{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000101","ANALYSIS_ID":"AN000168","VERSION":"1","CREATED_ON":"10-02-2015"},

"PROJECT":{"PROJECT_TITLE":"13C Metabolomics: Applications at Natural Abundance","INSTITUTE":"University of Florida","DEPARTMENT":"Department of Biochemistry and Molecular Biology","LABORATORY":"Edison","LAST_NAME":"Edison","FIRST_NAME":"Arthur","ADDRESS":"R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245","EMAIL":"aedison@ufl.edu","PHONE":"352-392-4535","PROJECT_SUMMARY":"-"},

"STUDY":{"STUDY_TITLE":"NMR analysis of Synthetic Mixture Analysis","STUDY_TYPE":"Synthetic","STUDY_SUMMARY":"Two groups of mixtures with 5 replicates each were each made using 20 synthetic metabolites. Some metabolites were at equal concentrations in both groups, and 10 metabolites differed between groups with half higher in group A, and half higher in group B.","INSTITUTE":"University of Florida","DEPARTMENT":"Department of Biochemistry and Molecular Biology /SECIM","LABORATORY":"Arthur Edison","LAST_NAME":"Arthur","FIRST_NAME":"Edison","ADDRESS":"R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245","EMAIL":"aedison@ufl.edu","NUM_GROUPS":"2","TOTAL_SUBJECTS":"10","PHONE":"-"},

"SUBJECT":{"SUBJECT_TYPE":"Synthetic Sample","SUBJECT_SPECIES":"N/A"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Mixture B1",
"Factors":{"Mixture Concentration (mM)":"(Arg,Nicotinamide,Lys,Trp,Carnitine)-High ( Met,Pro,Glu,Glucose,Ile)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture B2",
"Factors":{"Mixture Concentration (mM)":"(Arg,Nicotinamide,Lys,Trp,Carnitine)-High ( Met,Pro,Glu,Glucose,Ile)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture B3",
"Factors":{"Mixture Concentration (mM)":"(Arg,Nicotinamide,Lys,Trp,Carnitine)-High ( Met,Pro,Glu,Glucose,Ile)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture B4",
"Factors":{"Mixture Concentration (mM)":"(Arg,Nicotinamide,Lys,Trp,Carnitine)-High ( Met,Pro,Glu,Glucose,Ile)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture B5",
"Factors":{"Mixture Concentration (mM)":"(Arg,Nicotinamide,Lys,Trp,Carnitine)-High ( Met,Pro,Glu,Glucose,Ile)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture A1",
"Factors":{"Mixture Concentration (mM)":"( Met,Pro,Glu,Glucose,Ile)-High (Arg,Nicotinamide,Lys,Trp,Carnitine)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture A2",
"Factors":{"Mixture Concentration (mM)":"( Met,Pro,Glu,Glucose,Ile)-High (Arg,Nicotinamide,Lys,Trp,Carnitine)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture A3",
"Factors":{"Mixture Concentration (mM)":"( Met,Pro,Glu,Glucose,Ile)-High (Arg,Nicotinamide,Lys,Trp,Carnitine)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture A4",
"Factors":{"Mixture Concentration (mM)":"( Met,Pro,Glu,Glucose,Ile)-High (Arg,Nicotinamide,Lys,Trp,Carnitine)-Low"}
},
{
"Subject ID":"-",
"Sample ID":"Mixture A5",
"Factors":{"Mixture Concentration (mM)":"( Met,Pro,Glu,Glucose,Ile)-High (Arg,Nicotinamide,Lys,Trp,Carnitine)-Low"}
}
],
"COLLECTION":{"SAMPLE_TYPE":"Synthetic Mixture","VOLUMEORAMOUNT_COLLECTED":"50 microliters","STORAGE_CONDITIONS":"-80 degree Celsius","COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Two groups of mixtures with 5 replicates each were each made using 20 synthetic metabolites ranging in concentration from 1 to 5 mM. The first ten metabolites were at equal concentrations in both groups, and the remaining 10 metabolites differed between groups with half higher in group A, and half higher in group B. Samples were brought to 50 microliters using 99% D2O and 40 microliters were pipetted into a 1.5 mm tube.","SAMPLEPREP_PROTOCOL_FILENAME":"SyntheticMix_Concentrations.pdf","SAMPLEPREP_PROTOCOL_COMMENTS":"10 metabolites were of similar concentration between the two groups."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"LABORATORY_NAME":"Edison","ANALYSIS_TYPE":"NMR","SOFTWARE_VERSION":"VNMRJ","OPERATOR_NAME":"Chaevien Clendinen","DATA_FORMAT":"Varian"},

"NM":{"INSTRUMENT_TYPE":"Varian","NMR_EXPERIMENT_TYPE":"1D 1H","SPECTROMETER_FREQUENCY":"600 MHz","NMR_PROBE":"1.5 mm HTS cryoprobe","NMR_SOLVENT":"D2O","NMR_TUBE_SIZE":"1.5 mm","SHIMMING_METHOD":"Manual","PULSE_SEQUENCE":"1D 1H","WATER_SUPPRESSION":"Presaturation","PULSE_WIDTH":"45","RECEIVER_GAIN":"28","OFFSET_FREQUENCY":"4.77","CHEMICAL_SHIFT_REF_CPD":"TSP","TEMPERATURE":"22 degree Celsius","NUMBER_OF_SCANS":"64","DUMMY_SCANS":"16","ACQUISITION_TIME":"2.0s","RELAXATION_DELAY":"2s","SPECTRAL_WIDTH":"12 ppm","NUM_DATA_POINTS_ACQUIRED":"32768","REAL_DATA_POINTS":"16384","ZERO_FILLING":"2x","APODIZATION":"Cosine squared","INSTRUMENT_NAME":"Varian"},

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