","Enzyme assay_Malus domestica_MdAS2R3":"26.66","Enzyme assay_Pyrus communis_PcASR1":"24.21","Enzyme assay_Pyrus communis_PcASR2":"18.79","Enzyme assay_Pyrus communis_PcASR3":"22.03","Enzyme assay_Apple pear hybrid_HybASR1":"19.69","Enzyme assay_Apple pear hybrid_HybASR2":"21.24","Enzyme assay_Apple pear hybrid_HybASR3":"12.72"}],

"Metabolites":[{"metabolite_name":"Quercetin"},{"metabolite_name":"Quercetin-3''O-rhamnoside"},{"metabolite_name":"Quercetin-3''O-glucoside"},{"metabolite_name":"Quercetin-3''O-galactoside"},{"metabolite_name":"Rutin"},{"metabolite_name":"Kaempferol-3''O-glucoside"},{"metabolite_name":"Kaempferol-3''O-rutinoside"},{"metabolite_name":"Laricitrin"},{"metabolite_name":"Isorhamnetin-3''O-glucoside"},{"metabolite_name":"Isorhamnetin-3''O-rutinoside"},{"metabolite_name":"Hydroquinone"}]
}

}