{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003552","ANALYSIS_ID":"AN005839","PROJECT_ID":"PR002188","VERSION":"1","CREATED_ON":"November 6, 2024, 8:10 pm"},

"PROJECT":{"PROJECT_TITLE":"Comprehension of the age-dependent gut and brain interaction of honeybee workers by integration of multi omics approaches","PROJECT_SUMMARY":"In honeybees, division of labour is a key feature, with age-related behavioural transitions being closely associated with molecular changes in the brain, gut, and microbiota. In this study, to investigate these molecular changes and thus better understand their contribution to behavioural responses and modulation, we analysed the global metabolomic shifts in honeybee workers and their microbiota throughout their lives. Overall, our findings provide new insights toward developing potential biomarkers for evaluation of different functional changes related to various environmental stressors.","INSTITUTE":"Helmholtz Centre for Environmental Research","DEPARTMENT":"Molecular Systems Biology","LAST_NAME":"Engelmann","FIRST_NAME":"Beatrice","ADDRESS":"Permoserstraße 15, Leipzipg, Saxony, 03418, Germany","EMAIL":"beatrice.engelmann@ufz.de","PHONE":"004934160251099"},

"STUDY":{"STUDY_TITLE":"Investigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for SCFA determination","STUDY_SUMMARY":"To determine changes in the metabolic activity of the gut microbiota, we measured the concentrations of seven short-chain fatty acids (SCFA) and lactate. All SCFA showed low concentrations in newly emerged workers. We found that acetate, propionate and butyrate are the main SCFA in the honeybee gut and peak in 5-day old and in-hive workers. Lactate showed an inverse trend with highest concentrations in 1-day old workers and a significant decrease on all subsequent days (Kruskal Wallis test, p <0.05).","INSTITUTE":"Helmholtz Centre for Environmental Research","DEPARTMENT":"Molecular Systems Biology","LAST_NAME":"Engelmann","FIRST_NAME":"Beatrice","ADDRESS":"Permoserstraße 15, Leipzipg, Saxony, 03418, Germany","EMAIL":"beatrice.engelmann@ufz.de","PHONE":"004934160251099"},

"SUBJECT":{"SUBJECT_TYPE":"Insect","SUBJECT_SPECIES":"Apis mellifera","TAXONOMY_ID":"7460"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"H1_D0_1",
"Sample ID":"H1_D0_1",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D0_2",
"Sample ID":"H1_D0_2",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D0_3",
"Sample ID":"H1_D0_3",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D0_4",
"Sample ID":"H1_D0_4",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D0_5",
"Sample ID":"H1_D0_5",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D0_1",
"Sample ID":"H2_D0_1",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D0_2",
"Sample ID":"H2_D0_2",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D0_3",
"Sample ID":"H2_D0_3",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D0_4",
"Sample ID":"H2_D0_4",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D0_5",
"Sample ID":"H2_D0_5",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D0_1",
"Sample ID":"H3_D0_1",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D0_2",
"Sample ID":"H3_D0_2",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D0_3",
"Sample ID":"H3_D0_3",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D0_4",
"Sample ID":"H3_D0_4",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D0_5",
"Sample ID":"H3_D0_5",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D1_1",
"Sample ID":"H1_D1_1",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D1_2",
"Sample ID":"H1_D1_2",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D1_3",
"Sample ID":"H1_D1_3",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D1_4",
"Sample ID":"H1_D1_4",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D1_5",
"Sample ID":"H1_D1_5",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D1_1",
"Sample ID":"H2_D1_1",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D1_2",
"Sample ID":"H2_D1_2",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D1_3",
"Sample ID":"H2_D1_3",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D1_4",
"Sample ID":"H2_D1_4",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D1_5",
"Sample ID":"H2_D1_5",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D1_1",
"Sample ID":"H3_D1_1",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D1_2",
"Sample ID":"H3_D1_2",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D1_3",
"Sample ID":"H3_D1_3",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D1_4",
"Sample ID":"H3_D1_4",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D1_5",
"Sample ID":"H3_D1_5",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D3_1",
"Sample ID":"H1_D3_1",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D3_2",
"Sample ID":"H1_D3_2",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D3_3",
"Sample ID":"H1_D3_3",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D3_4",
"Sample ID":"H1_D3_4",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D3_5",
"Sample ID":"H1_D3_5",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D3_1",
"Sample ID":"H2_D3_1",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D3_2",
"Sample ID":"H2_D3_2",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D3_3",
"Sample ID":"H2_D3_3",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D3_4",
"Sample ID":"H2_D3_4",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H2_D3_5",
"Sample ID":"H2_D3_5",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D3_1",
"Sample ID":"H3_D3_1",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D3_2",
"Sample ID":"H3_D3_2",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D3_3",
"Sample ID":"H3_D3_3",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D3_4",
"Sample ID":"H3_D3_4",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H3_D3_5",
"Sample ID":"H3_D3_5",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D5_1",
"Sample ID":"H1_D5_1",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D5_2",
"Sample ID":"H1_D5_2",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D5_3",
"Sample ID":"H1_D5_3",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240227_beegut_Eli_Set1.wiff"}
},
{
"Subject ID":"H1_D5_4",
"Sample ID":"H1_D5_4",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_D5_5",
"Sample ID":"H1_D5_5",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D5_1",
"Sample ID":"H2_D5_1",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D5_2",
"Sample ID":"H2_D5_2",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D5_3",
"Sample ID":"H2_D5_3",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D5_4",
"Sample ID":"H2_D5_4",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D5_5",
"Sample ID":"H2_D5_5",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D5_1",
"Sample ID":"H3_D5_1",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D5_2",
"Sample ID":"H3_D5_2",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D5_3",
"Sample ID":"H3_D5_3",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D5_4",
"Sample ID":"H3_D5_4",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D5_5",
"Sample ID":"H3_D5_5",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_D7_1",
"Sample ID":"H1_D7_1",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_D7_2",
"Sample ID":"H1_D7_2",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_D7_3",
"Sample ID":"H1_D7_3",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_D7_4",
"Sample ID":"H1_D7_4",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_D7_5",
"Sample ID":"H1_D7_5",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D7_1",
"Sample ID":"H2_D7_1",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D7_2",
"Sample ID":"H2_D7_2",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D7_3",
"Sample ID":"H2_D7_3",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D7_4",
"Sample ID":"H2_D7_4",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_D7_5",
"Sample ID":"H2_D7_5",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D7_1",
"Sample ID":"H3_D7_1",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D7_2",
"Sample ID":"H3_D7_2",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D7_3",
"Sample ID":"H3_D7_3",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D7_4",
"Sample ID":"H3_D7_4",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_D7_5",
"Sample ID":"H3_D7_5",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_NF_1",
"Sample ID":"H1_NF_1",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_NF_1",
"Sample ID":"H2_NF_1",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_NF_2",
"Sample ID":"H2_NF_2",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_NF_3",
"Sample ID":"H2_NF_3",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_NF_4",
"Sample ID":"H2_NF_4",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_NF_5",
"Sample ID":"H2_NF_5",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_NF_1",
"Sample ID":"H3_NF_1",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_NF_2",
"Sample ID":"H3_NF_2",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_NF_3",
"Sample ID":"H3_NF_3",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_NF_4",
"Sample ID":"H3_NF_4",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_NF_5",
"Sample ID":"H3_NF_5",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_EF_1",
"Sample ID":"H1_EF_1",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_EF_1",
"Sample ID":"H2_EF_1",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_EF_2",
"Sample ID":"H2_EF_2",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_EF_3",
"Sample ID":"H2_EF_3",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_EF_4",
"Sample ID":"H2_EF_4",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H2_EF_5",
"Sample ID":"H2_EF_5",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_EF_1",
"Sample ID":"H3_EF_1",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_EF_2",
"Sample ID":"H3_EF_2",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_EF_3",
"Sample ID":"H3_EF_3",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_EF_4",
"Sample ID":"H3_EF_4",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H3_EF_5",
"Sample ID":"H3_EF_5",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240229_beegut_Eli_Set2.wiff"}
},
{
"Subject ID":"H1_D0_1",
"Sample ID":"H1_D0_1_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D0_2",
"Sample ID":"H1_D0_2_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D0_3",
"Sample ID":"H1_D0_3_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D0_4",
"Sample ID":"H1_D0_4_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D0_5",
"Sample ID":"H1_D0_5_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D0_1",
"Sample ID":"H2_D0_1_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D0_2",
"Sample ID":"H2_D0_2_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D0_3",
"Sample ID":"H2_D0_3_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D0_4",
"Sample ID":"H2_D0_4_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D0_5",
"Sample ID":"H2_D0_5_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D0_1",
"Sample ID":"H3_D0_1_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D0_2",
"Sample ID":"H3_D0_2_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D0_3",
"Sample ID":"H3_D0_3_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D0_4",
"Sample ID":"H3_D0_4_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D0_5",
"Sample ID":"H3_D0_5_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D1_1",
"Sample ID":"H1_D1_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D1_2",
"Sample ID":"H1_D1_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D1_3",
"Sample ID":"H1_D1_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D1_4",
"Sample ID":"H1_D1_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D1_5",
"Sample ID":"H1_D1_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D1_1",
"Sample ID":"H2_D1_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D1_2",
"Sample ID":"H2_D1_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D1_3",
"Sample ID":"H2_D1_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D1_4",
"Sample ID":"H2_D1_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D1_5",
"Sample ID":"H2_D1_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D1_1",
"Sample ID":"H3_D1_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D1_2",
"Sample ID":"H3_D1_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D1_3",
"Sample ID":"H3_D1_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D1_4",
"Sample ID":"H3_D1_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D1_5",
"Sample ID":"H3_D1_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D3_1",
"Sample ID":"H1_D3_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D3_2",
"Sample ID":"H1_D3_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D3_3",
"Sample ID":"H1_D3_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D3_4",
"Sample ID":"H1_D3_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D3_5",
"Sample ID":"H1_D3_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D3_1",
"Sample ID":"H2_D3_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D3_2",
"Sample ID":"H2_D3_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D3_3",
"Sample ID":"H2_D3_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D3_4",
"Sample ID":"H2_D3_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H2_D3_5",
"Sample ID":"H2_D3_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D3_1",
"Sample ID":"H3_D3_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D3_2",
"Sample ID":"H3_D3_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D3_3",
"Sample ID":"H3_D3_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D3_4",
"Sample ID":"H3_D3_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H3_D3_5",
"Sample ID":"H3_D3_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D5_1",
"Sample ID":"H1_D5_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D5_2",
"Sample ID":"H1_D5_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D5_3",
"Sample ID":"H1_D5_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240304_beegut_Eli_Set1_1v50.wiff"}
},
{
"Subject ID":"H1_D5_4",
"Sample ID":"H1_D5_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_D5_5",
"Sample ID":"H1_D5_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D5_1",
"Sample ID":"H2_D5_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D5_2",
"Sample ID":"H2_D5_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D5_3",
"Sample ID":"H2_D5_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D5_4",
"Sample ID":"H2_D5_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D5_5",
"Sample ID":"H2_D5_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D5_1",
"Sample ID":"H3_D5_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D5_2",
"Sample ID":"H3_D5_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D5_3",
"Sample ID":"H3_D5_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D5_4",
"Sample ID":"H3_D5_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D5_5",
"Sample ID":"H3_D5_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_D7_1",
"Sample ID":"H1_D7_1_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_D7_2",
"Sample ID":"H1_D7_2_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_D7_3",
"Sample ID":"H1_D7_3_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_D7_4",
"Sample ID":"H1_D7_4_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_D7_5",
"Sample ID":"H1_D7_5_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D7_1",
"Sample ID":"H2_D7_1_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D7_2",
"Sample ID":"H2_D7_2_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D7_3",
"Sample ID":"H2_D7_3_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D7_4",
"Sample ID":"H2_D7_4_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_D7_5",
"Sample ID":"H2_D7_5_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D7_1",
"Sample ID":"H3_D7_1_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D7_2",
"Sample ID":"H3_D7_2_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D7_3",
"Sample ID":"H3_D7_3_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D7_4",
"Sample ID":"H3_D7_4_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_D7_5",
"Sample ID":"H3_D7_5_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_NF_1",
"Sample ID":"H1_NF_1_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_NF_1",
"Sample ID":"H2_NF_1_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_NF_2",
"Sample ID":"H2_NF_2_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_NF_3",
"Sample ID":"H2_NF_3_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_NF_4",
"Sample ID":"H2_NF_4_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_NF_5",
"Sample ID":"H2_NF_5_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_NF_1",
"Sample ID":"H3_NF_1_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_NF_2",
"Sample ID":"H3_NF_2_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_NF_3",
"Sample ID":"H3_NF_3_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_NF_4",
"Sample ID":"H3_NF_4_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_NF_5",
"Sample ID":"H3_NF_5_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H1_EF_1",
"Sample ID":"H1_EF_1_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_EF_1",
"Sample ID":"H2_EF_1_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_EF_2",
"Sample ID":"H2_EF_2_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_EF_3",
"Sample ID":"H2_EF_3_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_EF_4",
"Sample ID":"H2_EF_4_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H2_EF_5",
"Sample ID":"H2_EF_5_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_EF_1",
"Sample ID":"H3_EF_1_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_EF_2",
"Sample ID":"H3_EF_2_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_EF_3",
"Sample ID":"H3_EF_3_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_EF_4",
"Sample ID":"H3_EF_4_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
},
{
"Subject ID":"H3_EF_5",
"Sample ID":"H3_EF_5_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"20240306_beegut_Eli_Set2_1v50.wiff"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Workers were pooled to obtain a final sample number of n = 5. The brain(s) and gut(s) from the same workers were assigned the same sample names. In total, we had 15 samples from each age group, with the exception of the foraging stages, where owing to the lack of foragers from Hive 1, we only had 11. All sampled bees were placed directly in liquid nitrogen to prevent changes in the metabolic profile. They were then decapitated in the laboratory so that the head and body of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the stinger apparatus were gently pulled using tweezers, which allowed extraction of the entire gastrointestinal tract. The stinger apparatus and venom sac were carefully excised and the honey crop was removed.","SAMPLE_TYPE":"Bee gut"},

"TREATMENT":{"TREATMENT_SUMMARY":"On the day of newly emerged workers (day 0), bees were sampled as a baseline for no or low bacterial abundance in the gut. Moreover, bees from each hive were collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples for the in-hive worker phase, defined as workers inside the hives that have a fully developed microbiome, were taken every day from Day 7 to Day 10. For the foraging phase, we differentiated between new and experienced foragers, as these show different behaviour due to different levels of experience.Based on experience, they were either collected one to two days after onset (new foragers) or at least four days after onset (experienced foragers)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"In brief, x mg pool gut was mixed with five times the volume (in µL) of acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After centrifugation (2 min, 14000 rpm) 40 µl were used for further derivatization of short chain fatty acids. Each sample was mixed with ACN to a final concentration of 50 %. SCFAs were derivatized with 200 mM 3-nitrophenylhydrazine and 120 mM N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride in pyridine and then diluted in 10 % ACN."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000 RS","COLUMN_NAME":"Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)","SOLVENT_A":"100% water; 0.01% formic acid","SOLVENT_B":"100% acetonitrile; 0.01% formic acid","FLOW_GRADIENT":"0-2min: 15% B, 2-17min: 15-50% B, 17-17.1min: 50-100% B, 17.1-18min: 100% B, 18-18.1min: 100-15% B, 18.1-21min: 15% B","FLOW_RATE":"0.35 mL/min","COLUMN_TEMPERATURE":"40"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5500 QTrap","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"For identification and quantitation, a scheduled MRM method was used, with specific transitions for every SCFA. Data acquisition and peak integration were performed in SciexOS software (Version 3.0.0.). Calculation of concentration was done using external calibration curves and statistics were performed using the R software programme."},

"MS_METABOLITE_DATA":{
"Units":"µMol",

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