{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003306","ANALYSIS_ID":"AN005417","PROJECT_ID":"PR002056","VERSION":"1","CREATED_ON":"July 9, 2024, 12:26 pm"},

"PROJECT":{"PROJECT_TITLE":"ASCT2 is a major contributor to serine uptake in cancer cells","PROJECT_TYPE":"GCMS quantitative analysis","PROJECT_SUMMARY":"Polar metabolite abundance studies from MCF7 human ER+ breast cancer cell line with or without CRISPR-Cas9 knockout of ASCT2. Studies used RPMI media with either complete serine levels (285uM), low serine levels (50uM), or no glutamine.","INSTITUTE":"University of Illinois Chicago","DEPARTMENT":"Physiology and Biophysics","LABORATORY":"Coloff Lab","LAST_NAME":"Conger","FIRST_NAME":"Kelly","ADDRESS":"909 S Wolcott Ave, Chicago, IL, 60612","EMAIL":"kconge2@uic.edu","PHONE":"2314320406"},

"STUDY":{"STUDY_TITLE":"ASCT2 is a major contributor to serine uptake in cancer cells","STUDY_SUMMARY":"The non-essential amino acid serine is a critical nutrient for cancer cells due to its diverse biosynthetic functions. While some tumors can synthesize serine de novo, others are auxotrophic and therefore reliant on serine uptake. Importantly, despite several transporters being known to be capable of transporting serine, the transporter(s) that mediate serine uptake in cancer cells are not known. Here, we characterize the amino acid transporter ASCT2 (SLC1A5) as a major contributor to serine uptake in cancer cells. ASCT2 is well-known as a glutamine transporter in cancer, and our work demonstrates that serine and glutamine compete for uptake through ASCT2. We further show that ASCT2-mediated serine uptake is essential for purine nucleotide biosynthesis and that ERα promotes serine uptake by directly activating SLC1A5 transcription. Together, our work defines an additional important role for ASCT2 as a serine transporter in cancer and evaluates ASCT2 as a potential therapeutic target.","INSTITUTE":"University of Illinois Chicago","DEPARTMENT":"Physiology and Biophysics","LABORATORY":"Coloff Lab","LAST_NAME":"Conger","FIRST_NAME":"Kelly","ADDRESS":"909 S Wolcott Ave, Chicago, IL, 60612","EMAIL":"kconge2@uic.edu","PHONE":"2314320406"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENDER":"Female","CELL_STRAIN_DETAILS":"MCF7","CELL_PRIMARY_IMMORTALIZED":"Immortalized"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"sgLuc IM 1",
"Sample ID":"sgLuc IM 1",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc IM 1.D"}
},
{
"Subject ID":"sgLuc IM 2",
"Sample ID":"sgLuc IM 2",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc IM 2.D"}
},
{
"Subject ID":"sgLuc IM 3",
"Sample ID":"sgLuc IM 3",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc IM 3.D"}
},
{
"Subject ID":"sgSLC1A5-1 IM 1",
"Sample ID":"sgSLC1A5-1 IM 1",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-1 IM 1.D"}
},
{
"Subject ID":"sgSLC1A5-1 IM 2",
"Sample ID":"sgSLC1A5-1 IM 2",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-1 IM 2.D"}
},
{
"Subject ID":"sgSLC1A5-1 IM 3",
"Sample ID":"sgSLC1A5-1 IM 3",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-1 IM 3.D"}
},
{
"Subject ID":"sgSLC1A5-3 IM 1",
"Sample ID":"sgSLC1A5-3 IM 1",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-3 IM 1.D"}
},
{
"Subject ID":"sgSLC1A5-3 IM 2",
"Sample ID":"sgSLC1A5-3 IM 2",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-3 IM 2.D"}
},
{
"Subject ID":"sgSLC1A5-3 IM 3",
"Sample ID":"sgSLC1A5-3 IM 3",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"50uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-3 IM 3.D"}
},
{
"Subject ID":"Luc Puro 1",
"Sample ID":"Luc Puro 1",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc Puro 1.D"}
},
{
"Subject ID":"Luc Puro 2",
"Sample ID":"Luc Puro 2",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc Puro 2.D"}
},
{
"Subject ID":"Luc Puro 3",
"Sample ID":"Luc Puro 3",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc Puro 3.D"}
},
{
"Subject ID":"SLC1A5-1,1",
"Sample ID":"SLC1A5-1,1",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-1,1.D"}
},
{
"Subject ID":"SLC1A5-1,2",
"Sample ID":"SLC1A5-1,2",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-1,2.D"}
},
{
"Subject ID":"SLC1A5-1,3",
"Sample ID":"SLC1A5-1,3",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-1,3.D"}
},
{
"Subject ID":"SLC1A5-3,1",
"Sample ID":"SLC1A5-3,1",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-3,1.D"}
},
{
"Subject ID":"SLC1A5-3,2",
"Sample ID":"SLC1A5-3,2",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-3,2.D"}
},
{
"Subject ID":"SLC1A5-3,3",
"Sample ID":"SLC1A5-3,3",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"ASCT2-KO","Treatment":"285uM Serine"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgSLC1A5-3,3.D"}
},
{
"Subject ID":"sgLuc -Q 60 1",
"Sample ID":"sgLuc -Gln 60 1",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"(-Gln)1hr"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc -Q 60 1.D"}
},
{
"Subject ID":"sgLuc -Q 60 2",
"Sample ID":"sgLuc -Gln 60 2",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"(-Gln)1hr"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc -Q 60 2.D"}
},
{
"Subject ID":"sgLuc -Q 60 3",
"Sample ID":"sgLuc -Gln 60 3",
"Factors":{"Sample source":"Human breast cancer cells","Genotype":"Ctrl","Treatment":"(-Gln)1hr"},
"Additional sample data":{"Cell line":"MCF7","RAW_FILE_NAME(Raw_File_Name)":"sgLuc -Q 60 3.D"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cells were washed with PBS and fed media containing either complete serine (285uM) or low serine (50uM) for six hours. Cells were washed with saline and harvested in cold GCMS-grade methanol with norvaline diluted in water. Polar metabolites were extracted with the addition of chloroform. Samples were dried under constant air flow for 2 hours.","COLLECTION_PROTOCOL_FILENAME":"CL_PM2024.pdf","SAMPLE_TYPE":"Cultured cells","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"Cells are either control (sgLuc) or ASCT2-KO (sgSLC1A5). All groups were plated in triplicate in a six well plate 48hours prior to treatment. Cells were treated with either complete media, or media containing low serine for six hours, or media lacking glutamine (-Q) for one hour prior to harvesting.","TREATMENT_DOSEDURATION":"1-6 hours","CELL_GROWTH_CONTAINER":"Corning 6-well plates","CELL_MEDIA":"RPMI w/o Glucose, Sodium Pyruvate, Amino acids","CELL_PCT_CONFLUENCE":"80%"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were harvested in cold GCMS-grade methanol with norvaline diluted in water. Polar metabolites were separated with chloroform and air dried for two hours. Samples were then rehydrated in 15uL MOX reagent and heated at 37C for 90 minutes. Then 20uL of TBDMS was added to each sample and heated at 60C for 60 minutes.","SAMPLEPREP_PROTOCOL_FILENAME":"CL_PM2024.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"All samples were analyzed by GC/MS using a HP-5MS Ultra Inert GC column (19091S-433UI, Agilent Technologies) installed in an Agilent 7890B gas chromatograph coupled to an Agilent 5779B mass spectrometer. Helium was used as the carrier gas. One microliter was injected (split inlet) at 280 degrees C. After injection, the GC oven was held at 60 degrees C for 1 minute before ramping to 320 degrees C at 10C/min and held for 9 minutes at the maximum temperature.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890B","COLUMN_NAME":"Agilent HP5-MS (30m x 0.25mm, 0.25 um)","SOLVENT_A":"N/A","SOLVENT_B":"N/A","FLOW_GRADIENT":"N/A","FLOW_RATE":"1.5mL/min","COLUMN_TEMPERATURE":"60-320"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"CL_GCMS2024.pdf"},

"MS":{"INSTRUMENT_NAME":"Agilent 5977B","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"The MS system operated under electron impact ionization mode at 70 eV and the MS source and quadrupole were held at 230 degrees C and 150 degrees C respectively. Peak areas were determined using MassHunter software."},

"MS_METABOLITE_DATA":{
"Units":"Abundance",

"Data":[{"Metabolite":"Norvaline","sgLuc IM 1":"2697956","sgLuc IM 2":"2303871","sgLuc IM 3":"2267103","sgSLC1A5-1 IM 1":"2524417","sgSLC1A5-1 IM 2":"2202538","sgSLC1A5-1 IM 3":"2289605","sgSLC1A5-3 IM 1":"2484960","sgSLC1A5-3 IM 2":"2364121","sgSLC1A5-3 IM 3":"2281996"},{"Metabolite":"alpha-Ketoglutarate","sgLuc IM 1":"10402","sgLuc IM 2":"9888","sgLuc IM 3":"9045","sgSLC1A5-1 IM 1":"12673","sgSLC1A5-1 IM 2":"9879","sgSLC1A5-1 IM 3":"9472","sgSLC1A5-3 IM 1":"11535","sgSLC1A5-3 IM 2":"10254","sgSLC1A5-3 IM 3":"10696"},{"Metabolite":"Aspartic Acid","sgLuc IM 1":"423133","sgLuc IM 2":"360546","sgLuc IM 3":"344866","sgSLC1A5-1 IM 1":"543128","sgSLC1A5-1 IM 2":"476509","sgSLC1A5-1 IM 3":"488161","sgSLC1A5-3 IM 1":"609948","sgSLC1A5-3 IM 2":"633544","sgSLC1A5-3 IM 3":"559695"},{"Metabolite":"Citrate","sgLuc IM 1":"36286","sgLuc IM 2":"33583","sgLuc IM 3":"32984","sgSLC1A5-1 IM 1":"41730","sgSLC1A5-1 IM 2":"34157","sgSLC1A5-1 IM 3":"32315","sgSLC1A5-3 IM 1":"44951","sgSLC1A5-3 IM 2":"46076","sgSLC1A5-3 IM 3":"42901"},{"Metabolite":"Citrulline","sgLuc IM 1":"22268","sgLuc IM 2":"20484","sgLuc IM 3":"18261","sgSLC1A5-1 IM 1":"15692","sgSLC1A5-1 IM 2":"16168","sgSLC1A5-1 IM 3":"17232","sgSLC1A5-3 IM 1":"25030","sgSLC1A5-3 IM 2":"15556","sgSLC1A5-3 IM 3":"17513"},{"Metabolite":"Lactate","sgLuc IM 1":"408296","sgLuc IM 2":"409663","sgLuc IM 3":"374741","sgSLC1A5-1 IM 1":"341478","sgSLC1A5-1 IM 2":"327207","sgSLC1A5-1 IM 3":"292581","sgSLC1A5-3 IM 1":"275397","sgSLC1A5-3 IM 2":"275653","sgSLC1A5-3 IM 3":"246223"},{"Metabolite":"L-Alanine","sgLuc IM 1":"55694","sgLuc IM 2":"51416","sgLuc IM 3":"54068","sgSLC1A5-1 IM 1":"129416","sgSLC1A5-1 IM 2":"113097","sgSLC1A5-1 IM 3":"112447","sgSLC1A5-3 IM 1":"179333","sgSLC1A5-3 IM 2":"187351","sgSLC1A5-3 IM 3":"166781"},{"Metabolite":"L-Asparagine","sgLuc IM 1":"346175","sgLuc IM 2":"223916","sgLuc IM 3":"205439","sgSLC1A5-1 IM 1":"336078","sgSLC1A5-1 IM 2":"272295","sgSLC1A5-1 IM 3":"340272","sgSLC1A5-3 IM 1":"484522","sgSLC1A5-3 IM 2":"478085","sgSLC1A5-3 IM 3":"374410"},{"Metabolite":"L-Cysteine","sgLuc IM 1":"59575","sgLuc IM 2":"38504","sgLuc IM 3":"25806","sgSLC1A5-1 IM 1":"27131","sgSLC1A5-1 IM 2":"21751","sgSLC1A5-1 IM 3":"24296","sgSLC1A5-3 IM 1":"66706","sgSLC1A5-3 IM 2":"50354","sgSLC1A5-3 IM 3":"37462"},{"Metabolite":"L-Glutamate","sgLuc IM 1":"1385417","sgLuc IM 2":"1274150","sgLuc IM 3":"1304122","sgSLC1A5-1 IM 1":"1520223","sgSLC1A5-1 IM 2":"1355443","sgSLC1A5-1 IM 3":"1369860","sgSLC1A5-3 IM 1":"1583715","sgSLC1A5-3 IM 2":"1622297","sgSLC1A5-3 IM 3":"1503952"},{"Metabolite":"L-Glutamine","sgLuc IM 1":"1359422","sgLuc IM 2":"1019394","sgLuc IM 3":"952235","sgSLC1A5-1 IM 1":"1149413","sgSLC1A5-1 IM 2":"1107747","sgSLC1A5-1 IM 3":"1085512","sgSLC1A5-3 IM 1":"1501513","sgSLC1A5-3 IM 2":"1523635","sgSLC1A5-3 IM 3":"1164320"},{"Metabolite":"L-Glycine","sgLuc IM 1":"298081","sgLuc IM 2":"257184","sgLuc IM 3":"263677","sgSLC1A5-1 IM 1":"401319","sgSLC1A5-1 IM 2":"339651","sgSLC1A5-1 IM 3":"349507","sgSLC1A5-3 IM 1":"413485","sgSLC1A5-3 IM 2":"422620","sgSLC1A5-3 IM 3":"387013"},{"Metabolite":"L-Isoleucine","sgLuc IM 1":"713472","sgLuc IM 2":"631024","sgLuc IM 3":"577792","sgSLC1A5-1 IM 1":"563223","sgSLC1A5-1 IM 2":"495352","sgSLC1A5-1 IM 3":"490266","sgSLC1A5-3 IM 1":"688394","sgSLC1A5-3 IM 2":"654675","sgSLC1A5-3 IM 3":"596315"},{"Metabolite":"L-Leucine","sgLuc IM 1":"792415","sgLuc IM 2":"675603","sgLuc IM 3":"640348","sgSLC1A5-1 IM 1":"614344","sgSLC1A5-1 IM 2":"550546","sgSLC1A5-1 IM 3":"533982","sgSLC1A5-3 IM 1":"758907","sgSLC1A5-3 IM 2":"729609","sgSLC1A5-3 IM 3":"648271"},{"Metabolite":"L-Lysine","sgLuc IM 1":"21349","sgLuc IM 2":"18126","sgLuc IM 3":"17151","sgSLC1A5-1 IM 1":"18113","sgSLC1A5-1 IM 2":"15645","sgSLC1A5-1 IM 3":"14640","sgSLC1A5-3 IM 1":"23380","sgSLC1A5-3 IM 2":"21443","sgSLC1A5-3 IM 3":"18667"},{"Metabolite":"L-Methionine","sgLuc IM 1":"80701","sgLuc IM 2":"68333","sgLuc IM 3":"63686","sgSLC1A5-1 IM 1":"62289","sgSLC1A5-1 IM 2":"54426","sgSLC1A5-1 IM 3":"52746","sgSLC1A5-3 IM 1":"73948","sgSLC1A5-3 IM 2":"69724","sgSLC1A5-3 IM 3":"63768"},{"Metabolite":"L-Phenylalanine","sgLuc IM 1":"45170","sgLuc IM 2":"37035","sgLuc IM 3":"35797","sgSLC1A5-1 IM 1":"34580","sgSLC1A5-1 IM 2":"29476","sgSLC1A5-1 IM 3":"28738","sgSLC1A5-3 IM 1":"43748","sgSLC1A5-3 IM 2":"40777","sgSLC1A5-3 IM 3":"37147"},{"Metabolite":"L-Proline","sgLuc IM 1":"63477","sgLuc IM 2":"48227","sgLuc IM 3":"49467","sgSLC1A5-1 IM 1":"61301","sgSLC1A5-1 IM 2":"59238","sgSLC1A5-1 IM 3":"60450","sgSLC1A5-3 IM 1":"73633","sgSLC1A5-3 IM 2":"73136","sgSLC1A5-3 IM 3":"64207"},{"Metabolite":"L-Serine","sgLuc IM 1":"34549","sgLuc IM 2":"28552","sgLuc IM 3":"30886","sgSLC1A5-1 IM 1":"22857","sgSLC1A5-1 IM 2":"19633","sgSLC1A5-1 IM 3":"20472","sgSLC1A5-3 IM 1":"18978","sgSLC1A5-3 IM 2":"15975","sgSLC1A5-3 IM 3":"15181"},{"Metabolite":"L-Threonine","sgLuc IM 1":"62029","sgLuc IM 2":"48035","sgLuc IM 3":"50976","sgSLC1A5-1 IM 1":"40977","sgSLC1A5-1 IM 2":"42288","sgSLC1A5-1 IM 3":"51692","sgSLC1A5-3 IM 1":"46303","sgSLC1A5-3 IM 2":"51378","sgSLC1A5-3 IM 3":"37526"},{"Metabolite":"L-Tyrosine","sgLuc IM 1":"68229","sgLuc IM 2":"61794","sgLuc IM 3":"60444","sgSLC1A5-1 IM 1":"55204","sgSLC1A5-1 IM 2":"48238","sgSLC1A5-1 IM 3":"48572","sgSLC1A5-3 IM 1":"70472","sgSLC1A5-3 IM 2":"66103","sgSLC1A5-3 IM 3":"60378"},{"Metabolite":"L-Valine","sgLuc IM 1":"270634","sgLuc IM 2":"236044","sgLuc IM 3":"222593","sgSLC1A5-1 IM 1":"204315","sgSLC1A5-1 IM 2":"183767","sgSLC1A5-1 IM 3":"177932","sgSLC1A5-3 IM 1":"266505","sgSLC1A5-3 IM 2":"252465","sgSLC1A5-3 IM 3":"224866"},{"Metabolite":"Malate","sgLuc IM 1":"132722","sgLuc IM 2":"120344","sgLuc IM 3":"123263","sgSLC1A5-1 IM 1":"143788","sgSLC1A5-1 IM 2":"129266","sgSLC1A5-1 IM 3":"128085","sgSLC1A5-3 IM 1":"122554","sgSLC1A5-3 IM 2":"126028","sgSLC1A5-3 IM 3":"119490"},{"Metabolite":"OH-Proline","sgLuc IM 1":"60748","sgLuc IM 2":"55818","sgLuc IM 3":"63224","sgSLC1A5-1 IM 1":"63300","sgSLC1A5-1 IM 2":"68379","sgSLC1A5-1 IM 3":"76312","sgSLC1A5-3 IM 1":"86080","sgSLC1A5-3 IM 2":"99550","sgSLC1A5-3 IM 3":"89039"},{"Metabolite":"Pyruvate","sgLuc IM 1":"126663","sgLuc IM 2":"112897","sgLuc IM 3":"102740","sgSLC1A5-1 IM 1":"111891","sgSLC1A5-1 IM 2":"97328","sgSLC1A5-1 IM 3":"93330","sgSLC1A5-3 IM 1":"130419","sgSLC1A5-3 IM 2":"117918","sgSLC1A5-3 IM 3":"102525"}],

"Metabolites":[{"Metabolite":"Norvaline","PubChem ID":"65098"},{"Metabolite":"alpha-Ketoglutarate","PubChem ID":"51"},{"Metabolite":"Aspartic Acid","PubChem ID":"5960"},{"Metabolite":"Citrate","PubChem ID":"31348"},{"Metabolite":"Citrulline","PubChem ID":"9750"},{"Metabolite":"Lactate","PubChem ID":"91435"},{"Metabolite":"L-Alanine","PubChem ID":"5950"},{"Metabolite":"L-Asparagine","PubChem ID":"6267"},{"Metabolite":"L-Cysteine","PubChem ID":"5862"},{"Metabolite":"L-Glutamate","PubChem ID":"33032"},{"Metabolite":"L-Glutamine","PubChem ID":"5961"},{"Metabolite":"L-Glycine","PubChem ID":"750"},{"Metabolite":"L-Isoleucine","PubChem ID":"6306"},{"Metabolite":"L-Leucine","PubChem ID":"6106"},{"Metabolite":"L-Lysine","PubChem ID":"5962"},{"Metabolite":"L-Methionine","PubChem ID":"6137"},{"Metabolite":"L-Phenylalanine","PubChem ID":"6140"},{"Metabolite":"L-Proline","PubChem ID":"145742"},{"Metabolite":"L-Serine","PubChem ID":"5951"},{"Metabolite":"L-Threonine","PubChem ID":"6288"},{"Metabolite":"L-Tyrosine","PubChem ID":"6057"},{"Metabolite":"L-Valine","PubChem ID":"6287"},{"Metabolite":"Malate","PubChem ID":"525"},{"Metabolite":"OH-Proline","PubChem ID":"614"},{"Metabolite":"Pyruvate","PubChem ID":"107735"}]
}

}