{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003186","ANALYSIS_ID":"AN005234","VERSION":"1","CREATED_ON":"April 29, 2024, 12:00 pm"}, "PROJECT":{"PROJECT_TITLE":"A metabolomics pipeline highlights microbial metabolism in bloodstream infections - untargeted metabolomics on mouse model of cecal slurry (CS)","PROJECT_SUMMARY":"untargeted metabolomics on plasma from male B6 mice 24hrs after IP injection of PBS, heat killed cecal slurry, or live cecal slurry","INSTITUTE":"Harvard University","LAST_NAME":"Mayers","FIRST_NAME":"Jared","ADDRESS":"12 Oxford St Conant 200S, Cambridge, MA, 02138, USA","EMAIL":"jrmayers@gmail.com","PHONE":"4259417747"}, "STUDY":{"STUDY_TITLE":"CS Large Group","STUDY_SUMMARY":"untargeted metabolomics on plasma from male B6 mice 24hrs after IP injection of PBS, heat killed cecal slurry, or live cecal slurry","INSTITUTE":"Harvard University","LAST_NAME":"Mayers","FIRST_NAME":"Jared","ADDRESS":"12 Oxford St Conant 200S Cambridge, MA 02138","EMAIL":"jrmayers@gmail.com","PHONE":"4259417747"}, "SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"24hr_1", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_1"} }, { "Subject ID":"-", "Sample ID":"24hr_2", "Factors":{"Factor":"Heat Killed","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_2"} }, { "Subject ID":"-", "Sample ID":"24hr_3", "Factors":{"Factor":"Live","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_3"} }, { "Subject ID":"-", "Sample ID":"24hr_4", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_4"} }, { "Subject ID":"-", "Sample ID":"24hr_5", "Factors":{"Factor":"Heat Killed","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_5"} }, { "Subject ID":"-", "Sample ID":"24hr_6", "Factors":{"Factor":"Live","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_6"} }, { "Subject ID":"-", "Sample ID":"24hr_7", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_7"} }, { "Subject ID":"-", "Sample ID":"24hr_8", "Factors":{"Factor":"Heat Killed","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_8"} }, { "Subject ID":"-", "Sample ID":"24hr_10", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_10"} }, { "Subject ID":"-", "Sample ID":"24hr_11", "Factors":{"Factor":"Heat Killed","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_11"} }, { "Subject ID":"-", "Sample ID":"24hr_12", "Factors":{"Factor":"Live","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_12"} }, { "Subject ID":"-", "Sample ID":"24hr_13", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_13"} }, { "Subject ID":"-", "Sample ID":"24hr_14", "Factors":{"Factor":"Heat Killed","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_14"} }, { "Subject ID":"-", "Sample ID":"24hr_16", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_16"} }, { "Subject ID":"-", "Sample ID":"24hr_17", "Factors":{"Factor":"Heat Killed","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_17"} }, { "Subject ID":"-", "Sample ID":"24hr_19", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_19"} }, { "Subject ID":"-", "Sample ID":"24hr_22", "Factors":{"Factor":"Control","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_22"} }, { "Subject ID":"-", "Sample ID":"24hr_23", "Factors":{"Factor":"Heat Killed","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_23"} }, { "Subject ID":"-", "Sample ID":"24hr_24", "Factors":{"Factor":"Live","Sample source":"Plasma"}, "Additional sample data":{"RAW_FILE_NAME(File Name)":"24hr_24"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Plasma samples from terminal bleed via cardiac puncture under anesthesia","SAMPLE_TYPE":"Blood (plasma)"}, "TREATMENT":{"TREATMENT_SUMMARY":"IP injection with either PBS, heat killed cecal slurry or live cecal slurry 24hrs prior to blood draw"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"1 part plasma with 9 parts 75:25:0.2 ACN:MetOH:Formic Acid with internal val-d8 and phe-d8 standards; vortexed, centrifuged, supernatant removed and run on LC-MS"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent 1260 Infinity HPLC","COLUMN_NAME":"Waters Atlantis HILIC (150 x 2.1mm,3um)","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100% acetonitrile; 0.1% formic acid","FLOW_GRADIENT":"0.5 minute at 5% mobile phase B followed by a linear gradient to 40% mobile phase B over 10 minutes","FLOW_RATE":"250 μL/min","COLUMN_TEMPERATURE":"30"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Agilent 6530 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Raw data from the LC–MS were analyzed using Agilent MassHunter Qualitative Analysis 10.0 software.","MS_RESULTS_FILE":"ST003186_AN005234_Results.txt UNITS:ion counts Has m/z:Yes Has RT:Yes RT units:Minutes"} }