{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000070","ANALYSIS_ID":"AN000109","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and mice","PROJECT_TYPE":"Effects and role of obesity on the severity of influenza","PROJECT_SUMMARY":"During the 2009 H1N1 influenza pandemic outbreak, obese individuals were to be at greater risk for morbidity and mortality from pandemic infection. the mechanisms contributing to greater influenza severity in infected obese remain unclear. Given that one in ten individuals is obese, and worldwide outbreaks are a consistent public health burden, garnering a better of the pathways and mechanisms contributing to greater influenza severity in obese is essential for limiting influenza infection mortality in this at-risk Closely paralleling pH1N1 infection outcome in humans, obese mice exhibit morbidity and mortality following pH1N1 infection. In mice, obesity impairs the of natural killer cells, dendritic cells, macrophage, B cells and memory T Further, several analyses of lung antiviral responses revealed that obese mice greater lung damage, lung immune cell infiltration and impaired lung healing infection. Nevertheless, it remains unclear how altered immune cell function to greater lung damage and increased infection severity in obese mice. will be used to dissect the metabolic consequences of obesity on the immune to pH1N1 infection. We will compare metabolic profiles of lung-specific and samples from uninfected and infected lean and obese mice during early and late of influenza immunity.","INSTITUTE":"University of North Carolina at Chapel Hill","DEPARTMENT":"Department of Nutrition","LAST_NAME":"Beck","FIRST_NAME":"Melinda","ADDRESS":"2303 MHRB, CB #7461, UNC, Chapel Hill NC 27599","EMAIL":"melinda_beck@unc.edu","PHONE":"919-966-6809"},

"STUDY":{"STUDY_TITLE":"Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and mice (via tissue)","STUDY_TYPE":"Metabolomics analysis on the effec of genetically-derived obesity on the immune to pH1N1 infection","STUDY_SUMMARY":"-","INSTITUTE":"RTI International","DEPARTMENT":"Systems and Translational Sciences (STS)","LABORATORY":"RTI Regional Comprehensive Metabolomics Resource Core (RTI RCMRC)","LAST_NAME":"Sumner","FIRST_NAME":"Susan","ADDRESS":"3040 East Cornwallis Road, Research Triangle Park, NC 27709, USA","EMAIL":"ssumner@rti.org","PHONE":"919-541-7479","NUM_GROUPS":"4","TOTAL_SUBJECTS":"22"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","AGE_OR_AGE_RANGE":"approx. 15-16 weeks","GENDER":"male and female","ANIMAL_ANIMAL_SUPPLIER":"The Jackson Laboratory","ANIMAL_FEED":"high fat, low fat or normal chow","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LPALL0001",
"Factors":{"Phenotype":"--","Infection Status":"--","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LPALL0002",
"Factors":{"Phenotype":"--","Infection Status":"--","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LPALL0003",
"Factors":{"Phenotype":"--","Infection Status":"--","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLI8_67",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLI8_68",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLI8_92",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLI8_65",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLI8_86",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLI8_94",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLPD8001",
"Factors":{"Phenotype":"lean","Infection Status":"infected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLPD8002",
"Factors":{"Phenotype":"lean","Infection Status":"infected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLPD8003",
"Factors":{"Phenotype":"lean","Infection Status":"infected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLPCTRL001",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLPCTRL002",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLPCTRL003",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLC_40",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLC_56",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLC_57",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLC_36",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_LLC_47",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLC_51",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLI8_74",
"Factors":{"Phenotype":"obese","Infection Status":"D8 infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLI8_75",
"Factors":{"Phenotype":"obese","Infection Status":"D8 infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLI8_91",
"Factors":{"Phenotype":"obese","Infection Status":"D8 infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLI8_109",
"Factors":{"Phenotype":"obese","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLI8_88",
"Factors":{"Phenotype":"obese","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLPD8001",
"Factors":{"Phenotype":"obese","Infection Status":"infected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLPD8002",
"Factors":{"Phenotype":"obese","Infection Status":"infected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLPD8003",
"Factors":{"Phenotype":"obese","Infection Status":"infected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLPCTRL001",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLPCTRL002",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLPCTRL003",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLC_41",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLC_44",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLC_63",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLC_35",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"SD_02242014_RP_OLC_53",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"m"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Tissue","STORAGE_CONDITIONS":"-80"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 per 1 mg of tissue using washed ceramic beads. Extraction was performed using Samples were dried down and reconstituted in 100uL of 95:5 water:methanol.","SAMPLEPREP_PROTOCOL_FILENAME":"RTI_BECK_Lung-Genetic_RP_Metabolomics_Procedure.docx","PROCESSING_METHOD":"Homogenization","EXTRACTION_METHOD":"Acetonitrile","EXTRACT_STORAGE":"-80 C","SAMPLE_RESUSPENSION":"95:5 Water:Methanol","SAMPLE_SPIKING":"L-Tryptophan-d5","ORGAN":"Lung"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)","COLUMN_PRESSURE":"6000-10000","COLUMN_TEMPERATURE":"50 C","FLOW_GRADIENT":"Time(min) Flow Rate %A %B Curve   1. Initial 0.400 100.0 0.0   2. 0.400 100.0 0.0 6   3. 16.00 0.400 0.0 100.0 6   4. 20.00 0.400 0.0 100.0 5. 22.00 0.400 100.0 0.0 6","FLOW_RATE":"0.4 mL/min","INJECTION_TEMPERATURE":"8 C","INTERNAL_STANDARD":"L-Tryptophan-d5","SOLVENT_A":"Water w/ 0.1% formic acid","SOLVENT_B":"Methanol w/ 0.1% formic acid","ANALYTICAL_TIME":"22 min","WEAK_WASH_SOLVENT_NAME":"5%MeOH","WEAK_WASH_VOLUME":"1000 uL","STRONG_WASH_SOLVENT_NAME":"80%MeOH","STRONG_WASH_VOLUME":"1000 uL","TARGET_SAMPLE_TEMPERATURE":"8 C","SAMPLE_LOOP_SIZE":"10 uL","SAMPLE_SYRINGE_SIZE":"100 uL","RANDOMIZATION_ORDER":"Yes"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"RTI","ACQUISITION_DATE":"41656","SOFTWARE_VERSION":"MassLynx 4.1","OPERATOR_NAME":"Suraj Dhungana, Darya Cheng","DETECTOR_TYPE":"TOF (+ive mode)"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Waters Synapt G2 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","CAPILLARY_TEMPERATURE":"110 C","CAPILLARY_VOLTAGE":"3.2 kV","COLLISION_ENERGY":"4","FRAGMENTATION_METHOD":"CID","HELIUM_FLOW":"180","IONIZATION":"ES+","MASS_ACCURACY":"10 ppm","SOURCE_TEMPERATURE":"110 C","DATAFORMAT":"Continuum","DESOLVATION_GAS_FLOW":"400 L/Hr","DESOLVATION_TEMPERATURE":"400 C","RESOLUTION_SETTING":"18000","SCAN_RANGE_MOVERZ":"50-1000 m/s","SCANNING_CYCLE":"1 s","TUBE_LENS_VOLTAGE":"75"},

"MS_METABOLITE_DATA":{
"Units":"Normalized abundance",

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