#METABOLOMICS WORKBENCH engelmab_20241021_031103 DATATRACK_ID:5297 STUDY_ID:ST003552 ANALYSIS_ID:AN005839 PROJECT_ID:PR002188 VERSION 1 CREATED_ON November 6, 2024, 8:10 pm #PROJECT PR:PROJECT_TITLE Comprehension of the age-dependent gut and brain interaction of honeybee workers PR:PROJECT_TITLE by integration of multi omics approaches PR:PROJECT_SUMMARY In honeybees, division of labour is a key feature, with age-related behavioural PR:PROJECT_SUMMARY transitions being closely associated with molecular changes in the brain, gut, PR:PROJECT_SUMMARY and microbiota. In this study, to investigate these molecular changes and thus PR:PROJECT_SUMMARY better understand their contribution to behavioural responses and modulation, we PR:PROJECT_SUMMARY analysed the global metabolomic shifts in honeybee workers and their microbiota PR:PROJECT_SUMMARY throughout their lives. Overall, our findings provide new insights toward PR:PROJECT_SUMMARY developing potential biomarkers for evaluation of different functional changes PR:PROJECT_SUMMARY related to various environmental stressors. PR:INSTITUTE Helmholtz Centre for Environmental Research PR:DEPARTMENT Molecular Systems Biology PR:LAST_NAME Engelmann PR:FIRST_NAME Beatrice PR:ADDRESS Permoserstraße 15, Leipzipg, Saxony, 03418, Germany PR:EMAIL beatrice.engelmann@ufz.de PR:PHONE 004934160251099 #STUDY ST:STUDY_TITLE Investigation of age-dependent changes in the gut of honeybee workers using ST:STUDY_TITLE targeted metabolomics for SCFA determination ST:STUDY_SUMMARY To determine changes in the metabolic activity of the gut microbiota, we ST:STUDY_SUMMARY measured the concentrations of seven short-chain fatty acids (SCFA) and lactate. ST:STUDY_SUMMARY All SCFA showed low concentrations in newly emerged workers. We found that ST:STUDY_SUMMARY acetate, propionate and butyrate are the main SCFA in the honeybee gut and peak ST:STUDY_SUMMARY in 5-day old and in-hive workers. Lactate showed an inverse trend with highest ST:STUDY_SUMMARY concentrations in 1-day old workers and a significant decrease on all subsequent ST:STUDY_SUMMARY days (Kruskal Wallis test, p <0.05). ST:INSTITUTE Helmholtz Centre for Environmental Research ST:DEPARTMENT Molecular Systems Biology ST:LAST_NAME Engelmann ST:FIRST_NAME Beatrice ST:ADDRESS Permoserstraße 15, Leipzipg, Saxony, 03418, Germany ST:EMAIL beatrice.engelmann@ufz.de ST:PHONE 004934160251099 #SUBJECT SU:SUBJECT_TYPE Insect SU:SUBJECT_SPECIES Apis mellifera SU:TAXONOMY_ID 7460 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS H1_D0_1 H1_D0_1 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff SUBJECT_SAMPLE_FACTORS H1_D0_2 H1_D0_2 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff SUBJECT_SAMPLE_FACTORS H1_D0_3 H1_D0_3 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff SUBJECT_SAMPLE_FACTORS H1_D0_4 H1_D0_4 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; 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RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_NF_2 H3_NF_2 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_NF_3 H3_NF_3 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_NF_4 H3_NF_4 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_NF_5 H3_NF_5 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H1_EF_1 H1_EF_1 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H2_EF_1 H2_EF_1 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H2_EF_2 H2_EF_2 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H2_EF_3 H2_EF_3 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H2_EF_4 H2_EF_4 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H2_EF_5 H2_EF_5 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_EF_1 H3_EF_1 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_EF_2 H3_EF_2 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_EF_3 H3_EF_3 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_EF_4 H3_EF_4 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H3_EF_5 H3_EF_5 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff SUBJECT_SAMPLE_FACTORS H1_D0_1 H1_D0_1_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D0_2 H1_D0_2_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D0_3 H1_D0_3_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D0_4 H1_D0_4_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D0_5 H1_D0_5_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D0_1 H2_D0_1_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D0_2 H2_D0_2_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D0_3 H2_D0_3_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D0_4 H2_D0_4_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D0_5 H2_D0_5_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D0_1 H3_D0_1_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D0_2 H3_D0_2_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D0_3 H3_D0_3_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D0_4 H3_D0_4_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D0_5 H3_D0_5_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D1_1 H1_D1_1_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D1_2 H1_D1_2_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D1_3 H1_D1_3_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D1_4 H1_D1_4_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D1_5 H1_D1_5_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D1_1 H2_D1_1_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D1_2 H2_D1_2_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D1_3 H2_D1_3_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D1_4 H2_D1_4_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D1_5 H2_D1_5_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D1_1 H3_D1_1_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D1_2 H3_D1_2_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D1_3 H3_D1_3_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D1_4 H3_D1_4_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D1_5 H3_D1_5_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D3_1 H1_D3_1_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D3_2 H1_D3_2_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D3_3 H1_D3_3_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D3_4 H1_D3_4_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D3_5 H1_D3_5_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D3_1 H2_D3_1_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D3_2 H2_D3_2_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D3_3 H2_D3_3_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D3_4 H2_D3_4_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D3_5 H2_D3_5_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D3_1 H3_D3_1_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D3_2 H3_D3_2_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D3_3 H3_D3_3_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D3_4 H3_D3_4_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D3_5 H3_D3_5_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D5_1 H1_D5_1_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D5_2 H1_D5_2_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D5_3 H1_D5_3_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D5_4 H1_D5_4_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D5_5 H1_D5_5_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D5_1 H2_D5_1_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D5_2 H2_D5_2_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D5_3 H2_D5_3_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D5_4 H2_D5_4_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D5_5 H2_D5_5_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D5_1 H3_D5_1_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D5_2 H3_D5_2_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D5_3 H3_D5_3_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D5_4 H3_D5_4_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D5_5 H3_D5_5_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D7_1 H1_D7_1_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D7_2 H1_D7_2_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D7_3 H1_D7_3_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D7_4 H1_D7_4_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_D7_5 H1_D7_5_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D7_1 H2_D7_1_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D7_2 H2_D7_2_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D7_3 H2_D7_3_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D7_4 H2_D7_4_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_D7_5 H2_D7_5_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D7_1 H3_D7_1_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D7_2 H3_D7_2_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D7_3 H3_D7_3_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D7_4 H3_D7_4_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_D7_5 H3_D7_5_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_NF_1 H1_NF_1_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_NF_1 H2_NF_1_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_NF_2 H2_NF_2_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_NF_3 H2_NF_3_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_NF_4 H2_NF_4_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_NF_5 H2_NF_5_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_NF_1 H3_NF_1_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_NF_2 H3_NF_2_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_NF_3 H3_NF_3_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_NF_4 H3_NF_4_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_NF_5 H3_NF_5_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H1_EF_1 H1_EF_1_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_EF_1 H2_EF_1_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_EF_2 H2_EF_2_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_EF_3 H2_EF_3_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_EF_4 H2_EF_4_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H2_EF_5 H2_EF_5_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_EF_1 H3_EF_1_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_EF_2 H3_EF_2_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_EF_3 H3_EF_3_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_EF_4 H3_EF_4_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff SUBJECT_SAMPLE_FACTORS H3_EF_5 H3_EF_5_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff #COLLECTION CO:COLLECTION_SUMMARY Workers were pooled to obtain a final sample number of n = 5. The brain(s) and CO:COLLECTION_SUMMARY gut(s) from the same workers were assigned the same sample names. In total, we CO:COLLECTION_SUMMARY had 15 samples from each age group, with the exception of the foraging stages, CO:COLLECTION_SUMMARY where owing to the lack of foragers from Hive 1, we only had 11. All sampled CO:COLLECTION_SUMMARY bees were placed directly in liquid nitrogen to prevent changes in the metabolic CO:COLLECTION_SUMMARY profile. They were then decapitated in the laboratory so that the head and body CO:COLLECTION_SUMMARY of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for CO:COLLECTION_SUMMARY later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the CO:COLLECTION_SUMMARY stinger apparatus were gently pulled using tweezers, which allowed extraction of CO:COLLECTION_SUMMARY the entire gastrointestinal tract. The stinger apparatus and venom sac were CO:COLLECTION_SUMMARY carefully excised and the honey crop was removed. CO:SAMPLE_TYPE Bee gut #TREATMENT TR:TREATMENT_SUMMARY On the day of newly emerged workers (day 0), bees were sampled as a baseline for TR:TREATMENT_SUMMARY no or low bacterial abundance in the gut. Moreover, bees from each hive were TR:TREATMENT_SUMMARY collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples TR:TREATMENT_SUMMARY for the in-hive worker phase, defined as workers inside the hives that have a TR:TREATMENT_SUMMARY fully developed microbiome, were taken every day from Day 7 to Day 10. For the TR:TREATMENT_SUMMARY foraging phase, we differentiated between new and experienced foragers, as these TR:TREATMENT_SUMMARY show different behaviour due to different levels of experience.Based on TR:TREATMENT_SUMMARY experience, they were either collected one to two days after onset (new TR:TREATMENT_SUMMARY foragers) or at least four days after onset (experienced foragers). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY In brief, x mg pool gut was mixed with five times the volume (in µL) of SP:SAMPLEPREP_SUMMARY acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 SP:SAMPLEPREP_SUMMARY Hz, 10 min; Retsch Qiagen). After centrifugation (2 min, 14000 rpm) 40 µl were SP:SAMPLEPREP_SUMMARY used for further derivatization of short chain fatty acids. Each sample was SP:SAMPLEPREP_SUMMARY mixed with ACN to a final concentration of 50 %. SCFAs were derivatized with 200 SP:SAMPLEPREP_SUMMARY mM 3-nitrophenylhydrazine and 120 mM SP:SAMPLEPREP_SUMMARY N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride in pyridine and then SP:SAMPLEPREP_SUMMARY diluted in 10 % ACN. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 RS CH:COLUMN_NAME Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) CH:SOLVENT_A 100% water; 0.01% formic acid CH:SOLVENT_B 100% acetonitrile; 0.01% formic acid CH:FLOW_GRADIENT 0-2min: 15% B, 2-17min: 15-50% B, 17-17.1min: 50-100% B, 17.1-18min: 100% B, CH:FLOW_GRADIENT 18-18.1min: 100-15% B, 18.1-21min: 15% B CH:FLOW_RATE 0.35 mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 5500 QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS For identification and quantitation, a scheduled MRM method was used, with MS:MS_COMMENTS specific transitions for every SCFA. Data acquisition and peak integration were MS:MS_COMMENTS performed in SciexOS software (Version 3.0.0.). Calculation of concentration was MS:MS_COMMENTS done using external calibration curves and statistics were performed using the R MS:MS_COMMENTS software programme. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS µMol MS_METABOLITE_DATA_START Samples H1_D0_1 H1_D0_2 H1_D0_3 H1_D0_4 H1_D0_5 H2_D0_1 H2_D0_2 H2_D0_3 H2_D0_4 H2_D0_5 H3_D0_1 H3_D0_2 H3_D0_3 H3_D0_4 H3_D0_5 H1_D1_1 H1_D1_2 H1_D1_3 H1_D1_4 H1_D1_5 H2_D1_1 H2_D1_2 H2_D1_3 H2_D1_4 H2_D1_5 H3_D1_1 H3_D1_2 H3_D1_3 H3_D1_4 H3_D1_5 H1_D3_1 H1_D3_2 H1_D3_3 H1_D3_4 H1_D3_5 H2_D3_1 H2_D3_2 H2_D3_3 H2_D3_4 H2_D3_5 H3_D3_1 H3_D3_2 H3_D3_3 H3_D3_4 H3_D3_5 H1_D5_1 H1_D5_2 H1_D5_3 H1_D5_4 H1_D5_5 H2_D5_1 H2_D5_2 H2_D5_3 H2_D5_4 H2_D5_5 H3_D5_1 H3_D5_2 H3_D5_3 H3_D5_4 H3_D5_5 H1_D7_1 H1_D7_2 H1_D7_3 H1_D7_4 H1_D7_5 H2_D7_1 H2_D7_2 H2_D7_3 H2_D7_4 H2_D7_5 H3_D7_1 H3_D7_2 H3_D7_3 H3_D7_4 H3_D7_5 H1_NF_1 H2_NF_1 H2_NF_2 H2_NF_3 H2_NF_4 H2_NF_5 H3_NF_1 H3_NF_2 H3_NF_3 H3_NF_4 H3_NF_5 H1_EF_1 H2_EF_1 H2_EF_2 H2_EF_3 H2_EF_4 H2_EF_5 H3_EF_1 H3_EF_2 H3_EF_3 H3_EF_4 H3_EF_5 H1_D0_1_diluted H1_D0_2_diluted H1_D0_3_diluted H1_D0_4_diluted H1_D0_5_diluted H2_D0_1_diluted H2_D0_2_diluted H2_D0_3_diluted H2_D0_4_diluted H2_D0_5_diluted H3_D0_1_diluted H3_D0_2_diluted H3_D0_3_diluted H3_D0_4_diluted H3_D0_5_diluted H1_D1_1_diluted H1_D1_2_diluted H1_D1_3_diluted H1_D1_4_diluted H1_D1_5_diluted H2_D1_1_diluted H2_D1_2_diluted H2_D1_3_diluted H2_D1_4_diluted H2_D1_5_diluted H3_D1_1_diluted H3_D1_2_diluted H3_D1_3_diluted H3_D1_4_diluted H3_D1_5_diluted H1_D3_1_diluted H1_D3_2_diluted H1_D3_3_diluted H1_D3_4_diluted H1_D3_5_diluted H2_D3_1_diluted H2_D3_2_diluted H2_D3_3_diluted H2_D3_4_diluted H2_D3_5_diluted H3_D3_1_diluted H3_D3_2_diluted H3_D3_3_diluted H3_D3_4_diluted H3_D3_5_diluted H1_D5_1_diluted H1_D5_2_diluted H1_D5_3_diluted H1_D5_4_diluted H1_D5_5_diluted H2_D5_1_diluted H2_D5_2_diluted H2_D5_3_diluted H2_D5_4_diluted H2_D5_5_diluted H3_D5_1_diluted H3_D5_2_diluted H3_D5_3_diluted H3_D5_4_diluted H3_D5_5_diluted H1_D7_1_diluted H1_D7_2_diluted H1_D7_3_diluted H1_D7_4_diluted H1_D7_5_diluted H2_D7_1_diluted H2_D7_2_diluted H2_D7_3_diluted H2_D7_4_diluted H2_D7_5_diluted H3_D7_1_diluted H3_D7_2_diluted H3_D7_3_diluted H3_D7_4_diluted H3_D7_5_diluted H1_NF_1_diluted H2_NF_1_diluted H2_NF_2_diluted H2_NF_3_diluted H2_NF_4_diluted H2_NF_5_diluted H3_NF_1_diluted H3_NF_2_diluted H3_NF_3_diluted H3_NF_4_diluted H3_NF_5_diluted H1_EF_1_diluted H2_EF_1_diluted H2_EF_2_diluted H2_EF_3_diluted H2_EF_4_diluted H2_EF_5_diluted H3_EF_1_diluted H3_EF_2_diluted H3_EF_3_diluted H3_EF_4_diluted H3_EF_5_diluted Factors life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut Propionate 1.355 1.665 1.235 1.515 1.955 1.415 1.405 1.37 1.465 1.48 1.885 2.395 2.04 2.215 2.355 2.84 2.735 3.315 3.02 2.72 2.375 1.71 3.13 2.965 3.07 4.305 1.62 2.995 2.755 4.545 14.95 58.5 59.5 29.4 18 44.2 41.85 62 61 61 7.45 28.85 9.15 33.3 29.85 40.55 40 52.5 71.5 34.55 74 70 79.5 55 54 102.5 93 51.5 143 88.5 88.5 166.5 107.5 96.5 45.9 82.5 85.5 93 25.65 67.5 75 94 116 78.5 84 93.5 61 17.65 43.6 17.55 22.8 72.5 55.5 19.65 17.55 12.9 9.85 22.3 56 32.05 2.48 9.4 9.15 11.35 14.35 1.59 14.2 Acetate 16 97.5 13.4 12.75 21.95 18.3 10.1 10.55 10.6 9.8 66.5 46 13.9 20.85 22.05 109.25 322.5 362.5 427.5 492.5 647.5 68.25 520 287.5 690 702.5 183.5 615 522.5 475 900 1250 1542.5 1192.5 1545 1357.5 1317.5 1730 1477.5 1192.5 1240 1417.5 1067.5 1275 1155 967.5 1465 1810 1447.5 812.5 1762.5 1930 2102.5 1857.5 1160 2047.5 2015 1972.5 2092.5 1575 1447.5 1847.5 1787.5 1640 1327.5 1262.5 1345 1147.5 632.5 1322.5 1485 1695 1460 1367.5 1587.5 2220 1170 800 347.5 590 627.5 1035 1112.5 595 457.5 552.5 787.5 580 1485 617.5 159 367.5 700 512.5 557.5 98 485 Butyrate 16 97.5 13.4 12.75 21.95 18.3 10.1 10.55 10.6 9.8 66.5 46 13.9 20.85 22.05 109.25 322.5 362.5 427.5 492.5 647.5 68.25 520 287.5 690 702.5 183.5 615 522.5 475 900 1250 1542.5 1192.5 1545 1357.5 1317.5 1730 1477.5 1192.5 1240 1417.5 1067.5 1275 1155 967.5 1465 1810 1447.5 812.5 1762.5 1930 2102.5 1857.5 1160 2047.5 2015 1972.5 2092.5 1575 1447.5 1847.5 1787.5 1640 1327.5 1262.5 1345 1147.5 632.5 1322.5 1485 1695 1460 1367.5 1587.5 2220 1170 800 347.5 590 627.5 1035 1112.5 595 457.5 552.5 787.5 580 1485 617.5 159 367.5 700 512.5 557.5 98 485 Valerate 1.13 1.385 1.085 1.165 1.285 1.41 1.12 1.125 1.325 1.16 1.825 1.715 1.53 1.51 1.63 1.975 2.275 1.985 3.005 3.025 3.025 1.32 2.26 2.44 2.445 2.65 1.76 2.07 2.21 2.42 8 6.3 13.3 8.2 8.3 4.765 4.94 11.5 6.05 10.85 4.975 8.25 3.065 6.8 11.85 4.825 4.175 10.15 7.75 6.3 11.85 7.5 10.3 7.95 6.35 9.4 10.3 11.15 19.25 6.95 9.5 15.45 11.75 9.6 7.9 8.25 10.95 12 3.19 6.95 11.7 11.2 9.45 10.75 15.9 26.15 6.85 2.775 5.8 2.4 5.8 8.65 7.65 3.62 4.085 4.595 4.385 4.48 5.45 4.605 1.465 3.615 3.235 2.65 5.3 1.945 7.3 Isovalerate 0.94 0.915 0.905 0.89 0.92 0.91 0.925 0.935 0.94 0.915 0.925 0.975 0.985 1.015 0.945 0.935 0.965 0.945 0.995 1.135 1.05 0.89 0.935 1.08 0.915 1.12 0.935 0.97 0.98 1.05 1.295 1.495 1.63 1.375 1.32 1.14 1.415 1.48 1.275 1.625 2.63 2.065 1.535 1.905 1.67 1.34 1.16 1.345 0.97 0.795 1.185 1.12 1.255 1.195 1.38 2.6 2.865 2.87 3.14 3.075 0.765 1.43 1.58 1.46 1.01 1.725 1.795 1.735 1.375 2.085 1.965 2.045 1.67 1.59 2.115 1.585 1.585 1.56 1.82 1.795 1.775 1.535 1.47 1.38 1.085 1.445 2.855 1.94 2.005 2.58 1.82 1.63 1.325 1.255 1.365 1.675 1.18 Isobutyrate 0.313 0.262 0.424 0.955 0.64 0.835 0.062 0.436 0.655 0.302 0.208 0.098 0.202 0.131 0.76 4.775 19.35 6.7 3.215 9.3 5.25 4.47 5.9 8.05 6.6 3.75 1.94 0.7 4.38 2.19 7.25 6.05 6.1 17.6 15.2 14.3 14.6 20.15 11.8 11 14.9 13.4 20.8 16.4 10.4 12.4 14 18.85 10.35 13.9 9.75 10.95 11.9 3.985 16.45 7.2 12.15 8.35 10.55 13.35 25.35 9.05 4.855 10.6 2.795 2.97 7.3 8.9 5.3 4.075 7.35 2.38 2.475 9.5 3.56 0.83 1.88 4.43 4.07 6.45 1.58 5.35 Lactate 10.5 91 7.45 6.3 8.8 5.05 8 5 5.6 5.55 37.3 25.4 6.85 7.25 6.65 290 415 812.5 975 637.5 312.5 118 385 355 765 105.25 257.5 385 224.5 485 21.75 362.5 60 68 59 36 179.5 91.25 44.25 79.25 212.25 229.5 462.5 166 186.25 14.05 373 14.3 34.15 10.95 23.85 15.9 15.75 19.45 80.5 52 34.05 150 21.3 400 97.25 47.5 16.55 39.65 20.55 82 31.3 18.5 32.1 31.9 41.1 26.3 30.15 17.5 25.25 32.25 18.7 37 36.5 157 523 10.75 17.9 175 20.9 43.55 2182.5 1105 48.85 194.25 227.25 395 142.25 99.5 198.5 2.335 108 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name m/z KEGG Pubchem SID Pubchem CID Acetate 194 C00033 3335 176 Propionate 208 C00163 3463 1032 Butyrate 222 C00246 3545 264 Isobutyrate 222 C02632 5612 6590 Valerate 236 C00803 4061 7991 Isovalerate 236 C08262 10461 10430 Lactate 224 C00186 3486 107689 METABOLITES_END #END