{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003553","ANALYSIS_ID":"AN005840","PROJECT_ID":"PR002188","VERSION":"1","CREATED_ON":"November 6, 2024, 8:13 pm"},

"PROJECT":{"PROJECT_TITLE":"Comprehension of the age-dependent gut and brain interaction of honeybee workers by integration of multi omics approaches","PROJECT_SUMMARY":"In honeybees, division of labour is a key feature, with age-related behavioural transitions being closely associated with molecular changes in the brain, gut, and microbiota. In this study, to investigate these molecular changes and thus better understand their contribution to behavioural responses and modulation, we analysed the global metabolomic shifts in honeybee workers and their microbiota throughout their lives. Overall, our findings provide new insights toward developing potential biomarkers for evaluation of different functional changes related to various environmental stressors.","INSTITUTE":"Helmholtz Centre for Environmental Research","DEPARTMENT":"Molecular Systems Biology","LAST_NAME":"Engelmann","FIRST_NAME":"Beatrice","ADDRESS":"Permoserstraße 15, Leipzipg, Saxony, 03418, Germany","EMAIL":"beatrice.engelmann@ufz.de","PHONE":"004934160251099"},

"STUDY":{"STUDY_TITLE":"Investigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for amino acid and biogenic amine determination","STUDY_SUMMARY":"In the honeybee guts we measured the concentrations of 20 amino acids and 6 biogenic amines. Tryptophan and its downstream metabolite kynurenine followed the same trends across the worker development, with a significant decrease in their concentrations from day 3 onwards. Serotonin, on the other hand, was significantly increased from day 1. Tyramine and octopamine showed significant increases in concentrations (octopamine for all age groups apart from Day 1; tyramine for all age groups), while their precursor tyrosine showed significantly decreased concentrations for all age groups except experienced foragers.","INSTITUTE":"Helmholtz Centre for Environmental Research","DEPARTMENT":"Molecular Systems Biology","LAST_NAME":"Engelmann","FIRST_NAME":"Beatrice","ADDRESS":"Permoserstraße 15, Leipzipg, Saxony, 03418, Germany","EMAIL":"beatrice.engelmann@ufz.de","PHONE":"004934160251099"},

"SUBJECT":{"SUBJECT_TYPE":"Insect","SUBJECT_SPECIES":"Apis mellifera","TAXONOMY_ID":"7460"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"H1_D0_1",
"Sample ID":"H1_D0_1",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D0_2",
"Sample ID":"H1_D0_2",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D0_3",
"Sample ID":"H1_D0_3",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D0_4",
"Sample ID":"H1_D0_4",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D0_5",
"Sample ID":"H1_D0_5",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D1_1",
"Sample ID":"H1_D1_1",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D1_2",
"Sample ID":"H1_D1_2",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D1_3",
"Sample ID":"H1_D1_3",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D1_4",
"Sample ID":"H1_D1_4",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D1_5",
"Sample ID":"H1_D1_5",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D3_1",
"Sample ID":"H1_D3_1",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D3_2",
"Sample ID":"H1_D3_2",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D3_3",
"Sample ID":"H1_D3_3",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D3_4",
"Sample ID":"H1_D3_4",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D3_5",
"Sample ID":"H1_D3_5",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D5_1",
"Sample ID":"H1_D5_1",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D5_2",
"Sample ID":"H1_D5_2",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D5_3",
"Sample ID":"H1_D5_3",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D5_4",
"Sample ID":"H1_D5_4",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D5_5",
"Sample ID":"H1_D5_5",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D7_1",
"Sample ID":"H1_D7_1",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D7_2",
"Sample ID":"H1_D7_2",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D7_3",
"Sample ID":"H1_D7_3",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D7_4",
"Sample ID":"H1_D7_4",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D7_5",
"Sample ID":"H1_D7_5",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_NF_1",
"Sample ID":"H1_NF_1",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_EF_1",
"Sample ID":"H1_EF_1",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H1_D0_1",
"Sample ID":"H1_D0_1_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D0_2",
"Sample ID":"H1_D0_2_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D0_3",
"Sample ID":"H1_D0_3_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D0_4",
"Sample ID":"H1_D0_4_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D0_5",
"Sample ID":"H1_D0_5_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D1_1",
"Sample ID":"H1_D1_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D1_2",
"Sample ID":"H1_D1_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D1_3",
"Sample ID":"H1_D1_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D1_4",
"Sample ID":"H1_D1_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D1_5",
"Sample ID":"H1_D1_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D3_1",
"Sample ID":"H1_D3_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D3_2",
"Sample ID":"H1_D3_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D3_3",
"Sample ID":"H1_D3_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D3_4",
"Sample ID":"H1_D3_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D3_5",
"Sample ID":"H1_D3_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D5_1",
"Sample ID":"H1_D5_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D5_2",
"Sample ID":"H1_D5_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D5_3",
"Sample ID":"H1_D5_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D5_4",
"Sample ID":"H1_D5_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D5_5",
"Sample ID":"H1_D5_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D7_1",
"Sample ID":"H1_D7_1_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D7_2",
"Sample ID":"H1_D7_2_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D7_3",
"Sample ID":"H1_D7_3_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D7_4",
"Sample ID":"H1_D7_4_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_D7_5",
"Sample ID":"H1_D7_5_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_NF_1",
"Sample ID":"H1_NF_1_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H1_EF_1",
"Sample ID":"H1_EF_1_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"1","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D0_1",
"Sample ID":"H2_D0_1",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D0_2",
"Sample ID":"H2_D0_2",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D0_3",
"Sample ID":"H2_D0_3",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D0_4",
"Sample ID":"H2_D0_4",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D0_5",
"Sample ID":"H2_D0_5",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D1_1",
"Sample ID":"H2_D1_1",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D1_2",
"Sample ID":"H2_D1_2",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D1_3",
"Sample ID":"H2_D1_3",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D1_4",
"Sample ID":"H2_D1_4",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D1_5",
"Sample ID":"H2_D1_5",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D3_1",
"Sample ID":"H2_D3_1",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D3_2",
"Sample ID":"H2_D3_2",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D3_3",
"Sample ID":"H2_D3_3",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D3_4",
"Sample ID":"H2_D3_4",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D3_5",
"Sample ID":"H2_D3_5",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D5_1",
"Sample ID":"H2_D5_1",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D5_2",
"Sample ID":"H2_D5_2",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D5_3",
"Sample ID":"H2_D5_3",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D5_4",
"Sample ID":"H2_D5_4",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D5_5",
"Sample ID":"H2_D5_5",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D7_1",
"Sample ID":"H2_D7_1",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D7_2",
"Sample ID":"H2_D7_2",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D7_3",
"Sample ID":"H2_D7_3",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D7_4",
"Sample ID":"H2_D7_4",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D7_5",
"Sample ID":"H2_D7_5",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_NF_1",
"Sample ID":"H2_NF_1",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_NF_2",
"Sample ID":"H2_NF_2",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_NF_3",
"Sample ID":"H2_NF_3",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_NF_4",
"Sample ID":"H2_NF_4",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_NF_5",
"Sample ID":"H2_NF_5",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_EF_1",
"Sample ID":"H2_EF_1",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_EF_2",
"Sample ID":"H2_EF_2",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_EF_3",
"Sample ID":"H2_EF_3",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_EF_4",
"Sample ID":"H2_EF_4",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_EF_5",
"Sample ID":"H2_EF_5",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H2_D0_1",
"Sample ID":"H2_D0_1_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D0_2",
"Sample ID":"H2_D0_2_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D0_3",
"Sample ID":"H2_D0_3_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D0_4",
"Sample ID":"H2_D0_4_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D0_5",
"Sample ID":"H2_D0_5_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D1_1",
"Sample ID":"H2_D1_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D1_2",
"Sample ID":"H2_D1_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D1_3",
"Sample ID":"H2_D1_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D1_4",
"Sample ID":"H2_D1_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D1_5",
"Sample ID":"H2_D1_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D3_1",
"Sample ID":"H2_D3_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D3_2",
"Sample ID":"H2_D3_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D3_3",
"Sample ID":"H2_D3_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D3_4",
"Sample ID":"H2_D3_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D3_5",
"Sample ID":"H2_D3_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D5_1",
"Sample ID":"H2_D5_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D5_2",
"Sample ID":"H2_D5_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D5_3",
"Sample ID":"H2_D5_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D5_4",
"Sample ID":"H2_D5_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D5_5",
"Sample ID":"H2_D5_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D7_1",
"Sample ID":"H2_D7_1_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D7_2",
"Sample ID":"H2_D7_2_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D7_3",
"Sample ID":"H2_D7_3_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D7_4",
"Sample ID":"H2_D7_4_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_D7_5",
"Sample ID":"H2_D7_5_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_NF_1",
"Sample ID":"H2_NF_1_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_NF_2",
"Sample ID":"H2_NF_2_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_NF_3",
"Sample ID":"H2_NF_3_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_NF_4",
"Sample ID":"H2_NF_4_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_NF_5",
"Sample ID":"H2_NF_5_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_EF_1",
"Sample ID":"H2_EF_1_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_EF_2",
"Sample ID":"H2_EF_2_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_EF_3",
"Sample ID":"H2_EF_3_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_EF_4",
"Sample ID":"H2_EF_4_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H2_EF_5",
"Sample ID":"H2_EF_5_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"2","RAW_FILE_NAME(Raw_data)":"240229_CU_Eli_Batch1_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D0_1",
"Sample ID":"H3_D0_1",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D0_2",
"Sample ID":"H3_D0_2",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D0_3",
"Sample ID":"H3_D0_3",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D0_4",
"Sample ID":"H3_D0_4",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D0_5",
"Sample ID":"H3_D0_5",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D1_1",
"Sample ID":"H3_D1_1",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D1_2",
"Sample ID":"H3_D1_2",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D1_3",
"Sample ID":"H3_D1_3",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D1_4",
"Sample ID":"H3_D1_4",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D1_5",
"Sample ID":"H3_D1_5",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D3_1",
"Sample ID":"H3_D3_1",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D3_2",
"Sample ID":"H3_D3_2",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D3_3",
"Sample ID":"H3_D3_3",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D3_4",
"Sample ID":"H3_D3_4",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D3_5",
"Sample ID":"H3_D3_5",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D5_1",
"Sample ID":"H3_D5_1",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D5_2",
"Sample ID":"H3_D5_2",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D5_3",
"Sample ID":"H3_D5_3",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D5_4",
"Sample ID":"H3_D5_4",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D5_5",
"Sample ID":"H3_D5_5",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D7_1",
"Sample ID":"H3_D7_1",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D7_2",
"Sample ID":"H3_D7_2",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D7_3",
"Sample ID":"H3_D7_3",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D7_4",
"Sample ID":"H3_D7_4",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D7_5",
"Sample ID":"H3_D7_5",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_NF_1",
"Sample ID":"H3_NF_1",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_NF_2",
"Sample ID":"H3_NF_2",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_NF_3",
"Sample ID":"H3_NF_3",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_NF_4",
"Sample ID":"H3_NF_4",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_NF_5",
"Sample ID":"H3_NF_5",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_EF_1",
"Sample ID":"H3_EF_1",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_EF_2",
"Sample ID":"H3_EF_2",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_EF_3",
"Sample ID":"H3_EF_3",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_EF_4",
"Sample ID":"H3_EF_4",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_EF_5",
"Sample ID":"H3_EF_5",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240319_Eli_YY_Bee_Gut_undiluted.wiff"}
},
{
"Subject ID":"H3_D0_1",
"Sample ID":"H3_D0_1_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D0_2",
"Sample ID":"H3_D0_2_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D0_3",
"Sample ID":"H3_D0_3_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D0_4",
"Sample ID":"H3_D0_4_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D0_5",
"Sample ID":"H3_D0_5_diluted",
"Factors":{"life stage":"newly_emerged","age of workers in days":"0","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D1_1",
"Sample ID":"H3_D1_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D1_2",
"Sample ID":"H3_D1_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D1_3",
"Sample ID":"H3_D1_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D1_4",
"Sample ID":"H3_D1_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D1_5",
"Sample ID":"H3_D1_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"1","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D3_1",
"Sample ID":"H3_D3_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D3_2",
"Sample ID":"H3_D3_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D3_3",
"Sample ID":"H3_D3_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D3_4",
"Sample ID":"H3_D3_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D3_5",
"Sample ID":"H3_D3_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"3","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D5_1",
"Sample ID":"H3_D5_1_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D5_2",
"Sample ID":"H3_D5_2_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D5_3",
"Sample ID":"H3_D5_3_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D5_4",
"Sample ID":"H3_D5_4_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D5_5",
"Sample ID":"H3_D5_5_diluted",
"Factors":{"life stage":"establishment","age of workers in days":"5","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D7_1",
"Sample ID":"H3_D7_1_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D7_2",
"Sample ID":"H3_D7_2_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D7_3",
"Sample ID":"H3_D7_3_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D7_4",
"Sample ID":"H3_D7_4_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_D7_5",
"Sample ID":"H3_D7_5_diluted",
"Factors":{"life stage":"nurse","age of workers in days":"7-10","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_NF_1",
"Sample ID":"H3_NF_1_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_NF_2",
"Sample ID":"H3_NF_2_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_NF_3",
"Sample ID":"H3_NF_3_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_NF_4",
"Sample ID":"H3_NF_4_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_NF_5",
"Sample ID":"H3_NF_5_diluted",
"Factors":{"life stage":"new_foragers","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_EF_1",
"Sample ID":"H3_EF_1_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_EF_2",
"Sample ID":"H3_EF_2_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_EF_3",
"Sample ID":"H3_EF_3_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_EF_4",
"Sample ID":"H3_EF_4_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
},
{
"Subject ID":"H3_EF_5",
"Sample ID":"H3_EF_5_diluted",
"Factors":{"life stage":"experienced_forager","age of workers in days":">12","Sample source":"gut"},
"Additional sample data":{"hive":"3","RAW_FILE_NAME(Raw_data)":"240301_CU_Eli_Batch2_Gut_1zu25.wiff"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Workers were pooled to obtain a final sample number of n = 5. The brain(s) and gut(s) from the same workers were assigned the same sample names. In total, we had 15 samples from each age group, with the exception of the foraging stages, where owing to the lack of foragers from Hive 1, we only had 11. All sampled bees were placed directly in liquid nitrogen to prevent changes in the metabolic profile. They were then decapitated in the laboratory so that the head and body of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the stinger apparatus were gently pulled using tweezers, which allowed extraction of the entire gastrointestinal tract. The stinger apparatus and venom sac were carefully excised and the honey crop was removed.","SAMPLE_TYPE":"Bee gut"},

"TREATMENT":{"TREATMENT_SUMMARY":"On the day of newly emerged workers (day 0), bees were samples as a baseline for no or low bacterial abundance in the gut. Moreover, bees from each hive were collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples for the in-hive worker phase, defined as workers inside the hives that have a fully developed microbiome, were taken every day from Day 7 to Day 10. For the foraging phase, we differentiated between new and experienced foragers, as these show different behaviour due to different levels of experience.Based on experience, they were either collected one to two days after onset (new foragers) or at least four days after onset (experienced foragers)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"In brief, x mg pool gut was mixed with five times the volume (in µL) of acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After Centrifugation (2 min, 14000 rpm), 10 µL were used for amino acid derivatization. First, the supernatant was evaporated to dryness (SpeedVac, Eppendorf), resuspended in 50 µL of 5% phenyl isothiocyanate (PITC) in ethanol: H2O: pyridine (1:1:1, v/v/v), and incubated for 25 min at RT. Subsequently, the samples were dried to remove excess PITC and resuspended in 10 µL 5 mM ammonium acetate in methanol. After incubation (10 min at 14,000 rpm) 90 µL of H2O: ACN + 0.2% formic acid were added. Each derivative was measured in an undiluted and diluted (1:25) manner."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um)","SOLVENT_A":"100% water; 0.2% formic acid","SOLVENT_B":"100% acetonitrile; 0.2% formic acid","FLOW_GRADIENT":"0-0.5 min at 0% B, 0.5-4 min 0-70% B, 4-5.3 min 70% B, 5.3-5.4 min 70-0% B, 5.4-7.3 min 0% B","FLOW_RATE":"0.5 ml/min","COLUMN_TEMPERATURE":"50"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5500 QTrap","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"For identification and quantitation, a scheduled MRM method was used, with specific transitions for every amino acid and biogenic amine. Data acquisition and peak integration were performed in SciexOS software (Version 3.0.0.). Calculation of concentration was done using external calibration curves."},

"MS_METABOLITE_DATA":{
"Units":"µMol",

"Data":[{"Metabolite":"Glycine","H1_D0_1_diluted":"4.25E+02","H1_D0_2_diluted":"6.09E+02","H1_D0_3_diluted":"5.79E+02","H1_D0_4_diluted":"3.90E+02","H1_D0_5_diluted":"4.34E+02","H2_D0_1_diluted":"2.97E+02","H2_D0_2_diluted":"4.01E+02","H2_D0_3_diluted":"3.23E+02","H2_D0_4_diluted":"3.52E+02","H2_D0_5_diluted":"2.66E+02","H3_D0_1_diluted":"3.18E+02","H3_D0_2_diluted":"2.94E+02","H3_D0_3_diluted":"2.88E+02","H3_D0_4_diluted":"3.29E+02","H3_D0_5_diluted":"3.21E+02","H1_D1_1_diluted":"2.93E+02","H1_D1_2_diluted":"2.56E+02","H1_D1_3_diluted":"3.33E+02","H1_D1_4_diluted":"2.48E+02","H1_D1_5_diluted":"2.66E+02","H2_D1_1_diluted":"2.15E+02","H2_D1_2_diluted":"3.54E+02","H2_D1_3_diluted":"2.27E+02","H2_D1_4_diluted":"2.42E+02","H2_D1_5_diluted":"2.44E+02","H3_D1_1_diluted":"4.00E+02","H3_D1_2_diluted":"2.34E+02","H3_D1_3_diluted":"3.33E+02","H3_D1_4_diluted":"3.15E+02","H3_D1_5_diluted":"3.35E+02","H1_D3_1_diluted":"4.62E+02","H1_D3_2_diluted":"5.24E+02","H1_D3_3_diluted":"2.82E+02","H1_D3_4_diluted":"3.39E+02","H1_D3_5_diluted":"3.90E+02","H2_D3_1_diluted":"4.94E+02","H2_D3_2_diluted":"1.89E+02","H2_D3_3_diluted":"2.60E+02","H2_D3_4_diluted":"2.22E+02","H2_D3_5_diluted":"4.30E+02","H3_D3_1_diluted":"2.58E+02","H3_D3_2_diluted":"2.56E+02","H3_D3_3_diluted":"1.61E+02","H3_D3_4_diluted":"2.41E+02","H3_D3_5_diluted":"3.15E+02","H1_D5_1_diluted":"3.33E+02","H1_D5_2_diluted":"2.79E+02","H1_D5_3_diluted":"4.02E+02","H1_D5_4_diluted":"3.11E+02","H1_D5_5_diluted":"3.26E+02","H2_D5_1_diluted":"1.73E+02","H2_D5_2_diluted":"1.45E+02","H2_D5_3_diluted":"2.34E+02","H2_D5_4_diluted":"1.17E+02","H2_D5_5_diluted":"3.07E+02","H3_D5_1_diluted":"2.17E+02","H3_D5_2_diluted":"2.45E+02","H3_D5_3_diluted":"2.15E+02","H3_D5_4_diluted":"1.58E+02","H3_D5_5_diluted":"1.82E+02","H1_D7_1_diluted":"2.39E+02","H1_D7_2_diluted":"1.77E+02","H1_D7_3_diluted":"2.70E+02","H1_D7_4_diluted":"3.30E+02","H1_D7_5_diluted":"2.43E+02","H2_D7_1_diluted":"2.99E+02","H2_D7_2_diluted":"2.80E+02","H2_D7_3_diluted":"2.91E+02","H2_D7_4_diluted":"1.76E+02","H2_D7_5_diluted":"3.63E+02","H3_D7_1_diluted":"2.26E+02","H3_D7_2_diluted":"2.10E+02","H3_D7_3_diluted":"1.95E+02","H3_D7_4_diluted":"2.25E+02","H3_D7_5_diluted":"2.28E+02","H1_NF_1_diluted":"2.49E+02","H2_NF_1_diluted":"2.74E+02","H2_NF_2_diluted":"3.51E+02","H2_NF_3_diluted":"3.87E+02","H2_NF_4_diluted":"2.12E+02","H2_NF_5_diluted":"3.42E+02","H3_NF_1_diluted":"1.64E+02","H3_NF_2_diluted":"1.46E+02","H3_NF_3_diluted":"1.35E+02","H3_NF_4_diluted":"1.51E+02","H3_NF_5_diluted":"1.49E+02","H1_EF_1_diluted":"1.74E+02","H2_EF_1_diluted":"1.44E+02","H2_EF_2_diluted":"2.44E+02","H2_EF_3_diluted":"1.59E+02","H2_EF_4_diluted":"2.05E+02","H2_EF_5_diluted":"1.91E+02","H3_EF_1_diluted":"1.63E+02","H3_EF_2_diluted":"1.29E+02","H3_EF_3_diluted":"1.38E+02","H3_EF_4_diluted":"1.59E+02","H3_EF_5_diluted":"3.05E+02"},{"Metabolite":"Alanine","H1_D0_1_diluted":"3.60E+02","H1_D0_2_diluted":"4.28E+02","H1_D0_3_diluted":"5.03E+02","H1_D0_4_diluted":"3.32E+02","H1_D0_5_diluted":"5.46E+02","H2_D0_1_diluted":"3.22E+02","H2_D0_2_diluted":"3.83E+02","H2_D0_3_diluted":"2.83E+02","H2_D0_4_diluted":"3.13E+02","H2_D0_5_diluted":"2.50E+02","H3_D0_1_diluted":"2.55E+02","H3_D0_2_diluted":"2.42E+02","H3_D0_3_diluted":"2.37E+02","H3_D0_4_diluted":"3.02E+02","H3_D0_5_diluted":"3.02E+02","H1_D1_1_diluted":"2.22E+02","H1_D1_2_diluted":"2.59E+02","H1_D1_3_diluted":"3.23E+02","H1_D1_4_diluted":"2.39E+02","H1_D1_5_diluted":"2.32E+02","H2_D1_1_diluted":"1.22E+02","H2_D1_2_diluted":"3.09E+02","H2_D1_3_diluted":"1.81E+02","H2_D1_4_diluted":"1.72E+02","H2_D1_5_diluted":"1.84E+02","H3_D1_1_diluted":"5.08E+02","H3_D1_2_diluted":"2.16E+02","H3_D1_3_diluted":"3.83E+02","H3_D1_4_diluted":"3.53E+02","H3_D1_5_diluted":"4.09E+02","H1_D3_1_diluted":"4.32E+02","H1_D3_2_diluted":"4.50E+02","H1_D3_3_diluted":"2.31E+02","H1_D3_4_diluted":"2.52E+02","H1_D3_5_diluted":"3.93E+02","H2_D3_1_diluted":"4.50E+02","H2_D3_2_diluted":"1.32E+02","H2_D3_3_diluted":"2.38E+02","H2_D3_4_diluted":"1.29E+02","H2_D3_5_diluted":"3.96E+02","H3_D3_1_diluted":"2.92E+02","H3_D3_2_diluted":"2.84E+02","H3_D3_3_diluted":"1.28E+02","H3_D3_4_diluted":"2.45E+02","H3_D3_5_diluted":"4.01E+02","H1_D5_1_diluted":"2.65E+02","H1_D5_2_diluted":"2.65E+02","H1_D5_3_diluted":"3.07E+02","H1_D5_4_diluted":"3.31E+02","H1_D5_5_diluted":"2.31E+02","H2_D5_1_diluted":"1.42E+02","H2_D5_2_diluted":"1.14E+02","H2_D5_3_diluted":"2.02E+02","H2_D5_4_diluted":"6.25E+01","H2_D5_5_diluted":"2.46E+02","H3_D5_1_diluted":"2.14E+02","H3_D5_2_diluted":"2.56E+02","H3_D5_3_diluted":"2.04E+02","H3_D5_4_diluted":"1.07E+02","H3_D5_5_diluted":"1.80E+02","H1_D7_1_diluted":"2.20E+02","H1_D7_2_diluted":"1.49E+02","H1_D7_3_diluted":"2.25E+02","H1_D7_4_diluted":"4.24E+02","H1_D7_5_diluted":"3.42E+02","H2_D7_1_diluted":"3.16E+02","H2_D7_2_diluted":"2.13E+02","H2_D7_3_diluted":"2.69E+02","H2_D7_4_diluted":"1.23E+02","H2_D7_5_diluted":"4.70E+02","H3_D7_1_diluted":"2.37E+02","H3_D7_2_diluted":"2.51E+02","H3_D7_3_diluted":"2.00E+02","H3_D7_4_dilu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"Metabolites":[{"Metabolite":"Glycine","m/z":"211.2","KEGG":"C00037 ","Pubchem SID":"3339","Pubchem CID":"750"},{"Metabolite":"Alanine","m/z":"225.2","KEGG":"C00041   ","Pubchem SID":"3343","Pubchem CID":"5950"},{"Metabolite":"Serine","m/z":"241.2","KEGG":"C00065","Pubchem SID":"3365","Pubchem CID":"5951"},{"Metabolite":"Valine","m/z":"253.2","KEGG":"C00183 ","Pubchem SID":"3483","Pubchem CID":"6287"},{"Metabolite":"Threonine","m/z":"255.2","KEGG":"C00188   ","Pubchem SID":"3488","Pubchem CID":"6288"},{"Metabolite":"Leucine","m/z":"267.3","KEGG":"C00123   ","Pubchem SID":"3423","Pubchem CID":"6106"},{"Metabolite":"Isoleucine","m/z":"267.3","KEGG":"C00407 ","Pubchem SID":"3697","Pubchem CID":"6306"},{"Metabolite":"Asparagine","m/z":"268.2","KEGG":"C00152    ","Pubchem SID":"3452","Pubchem CID":"6267"},{"Metabolite":"Aspartate","m/z":"269.2","KEGG":"C00049","Pubchem SID":"3351","Pubchem CID":"5960"},{"Metabolite":"Glutamate","m/z":"283.2","KEGG":"C00025  ","Pubchem SID":"3327","Pubchem CID":"33032"},{"Metabolite":"Glutamine","m/z":"282.2","KEGG":"C00064           ","Pubchem SID":"3364","Pubchem CID":"5961"},{"Metabolite":"Methionine","m/z":"185.1","KEGG":"C00073      ","Pubchem SID":"3373","Pubchem CID":"6137"},{"Metabolite":"Histidine","m/z":"291.2","KEGG":"C00135 ","Pubchem SID":"3435","Pubchem CID":"6274"},{"Metabolite":"Phenylalanine","m/z":"301.2","KEGG":"C00079  ","Pubchem SID":"3379","Pubchem CID":"6140"},{"Metabolite":"Arginine","m/z":"310","KEGG":"C00062    ","Pubchem SID":"3362","Pubchem CID":"6322"},{"Metabolite":"Serotonin","m/z":"312.2","KEGG":"C00780","Pubchem SID":"4041","Pubchem CID":"5202"},{"Metabolite":"Tyrosine","m/z":"317.2","KEGG":"C00082","Pubchem SID":"3382","Pubchem CID":"6057"},{"Metabolite":"Tryptophane","m/z":"340.2","KEGG":"C00078     ","Pubchem SID":"3378","Pubchem CID":"6305"},{"Metabolite":"Ornithine","m/z":"403.2","KEGG":"C00077 ","Pubchem SID":"3377","Pubchem CID":"6262"},{"Metabolite":"Lysine","m/z":"417.2","KEGG":"C00047    ","Pubchem SID":"3349","Pubchem CID":"5962"},{"Metabolite":"Gaba","m/z":"239.06","KEGG":"C00334","Pubchem SID":"3628","Pubchem CID":"119"},{"Metabolite":"Kynurenine","m/z":"344.2","KEGG":"C00328 ","Pubchem SID":"3622","Pubchem CID":"161166"},{"Metabolite":"Tyramine","m/z":"273.21","KEGG":"C00483 ","Pubchem SID":"3766","Pubchem CID":"5610"}]
}

}