{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000111","ANALYSIS_ID":"AN000186","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"RCSIRM Outreach and Education 2014 Metabolomics Workshop","PROJECT_SUMMARY":"Demonstrating the utility and practicality of Stable Isotope Enriched Cell to investigation of cell metabolism","INSTITUTE":"University of Kentucky","DEPARTMENT":"Toxicology","LAST_NAME":"Fan","FIRST_NAME":"Teresa","ADDRESS":"-","EMAIL":"twmfan@gmail.com","PHONE":"-"},

"STUDY":{"STUDY_TITLE":"Study of biological variation in PC9 cell culture","STUDY_TYPE":"unlabeled","STUDY_SUMMARY":"Use extra plates seeded for workshop to investigate biological variation with tracer and no treatment","INSTITUTE":"University of Kentucky","DEPARTMENT":"Toxicology","LAST_NAME":"Fan","FIRST_NAME":"Teresa","ADDRESS":"-","EMAIL":"twmfan@gmail.com","PHONE":"-"},

"SUBJECT":{"SUBJECT_TYPE":"cell culture","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENOTYPE_STRAIN":"PC9","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"PC9_CTL_unL_19_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_19_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09988","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_20_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_20_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09846","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_21_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_21_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09977","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_22_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_22_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09951","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_23_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_23_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09891","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_24_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_24_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.1001","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_25_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_25_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.10012","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_26_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_26_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09831","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_27_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_27_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.10096","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_28_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_28_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.10084","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_29_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_29_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09971","wet_weight%units":"g"}
},
{
"Subject ID":"PC9_CTL_unL_30_29Jul14-plate",
"Sample ID":"PC9_CTL_unL_30_29Jul14-plate",
"Factors":{"timepoint":"24 hrs"},
"Additional sample data":{"time_point":"24","time_point%units":"h","type":"","wet_weight":"0.09913","wet_weight%units":"g"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Plate media | Cell quench collection","COLLECTION_PROTOCOL_FILENAME":"2014-07-29_WS_Fan_Quench_cell_tissue.pdf","COLLECTION_PROTOCOL_COMMENTS":"media collection from plate | Collection and quench of cells in tissue culture"},

"TREATMENT":{"TREATMENT_SUMMARY":"-","TREATMENT_PROTOCOL_FILENAME":"2014-07-29_WS_Fan_Cell_Tracer_Ex.pdf","TREATMENT_PROTOCOL_COMMENTS":"cell culture in unlabeled media for 24h"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Protein extraction | Polar extraction","SAMPLEPREP_PROTOCOL_FILENAME":"2014-07-29_WS_Fan_Prep_Polar.pdf | 2014-07-29_WS_Fan_Prot_Quant.pdf","SAMPLEPREP_PROTOCOL_COMMENTS":"Preparation of polar samples for GCMS and NMR. | Protein extraction and"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"RCSIRM"},

"MS":{"INSTRUMENT_NAME":"Thermo ITQ","INSTRUMENT_TYPE":"GC-ITQ","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"GCMS Analytical Experiment with detection of compounds by comparison to Derivitization is done with MTBSTFA"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"a-glycerol-3P_1-13C0","PC9_CTL_unL_19_29Jul14-plate":"0.3610","PC9_CTL_unL_20_29Jul14-plate":"0.6733","PC9_CTL_unL_21_29Jul14-plate":"0.5312","PC9_CTL_unL_22_29Jul14-plate":"0.7284","PC9_CTL_unL_23_29Jul14-plate":"0.7590","PC9_CTL_unL_24_29Jul14-plate":"0.6182","PC9_CTL_unL_25_29Jul14-plate":"0.6199","PC9_CTL_unL_26_29Jul14-plate":"0.7163","PC9_CTL_unL_27_29Jul14-plate":"0.5145","PC9_CTL_unL_28_29Jul14-plate":"0.6334","PC9_CTL_unL_29_29Jul14-plate":"0.7003","PC9_CTL_unL_30_29Jul14-plate":"0.5832"},{"Metabolite":"a-glycerol-3P_1-13C1","PC9_CTL_unL_19_29Jul14-plate":"","PC9_CTL_unL_20_29Jul14-plate":"","PC9_CTL_unL_21_29Jul14-plate":"0.0165","PC9_CTL_unL_22_29Jul14-plate":"","PC9_CTL_unL_23_29Jul14-plate":"0.0559","PC9_CTL_unL_24_29Jul14-plate":"","PC9_CTL_unL_25_29Jul14-plate":"0.0102","PC9_CTL_unL_26_29Jul14-plate":"","PC9_CTL_unL_27_29Jul14-plate":"0.0449","PC9_CTL_unL_28_29Jul14-plate":"","PC9_CTL_unL_29_29Jul14-plate":"","PC9_CTL_unL_30_29Jul14-plate":""},{"Metabolite":"a-glycerol-3P_1-13C2","PC9_CTL_unL_19_29Jul14-plate":"","PC9_CTL_unL_20_29Jul14-plate":"0.0099","PC9_CTL_unL_21_29Jul14-plate":"","PC9_CTL_unL_22_29Jul14-plate":"0.0111","PC9_CTL_unL_23_29Jul14-plate":"","PC9_CTL_unL_24_29Jul14-plate":"","PC9_CTL_unL_25_29Jul14-plate":"0.0255","PC9_CTL_unL_26_29Jul14-plate":"0.0050","PC9_CTL_unL_27_29Jul14-plate":"","PC9_CTL_unL_28_29Jul14-plate":"","PC9_CTL_unL_29_29Jul14-plate":"","PC9_CTL_unL_30_29Jul14-plate":""},{"Metabolite":"a-glycerol-3P_1-13C3","PC9_CTL_unL_19_29Jul14-plate":"","PC9_CTL_unL_20_29Jul14-plate":"","PC9_CTL_unL_21_29Jul14-plate":"","PC9_CTL_unL_22_29Jul14-plate":"","PC9_CTL_unL_23_29Jul14-plate":"","PC9_CTL_unL_24_29Jul14-plate":"","PC9_CTL_unL_25_29Jul14-plate":"","PC9_CTL_unL_26_29Jul14-plate":"","PC9_CTL_unL_27_29Jul14-plate":"","PC9_CTL_unL_28_29Jul14-plate":"","PC9_CTL_unL_29_29Jul14-plate":"","PC9_CTL_unL_30_29Jul14-plate":"0.0004"},{"Metabolite":"a-glycerol-3P_1-ALL","PC9_CTL_unL_19_29Jul14-plate":"0.3610","PC9_CTL_unL_20_29Jul14-plate":"0.6832","PC9_CTL_unL_21_29Jul14-plate":"0.5477","PC9_CTL_unL_22_29Jul14-plate":"0.7395","PC9_CTL_unL_23_29Jul14-plate":"0.8148","PC9_CTL_unL_24_29Jul14-plate":"0.6182","PC9_CTL_unL_25_29Jul14-plate":"0.6555","PC9_CTL_unL_26_29Jul14-plate":"0.7213","PC9_CTL_unL_27_29Jul14-plate":"0.5594","PC9_CTL_unL_28_29Jul14-plate":"0.6334","PC9_CTL_unL_29_29Jul14-plate":"0.7003","PC9_CTL_unL_30_29Jul14-plate":"0.5836"},{"Metabolite":"a-glycerol-3P_2-13C0","PC9_CTL_unL_19_29Jul14-plate":"0.2881","PC9_CTL_unL_20_29Jul14-plate":"0.5813","PC9_CTL_unL_21_29Jul14-plate":"0.5230","PC9_CTL_unL_22_29Jul14-plate":"0.6013","PC9_CTL_unL_23_29Jul14-plate":"0.6844","PC9_CTL_unL_24_29Jul14-plate":"0.4997","PC9_CTL_unL_25_29Jul14-plate":"0.5768","PC9_CTL_unL_26_29Jul14-plate":"0.5555","PC9_CTL_unL_27_29Jul14-plate":"0.4692","PC9_CTL_unL_28_29Jul14-plate":"0.5849","PC9_CTL_unL_29_29Jul14-plate":"0.6017","PC9_CTL_unL_30_29Jul14-plate":"0.5204"},{"Metabolite":"a-glycerol-3P_2-13C1","PC9_CTL_unL_19_29Jul14-plate":"","PC9_CTL_unL_20_29Jul14-plate":"","PC9_CTL_unL_21_29Jul14-plate":"","PC9_CTL_unL_22_29Jul14-plate":"","PC9_CTL_unL_23_29Jul14-plate":"","PC9_CTL_unL_24_29Jul14-plate":"","PC9_CTL_unL_25_29Jul14-plate":"","PC9_CTL_unL_26_29Jul14-plate":"","PC9_CTL_unL_27_29Jul14-plate":"","PC9_CTL_unL_28_29Jul14-plate":"","PC9_CTL_unL_29_29Jul14-plate":"","PC9_CTL_unL_30_29Jul14-plate":""},{"Metabolite":"a-glycerol-3P_2-13C2","PC9_CTL_unL_19_29Jul14-plate":"","PC9_CTL_unL_20_29Jul14-plate":"","PC9_CTL_unL_21_29Jul14-plate":"","PC9_CTL_unL_22_29Jul14-plate":"","PC9_CTL_unL_23_29Jul14-plate":"","PC9_CTL_unL_24_29Jul14-plate":"","PC9_CTL_unL_25_29Jul14-plate":"","PC9_CTL_unL_26_29Jul14-plate":"","PC9_CTL_unL_27_29Jul14-plate":"","PC9_CTL_unL_28_29Jul14-plate":"","PC9_CTL_unL_29_29Jul14-plate":"","PC9_CTL_unL_30_29Jul14-plate":""},{"Metabolite":"a-glycerol-3P_2-13C3","PC9_CTL_unL_19_29Jul14-plate":"","PC9_CTL_unL_20_29Jul14-plate":"","PC9_CTL_unL_21_29Jul14-plate":"","PC9_CTL_unL_22_29Jul14-plate":"","PC9_CTL_unL_23_29Jul14-plate":"","PC9_CTL_unL_24_29Jul14-plate":"","PC9_CTL_unL_25_29Jul14-plate":"","PC9_CTL_unL_26_29Jul14-plate":"","PC9_CTL_unL_27_29Jul14-plate":"","PC9_CTL_unL_28_29Jul14-plate":"","PC9_CTL_unL_29_29Jul14-plate":"","PC9_CTL_unL_30_29Jul14-plate":""},{"Metabolite":"a-glycerol-3P_2-ALL","PC9_CTL_unL_19_29Jul14-plate":"0.2881","PC9_CTL_unL_20_29Jul14-plate":"0.5813","PC9_CTL_unL_21_29Jul14-plate":"0.5230","PC9_CTL_unL_22_29Jul14-plate":"0.6013","PC9_CTL_unL_23_29Jul14-plate":"0.6844","PC9_CTL_unL_24_29Jul14-plate":"0.4997","PC9_CTL_unL_25_29Jul14-plate":"0.5768","PC9_CTL_unL_26_29Jul14-plate":"0.5555","PC9_CTL_unL_27_29Jul14-plate":"0.4692","PC9_CTL_unL_28_29Jul14-plate":"0.5849","PC9_CTL_unL_29_29Jul14-plate":"0.6017","PC9_CTL_unL_30_29Jul14-plate":"0.5204"},{"Metabolite":"a-ketoglutarate-13C0","PC9_CTL_unL_19_29Jul14-plate":"0.0154","PC9_CTL_unL_20_29Jul14-plate":"0.0305","PC9_CTL_unL_21_29Jul14-plate":"0.0280","PC9_CTL_unL_22_29Jul14-plate":"0.0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