{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000287","ANALYSIS_ID":"AN000456","VERSION":"1","CREATED_ON":"December 26, 2015, 12:12 pm"},

"PROJECT":{"PROJECT_TITLE":"Energetics in dystrophic muscle","PROJECT_TYPE":"Cross-sectional","PROJECT_SUMMARY":"This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.","INSTITUTE":"University of Florida","DEPARTMENT":"Physical Therapy","LABORATORY":"Muscle Physiology Laboratory","LAST_NAME":"Forbes","FIRST_NAME":"Sean","ADDRESS":"University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC","EMAIL":"scforbes@ufl.edu","PHONE":"352-273-6111","FUNDING_SOURCE":"Start-up funds"},

"STUDY":{"STUDY_TITLE":"Energetics in dystrophic muscle","STUDY_TYPE":"Cross-sectional","STUDY_SUMMARY":"This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LABORATORY":"Muscle Physiology Laboratory","LAST_NAME":"Forbes","FIRST_NAME":"Sean","ADDRESS":"University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC","EMAIL":"scforbes@ufl.edu","PHONE":"352-273-6111","NUM_GROUPS":"4"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Jackson Lab","ANIMAL_HOUSING":"McKnight Brain Institute, UF","ANIMAL_LIGHT_CYCLE":"12 hr light/12 hr dark","ANIMAL_FEED":"ad libtum","ANIMAL_WATER":"ad libtum","ANIMAL_INCLUSION_CRITERIA":"males only"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"scf_01-M1",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M2",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M3",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M4",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M5",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M1",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M2",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M3",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M4",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M5",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M1",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M2",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M3",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M4",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M5",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M1",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M2",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M3",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M4",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M5",
"Factors":{"Strain":"C57Bl/10"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C","COLLECTION_PROTOCOL_COMMENTS":"L3-190","COLLECTION_METHOD":"muscle extraction, frozen","COLLECTION_LOCATION":"UF, musle physiology laboratory","COLLECTION_FREQUENCY":"once/mouse","COLLECTION_DURATION":"Total ~5 min","COLLECTION_TIME":"no dose","VOLUMEORAMOUNT_COLLECTED":"entire muscles extracted","STORAGE_CONDITIONS":"-80C freezer","COLLECTION_VIALS":"2 ml vials","STORAGE_VIALS":"2 ml vials","COLLECTION_TUBE_TEMP":"-80C","ADDITIVES":"none","BLOOD_SERUM_OR_PLASMA":"none","TISSUE_CELL_IDENTIFICATION":"none","TISSUE_CELL_QUANTITY_TAKEN":"none"},

"TREATMENT":{"TREATMENT_SUMMARY":"No treatment"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"NAD/ATP or NADH","SAMPLEPREP_PROTOCOL_FILENAME":"SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"NAD-ATP method","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290 Infinity","COLUMN_NAME":"Thermo Hypercarb (50 x 2.1mm, 3um)","METHODS_FILENAME":"SB_NAD-ATP_Assay_Agilent.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"NAD/ATP","INSTRUMENT_NAME":"Agilent 6490 QQQ Agilent 6490"},

"MS_METABOLITE_DATA":{
"Units":"pmol/mg dry powder",

"Data":[{"Metabolite":"NMN","scf_01-M1":"","scf_01-M2":"2.57","scf_01-M3":"","scf_01-M4":"3.26","scf_01-M5":"3.32","scf_02-M1":"3.57","scf_02-M2":"2.49","scf_02-M3":"3.30","scf_02-M4":"","scf_02-M5":"5.35","scf_03-M1":"3.63","scf_03-M2":"","scf_03-M3":"3.16","scf_03-M4":"3.40","scf_03-M5":"5.17","scf_04-M1":"2.50","scf_04-M2":"2.91","scf_04-M3":"2.67","scf_04-M4":"2.27","scf_04-M5":"2.97"},{"Metabolite":"NAD","scf_01-M1":"1208.52","scf_01-M2":"1601.82","scf_01-M3":"1484.41","scf_01-M4":"1837.65","scf_01-M5":"1892.69","scf_02-M1":"1772.16","scf_02-M2":"1732.87","scf_02-M3":"1157.78","scf_02-M4":"1392.64","scf_02-M5":"1710.87","scf_03-M1":"1209.64","scf_03-M2":"852.58","scf_03-M3":"1478.12","scf_03-M4":"1416.72","scf_03-M5":"924.83","scf_04-M1":"2118.29","scf_04-M2":"2313.90","scf_04-M3":"2318.14","scf_04-M4":"2186.24","scf_04-M5":"2341.62"},{"Metabolite":"NADP","scf_01-M1":"","scf_01-M2":"","scf_01-M3":"","scf_01-M4":"","scf_01-M5":"37.08","scf_02-M1":"","scf_02-M2":"","scf_02-M3":"","scf_02-M4":"","scf_02-M5":"42.88","scf_03-M1":"","scf_03-M2":"","scf_03-M3":"34.52","scf_03-M4":"","scf_03-M5":"","scf_04-M1":"","scf_04-M2":"","scf_04-M3":"","scf_04-M4":"","scf_04-M5":""},{"Metabolite":"AMP","scf_01-M1":"0.61","scf_01-M2":"0.34","scf_01-M3":"0.62","scf_01-M4":"0.35","scf_01-M5":"0.47","scf_02-M1":"0.51","scf_02-M2":"0.34","scf_02-M3":"0.36","scf_02-M4":"0.45","scf_02-M5":"0.27","scf_03-M1":"0.33","scf_03-M2":"1.18","scf_03-M3":"0.22","scf_03-M4":"0.23","scf_03-M5":"0.34","scf_04-M1":"","scf_04-M2":"0.19","scf_04-M3":"","scf_04-M4":"","scf_04-M5":"0.32"},{"Metabolite":"ADP","scf_01-M1":"4.16","scf_01-M2":"3.50","scf_01-M3":"4.70","scf_01-M4":"3.57","scf_01-M5":"4.01","scf_02-M1":"4.36","scf_02-M2":"4.07","scf_02-M3":"3.08","scf_02-M4":"4.16","scf_02-M5":"3.66","scf_03-M1":"3.33","scf_03-M2":"4.20","scf_03-M3":"3.02","scf_03-M4":"3.13","scf_03-M5":"2.66","scf_04-M1":"2.72","scf_04-M2":"3.30","scf_04-M3":"3.11","scf_04-M4":"3.23","scf_04-M5":"3.64"},{"Metabolite":"ATP","scf_01-M1":"17.74","scf_01-M2":"25.06","scf_01-M3":"22.81","scf_01-M4":"24.31","scf_01-M5":"20.41","scf_02-M1":"24.48","scf_02-M2":"26.02","scf_02-M3":"14.27","scf_02-M4":"18.93","scf_02-M5":"23.10","scf_03-M1":"16.90","scf_03-M2":"10.52","scf_03-M3":"18.07","scf_03-M4":"16.88","scf_03-M5":"10.84","scf_04-M1":"23.07","scf_04-M2":"26.83","scf_04-M3":"24.82","scf_04-M4":"27.29","scf_04-M5":"26.14"}],

"Metabolites":[{"Metabolite":"NMN","Retention Index":"1.20","Moverz Quant":"335.2, 123.1","PubChem CID":"14180"},{"Metabolite":"NAD","Retention Index":"1.80","Moverz Quant":"664.2, 136.1","PubChem CID":"5893"},{"Metabolite":"NADP","Retention Index":"1.70","Moverz Quant":"744.2, 136.1","PubChem CID":"5886"},{"Metabolite":"AMP","Retention Index":"1.50","Moverz Quant":"348.1, 136.1","PubChem CID":"6083"},{"Metabolite":"ADP","Retention Index":"1.50","Moverz Quant":"428.0, 136.1","PubChem CID":"6022"},{"Metabolite":"ATP","Retention Index":"1.50","Moverz Quant":"508.1, 136.1","PubChem CID":"5957"}]
}

}