{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003425","ANALYSIS_ID":"AN005624","PROJECT_ID":"PR002117","VERSION":"1","CREATED_ON":"August 21, 2024, 11:28 pm"},

"PROJECT":{"PROJECT_TITLE":"A mouse protozoan boosts antigen-specific mucosal IgA responses in a specific lipid metabolism- and signaling-dependent manner","PROJECT_SUMMARY":"IgA antibodies play an important role in mucosal immunity. However, there is still no effective way to consistently boost mucosal IgA responses, and the factors influencing these responses are not fully understood. We observed that colonization with the murine intestinal symbiotic protozoan Tritrichomonas musculis (T.mu) boosted antigen-specific mucosal IgA responses in wild-type C57BL/6 mice. This enhancement was attributed to the accumulation of free arachidonic acid (ARA) in the intestinal lumen, which served as a signal to stimulate the production of antigen-specific mucosal IgA. When ARA was prevented from undergoing its downstream metabolic transformation using the 5-lipoxygenase inhibitor zileuton or by blocking its downstream biological signaling through genetic deletion of the Leukotriene B4 receptor 1 (Blt1), the T.mu-mediated enhancement of antigen-specific mucosal IgA production was suppressed. Moreover, both T.mu transfer and dietary supplementation of ARA augmented the efficacy of an oral vaccine against Salmonella infection, with this effect being dependent on Blt1. Our findings elucidate a tripartite circuit linking nutrients from the diet or intestinal microbiota, host lipid metabolism, and the mucosal humoral immune response.","INSTITUTE":"Xuzhou medical university","LAST_NAME":"Kou","FIRST_NAME":"Yanbo","ADDRESS":"Tongshan road 209, Xuzhou, Jiangsu, 221004, China","EMAIL":"fightingkyb@163.com","PHONE":"+86-051683262123"},

"STUDY":{"STUDY_TITLE":"Targeted free fatty acids metabolomics studies on serum, cecal content, cecum tissue, chow diet, and T.mu cells.","STUDY_SUMMARY":"Serum, cecal content, cecum tissue samples of murine, chow diet, and T.mu cells were collected to perform the targeted free fatty acids metabolome analysis. The aim of this study was to verify that whether T.mu could release free PUFA (especially ARA) to the intestinal tract of its host by comparing the PUFA concentration in the freshly isolated T.mu cells, chow diet, cecal content and serum of the host (Mus musculus).Based on the result of this targeted free fatty acids metabolomics studies, we found that T.mu could release ARA to the intestinal tract of its host and increase the concentration of ARA in its host''s intestinal tract.","INSTITUTE":"Xuzhou medical university","LAST_NAME":"Kou","FIRST_NAME":"Yanbo","ADDRESS":"Tongshan road 209, Xuzhou, Jiangsu, 221004, China","EMAIL":"fightingkyb@163.com","PHONE":"+86-051683262123"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Not applicable"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"T_mu",
"Factors":{"Sample source":"T_mu cells","Treatment":"isolated from cecal content"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"T_mu.CDF"}
},
{
"Subject ID":"-",
"Sample ID":"Diet",
"Factors":{"Sample source":"chow diet","Treatment":"ground to power"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"Diet.CDF"}
},
{
"Subject ID":"-",
"Sample ID":"Ctrl_serum",
"Factors":{"Sample source":"serum","Treatment":"control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"Ctrl_serum.CDF"}
},
{
"Subject ID":"-",
"Sample ID":"T_mu_serum",
"Factors":{"Sample source":"serum","Treatment":"colonized with Tmu"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"Ctrl_serum.CDF"}
},
{
"Subject ID":"-",
"Sample ID":"Ctrl_cecum",
"Factors":{"Sample source":"cecum tissue","Treatment":"control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"Ctrl_serum.CDF"}
},
{
"Subject ID":"-",
"Sample ID":"T_mu_cecum",
"Factors":{"Sample source":"cecum tissue","Treatment":"colonized with Tmu"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"Ctrl_serum.CDF"}
},
{
"Subject ID":"-",
"Sample ID":"Ctrl_content",
"Factors":{"Sample source":"cecal content","Treatment":"control"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"Ctrl_serum.CDF"}
},
{
"Subject ID":"-",
"Sample ID":"T_mu_content",
"Factors":{"Sample source":"cecal content","Treatment":"colonized with Tmu"},
"Additional sample data":{"RAW_FILE_NAME(Raw file name)":"Ctrl_serum.CDF"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"WT C57/BL6 mice were colonized with or without T.mu for 7 days, and the serum, cecum tissue, cecal content samples were collected on day 7. For T.mu cells sample, T.mu cells were isolated and counted from T.mu positive cecal content. Samples were washed with cold PBS and then flash-frozen in liquid nitrogen.","SAMPLE_TYPE":"Serum, cecum tissue, cecal content, T.mu cells, chow diet"},

"TREATMENT":{"TREATMENT_SUMMARY":"WT C57/BL6 mice were colonized with or without T.mu for 7 days, and the serum, cecum tissue, cecal content samples were collected on day 7. For T.mu cells sample, T.mu cells were isolated and counted from T.mu positive cecal content. For diet sample, the chow diet power was used."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The weighed cecal content, cecum tissue, serum, animal chow diet powder, or enumerated T.mu cells were transferred into new 2 mL EP tubes followed extraction with 500 μL extracting solution [Isopropanol : n-Hexane = 2:3 (V:V)], including 0.2 mg/L internal standard). After homogenized in a ball mill for 4 min at 40 Hz and a 5 min ultrasound treatment, the samples were centrifuged 16200 × g for 15 min at 4 °C. Then the supernatants were transferred into new 2 mL EP tubes followed with nitrogen blow dry. The dried samples were resuspended in 500 μL of methanol: trimethylsilyl diazomethane solution (1:2), after standing 30 min at room temperature, another nitrogen blow dry was performed. Then, the samples were resuspended in 160 μL of n-hexane followed with a centrifugation of 16200 × g for 1 min. Finally, the supernatants were transferred into new vials for GC-MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"DB-FastFAME capillary column (90 m × 0.25 mm × 0.25 µm, Agilent Technologies)","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890B","COLUMN_NAME":"Agilent DB-FastFAME capillary column (90 m × 0.25 mm × 0.25 um)","SOLVENT_A":"-","SOLVENT_B":"-","FLOW_GRADIENT":"-","FLOW_RATE":"-","COLUMN_TEMPERATURE":"230"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 5977B","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"Mass spectrometry data were acquired in Scan/SIM mode over an m/z range of 33-400 after a solvent delay of 7 minutes. Fatty acid methyl esters (FAMEs) were identified by comparing them to commercial FAME standards (ANPEL, Shanghai, China, Cat# CDAA-252). The absolute concentrations of FAMEs were calculated using calibration curves based on the internal standard method."},

"MS_METABOLITE_DATA":{
"Units":"μg/ml for serum, μg/g for cecal content, μg/g for diet, μg/8*10^7 T.mu cells",

"Data":[{"Metabolite":"Oleic acid","T_mu_content":"37627.95","Ctrl_content":"56029.43","Ctrl_cecum":"30279.12","T_mu_cecum":"60759.18","Ctrl_serum":"45231.54","T_mu_serum":"32806.51","T_mu":"19580.48","Diet":"466521.47"},{"Metabolite":"Palmitic Acid","T_mu_content":"75445.56","Ctrl_content":"67998.47","Ctrl_cecum":"34576.42","T_mu_cecum":"53400.25","Ctrl_serum":"80887","T_mu_serum":"59882.24","T_mu":"95952.18","Diet":"529736.32"},{"Metabolite":"Octanoic Acid","T_mu_content":"27.6","Ctrl_content":"5.54","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"40.56","T_mu_serum":"31.08","T_mu":"36.32","Diet":"1835.27"},{"Metabolite":"Decanoic Acid","T_mu_content":"54.17","Ctrl_content":"25.38","Ctrl_cecum":"39.07","T_mu_cecum":"29.52","Ctrl_serum":"31.14","T_mu_serum":"36.76","T_mu":"3506","Diet":"49.82"},{"Metabolite":"Elaidic acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"81.73","T_mu_serum":"41.62","T_mu":"4192.23","Diet":"0"},{"Metabolite":"Lauric Acid","T_mu_content":"443.29","Ctrl_content":"20.22","Ctrl_cecum":"86.38","T_mu_cecum":"91.78","Ctrl_serum":"13.72","T_mu_serum":"5.14","T_mu":"91.26","Diet":"642.04"},{"Metabolite":"Linoleic acid","T_mu_content":"69682.13","Ctrl_content":"69057.69","Ctrl_cecum":"23288.7","T_mu_cecum":"50446.44","Ctrl_serum":"70355.06","T_mu_serum":"64783.42","T_mu":"66078.22","Diet":"1361381.6"},{"Metabolite":"Myristic Acid","T_mu_content":"2204.33","Ctrl_content":"1819.72","Ctrl_cecum":"650.5","T_mu_cecum":"1825.61","Ctrl_serum":"1181.47","T_mu_serum":"599.61","T_mu":"1548.93","Diet":"25005.82"},{"Metabolite":"Pentadecanoic Acid","T_mu_content":"3562.1","Ctrl_content":"1144.14","Ctrl_cecum":"451.22","T_mu_cecum":"686.58","Ctrl_serum":"434.63","T_mu_serum":"281.83","T_mu":"4213.02","Diet":"4075.64"},{"Metabolite":"Stearic Acid","T_mu_content":"66344.88","Ctrl_content":"38976.5","Ctrl_cecum":"29705.75","T_mu_cecum":"30269.88","Ctrl_serum":"35981.51","T_mu_serum":"30912.09","T_mu":"45962","Diet":"204561.27"},{"Metabolite":"Tridecylic Acid","T_mu_content":"237.73","Ctrl_content":"28.09","Ctrl_cecum":"4.66","T_mu_cecum":"34.19","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"36.8","Diet":"152.76"},{"Metabolite":"Behenic Acid","T_mu_content":"8345.2","Ctrl_content":"5311.53","Ctrl_cecum":"393.16","T_mu_cecum":"609.45","Ctrl_serum":"632.88","T_mu_serum":"345.05","T_mu":"2157.86","Diet":"18918.39"},{"Metabolite":"Undecanoic Acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"6.01","T_mu_cecum":"0.23","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"12.18","Diet":"13.12"},{"Metabolite":"all-cis-5,8,11,14-Eicosatetraenoic Acid","T_mu_content":"16445.13","Ctrl_content":"3779.78","Ctrl_cecum":"19633.23","T_mu_cecum":"21338.07","Ctrl_serum":"18269.15","T_mu_serum":"16387.36","T_mu":"13631.63","Diet":"6265.68"},{"Metabolite":"Tricosanoic Acid","T_mu_content":"1534.17","Ctrl_content":"1214.7","Ctrl_cecum":"71.96","T_mu_cecum":"63.71","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"288.27","Diet":"1558.46"},{"Metabolite":"Linolenic acid","T_mu_content":"3233.24","Ctrl_content":"4060.03","Ctrl_cecum":"548.59","T_mu_cecum":"1734.04","Ctrl_serum":"2401.3","T_mu_serum":"2538.86","T_mu":"3067.46","Diet":"254061.25"},{"Metabolite":"all-cis-4,7,10,13,16-Docosapentaenoic Acid","T_mu_content":"195.99","Ctrl_content":"93.67","Ctrl_cecum":"516.03","T_mu_cecum":"503.7","Ctrl_serum":"548.96","T_mu_serum":"438.07","T_mu":"282.89","Diet":"0"},{"Metabolite":"all-cis-5,8,11,14,17-Eicosapentaenoic Acid","T_mu_content":"1057.51","Ctrl_content":"521.05","Ctrl_cecum":"959.08","T_mu_cecum":"778.96","Ctrl_serum":"1052.08","T_mu_serum":"878.15","T_mu":"1197.67","Diet":"24629.76"},{"Metabolite":"Myristoleic acid","T_mu_content":"1311.1","Ctrl_content":"150.44","Ctrl_cecum":"35.96","T_mu_cecum":"60.21","Ctrl_serum":"107.44","T_mu_serum":"58.96","T_mu":"232.32","Diet":"1000.81"},{"Metabolite":"Lignoceric Acid","T_mu_content":"6128.87","Ctrl_content":"4398.34","Ctrl_cecum":"314.45","T_mu_cecum":"362.33","Ctrl_serum":"59.37","T_mu_serum":"45.42","T_mu":"708.52","Diet":"5689.14"},{"Metabolite":"Erucic acid","T_mu_content":"2234.25","Ctrl_content":"844.42","Ctrl_cecum":"178.27","T_mu_cecum":"421.8","Ctrl_serum":"122.32","T_mu_serum":"130.02","T_mu":"476.68","Diet":"2279.46"},{"Metabolite":"cis-6-Octadecenoic acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"2976.87","Diet":"0"},{"Metabolite":"all-cis-4,7,10,13,16,19-Docosahexaenoic Acid","T_mu_content":"6828.01","Ctrl_content":"1291.61","Ctrl_cecum":"6227.32","T_mu_cecum":"7617.97","Ctrl_serum":"17286.6","T_mu_serum":"14454.94","T_mu":"6893.52","Diet":"17846.87"},{"Metabolite":"Arachidic Acid","T_mu_content":"6570.67","Ctrl_content":"5039.45","Ctrl_cecum":"372.68","T_mu_cecum":"455.85","Ctrl_serum":"111.98","T_mu_serum":"86.35","T_mu":"1320.62","Diet":"11317"},{"Metabolite":"all-cis-7,10,13,16-Docosatetraenoic Acid","T_mu_content":"942.63","Ctrl_content":"303.98","Ctrl_cecum":"1978.27","T_mu_cecum":"2439.37","Ctrl_serum":"337.17","T_mu_serum":"410.5","T_mu":"394.25","Diet":"879.1"},{"Metabolite":"cis-11-Eicosenoic Acid","T_mu_content":"3492.32","Ctrl_content":"2759.65","Ctrl_cecum":"526.05","T_mu_cecum":"1602.77","Ctrl_serum":"1415.14","T_mu_serum":"872.79","T_mu":"851.33","Diet":"29203.73"},{"Metabolite":"Heptadecanoic Acid","T_mu_content":"2452.18","Ctrl_content":"1665.55","Ctrl_cecum":"893.6","T_mu_cecum":"855.64","Ctrl_serum":"803.85","T_mu_serum":"630.46","T_mu":"2490.08","Diet":"2642.19"},{"Metabolite":"Heneicosanoic Acid","T_mu_content":"1026.04","Ctrl_content":"962.22","Ctrl_cecum":"48.68","T_mu_cecum":"82.18","Ctrl_serum":"22.29","T_mu_serum":"10.25","T_mu":"241.63","Diet":"1065.95"},{"Metabolite":"15-Nervonic acid","T_mu_content":"2031.72","Ctrl_content":"804.39","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"6152.66"},{"Metabolite":"cis,cis,cis-8,11,14-Linolenic Acid","T_mu_content":"2650.28","Ctrl_content":"1001.68","Ctrl_cecum":"2993.7","T_mu_cecum":"3405.89","Ctrl_serum":"4047.14","T_mu_serum":"2135.56","T_mu":"1534.45","Diet":"1163.22"},{"Metabolite":"gamma-Linolenic acid","T_mu_content":"205.71","Ctrl_content":"134.08","Ctrl_cecum":"238.15","T_mu_cecum":"383.13","Ctrl_serum":"725.47","T_mu_serum":"917.38","T_mu":"84.89","Diet":"1236.35"},{"Metabolite":"Palmitoleic acid","T_mu_content":"3071.94","Ctrl_content":"3714.46","Ctrl_cecum":"2444.58","T_mu_cecum":"8255.91","Ctrl_serum":"8680.01","T_mu_serum":"3255.93","T_mu":"1491.37","Diet":"62498.55"},{"Metabolite":"trans-11-Octadecenoic acid","T_mu_content":"49515.3","Ctrl_content":"37244.25","Ctrl_cecum":"1231.04","T_mu_cecum":"3722.62","Ctrl_serum":"283.07","T_mu_serum":"41","T_mu":"48051.64","Diet":"0"},{"Metabolite":"cis,cis-11,14-Eicosadienoic Acid","T_mu_content":"861.62","Ctrl_content":"408.81","Ctrl_cecum":"1005.83","T_mu_cecum":"1128.37","Ctrl_serum":"1084.43","T_mu_serum":"561.85","T_mu":"296.75","Diet":"3764.44"},{"Metabolite":"Linoelaidic acid","T_mu_content":"22.18","Ctrl_content":"115.66","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"118.66","Diet":"454.48"},{"Metabolite":"all-cis-7,10,13,16,19-Docosapentaenoic acid","T_mu_content":"461.11","Ctrl_content":"234.58","Ctrl_cecum":"1048.62","T_mu_cecum":"1224.34","Ctrl_serum":"917.42","T_mu_serum":"674.85","T_mu":"257.9","Diet":"3799.36"},{"Metabolite":"Palmitelaidic acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"67.69","T_mu_cecum":"1356.86","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"4794.52"},{"Metabolite":"cis-10-Heptadecenoic Acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"30.87","T_mu_cecum":"349.12","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"0"},{"Metabolite":"all-cis-11,14,17-Eicosatrienoic Acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"38.3","T_mu_cecum":"44.67","Ctrl_serum":"54.19","T_mu_serum":"41","T_mu":"148.8","Diet":"375.3"},{"Metabolite":"cis,cis-13,16-Docosadienoic Acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"85.38"},{"Metabolite":"Myristelaidic acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"170.45"},{"Metabolite":"cis-11-Octadecenoic acid","T_mu_content":"9206.95","Ctrl_content":"6149.43","Ctrl_cecum":"6730.93","T_mu_cecum":"9885.31","Ctrl_serum":"7274.64","T_mu_serum":"3609.61","T_mu":"3059.87","Diet":"96145.78"},{"Metabolite":"trans-10-Heptadecenoic acid","T_mu_content":"52.3","Ctrl_content":"23.25","Ctrl_cecum":"91.48","T_mu_cecum":"84.6","Ctrl_serum":"64.35","T_mu_serum":"61.48","T_mu":"62.32","Diet":"0"},{"Metabolite":"trans-13-Docosenoic acid","T_mu_content":"295.9","Ctrl_content":"278.47","Ctrl_cecum":"64.15","T_mu_cecum":"72.98","Ctrl_serum":"93.81","T_mu_serum":"46.44","T_mu":"296.2","Diet":"3967.64"},{"Metabolite":"6-Octadecenoic acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"0"},{"Metabolite":"cis-10-Pentadecenoic Acid","T_mu_content":"342.16","Ctrl_content":"235.57","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"291.24","Diet":"0"},{"Metabolite":"trans-10-pentadecenoic acid","T_mu_content":"0","Ctrl_content":"0","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"0"},{"Metabolite":"trans-10-Nonadecenoic acid","T_mu_content":"401.22","Ctrl_content":"444.27","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"62.2","T_mu_serum":"79.64","T_mu":"295.49","Diet":"0"},{"Metabolite":"trans-11-Eicosenoic acid","T_mu_content":"182.12","Ctrl_content":"111.14","Ctrl_cecum":"0","T_mu_cecum":"0","Ctrl_serum":"0","T_mu_serum":"0","T_mu":"0","Diet":"0"}],

"Metabolites":[{"Metabolite":"Oleic acid","CAS":"112-80-1","Molecular weight":"282.4614","KEGG ID":"C00712"},{"Metabolite":"Palmitic Acid","CAS":"57-10-3","Molecular weight":"256.4241","KEGG ID":"C00249"},{"Metabolite":"Octanoic Acid","CAS":"124-07-2","Molecular weight":"144.21","KEGG ID":"C06423"},{"Metabolite":"Decanoic Acid","CAS":"334-48-5","Molecular weight":"172.26","KEGG ID":"C01571"},{"Metabolite":"Elaidic acid","CAS":"112-79-8","Molecular weight":"282.4614","KEGG ID":"C01712"},{"Metabolite":"Lauric Acid","CAS":"143-07-7","Molecular weight":"200.32","KEGG ID":"C02679"},{"Metabolite":"Linoleic acid","CAS":"60-33-3","Molecular weight":"280.4455","KEGG ID":"C01595"},{"Metabolite":"Myristic Acid","CAS":"544-63-8","Molecular weight":"228.37","KEGG ID":"C06424"},{"Metabolite":"Pentadecanoic Acid","CAS":"1002-84-2","Molecular weight":"242.4","KEGG ID":"C16537"},{"Metabolite":"Stearic Acid","CAS":"57-11-4","Molecular weight":"284.4772","KEGG ID":"C01530"},{"Metabolite":"Tridecylic Acid","CAS":"638-53-9","Molecular weight":"214.34","KEGG ID":"C17076"},{"Metabolite":"Behenic Acid","CAS":"112-85-6","Molecular weight":"340.5836","KEGG ID":"C08281"},{"Metabolite":"Undecanoic Acid","CAS":"112-37-8","Molecular weight":"186.291","KEGG ID":"C17715"},{"Metabolite":"all-cis-5,8,11,14-Eicosatetraenoic Acid","CAS":"506-32-1","Molecular weight":"304.4669","KEGG ID":"C00219"},{"Metabolite":"Tricosanoic Acid","CAS":"2433-96-7","Molecular weight":"354.61","KEGG ID":"NA"},{"Metabolite":"Linolenic acid","CAS":"463-40-1","Molecular weight":"278.4296","KEGG ID":"C06427"},{"Metabolite":"all-cis-4,7,10,13,16-Docosapentaenoic Acid","CAS":"25182-74-5","Molecular weight":"330.5","KEGG ID":"C16513"},{"Metabolite":"all-cis-5,8,11,14,17-Eicosapentaenoic Acid","CAS":"10417-94-4","Molecular weight":"302.451","KEGG ID":"C06428"},{"Metabolite":"Myristoleic acid","CAS":"544-64-9","Molecular weight":"226.355","KEGG ID":"C08322"},{"Metabolite":"Lignoceric Acid","CAS":"557-59-5","Molecular weight":"368.6367","KEGG ID":"C08320"},{"Metabolite":"Erucic acid","CAS":"112-86-7","Molecular weight":"338.5677","KEGG ID":"C08316"},{"Metabolite":"cis-6-Octadecenoic acid","CAS":"593-39-5","Molecular weight":"282.4614","KEGG ID":"C08363"},{"Metabolite":"all-cis-4,7,10,13,16,19-Docosahexaenoic Acid","CAS":"6217-54-5","Molecular weight":"328.4883","KEGG ID":"C06429"},{"Metabolite":"Arachidic Acid","CAS":"506-30-9","Molecular weight":"312.5304","KEGG ID":"C06425"},{"Metabolite":"all-cis-7,10,13,16-Docosatetraenoic Acid","CAS":"28874-58-0","Molecular weight":"332.52","KEGG ID":"C16527"},{"Metabolite":"cis-11-Eicosenoic Acid","CAS":"5561-99-9","Molecular weight":"310.5145","KEGG ID":"C16526"},{"Metabolite":"Heptadecanoic Acid","CAS":"506-12-7","Molecular weight":"270.45","KEGG ID":"NA"},{"Metabolite":"Heneicosanoic Acid","CAS":"2363-71-5","Molecular weight":"326.56","KEGG ID":"NA"},{"Metabolite":"15-Nervonic acid","CAS":"506-37-6","Molecular weight":"366.6208","KEGG ID":"C08323"},{"Metabolite":"cis,cis,cis-8,11,14-Linolenic Acid","CAS":"1783-84-2","Molecular weight":"306.4828","KEGG ID":"C03242"},{"Metabolite":"gamma-Linolenic acid","CAS":"506-26-3","Molecular weight":"278.4296","KEGG ID":"C06426"},{"Metabolite":"Palmitoleic acid","CAS":"373-49-9","Molecular weight":"254.4082","KEGG ID":"C08362"},{"Metabolite":"trans-11-Octadecenoic acid","CAS":"693-72-1","Molecular weight":"282.4614","KEGG ID":"C08367"},{"Metabolite":"cis,cis-11,14-Eicosadienoic Acid","CAS":"5598-38-9","Molecular weight":"308.4986","KEGG ID":"C16525"},{"Metabolite":"Linoelaidic acid","CAS":"506-21-8","Molecular weight":"280.4455","KEGG ID":"NA"},{"Metabolite":"all-cis-7,10,13,16,19-Docosapentaenoic acid","CAS":"24880-45-3","Molecular weight":"330.5042","KEGG ID":"C16513"},{"Metabolite":"Palmitelaidic acid","CAS":"10030-74-7","Molecular weight":"254.4082","KEGG ID":"NA"},{"Metabolite":"cis-10-Heptadecenoic Acid","CAS":"29743-97-3","Molecular weight":"268.4348","KEGG ID":"NA"},{"Metabolite":"all-cis-11,14,17-Eicosatrienoic Acid","CAS":"17046-59-2","Molecular weight":"306.4828","KEGG ID":"C16522"},{"Metabolite":"cis,cis-13,16-Docosadienoic Acid","CAS":"17735-98-7","Molecular weight":"336.5518","KEGG ID":"C16533"},{"Metabolite":"Myristelaidic acid","CAS":"50286-30-1","Molecular weight":"226.36","KEGG ID":"NA"},{"Metabolite":"cis-11-Octadecenoic acid","CAS":"506-17-2 ","Molecular weight":"282.46","KEGG ID":"C21944"},{"Metabolite":"trans-10-Heptadecenoic acid","CAS":"126761-43-1","Molecular weight":"268.4348","KEGG ID":"NA"},{"Metabolite":"trans-13-Docosenoic acid","CAS":"506-33-2","Molecular weight":"338.57","KEGG ID":"NA"},{"Metabolite":"6-Octadecenoic acid","CAS":"593-40-8 ","Molecular weight":"282.46","KEGG ID":"NA"},{"Metabolite":"cis-10-Pentadecenoic Acid","CAS":"84743-29-3","Molecular weight":"240.38","KEGG ID":"NA"},{"Metabolite":"trans-10-pentadecenoic acid","CAS":"321744-58-5","Molecular weight":"240.38","KEGG ID":"NA"},{"Metabolite":"trans-10-Nonadecenoic acid","CAS":"67228-95-9","Molecular weight":"296.5","KEGG ID":"NA"},{"Metabolite":"trans-11-Eicosenoic acid","CAS":"62322-84-3","Molecular weight":"310.51","KEGG ID":"NA"}]
}

}