{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000211","ANALYSIS_ID":"AN000313","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Graft-Versus-Host Disease","PROJECT_SUMMARY":"T cell metabolism during graft-versus-host disease","INSTITUTE":"University of Michigan","DEPARTMENT":"Pediatrics","LABORATORY":"Ferrara lab","LAST_NAME":"Byersdorfer","FIRST_NAME":"Craig","ADDRESS":"Ann Arbor, MI","EMAIL":"craigbye@med.umich.edu","PHONE":"734-000-0000"},

"STUDY":{"STUDY_TITLE":"T cell metabolism during graft-versus-host disease (CAB 307)-PART II","STUDY_SUMMARY":"T cells were injected into mis-matched animals undergoing bone marrow On day 7 after BMT, cells were receovered and CD8 T cells were purified to > purity over magnetic column. Cells were washed once in PBS, then once in chloride, residual volume was removed and cell pellets were flash frozein in and stored at -80C","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@umich.edu","PHONE":"-","NUM_GROUPS":"2","TOTAL_SUBJECTS":"7"},

"SUBJECT":{"SUBJECT_TYPE":"-","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0004282",
"Sample ID":"S00015097",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"SU0004282",
"Sample ID":"S00015098",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"SU0004282",
"Sample ID":"S00015099",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"SU0004282",
"Sample ID":"S00015100",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"SU0004281",
"Sample ID":"S00015094",
"Factors":{"Treatment":"GVHD"}
},
{
"Subject ID":"SU0004281",
"Sample ID":"S00015095",
"Factors":{"Treatment":"GVHD"}
},
{
"Subject ID":"SU0004281",
"Sample ID":"S00015096",
"Factors":{"Treatment":"GVHD"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"Acyl-carnitines_analysis_protocol-CR-MK-20150528.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1200","COLUMN_NAME":"Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)","METHODS_ID":"AQM050","METHODS_FILENAME":"QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"MRC2 (University of Michigan)","ACQUISITION_PARAMETERS_FILE":"QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip","PROCESSING_PARAMETERS_FILE":"EX00317-acyl-carnitines-quant.m.zip"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6490 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"pmol/100 µg protein",

"Data":[{"Metabolite":"3-hydroxylauroylcarnitine","S00015097":"0.0000","S00015098":"0.0000","S00015099":"0.0020","S00015100":"0.0010","S00015094":"0.0000","S00015095":"0.0000","S00015096":"0.0002"},{"Metabolite":"3-Hydroxylinoleoylcarnitine","S00015097":"0.0425","S00015098":"0.0599","S00015099":"0.0820","S00015100":"0.0425","S00015094":"0.1207","S00015095":"0.1464","S00015096":"0.1339"},{"Metabolite":"3-hydroxymyristoylcarnitine","S00015097":"0.0005","S00015098":"0.0030","S00015099":"0.0092","S00015100":"0.0069","S00015094":"0.0016","S00015095":"0.0041","S00015096":"0.0058"},{"Metabolite":"3-Hydroxypalmitoylcarnitine","S00015097":"0.0059","S00015098":"0.0102","S00015099":"0.0146","S00015100":"0.0110","S00015094":"0.0113","S00015095":"0.0116","S00015096":"0.0140"},{"Metabolite":"Acetylcarnitine","S00015097":"10.3319","S00015098":"15.0108","S00015099":"12.6435","S00015100":"7.7084","S00015094":"9.6781","S00015095":"9.1973","S00015096":"12.5030"},{"Metabolite":"arachidonoylcarnitine","S00015097":"0.0102","S00015098":"0.0141","S00015099":"0.0309","S00015100":"0.0149","S00015094":"0.0248","S00015095":"0.0208","S00015096":"0.0247"},{"Metabolite":"butyrylcarnitine","S00015097":"0.2897","S00015098":"0.6425","S00015099":"0.7617","S00015100":"0.4359","S00015094":"0.2782","S00015095":"0.2747","S00015096":"0.3535"},{"Metabolite":"Decanoylcarnitine","S00015097":"0.0022","S00015098":"0.0023","S00015099":"0.0457","S00015100":"0.0236","S00015094":"0.0046","S00015095":"0.0003","S00015096":"0.0000"},{"Metabolite":"decenoylcarnitine","S00015097":"0.0000","S00015098":"0.0000","S00015099":"0.0118","S00015100":"0.0049","S00015094":"0.0000","S00015095":"0.0000","S00015096":"0.0000"},{"Metabolite":"Dodecenoylcarnitine","S00015097":"0.0291","S00015098":"0.0250","S00015099":"0.2537","S00015100":"0.0925","S00015094":"0.0127","S00015095":"0.0053","S00015096":"0.0160"},{"Metabolite":"Glutarylcarnitine","S00015097":"0.1422","S00015098":"0.1097","S00015099":"0.1356","S00015100":"0.0819","S00015094":"0.6098","S00015095":"0.5146","S00015096":"0.6036"},{"Metabolite":"Hexanoylcarnitine","S00015097":"0.0656","S00015098":"0.1100","S00015099":"0.2596","S00015100":"0.1125","S00015094":"0.0317","S00015095":"0.0303","S00015096":"0.0338"},{"Metabolite":"icosadienoylcarnitine","S00015097":"0.0106","S00015098":"0.0079","S00015099":"0.0228","S00015100":"0.0089","S00015094":"0.0184","S00015095":"0.0140","S00015096":"0.0149"},{"Metabolite":"Icosanoylcarnitine","S00015097":"0.0000","S00015098":"0.0000","S00015099":"0.0000","S00015100":"0.0000","S00015094":"0.0000","S00015095":"0.0000","S00015096":"0.0000"},{"Metabolite":"icosatrienoylcarnitine","S00015097":"0.1765","S00015098":"0.2062","S00015099":"0.1744","S00015100":"0.1114","S00015094":"0.5803","S00015095":"0.5892","S00015096":"0.6639"},{"Metabolite":"icoseneoylcarnitine","S00015097":"0.0092","S00015098":"0.0079","S00015099":"0.0084","S00015100":"0.0062","S00015094":"0.0121","S00015095":"0.0165","S00015096":"0.0192"},{"Metabolite":"isovalerylcarnitine","S00015097":"0.0851","S00015098":"0.0751","S00015099":"0.1052","S00015100":"0.0646","S00015094":"0.0612","S00015095":"0.0567","S00015096":"0.0631"},{"Metabolite":"lauroylcarnitine","S00015097":"0.0029","S00015098":"0.0004","S00015099":"0.0032","S00015100":"0.0013","S00015094":"0.0000","S00015095":"0.0007","S00015096":"0.0000"},{"Metabolite":"L-Carnitine","S00015097":"30.5671","S00015098":"22.0590","S00015099":"24.4200","S00015100":"10.9331","S00015094":"40.0053","S00015095":"31.7410","S00015096":"34.7352"},{"Metabolite":"Linoelaidyl carnitine","S00015097":"0.0077","S00015098":"0.0055","S00015099":"0.0072","S00015100":"0.0046","S00015094":"0.0291","S00015095":"0.0210","S00015096":"0.0197"},{"Metabolite":"myristoylcarnitine","S00015097":"0.0407","S00015098":"0.0157","S00015099":"0.0586","S00015100":"0.0263","S00015094":"0.0281","S00015095":"0.0266","S00015096":"0.0181"},{"Metabolite":"octanoylcarnitine","S00015097":"0.0298","S00015098":"0.0363","S00015099":"0.0927","S00015100":"0.0366","S00015094":"0.0164","S00015095":"0.0132","S00015096":"0.0147"},{"Metabolite":"Octenoylcarnitine","S00015097":"0.0000","S00015098":"0.0018","S00015099":"0.0026","S00015100":"0.0000","S00015094":"0.0020","S00015095":"0.0000","S00015096":"0.0000"},{"Metabolite":"Oleoylcarnitine","S00015097":"0.0017","S00015098":"0.0016","S00015099":"0.0014","S00015100":"0.0009","S00015094":"0.0029","S00015095":"0.0047","S00015096":"0.0018"},{"Metabolite":"Palmitoleylcarnitine","S00015097":"0.4843","S00015098":"0.6258","S00015099":"0.9740","S00015100":"0.4549","S00015094":"0.8579","S00015095":"0.9000","S00015096":"0.7468"},{"Metabolite":"palmitoylcarnitine","S00015097":"0.0463","S00015098":"0.0161","S00015099":"0.1122","S00015100":"0.0572","S00015094":"0.0395","S00015095":"0.0516","S00015096":"0.0200"},{"Metabolite":"propionylcarnitine","S00015097":"2.1411","S00015098":"3.7948","S00015099":"5.1150","S00015100":"2.3553","S00015094":"0.5882","S00015095":"0.5008","S00015096":"0.7013"},{"Metabolite":"Stearoylcarnitine","S00015097":"0.0040","S00015098":"0.0033","S00015099":"0.0082","S00015100":"0.0050","S00015094":"0.0078","S00015095":"0.0109","S00015096":"0.0096"},{"Metabolite":"Tetradecadiencarnitine","S00015097":"0.0000","S00015098":"0.0000","S00015099":"0.0024","S00015100":"0.0010","S00015094":"0.0000","S00015095":"0.0000","S00015096":"0.0000"},{"Metabolite":"Tetradecenoylcarnitine","S00015097":"0.1690","S00015098":"0.1923","S00015099":"1.0041","S00015100":"0.3620","S00015094":"0.0890","S00015095":"0.0610","S00015096":"0.0715"}],

"Metabolites":[{"Metabolite":"3-hydroxylauroylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"71464535","inchi_key":"","kegg_id":"","other_id":"C12-OH","other_id_type":"UM_Target_ID"},{"Metabolite":"3-Hydroxylinoleoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"78076335","inchi_key":"","kegg_id":"","other_id":"C18:2-OH","other_id_type":"UM_Target_ID"},{"Metabolite":"3-hydroxymyristoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"71464541","inchi_key":"","kegg_id":"","other_id":"C14-OH","other_id_type":"UM_Target_ID"},{"Metabolite":"3-Hydroxypalmitoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"71464553","inchi_key":"","kegg_id":"","other_id":"C16-OH","other_id_type":"UM_Target_ID"},{"Metabolite":"Acetylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"1","inchi_key":"","kegg_id":"","other_id":"C2","other_id_type":"UM_Target_ID"},{"Metabolite":"arachidonoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"71464486","inchi_key":"","kegg_id":"","other_id":"C20:4","other_id_type":"UM_Target_ID"},{"Metabolite":"butyrylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"439829","inchi_key":"","kegg_id":"","other_id":"C4","other_id_type":"UM_Target_ID"},{"Metabolite":"Decanoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"3332882","inchi_key":"","kegg_id":"","other_id":"C10:0","other_id_type":"UM_Target_ID"},{"Metabolite":"decenoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"54074509","inchi_key":"","kegg_id":"","other_id":"C10:1","other_id_type":"UM_Target_ID"},{"Metabolite":"Dodecenoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"53481671","inchi_key":"","kegg_id":"","other_id":"C12:1","other_id_type":"UM_Target_ID"},{"Metabolite":"Glutarylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"71317118","inchi_key":"","kegg_id":"","other_id":"C5:0-DC","other_id_type":"UM_Target_ID"},{"Metabolite":"Hexanoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6426853","inchi_key":"","kegg_id":"","other_id":"C6:0","other_id_type":"UM_Target_ID"},{"Metabolite":"icosadienoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"163425699","inchi_key":"","kegg_id":"","other_id":"C20:2","other_id_type":"UM_Target_ID"},{"Metabolite":"Icosanoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"53477833","inchi_key":"","kegg_id":"","other_id":"C20","other_id_type":"UM_Target_ID"},{"Metabolite":"icosatrienoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"C20:3","other_id_type":"UM_Target_ID"},{"Metabolite":"icoseneoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"71464507","inchi_key":"","kegg_id":"","other_id":"C20:1","other_id_type":"UM_Target_ID"},{"Metabolite":"isovalerylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6426852","inchi_key":"","kegg_id":"","other_id":"C5:0","other_id_type":"UM_Target_ID"},{"Metabolite":"lauroylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"168381","inchi_key":"","kegg_id":"","other_id":"C12:0","other_id_type":"UM_Target_ID"},{"Metabolite":"L-Carnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"10917","inchi_key":"","kegg_id":"","other_id":"L-Carnitine","other_id_type":"UM_Target_ID"},{"Metabolite":"Linoelaidyl carnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"53477834","inchi_key":"","kegg_id":"","other_id":"C18:2","other_id_type":"UM_Target_ID"},{"Metabolite":"myristoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"3413344","inchi_key":"","kegg_id":"","other_id":"C14:0","other_id_type":"UM_Target_ID"},{"Metabolite":"octanoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"123701","inchi_key":"","kegg_id":"","other_id":"C8:0","other_id_type":"UM_Target_ID"},{"Metabolite":"Octenoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"71464472","inchi_key":"","kegg_id":"","other_id":"C8:1","other_id_type":"UM_Target_ID"},{"Metabolite":"Oleoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"166952","inchi_key":"","kegg_id":"","other_id":"C18:1","other_id_type":"UM_Target_ID"},{"Metabolite":"Palmitoleylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"76040365","inchi_key":"","kegg_id":"","other_id":"C16:1","other_id_type":"UM_Target_ID"},{"Metabolite":"palmitoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"461","inchi_key":"","kegg_id":"","other_id":"C16:0","other_id_type":"UM_Target_ID"},{"Metabolite":"propionylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"107738","inchi_key":"","kegg_id":"","other_id":"C3","other_id_type":"UM_Target_ID"},{"Metabolite":"Stearoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5282","inchi_key":"","kegg_id":"","other_id":"C18:0","other_id_type":"UM_Target_ID"},{"Metabolite":"Tetradecadiencarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"53481681","inchi_key":"","kegg_id":"","other_id":"C14:2","other_id_type":"UM_Target_ID"},{"Metabolite":"Tetradecenoylcarnitine","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"22833575","inchi_key":"","kegg_id":"","other_id":"C14:1","other_id_type":"UM_Target_ID"}]
}

}