#METABOLOMICS WORKBENCH engelmab_20241028_063239 DATATRACK_ID:5320 STUDY_ID:ST003553 ANALYSIS_ID:AN005841 PROJECT_ID:PR002188 VERSION 1 CREATED_ON November 6, 2024, 8:13 pm #PROJECT PR:PROJECT_TITLE Comprehension of the age-dependent gut and brain interaction of honeybee workers PR:PROJECT_TITLE by integration of multi omics approaches PR:PROJECT_SUMMARY In honeybees, division of labour is a key feature, with age-related behavioural PR:PROJECT_SUMMARY transitions being closely associated with molecular changes in the brain, gut, PR:PROJECT_SUMMARY and microbiota. In this study, to investigate these molecular changes and thus PR:PROJECT_SUMMARY better understand their contribution to behavioural responses and modulation, we PR:PROJECT_SUMMARY analysed the global metabolomic shifts in honeybee workers and their microbiota PR:PROJECT_SUMMARY throughout their lives. Overall, our findings provide new insights toward PR:PROJECT_SUMMARY developing potential biomarkers for evaluation of different functional changes PR:PROJECT_SUMMARY related to various environmental stressors. PR:INSTITUTE Helmholtz Centre for Environmental Research PR:DEPARTMENT Molecular Systems Biology PR:LAST_NAME Engelmann PR:FIRST_NAME Beatrice PR:ADDRESS Permoserstraße 15, Leipzipg, Saxony, 03418, Germany PR:EMAIL beatrice.engelmann@ufz.de PR:PHONE 004934160251099 #STUDY ST:STUDY_TITLE Investigation of age-dependent changes in the gut of honeybee workers using ST:STUDY_TITLE targeted metabolomics for amino acid and biogenic amine determination ST:STUDY_SUMMARY In the honeybee guts we measured the concentrations of 20 amino acids and 6 ST:STUDY_SUMMARY biogenic amines. Tryptophan and its downstream metabolite kynurenine followed ST:STUDY_SUMMARY the same trends across the worker development, with a significant decrease in ST:STUDY_SUMMARY their concentrations from day 3 onwards. Serotonin, on the other hand, was ST:STUDY_SUMMARY significantly increased from day 1. Tyramine and octopamine showed significant ST:STUDY_SUMMARY increases in concentrations (octopamine for all age groups apart from Day 1; ST:STUDY_SUMMARY tyramine for all age groups), while their precursor tyrosine showed ST:STUDY_SUMMARY significantly decreased concentrations for all age groups except experienced ST:STUDY_SUMMARY foragers. ST:INSTITUTE Helmholtz Centre for Environmental Research ST:DEPARTMENT Molecular Systems Biology ST:LAST_NAME Engelmann ST:FIRST_NAME Beatrice ST:ADDRESS Permoserstraße 15, Leipzipg, Saxony, 03418, Germany ST:EMAIL beatrice.engelmann@ufz.de ST:PHONE 004934160251099 #SUBJECT SU:SUBJECT_TYPE Insect SU:SUBJECT_SPECIES Apis mellifera SU:TAXONOMY_ID 7460 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS H1_D0_1 H1_D0_1 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D0_2 H1_D0_2 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D0_3 H1_D0_3 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D0_4 H1_D0_4 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D0_5 H1_D0_5 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D1_1 H1_D1_1 life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D1_2 H1_D1_2 life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D1_3 H1_D1_3 life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D1_4 H1_D1_4 life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D1_5 H1_D1_5 life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D3_1 H1_D3_1 life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D3_2 H1_D3_2 life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D3_3 H1_D3_3 life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D3_4 H1_D3_4 life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D3_5 H1_D3_5 life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D5_1 H1_D5_1 life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D5_2 H1_D5_2 life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D5_3 H1_D5_3 life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D5_4 H1_D5_4 life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D5_5 H1_D5_5 life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D7_1 H1_D7_1 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D7_2 H1_D7_2 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D7_3 H1_D7_3 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D7_4 H1_D7_4 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D7_5 H1_D7_5 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_NF_1 H1_NF_1 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_EF_1 H1_EF_1 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H1_D0_1 H1_D0_1_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D0_2 H1_D0_2_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D0_3 H1_D0_3_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D0_4 H1_D0_4_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D0_5 H1_D0_5_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D1_1 H1_D1_1_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D1_2 H1_D1_2_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D1_3 H1_D1_3_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D1_4 H1_D1_4_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D1_5 H1_D1_5_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D3_1 H1_D3_1_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D3_2 H1_D3_2_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D3_3 H1_D3_3_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D3_4 H1_D3_4_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D3_5 H1_D3_5_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D5_1 H1_D5_1_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D5_2 H1_D5_2_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D5_3 H1_D5_3_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D5_4 H1_D5_4_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D5_5 H1_D5_5_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D7_1 H1_D7_1_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D7_2 H1_D7_2_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D7_3 H1_D7_3_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D7_4 H1_D7_4_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_D7_5 H1_D7_5_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_NF_1 H1_NF_1_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H1_EF_1 H1_EF_1_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=1; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D0_1 H2_D0_1 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D0_2 H2_D0_2 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D0_3 H2_D0_3 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D0_4 H2_D0_4 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D0_5 H2_D0_5 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D1_1 H2_D1_1 life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D1_2 H2_D1_2 life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D1_3 H2_D1_3 life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D1_4 H2_D1_4 life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D1_5 H2_D1_5 life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D3_1 H2_D3_1 life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D3_2 H2_D3_2 life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D3_3 H2_D3_3 life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D3_4 H2_D3_4 life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D3_5 H2_D3_5 life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D5_1 H2_D5_1 life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D5_2 H2_D5_2 life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D5_3 H2_D5_3 life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D5_4 H2_D5_4 life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D5_5 H2_D5_5 life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D7_1 H2_D7_1 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D7_2 H2_D7_2 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D7_3 H2_D7_3 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D7_4 H2_D7_4 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D7_5 H2_D7_5 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_NF_1 H2_NF_1 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_NF_2 H2_NF_2 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_NF_3 H2_NF_3 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_NF_4 H2_NF_4 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_NF_5 H2_NF_5 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_EF_1 H2_EF_1 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_EF_2 H2_EF_2 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_EF_3 H2_EF_3 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_EF_4 H2_EF_4 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_EF_5 H2_EF_5 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H2_D0_1 H2_D0_1_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D0_2 H2_D0_2_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D0_3 H2_D0_3_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D0_4 H2_D0_4_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D0_5 H2_D0_5_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D1_1 H2_D1_1_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D1_2 H2_D1_2_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D1_3 H2_D1_3_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D1_4 H2_D1_4_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D1_5 H2_D1_5_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D3_1 H2_D3_1_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D3_2 H2_D3_2_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D3_3 H2_D3_3_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D3_4 H2_D3_4_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D3_5 H2_D3_5_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D5_1 H2_D5_1_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D5_2 H2_D5_2_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D5_3 H2_D5_3_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D5_4 H2_D5_4_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D5_5 H2_D5_5_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D7_1 H2_D7_1_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D7_2 H2_D7_2_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D7_3 H2_D7_3_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D7_4 H2_D7_4_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_D7_5 H2_D7_5_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_NF_1 H2_NF_1_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_NF_2 H2_NF_2_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_NF_3 H2_NF_3_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_NF_4 H2_NF_4_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_NF_5 H2_NF_5_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_EF_1 H2_EF_1_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_EF_2 H2_EF_2_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_EF_3 H2_EF_3_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_EF_4 H2_EF_4_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H2_EF_5 H2_EF_5_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=2; RAW_FILE_NAME(Raw_data)=240229_CU_Eli_Batch1_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D0_1 H3_D0_1 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D0_2 H3_D0_2 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D0_3 H3_D0_3 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D0_4 H3_D0_4 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D0_5 H3_D0_5 life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D1_1 H3_D1_1 life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D1_2 H3_D1_2 life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D1_3 H3_D1_3 life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D1_4 H3_D1_4 life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D1_5 H3_D1_5 life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D3_1 H3_D3_1 life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D3_2 H3_D3_2 life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D3_3 H3_D3_3 life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D3_4 H3_D3_4 life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D3_5 H3_D3_5 life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D5_1 H3_D5_1 life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D5_2 H3_D5_2 life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D5_3 H3_D5_3 life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D5_4 H3_D5_4 life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D5_5 H3_D5_5 life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D7_1 H3_D7_1 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D7_2 H3_D7_2 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D7_3 H3_D7_3 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D7_4 H3_D7_4 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D7_5 H3_D7_5 life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_NF_1 H3_NF_1 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_NF_2 H3_NF_2 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_NF_3 H3_NF_3 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_NF_4 H3_NF_4 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_NF_5 H3_NF_5 life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_EF_1 H3_EF_1 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_EF_2 H3_EF_2 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_EF_3 H3_EF_3 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_EF_4 H3_EF_4 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_EF_5 H3_EF_5 life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240319_Eli_YY_Bee_Gut_undiluted.wiff SUBJECT_SAMPLE_FACTORS H3_D0_1 H3_D0_1_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D0_2 H3_D0_2_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D0_3 H3_D0_3_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D0_4 H3_D0_4_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D0_5 H3_D0_5_diluted life stage:newly_emerged | age of workers in days:0 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D1_1 H3_D1_1_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D1_2 H3_D1_2_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D1_3 H3_D1_3_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D1_4 H3_D1_4_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D1_5 H3_D1_5_diluted life stage:establishment | age of workers in days:1 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D3_1 H3_D3_1_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D3_2 H3_D3_2_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D3_3 H3_D3_3_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D3_4 H3_D3_4_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D3_5 H3_D3_5_diluted life stage:establishment | age of workers in days:3 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D5_1 H3_D5_1_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D5_2 H3_D5_2_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D5_3 H3_D5_3_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D5_4 H3_D5_4_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D5_5 H3_D5_5_diluted life stage:establishment | age of workers in days:5 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D7_1 H3_D7_1_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D7_2 H3_D7_2_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D7_3 H3_D7_3_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D7_4 H3_D7_4_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_D7_5 H3_D7_5_diluted life stage:nurse | age of workers in days:7-10 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_NF_1 H3_NF_1_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_NF_2 H3_NF_2_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_NF_3 H3_NF_3_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_NF_4 H3_NF_4_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_NF_5 H3_NF_5_diluted life stage:new_foragers | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_EF_1 H3_EF_1_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_EF_2 H3_EF_2_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_EF_3 H3_EF_3_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_EF_4 H3_EF_4_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff SUBJECT_SAMPLE_FACTORS H3_EF_5 H3_EF_5_diluted life stage:experienced_forager | age of workers in days:>12 | Sample source:gut hive=3; RAW_FILE_NAME(Raw_data)=240301_CU_Eli_Batch2_Gut_1zu25.wiff #COLLECTION CO:COLLECTION_SUMMARY Workers were pooled to obtain a final sample number of n = 5. The brain(s) and CO:COLLECTION_SUMMARY gut(s) from the same workers were assigned the same sample names. In total, we CO:COLLECTION_SUMMARY had 15 samples from each age group, with the exception of the foraging stages, CO:COLLECTION_SUMMARY where owing to the lack of foragers from Hive 1, we only had 11. All sampled CO:COLLECTION_SUMMARY bees were placed directly in liquid nitrogen to prevent changes in the metabolic CO:COLLECTION_SUMMARY profile. They were then decapitated in the laboratory so that the head and body CO:COLLECTION_SUMMARY of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for CO:COLLECTION_SUMMARY later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the CO:COLLECTION_SUMMARY stinger apparatus were gently pulled using tweezers, which allowed extraction of CO:COLLECTION_SUMMARY the entire gastrointestinal tract. The stinger apparatus and venom sac were CO:COLLECTION_SUMMARY carefully excised and the honey crop was removed. CO:SAMPLE_TYPE Bee gut #TREATMENT TR:TREATMENT_SUMMARY On the day of newly emerged workers (day 0), bees were samples as a baseline for TR:TREATMENT_SUMMARY no or low bacterial abundance in the gut. Moreover, bees from each hive were TR:TREATMENT_SUMMARY collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples TR:TREATMENT_SUMMARY for the in-hive worker phase, defined as workers inside the hives that have a TR:TREATMENT_SUMMARY fully developed microbiome, were taken every day from Day 7 to Day 10. For the TR:TREATMENT_SUMMARY foraging phase, we differentiated between new and experienced foragers, as these TR:TREATMENT_SUMMARY show different behaviour due to different levels of experience.Based on TR:TREATMENT_SUMMARY experience, they were either collected one to two days after onset (new TR:TREATMENT_SUMMARY foragers) or at least four days after onset (experienced foragers). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY In brief, x mg pool gut was mixed with five times the volume (in µL) of SP:SAMPLEPREP_SUMMARY acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 SP:SAMPLEPREP_SUMMARY Hz, 10 min; Retsch Qiagen). After Centrifugation (2 min, 14000 rpm), 10 µL were SP:SAMPLEPREP_SUMMARY used for amino acid derivatization. First, the supernatant was evaporated to SP:SAMPLEPREP_SUMMARY dryness (SpeedVac, Eppendorf), resuspended in 50 µL of 5% phenyl isothiocyanate SP:SAMPLEPREP_SUMMARY (PITC) in ethanol: H2O: pyridine (1:1:1, v/v/v), and incubated for 25 min at RT. SP:SAMPLEPREP_SUMMARY Subsequently, the samples were dried to remove excess PITC and resuspended in 10 SP:SAMPLEPREP_SUMMARY µL 5 mM ammonium acetate in methanol. After incubation (10 min at 14,000 rpm) SP:SAMPLEPREP_SUMMARY 90 µL of H2O: ACN + 0.2% formic acid were added. Each derivative was measured SP:SAMPLEPREP_SUMMARY in an undiluted and diluted (1:25) manner. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um) CH:SOLVENT_A 100% water; 0.2% formic acid CH:SOLVENT_B 100% acetonitrile; 0.2% formic acid CH:FLOW_GRADIENT 0-0.5 min at 0% B, 0.5-4 min 0-70% B, 4-5.3 min 70% B, 5.3-5.4 min 70-0% B, CH:FLOW_GRADIENT 5.4-7.3 min 0% B CH:FLOW_RATE 0.5 ml/min CH:COLUMN_TEMPERATURE 50 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 6500+ Qtrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS For identification and quantitation, a scheduled MRM method was used, with MS:MS_COMMENTS specific transitions for every amino acid and biogenic amine. Data acquisition MS:MS_COMMENTS and peak integration were performed in SciexOS software (Version 3.0.0.). MS:MS_COMMENTS Calculation of concentration was done using external calibration curves. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS µMol MS_METABOLITE_DATA_START Samples H1_D0_1 H1_D0_2 H1_D0_3 H1_D0_4 H1_D0_5 H2_D0_1 H2_D0_2 H2_D0_3 H2_D0_4 H2_D0_5 H3_D0_1 H3_D0_2 H3_D0_3 H3_D0_4 H3_D0_5 H1_D1_1 H1_D1_2 H1_D1_3 H1_D1_4 H1_D1_5 H2_D1_1 H2_D1_2 H2_D1_3 H2_D1_4 H2_D1_5 H3_D1_1 H3_D1_2 H3_D1_3 H3_D1_4 H3_D1_5 H1_D3_1 H1_D3_2 H1_D3_3 H1_D3_4 H1_D3_5 H2_D3_1 H2_D3_2 H2_D3_3 H2_D3_4 H2_D3_5 H3_D3_1 H3_D3_2 H3_D3_3 H3_D3_4 H3_D3_5 H1_D5_1 H1_D5_2 H1_D5_3 H1_D5_4 H1_D5_5 H2_D5_1 H2_D5_2 H2_D5_3 H2_D5_4 H2_D5_5 H3_D5_1 H3_D5_2 H3_D5_3 H3_D5_4 H3_D5_5 H1_D7_1 H1_D7_2 H1_D7_3 H1_D7_4 H1_D7_5 H2_D7_1 H2_D7_2 H2_D7_3 H2_D7_4 H2_D7_5 H3_D7_1 H3_D7_2 H3_D7_3 H3_D7_4 H3_D7_5 H1_NF_1 H2_NF_1 H2_NF_2 H2_NF_3 H2_NF_4 H2_NF_5 H3_NF_1 H3_NF_2 H3_NF_3 H3_NF_4 H3_NF_5 H1_EF_1 H2_EF_1 H2_EF_2 H2_EF_3 H2_EF_4 H2_EF_5 H3_EF_1 H3_EF_2 H3_EF_3 H3_EF_4 H3_EF_5 Factors life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:newly_emerged | age of workers in days:0 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:1 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:3 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:establishment | age of workers in days:5 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:nurse | age of workers in days:7-10 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:new_foragers | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut life stage:experienced_forager | age of workers in days:>12 | Sample source:gut Octopamine 7.18E-02 7.73E-02 6.44E-02 6.18E-02 1.16E-01 4.74E-02 4.58E-02 8.66E-02 7.94E-02 7.37E-02 8.68E-02 8.95E-02 1.27E-01 8.00E-02 1.58E-01 7.24E-02 1.11E-01 1.27E-01 8.34E-02 7.70E-02 1.15E-01 1.09E-01 3.88E-01 9.62E-02 8.60E-02 6.46E-02 1.16E-01 2.08E-01 4.63E-02 1.15E-01 2.45E-01 2.21E-01 1.69E-01 2.56E-01 2.47E-01 3.38E-01 1.59E-01 1.51E-01 1.47E-01 2.15E-01 6.79E-02 8.50E-02 8.57E-02 1.10E-01 1.02E-01 3.25E-01 3.75E-01 3.33E-01 3.38E-01 1.66E+00 2.34E-01 2.44E-01 2.50E-01 1.61E-01 3.96E-01 2.17E-01 1.06E-01 7.65E-01 1.90E-01 2.72E-01 3.26E-01 1.05E+00 3.84E-01 3.05E-01 4.30E-01 3.55E-01 2.70E-01 2.92E-01 3.41E-01 2.51E-01 1.44E-01 2.30E-01 2.87E-01 1.51E-01 5.01E-01 3.25E-01 3.16E-01 2.23E-01 2.12E-01 1.24E-01 1.98E-01 1.09E-01 2.43E-01 1.47E-01 1.03E-01 7.55E-02 2.22E-01 2.97E-01 2.97E-01 1.17E-01 2.14E-01 1.94E-01 1.83E-01 8.85E-02 1.57E-01 8.10E-02 4.31E-01 Citrulline 7.46E-01 7.57E-01 7.07E-01 6.42E-01 7.42E-01 5.05E-01 6.30E-01 4.13E-01 4.88E-01 4.47E-01 4.85E-01 8.53E-01 7.16E-01 6.98E-01 7.85E-01 1.24E+00 8.58E-01 8.06E+00 3.37E+00 1.54E+01 1.66E+00 7.78E-01 1.70E+00 1.04E+00 8.15E+00 1.33E+00 4.21E-01 1.26E+00 1.03E+00 3.87E+00 2.67E+01 6.10E+01 1.58E+01 2.93E+01 2.92E+01 6.35E+01 2.77E+01 2.27E+01 2.02E+01 3.24E+01 1.39E+01 1.40E+01 1.32E+01 6.50E+00 8.54E+00 2.10E+01 3.03E+01 2.37E+01 2.98E+01 1.63E+01 2.01E+01 1.01E+01 1.70E+01 8.93E+00 1.21E+01 3.30E+00 4.48E+00 2.80E+00 3.10E+00 4.52E+00 7.99E+00 1.32E+01 7.15E+00 8.80E+00 1.19E+01 2.38E+00 5.80E+00 7.20E+00 4.45E+00 5.60E+00 4.37E+00 4.24E+00 2.55E+00 2.11E+00 3.04E+00 1.69E+01 6.98E+00 6.91E+00 4.53E+00 2.44E+00 5.16E+00 2.20E+00 2.73E+00 1.72E+00 1.78E+00 1.04E+00 6.47E+00 4.33E+00 3.38E+00 2.07E+00 7.69E-01 3.52E-01 1.25E+00 7.18E-01 1.12E+00 2.64E+00 2.76E+00 Dopamine 9.93E-02 3.33E-01 1.25E-01 5.73E-01 2.26E-01 1.94E-01 7.27E-02 2.90E-01 3.54E-01 1.46E-01 3.13E-01 7.06E-02 4.11E-01 2.90E-01 1.55E-01 1.27E+00 8.52E-01 6.96E-01 9.89E-01 2.29E+00 1.70E+00 7.82E-01 1.08E+00 1.68E+00 1.17E+00 2.31E-01 6.50E-01 1.58E+00 3.69E-01 5.61E-01 2.55E+00 3.35E+00 5.11E+00 2.40E+00 2.66E+00 2.24E+00 2.42E+00 3.16E+00 2.93E+00 2.48E+00 1.74E+00 1.91E+00 1.06E+00 1.68E+00 2.28E+00 3.04E+00 4.07E+00 3.37E+00 3.81E+00 1.45E+00 3.98E+00 2.80E+00 3.01E+00 3.22E+00 1.98E+00 2.18E+00 1.49E+00 2.91E+00 1.70E+00 3.57E+00 2.39E+00 6.42E+00 2.76E+00 2.66E+00 1.10E+00 5.82E-01 7.44E-01 1.54E+00 9.07E-02 2.70E+00 7.66E-01 1.16E+00 1.13E+00 1.01E+00 1.21E+00 1.95E+00 5.27E-01 1.13E-01 2.66E-02 1.60E-02 1.66E-02 2.82E-01 1.34E+00 8.81E-01 3.10E-02 1.83E-01 5.02E-01 4.79E-01 6.01E-01 1.70E-01 5.14E-02 6.42E-02 5.68E-01 3.74E-02 5.60E-01 1.04E+00 1.77E-01 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name m/z KEGG Pubchem SID Pubchem CID Octopamine 289.95 C04227 6898 440266 Citrulline 311.2 C00327 3621 9750 Dopamine 289.2 C03758 6517 681 METABOLITES_END #END