{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000071","ANALYSIS_ID":"AN000111","VERSION":"1","CREATED_ON":"03-03-2022"},

"PROJECT":{"PROJECT_TITLE":"Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice","PROJECT_TYPE":"Effects and role of obesity on the severity of influenza","PROJECT_SUMMARY":"During the 2009 H1N1 influenza pandemic outbreak, obese individuals were reported to be at greater risk for morbidity and mortality from pandemic infection. However, the mechanisms contributing to greater influenza severity in infected obese individuals remain unclear. Given that one in ten individuals is obese, and worldwide influenza outbreaks are a consistent public health burden, garnering a better understanding of the pathways and mechanisms contributing to greater influenza severity in the obese is essential for limiting influenza infection mortality in this at-risk population. Closely paralleling pH1N1 infection outcome in humans, obese mice exhibit increased morbidity and mortality following pH1N1 infection. In mice, obesity impairs the function of natural killer cells, dendritic cells, macrophage, B cells and memory T cells. Further, several analyses of lung antiviral responses revealed that obese mice have greater lung damage, lung immune cell infiltration and impaired lung healing after infection. Nevertheless, it remains unclear how altered immune cell function contributes to greater lung damage and increased infection severity in obese mice. Metabolomics will be used to dissect the metabolic consequences of obesity on the immune response to pH1N1 infection. We will compare metabolic profiles of lung-specific and peripheral samples from uninfected and infected lean and obese mice during early and late phases of influenza immunity.","INSTITUTE":"University of North Carolina at Chapel Hill","DEPARTMENT":"Department of Nutrition","LAST_NAME":"Beck","FIRST_NAME":"Melinda","ADDRESS":"2303 MHRB, CB #7461, UNC, Chapel Hill NC 27599","EMAIL":"melinda_beck@unc.edu","PHONE":"919-966-6809","DOI":"http://dx.doi.org/10.21228/M8QG6H"},

"STUDY":{"STUDY_TITLE":"Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice (via Urine)","INSTITUTE":"University of North Carolina","DEPARTMENT":"Systems and Translational Sciences","LABORATORY":"Sumner Lab","LAST_NAME":"Sumner","FIRST_NAME":"Susan","ADDRESS":"Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081","EMAIL":"susan_sumner@unc.edu","PHONE":"704-250-5066","SUBMIT_DATE":"2014-06-14"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","AGE_OR_AGE_RANGE":"approx. 15-16 weeks","GENDER":"male and female","ANIMAL_ANIMAL_SUPPLIER":"The Jackson Laboratory","ANIMAL_FEED":"high fat, low fat or normal chow","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"UPALL0001",
"Factors":{"Phenotype":"--","Infection Status":"--","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"UPALL0002",
"Factors":{"Phenotype":"--","Infection Status":"--","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"UPALL0003",
"Factors":{"Phenotype":"--","Infection Status":"--","Sex":"--"}
},
{
"Subject ID":"-",
"Sample ID":"LUI2_67",
"Factors":{"Phenotype":"lean","Infection Status":"D2 Infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"LUI2_68",
"Factors":{"Phenotype":"lean","Infection Status":"D2 Infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"LUI2_92",
"Factors":{"Phenotype":"lean","Infection Status":"D2 Infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"65",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"86",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"94",
"Factors":{"Phenotype":"lean","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"LUC_67",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"LUC_68",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"LUC_92",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"36",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"47",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"51",
"Factors":{"Phenotype":"lean","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"OUI2_74",
"Factors":{"Phenotype":"obese","Infection Status":"D2 Infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"OUI2_75",
"Factors":{"Phenotype":"obese","Infection Status":"D2 Infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"OUI2_91",
"Factors":{"Phenotype":"obese","Infection Status":"D2 Infected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"109",
"Factors":{"Phenotype":"obese","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"88",
"Factors":{"Phenotype":"obese","Infection Status":"D8 infected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"OUC_74",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"OUC_75",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"OUC_91",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"f"}
},
{
"Subject ID":"-",
"Sample ID":"35",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"m"}
},
{
"Subject ID":"-",
"Sample ID":"53",
"Factors":{"Phenotype":"obese","Infection Status":"uninfected","Sex":"m"}
}
],
"COLLECTION":{"SAMPLE_TYPE":"Tissue","STORAGE_CONDITIONS":"-80"},

"TREATMENT":[],

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down  and reconstituted in 100uL of 95:5 water:methanol.","SAMPLEPREP_PROTOCOL_FILENAME":"RTI_BECK_Urine-Genetic_HILIC_Metabolomics_Procedure.docx","PROCESSING_METHOD":"Homogenization","EXTRACTION_METHOD":"Acetonitrile","EXTRACT_STORAGE":"-80 C","SAMPLE_RESUSPENSION":"95:5 Water:Methanol","SAMPLE_SPIKING":"L-Tryptophan-d5","ORGAN":"Lung"},

"CHROMATOGRAPHY":{"INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)","COLUMN_PRESSURE":"6000-10000","COLUMN_TEMPERATURE":"40 C","FLOW_GRADIENT":"Time(min) Flow Rate %A %B: 1. Initial 0.400 99.0 1.0; 2. 1.00 0.400 99.0 1.0; 3. 12.00 0.400 1.0 99.0; 4. 14.00 0.400 1.0 99.0; 5. 15.00 0.400 99.0 1.0; 6. 19.00 0.400 99.0 1.0","FLOW_RATE":"0.4 mL/min","INJECTION_TEMPERATURE":"8 C","INTERNAL_STANDARD":"L-Tryptophan-d5","SOLVENT_A":"10mM ammonium acetate in 95/5 acetonitrile/water with 0.1% formic acid","SOLVENT_B":"10mM ammonium acetate in 50/50 acetonitrile/water with 0.1% formic acid","ANALYTICAL_TIME":"19 min","WEAK_WASH_SOLVENT_NAME":"5%MeOH","WEAK_WASH_VOLUME":"1000 uL","STRONG_WASH_SOLVENT_NAME":"80%MeOH","STRONG_WASH_VOLUME":"1000 uL","TARGET_SAMPLE_TEMPERATURE":"8 C","SAMPLE_LOOP_SIZE":"10 uL","SAMPLE_SYRINGE_SIZE":"100 uL","RANDOMIZATION_ORDER":"Yes","CHROMATOGRAPHY_TYPE":"HILIC"},

"ANALYSIS":{"LABORATORY_NAME":"RTI","ANALYSIS_TYPE":"MS","ACQUISITION_DATE":"41656","SOFTWARE_VERSION":"MassLynx 4.1","OPERATOR_NAME":"Suraj Dhungana, Darya Cheng","DETECTOR_TYPE":"TOF (positive)"},

"MS":{"INSTRUMENT_NAME":"Waters Synapt G2 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","CAPILLARY_TEMPERATURE":"110 C","CAPILLARY_VOLTAGE":"3.2 kV","COLLISION_ENERGY":"4","FRAGMENTATION_METHOD":"CID","HELIUM_FLOW":"180","IONIZATION":"ES+","MASS_ACCURACY":"10 ppm","SOURCE_TEMPERATURE":"110 C","DATAFORMAT":"Continuum","DESOLVATION_GAS_FLOW":"400 L/Hr","DESOLVATION_TEMPERATURE":"400 C","RESOLUTION_SETTING":"18000","SCAN_RANGE_MOVERZ":"50-1000 m/s","SCANNING_CYCLE":"1 s","TUBE_LENS_VOLTAGE":"75","MS_RESULTS_FILE":"ST000071_AN000111_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes"}

}