{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003310","ANALYSIS_ID":"AN005422","PROJECT_ID":"PR002057","VERSION":"1","CREATED_ON":"July 10, 2024, 8:49 pm"},

"PROJECT":{"PROJECT_TITLE":"Quantifying the concentration of endo-borneol via indoor simulated leaching based on local precenpitation from 2016 to 2021","PROJECT_SUMMARY":"Endo-borneol is a compound identified in the needle leachates of Pinus koraiensis. In the agroforestry system where Panax ginseng is planted under Pinus koraiensis, it is necessary to evaluate the concentration range of endo-borneol due to natural precipitation, as endo-borneol is proved to facilitate the growth and disease resistance in Panax ginseng at specific concentration interval.","INSTITUTE":"Yunnan University","DEPARTMENT":"School of Agriculture","LABORATORY":"Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China","LAST_NAME":"Xing-Yu","FIRST_NAME":"Ji","ADDRESS":"Yuhua Road, Chenggong, Kunming, Yunnan Province, China","EMAIL":"xingyu.ji@hotmail.com","PHONE":"＋86-13354989110"},

"STUDY":{"STUDY_TITLE":"Determination the concentration of endo-borneol from needle leachates of Pinus koraiensis flushed by precipitation via indoor simulated leaching)","STUDY_SUMMARY":"The precipitation from April to October (2016-2021) was provided by local weather burea in Benxi. The monthly precipitation and duration in Benxi is classified into high, medium, and low level. Under each precipitation, the concentration of endo-borneol is quantified by indoor simulated leaching. The simulated leachates were extracted by ethyl acetate and then analyzed by GC/MSD. As a result, the peak area of endo-borneol ranges from 2006.257 to 27106.75 under tested precipitation.","INSTITUTE":"Yunnan University","DEPARTMENT":"School of Agriculture","LABORATORY":"Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China","LAST_NAME":"Xing-Yu","FIRST_NAME":"Ji","ADDRESS":"Road Fengyuan, District Panlong, Kunming, Yunnan, China","EMAIL":"xingyu.ji@hotmail.com","PHONE":"＋86-13354989110"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Pinus koraiensis"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"standard_1",
"Factors":{"Precipitation":"0.05 ng/μL","Sample source":"standard"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"211020-0.05.D"}
},
{
"Subject ID":"-",
"Sample ID":"standard_2",
"Factors":{"Precipitation":"0.1 ng/μL","Sample source":"standard"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"211020-0.1.D"}
},
{
"Subject ID":"-",
"Sample ID":"standard_3",
"Factors":{"Precipitation":"0.2 ng/μL","Sample source":"standard"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"211020-0.2.D"}
},
{
"Subject ID":"-",
"Sample ID":"standard_4",
"Factors":{"Precipitation":"1 ng/μL","Sample source":"standard"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"211020-1.D"}
},
{
"Subject ID":"-",
"Sample ID":"standard_5",
"Factors":{"Precipitation":"2 ng/μL","Sample source":"standard"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"211020-2.D"}
},
{
"Subject ID":"-",
"Sample ID":"H1",
"Factors":{"Precipitation":"high level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-H1.D"}
},
{
"Subject ID":"-",
"Sample ID":"H2",
"Factors":{"Precipitation":"high level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-H2.D"}
},
{
"Subject ID":"-",
"Sample ID":"H3",
"Factors":{"Precipitation":"high level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-H3.D"}
},
{
"Subject ID":"-",
"Sample ID":"H4",
"Factors":{"Precipitation":"high level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-H4.D"}
},
{
"Subject ID":"-",
"Sample ID":"H5",
"Factors":{"Precipitation":"high level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-H5.D"}
},
{
"Subject ID":"-",
"Sample ID":"H6",
"Factors":{"Precipitation":"high level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-H6.D"}
},
{
"Subject ID":"-",
"Sample ID":"L1",
"Factors":{"Precipitation":"low level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-L1.D"}
},
{
"Subject ID":"-",
"Sample ID":"L2",
"Factors":{"Precipitation":"low level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-L2.D"}
},
{
"Subject ID":"-",
"Sample ID":"L3",
"Factors":{"Precipitation":"low level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-L3.D"}
},
{
"Subject ID":"-",
"Sample ID":"L4",
"Factors":{"Precipitation":"low level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-L4.D"}
},
{
"Subject ID":"-",
"Sample ID":"L5",
"Factors":{"Precipitation":"low level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-L5.D"}
},
{
"Subject ID":"-",
"Sample ID":"L6",
"Factors":{"Precipitation":"low level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-L6.D"}
},
{
"Subject ID":"-",
"Sample ID":"M1",
"Factors":{"Precipitation":"medium level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-M1.D"}
},
{
"Subject ID":"-",
"Sample ID":"M2",
"Factors":{"Precipitation":"medium level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-M2.D"}
},
{
"Subject ID":"-",
"Sample ID":"M3",
"Factors":{"Precipitation":"medium level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-M3.D"}
},
{
"Subject ID":"-",
"Sample ID":"M4",
"Factors":{"Precipitation":"medium level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-M4.D"}
},
{
"Subject ID":"-",
"Sample ID":"M5",
"Factors":{"Precipitation":"medium level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-M5.D"}
},
{
"Subject ID":"-",
"Sample ID":"M6",
"Factors":{"Precipitation":"medium level","Sample source":"Pinus koraiensis leaf leachate"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"202133232-M6.D"}
},
{
"Subject ID":"-",
"Sample ID":"solvent_blank",
"Factors":{"Precipitation":"solvent","Sample source":"solvent"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"solvent_blank.D"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Based on historical meteorological data, monthly precipitations were graded as low (34.6 mm, 771.63 min), medium (104.2 mm, 2223.07 min) and high (285.8 mm, 5233.03 min) level. To determine the representative weight of fresh needles per unit area in the forest of P. koraiensis, the tree canopy aboveground within one square meter was trimmed, and the fresh needles therein were weighed at five random points. The average weight (3980.58 g/m2) was then established as the representative weight of fresh needles per square meter. A weight of 84.03 g fresh needles were evenly laid on 2-layers absorbent gauze and fixed at the top of a 5-liter vessel (16.4 cm inside diameter), which was placed on the ground and filled with certain volume of double distilled water for simulated graded rainfall based on graded monthly precipitation. A peristaltic pump with a constant flow rate of 3770 ml/min was used to introduce the water to percolate through the packed fresh needle surface. The simulated leachates were filtered twice with qualitative filter paper via suction filtration and stored at 4 °C for quantification.","SAMPLE_TYPE":"Leaf leachate"},

"TREATMENT":{"TREATMENT_SUMMARY":"A volume of 100 ml of leachates was extracted by 300 ml ethyl acetate then evaporated by rotary evaporator at 45°C with 4°C-condensed water recycled in low-temperature circulator. The remaining sediment was rinsed with 2.5 ml of hexane. The liquid hexane was filtered using a 0.22 um Millipore filter for further analysis."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"After rotary evaporation, the remaining sediment was washed out with 5 ml of ethyl acetate. The ethyl acetate was then filtered with a 0.22 um Millipore filter for analysis. A series concentration of endo-borneol standard were formulated in ethyl acetate to create a standard curve."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"The temperature program started from an initial temperature of 50 ℃, followed by an increase of 12℃/min to 160 ℃.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 8860 GC","COLUMN_NAME":"Agilent HP5-MS (30m x 0.25mm, 0.25 um)","SOLVENT_A":"Not applicable","SOLVENT_B":"Not applicable","FLOW_GRADIENT":"Not applicable","FLOW_RATE":"Not applicable","COLUMN_TEMPERATURE":"50 - 160","INJECTION_TEMPERATURE":"200 ℃"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 5977B","INSTRUMENT_TYPE":"GC/MSD","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"An electron ionization system with ionization energy of 7 eV was used. Helium gas (99.999%) was used as the carrier gas at constant flow rate 1 ml/min and an injection volume of 1 ul was employed. The ion-source temperature was 230 °C. The scan interval of mass spectra was 3.1 units/s and fragments from 35 to 270 da. Afterwards, Agilent MassHunter Workstation Quantitative Analysis B.07.01 were used as analytical software. The mass spectrum of the individual peak was inspected manually to confirm the peak had the correct m/z diagnostic fragments, matched with the search results from NIST MS Search 2.2 library. For endo-borneol, the target ion is m/z 95, and qualifier ions are m/z 110 and m/z 67."},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"endo-borneol","standard_1":"2431","standard_2":"5420","standard_3":"13168","standard_4":"85685","standard_5":"180332","H1":"27106.74664","H2":"21932.49085","H3":"26502.03031","H4":"24828.10107","H5":"25171.98278","H6":"24911.03616","L1":"4959.515829","L2":"5939.907034","L3":"2753.115279","L4":"3068.622912","L5":"2351.844628","L6":"2006.257158","M1":"20873.03626","M2":"11897.40655","M3":"15275.51294","M4":"8956.121666","M5":"16697.36107","M6":"15932.95399","solvent_blank":"0"}],

"Metabolites":[{"metabolite_name":"endo-borneol"}]
}

}