{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000215","ANALYSIS_ID":"AN000317","VERSION":"1","CREATED_ON":"01-15-2019"},

"PROJECT":{"PROJECT_TITLE":"Regulation of hepatic metabolism","PROJECT_SUMMARY":"Examine metabolite in liver and plasma before and after 13C-glucose load in wild type, LIRKO and LIRFKO mice","INSTITUTE":"University of Chicago","DEPARTMENT":"Jesse Brown VA Medical Center","LABORATORY":"Unterman Lab","LAST_NAME":"Unterman","FIRST_NAME":"Terry","ADDRESS":"Chicago, IL","EMAIL":"unterman@uic.edu","PHONE":"847-778-0943","DOI":"http://dx.doi.org/10.21228/M8N88K"},

"STUDY":{"STUDY_TITLE":"Liver and Plasma metaboites for 13C-glucose load in wild type, LIRKO GTT 1 mice","STUDY_TYPE":"13C mass isotopomer analysis (flux studies)","STUDY_SUMMARY":"Mice were fasted for 18 hr overnight then sacrificed or treated with 13C-U-glucose (2 g/kg ip) and sacrificed 1 hr later by decapitation and liver was immediately freeze-clamped and stored in liquid N2 and then at -80 C.  Wild type (IR and IR/FoxO1 floxed) mice were sacrificed after fasting and 1 hr post-glucose treatment.  Liver-specific insulin receptor knockout (LIRKO) and insulin receptor/FoxO1 double knockout (LIRFKO) mice were sacrificed 1 hr post glucose treatment. http://www.nature.com/ncomms/2015/150512/ncomms8079/full/ncomms8079.html","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@umich.edu","PHONE":"-"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0003002",
"Sample ID":"S00013145",
"Factors":{"treatment":"glucose","Strain":"IRF floxed"}
},
{
"Subject ID":"SU0003001",
"Sample ID":"S00013143",
"Factors":{"treatment":"glucose","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003001",
"Sample ID":"S00013142",
"Factors":{"treatment":"glucose","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003001",
"Sample ID":"S00013144",
"Factors":{"treatment":"glucose","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003004",
"Sample ID":"S00013152",
"Factors":{"treatment":"glucose","Strain":"LIRFKO"}
},
{
"Subject ID":"SU0003004",
"Sample ID":"S00013153",
"Factors":{"treatment":"glucose","Strain":"LIRFKO"}
},
{
"Subject ID":"SU0003004",
"Sample ID":"S00013154",
"Factors":{"treatment":"glucose","Strain":"LIRFKO"}
},
{
"Subject ID":"SU0003004",
"Sample ID":"S00013155",
"Factors":{"treatment":"glucose","Strain":"LIRFKO"}
},
{
"Subject ID":"SU0003003",
"Sample ID":"S00013146",
"Factors":{"treatment":"glucose","Strain":"LIRKO"}
},
{
"Subject ID":"SU0003003",
"Sample ID":"S00013147",
"Factors":{"treatment":"glucose","Strain":"LIRKO"}
},
{
"Subject ID":"SU0003003",
"Sample ID":"S00013148",
"Factors":{"treatment":"glucose","Strain":"LIRKO"}
},
{
"Subject ID":"SU0003003",
"Sample ID":"S00013149",
"Factors":{"treatment":"glucose","Strain":"LIRKO"}
},
{
"Subject ID":"SU0003003",
"Sample ID":"S00013150",
"Factors":{"treatment":"glucose","Strain":"LIRKO"}
},
{
"Subject ID":"SU0003003",
"Sample ID":"S00013151",
"Factors":{"treatment":"glucose","Strain":"LIRKO"}
},
{
"Subject ID":"SU0003000",
"Sample ID":"S00013136",
"Factors":{"treatment":"none","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003000",
"Sample ID":"S00013137",
"Factors":{"treatment":"none","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003000",
"Sample ID":"S00013138",
"Factors":{"treatment":"none","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003000",
"Sample ID":"S00013139",
"Factors":{"treatment":"none","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003000",
"Sample ID":"S00013140",
"Factors":{"treatment":"none","Strain":"IR floxed"}
},
{
"Subject ID":"SU0003000",
"Sample ID":"S00013141",
"Factors":{"treatment":"none","Strain":"IR floxed"}
}
],
"COLLECTION":{"SAMPLE_TYPE":"Liver","COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_PROTOCOL_FILENAME":"Fluxomics_analysis_protocol-2015-03-11.docx","SAMPLEPREP_SUMMARY":"-"},

"CHROMATOGRAPHY":{"METHODS_ID":"AQM010","METHODS_FILENAME":"QTOF-002-HILIC-35min-1mm.m.zip","INSTRUMENT_NAME":"Agilent 1260","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)","CHROMATOGRAPHY_TYPE":"HILIC"},

"ANALYSIS":{"LABORATORY_NAME":"MRC2 (University of Michigan)","ANALYSIS_TYPE":"MS","ACQUISITION_PARAMETERS_FILE":"QTOF-002-HILIC-35min-1mm.m.zip","PROCESSING_PARAMETERS_FILE":"EX00258-flux-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6520 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"Area",

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