{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003190","ANALYSIS_ID":"AN005238","PROJECT_ID":"PR001986","VERSION":"1","CREATED_ON":"May 6, 2024, 7:26 pm"},

"PROJECT":{"PROJECT_TITLE":"A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate the inositol hexaphosphate accumulation","PROJECT_TYPE":"research","PROJECT_SUMMARY":"Inositol hexaphosphate (InsP6) is the major storage form of phosphorus in seeds. Reducing seed InsP6 content is a breeding objective in agriculture, as InsP6 negatively impacts animal nutrition and the environment. Nevertheless, how InsP6 accumulation is regulated remains largely unknown. Here, we identify a clade of receptor-like cytoplasmic kinases (RLCKs), named Inositol Polyphosphate-related Cytoplasmic Kinases 1-6 (IPCK1-IPCK6), deeply involved in InsP6 accumulation. The InsP6 concentration is dramatically reduced in seeds of ipck quadruple (T-4m/C-4m) and quintuple (C-5m) mutants, accompanied with the obviously increase of phosphate (Pi) concentration. The plasma membrane-localized IPCKs recruit IPK1 involved in InsP6 synthesis, and facilitate its binding and activity via phosphorylation of GRF 14-3-3 proteins. IPCKs also recruit IPK2s and PI-PLCs required for InsP4/InsP5 and InsP3 biosynthesis respectively, to form a potential IPCK-GRF-PLC-IPK2-IPK1 complex. Our findings therefore uncover a previously uncharacterized regulatory mechanism of InsP6 accumulation governed by IPCKs, shedding new light on the mechanisms of InsP biosynthesis in eukaryotes.","INSTITUTE":"zhejiang University","LAST_NAME":"XU","FIRST_NAME":"LILIN","ADDRESS":"866 yuhangtang road, hangzhou, zhejiang, 310027, China","EMAIL":"1164702127@qq.com","PHONE":"18667919279"},

"STUDY":{"STUDY_TITLE":"A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate the inositol hexaphosphate accumulation","STUDY_SUMMARY":"Inositol hexaphosphate (InsP6) is the major storage form of phosphorus in seeds. Reducing seed InsP6 content is a breeding objective in agriculture, as InsP6 negatively impacts animal nutrition and the environment. Nevertheless, how InsP6 accumulation is regulated remains largely unknown. Here, we identify a clade of receptor-like cytoplasmic kinases (RLCKs), named Inositol Polyphosphate-related Cytoplasmic Kinases 1-6 (IPCK1-IPCK6), deeply involved in InsP6 accumulation. The InsP6 concentration is dramatically reduced in seeds of ipck quadruple (T-4m/C-4m) and quintuple (C-5m) mutants, accompanied with the obviously increase of phosphate (Pi) concentration. The plasma membrane-localized IPCKs recruit IPK1 involved in InsP6 synthesis, and facilitate its binding and activity via phosphorylation of GRF 14-3-3 proteins. IPCKs also recruit IPK2s and PI-PLCs required for InsP4/InsP5 and InsP3 biosynthesis respectively, to form a potential IPCK-GRF-PLC-IPK2-IPK1 complex. Our findings therefore uncover a previously uncharacterized regulatory mechanism of InsP6 accumulation governed by IPCKs, shedding new light on the mechanisms of InsP biosynthesis in eukaryotes.","INSTITUTE":"Zhejiang University","LAST_NAME":"XU","FIRST_NAME":"LILIN","ADDRESS":"zhejiang university","EMAIL":"1164702127@qq.com","PHONE":"18667919279"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Arabidopsis thaliana","TAXONOMY_ID":"3702"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"WT-IP3-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP3-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-IP4-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP4-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-IP5-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP5-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-IP6-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP6-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-IP7-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP7-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-IP8-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP8-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-IP5-Fig2f",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP5-Fig2f.xml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-IP6-Fig2f",
"Factors":{"Sample source":"Plant Seed","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"WT-IP6-Fig2f.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP3-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP3-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP4-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP4-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP5-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP5-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP6-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP6-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP7-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP7-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP8-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP8-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP5-Fig2f",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP5-Fig2f.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-1-IP6-Fig2f",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-1-IP6-Fig2f.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP3-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP3-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP4-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP4-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP5-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP5-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP6-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP6-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP7-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP7-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP8-FigS2b",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP8-FigS2b.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP5-Fig2f",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP5-Fig2f.xml"}
},
{
"Subject ID":"-",
"Sample ID":"C-5m-2-IP6-Fig2f",
"Factors":{"Sample source":"Plant Seed","Treatment":"mutant"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"C-5m-2-IP6-Fig2f.xml"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"1 g total seedlings grown on agar medium for 15 d or 0.1 g dry seeds were collected for InsP5/InsP6 detection; For InsP3/InsP4/InsP5/InsP6/InsP7/InsP8 detection in seeds and seedlings, 10 g of 12-day-old seedlings or 2.4 g of dry seeds were used for InsPs enrichment with 300 mg of TiO2 beads for each sample.","SAMPLE_TYPE":"Plant"},

"TREATMENT":{"TREATMENT_SUMMARY":"No treatment, All seedlings are planted on 1/2 MS plates, and the seeds are healthy and dry."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The enrichment of InsPs with TiO2 beads and SDS-PAGE assay were performed as described by Wilson et al47. In brief, 1 g total seedlings grown on agar medium for 15 d or 0.1 g dry seeds were ground in liquid nitrogen, suspended in 5 ml of 1 M cold perchloric acid, and kept rotating for 15 min at 4 ℃. After centrifugation at 12,000 g for 10 min at 4 ℃, the supernatant was transferred into a new tube containing 30 mg of TiO2 beads (5 mm Titansphere; GL Sciences, Japan) and kept rotating at 4 ℃ for 20 - 30 min. After centrifugation at 5000 g for 10 min at 4 ℃, the beads were transferred into a new 1.5 ml tube and washed with pre-cold PA for 3-5 times, then eluted with 500 µl of 10 % ammonia solution. The eluate was freeze-dried at -50 ℃ and resuspended with 50 µl of 10 % ammonia solution, and the enriched InsPs was resolved in a 33 % polyacrylamide / Tris–borate–EDTA (TBE) gel and stained with toluidine blue. 10 µM synthetic InsP5 (myo-Inositol-1,3,4,5,6-pentaphosphate ammonium salt, Cayman) and InsP6 (Sichem) were used as the markers and standards. In order to verify the specific components in the eluate, the total eluate was firstly freeze-dried, then dissolved with 100 µl 80 % acetonitrile."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"InsPs were detected using Hydrophilic Interaction High Performance Liquid Chromatography-Tandem Mass Spectrometry on an Agilent 1290 infinity HPLC system coupled to an Agilent 6460 triple Quad LC-MS/MS using InfinityLab Poroshell 120 HILIC-Z (2.1 × 100) column (Agilent Technologies, USA). Nitrogen was used as the sheath gas and drying gas. The nebulizer pressure was set to 45 psi and the flow rate of drying gas was 5 L / min. The flow rate and temperature of the sheath gas were 11 L / min and 350℃, respectively. Chromatographic separation was carried out on a HPLC column (100 × 2.1 mm, 2.7 µm). The column temperature was 35℃. The mobile phases consisted of (A) ammonium acetate in distilled water (pH 10.0) and (B) Acetonitrile. The gradient program was as follows: 0-10 min, 90 % → 55 % of B; 10-12 min, 55 % → 90 % of B; 12-20 min, 90 % of B. The flow rate was set at 0.3 ml / min, and the injection volume was 10 μl. Mass spectrometric detection was completed by use of an electrospray ionization (ESI) source in negative ion multiple-reaction monitoring (MRM) mode. InsPs were identified based on comparison to known InsPs species. The mass spectrometry parameters corresponding to different InsPs show as below: InsP3 (MRM: 419 -> 321, 419 -> 337, Acquisition time is 6-7 min); InsP4 (MRM: 499 -> 401, 499 -> 417, Acquisition time is 6-7 min); InsP5 (MRM: 579 -> 480.9, 579 -> 382.8, Acquisition time is 6-7 min); InsP6 (MRM: 659 -> 560.8, 659 -> 577, Acquisition time is 6-7 min); InsP7 (MRM: 739 -> 575, 739 -> 657, Acquisition time is 5-6 min); InsP8 (MRM: 819 -> 737, 819 -> 655, Acquisition time is 4-5 min). According to the regression equation calculated from the standard sample, substitute the response value of the sample into the equation to convert the corresponding concentration. For InsP3/InsP4/InsP5/InsP6/InsP7/InsP8 detection in seeds and seedlings, 10 g of 12-day-old seedlings or 2.4 g of dry seeds were used for InsPs enrichment with 300 mg of TiO2 beads for each sample. The enriched substances were analyzed by HPLC-MS/MS. The purchased InsP3 (1,4,5-InsP3, MedChemExpress), InsP4 (1,3,4,5-InsP4, MedChemExpress), InsP5 and InsP6, InsP7 (5-InsP7) and InsP8 (1,5-InsP8) from Lei lab25 were used as standard samples for generating the calibration curves.","CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent 1290 Infinity","COLUMN_NAME":"Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)","SOLVENT_A":"100% Distilled Water; 10% ammonium acetate (pH 10.0)","SOLVENT_B":"100% Acetonitrile","FLOW_GRADIENT":"0-10 min, 90 % → 55 % of B; 10-12 min, 55 % → 90 % of B; 12-20 min, 90 % of B","FLOW_RATE":"0.3 mL/min","COLUMN_TEMPERATURE":"35℃"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 5973","INSTRUMENT_TYPE":"HPLC-MS/MS","MS_TYPE":"Other","ION_MODE":"POSITIVE","MS_COMMENTS":"InsPs were detected using Hydrophilic Interaction High Performance Liquid Chromatography-Tandem Mass Spectrometry on an Agilent 1290 infinity HPLC system coupled to an Agilent 6460 triple Quad LC-MS/MS using InfinityLab Poroshell 120 HILIC-Z (2.1 × 100) column (Agilent Technologies, USA). Nitrogen was used as the sheath gas and drying gas. The nebulizer pressure was set to 45 psi and the flow rate of drying gas was 5 L / min. The flow rate and temperature of the sheath gas were 11 L / min and 350℃, respectively. Chromatographic separation was carried out on a HPLC column (100 × 2.1 mm, 2.7 µm). The column temperature was 35℃. The mobile phases consisted of (A) ammonium acetate in distilled water (pH 10.0) and (B) Acetonitrile. The gradient program was as follows: 0-10 min, 90 % → 55 % of B; 10-12 min, 55 % → 90 % of B; 12-20 min, 90 % of B. The flow rate was set at 0.3 ml / min, and the injection volume was 10 μl. Mass spectrometric detection was completed by use of an electrospray ionization (ESI) source in negative ion multiple-reaction monitoring (MRM) mode. InsPs were identified based on comparison to known InsPs species. The mass spectrometry parameters corresponding to different InsPs show as below: InsP3 (MRM: 419 -> 321, 419 -> 337, Acquisition time is 6-7 min); InsP4 (MRM: 499 -> 401, 499 -> 417, Acquisition time is 6-7 min); InsP5 (MRM: 579 -> 480.9, 579 -> 382.8, Acquisition time is 6-7 min); InsP6 (MRM: 659 -> 560.8, 659 -> 577, Acquisition time is 6-7 min); InsP7 (MRM: 739 -> 575, 739 -> 657, Acquisition time is 5-6 min); InsP8 (MRM: 819 -> 737, 819 -> 655, Acquisition time is 4-5 min). According to the regression equation calculated from the standard sample, substitute the response value of the sample into the equation to convert the corresponding concentration."},

"MS_METABOLITE_DATA":{
"Units":"mg/g",

"Data":[{"Metabolite":"IP3","WT-IP3-FigS2b":"0.1855","WT-IP4-FigS2b":"","WT-IP5-FigS2b":"","WT-IP6-FigS2b":"","WT-IP7-FigS2b":"","WT-IP8-FigS2b":"","WT-IP5-Fig2f":"","WT-IP6-Fig2f":"","C-5m-1-IP3-FigS2b":"0.1424","C-5m-1-IP4-FigS2b":"","C-5m-1-IP5-FigS2b":"","C-5m-1-IP6-FigS2b":"","C-5m-1-IP7-FigS2b":"","C-5m-1-IP8-FigS2b":"","C-5m-1-IP5-Fig2f":"","C-5m-1-IP6-Fig2f":"","C-5m-2-IP3-FigS2b":"0.1527","C-5m-2-IP4-FigS2b":"","C-5m-2-IP5-FigS2b":"","C-5m-2-IP6-FigS2b":"","C-5m-2-IP7-FigS2b":"","C-5m-2-IP8-FigS2b":"","C-5m-2-IP5-Fig2f":"","C-5m-2-IP6-Fig2f":""},{"Metabolite":"IP4","WT-IP3-FigS2b":"","WT-IP4-FigS2b":"0.1657","WT-IP5-FigS2b":"","WT-IP6-FigS2b":"","WT-IP7-FigS2b":"","WT-IP8-FigS2b":"","WT-IP5-Fig2f":"","WT-IP6-Fig2f":"","C-5m-1-IP3-FigS2b":"","C-5m-1-IP4-FigS2b":"0.1081","C-5m-1-IP5-FigS2b":"","C-5m-1-IP6-FigS2b":"","C-5m-1-IP7-FigS2b":"","C-5m-1-IP8-FigS2b":"","C-5m-1-IP5-Fig2f":"","C-5m-1-IP6-Fig2f":"","C-5m-2-IP3-FigS2b":"","C-5m-2-IP4-FigS2b":"0.1252","C-5m-2-IP5-FigS2b":"","C-5m-2-IP6-FigS2b":"","C-5m-2-IP7-FigS2b":"","C-5m-2-IP8-FigS2b":"","C-5m-2-IP5-Fig2f":"","C-5m-2-IP6-Fig2f":""},{"Metabolite":"IP5","WT-IP3-FigS2b":"","WT-IP4-FigS2b":"","WT-IP5-FigS2b":"0.3576","WT-IP6-FigS2b":"","WT-IP7-FigS2b":"","WT-IP8-FigS2b":"","WT-IP5-Fig2f":"3.279806876","WT-IP6-Fig2f":"","C-5m-1-IP3-FigS2b":"","C-5m-1-IP4-FigS2b":"","C-5m-1-IP5-FigS2b":"0.1624","C-5m-1-IP6-FigS2b":"","C-5m-1-IP7-FigS2b":"","C-5m-1-IP8-FigS2b":"","C-5m-1-IP5-Fig2f":"1.04324096","C-5m-1-IP6-Fig2f":"","C-5m-2-IP3-FigS2b":"","C-5m-2-IP4-FigS2b":"","C-5m-2-IP5-FigS2b":"0.1464","C-5m-2-IP6-FigS2b":"","C-5m-2-IP7-FigS2b":"","C-5m-2-IP8-FigS2b":"","C-5m-2-IP5-Fig2f":"0.882777046","C-5m-2-IP6-Fig2f":""},{"Metabolite":"IP6","WT-IP3-FigS2b":"","WT-IP4-FigS2b":"","WT-IP5-FigS2b":"","WT-IP6-FigS2b":"3.0960","WT-IP7-FigS2b":"","WT-IP8-FigS2b":"","WT-IP5-Fig2f":"","WT-IP6-Fig2f":"0.003096","C-5m-1-IP3-FigS2b":"","C-5m-1-IP4-FigS2b":"","C-5m-1-IP5-FigS2b":"","C-5m-1-IP6-FigS2b":"1.1800","C-5m-1-IP7-FigS2b":"","C-5m-1-IP8-FigS2b":"","C-5m-1-IP5-Fig2f":"","C-5m-1-IP6-Fig2f":"0.00118","C-5m-2-IP3-FigS2b":"","C-5m-2-IP4-FigS2b":"","C-5m-2-IP5-FigS2b":"","C-5m-2-IP6-FigS2b":"1.0234","C-5m-2-IP7-FigS2b":"","C-5m-2-IP8-FigS2b":"","C-5m-2-IP5-Fig2f":"","C-5m-2-IP6-Fig2f":"0.0010234"},{"Metabolite":"IP7","WT-IP3-FigS2b":"","WT-IP4-FigS2b":"","WT-IP5-FigS2b":"","WT-IP6-FigS2b":"","WT-IP7-FigS2b":"0.0643","WT-IP8-FigS2b":"","WT-IP5-Fig2f":"","WT-IP6-Fig2f":"","C-5m-1-IP3-FigS2b":"","C-5m-1-IP4-FigS2b":"","C-5m-1-IP5-FigS2b":"","C-5m-1-IP6-FigS2b":"","C-5m-1-IP7-FigS2b":"0.0613","C-5m-1-IP8-FigS2b":"","C-5m-1-IP5-Fig2f":"","C-5m-1-IP6-Fig2f":"","C-5m-2-IP3-FigS2b":"","C-5m-2-IP4-FigS2b":"","C-5m-2-IP5-FigS2b":"","C-5m-2-IP6-FigS2b":"","C-5m-2-IP7-FigS2b":"0.0604","C-5m-2-IP8-FigS2b":"","C-5m-2-IP5-Fig2f":"","C-5m-2-IP6-Fig2f":""},{"Metabolite":"IP8","WT-IP3-FigS2b":"","WT-IP4-FigS2b":"","WT-IP5-FigS2b":"","WT-IP6-FigS2b":"","WT-IP7-FigS2b":"","WT-IP8-FigS2b":"0.1686","WT-IP5-Fig2f":"","WT-IP6-Fig2f":"","C-5m-1-IP3-FigS2b":"","C-5m-1-IP4-FigS2b":"","C-5m-1-IP5-FigS2b":"","C-5m-1-IP6-FigS2b":"","C-5m-1-IP7-FigS2b":"","C-5m-1-IP8-FigS2b":"0.0955","C-5m-1-IP5-Fig2f":"","C-5m-1-IP6-Fig2f":"","C-5m-2-IP3-FigS2b":"","C-5m-2-IP4-FigS2b":"","C-5m-2-IP5-FigS2b":"","C-5m-2-IP6-FigS2b":"","C-5m-2-IP7-FigS2b":"","C-5m-2-IP8-FigS2b":"0.0912","C-5m-2-IP5-Fig2f":"","C-5m-2-IP6-Fig2f":""}],

"Metabolites":[{"metabolite_name":"IP3"},{"metabolite_name":"IP4"},{"metabolite_name":"IP5"},{"metabolite_name":"IP6"},{"metabolite_name":"IP7"},{"metabolite_name":"IP8"}]
}

}