{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000287","ANALYSIS_ID":"AN000457","VERSION":"1","CREATED_ON":"December 26, 2015, 12:12 pm"},

"PROJECT":{"PROJECT_TITLE":"Energetics in dystrophic muscle","PROJECT_TYPE":"Cross-sectional","PROJECT_SUMMARY":"This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.","INSTITUTE":"University of Florida","DEPARTMENT":"Physical Therapy","LABORATORY":"Muscle Physiology Laboratory","LAST_NAME":"Forbes","FIRST_NAME":"Sean","ADDRESS":"University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC","EMAIL":"scforbes@ufl.edu","PHONE":"352-273-6111","FUNDING_SOURCE":"Start-up funds"},

"STUDY":{"STUDY_TITLE":"Energetics in dystrophic muscle","STUDY_TYPE":"Cross-sectional","STUDY_SUMMARY":"This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LABORATORY":"Muscle Physiology Laboratory","LAST_NAME":"Forbes","FIRST_NAME":"Sean","ADDRESS":"University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC","EMAIL":"scforbes@ufl.edu","PHONE":"352-273-6111","NUM_GROUPS":"4"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Jackson Lab","ANIMAL_HOUSING":"McKnight Brain Institute, UF","ANIMAL_LIGHT_CYCLE":"12 hr light/12 hr dark","ANIMAL_FEED":"ad libtum","ANIMAL_WATER":"ad libtum","ANIMAL_INCLUSION_CRITERIA":"males only"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"scf_01-M1",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M2",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M3",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M4",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_01-M5",
"Factors":{"Strain":"nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M1",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M2",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M3",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M4",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_02-M5",
"Factors":{"Strain":"mdx-nNOS-/-"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M1",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M2",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M3",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M4",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_03-M5",
"Factors":{"Strain":"mdx"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M1",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M2",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M3",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M4",
"Factors":{"Strain":"C57Bl/10"}
},
{
"Subject ID":"-",
"Sample ID":"scf_04-M5",
"Factors":{"Strain":"C57Bl/10"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C","COLLECTION_PROTOCOL_COMMENTS":"L3-190","COLLECTION_METHOD":"muscle extraction, frozen","COLLECTION_LOCATION":"UF, musle physiology laboratory","COLLECTION_FREQUENCY":"once/mouse","COLLECTION_DURATION":"Total ~5 min","COLLECTION_TIME":"no dose","VOLUMEORAMOUNT_COLLECTED":"entire muscles extracted","STORAGE_CONDITIONS":"-80C freezer","COLLECTION_VIALS":"2 ml vials","STORAGE_VIALS":"2 ml vials","COLLECTION_TUBE_TEMP":"-80C","ADDITIVES":"none","BLOOD_SERUM_OR_PLASMA":"none","TISSUE_CELL_IDENTIFICATION":"none","TISSUE_CELL_QUANTITY_TAKEN":"none"},

"TREATMENT":{"TREATMENT_SUMMARY":"No treatment"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"NAD/ATP or NADH","SAMPLEPREP_PROTOCOL_FILENAME":"SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"NAD-ATP method","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290 Infinity","COLUMN_NAME":"Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)","METHODS_FILENAME":"SB_NADH_Assay_Agilent.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"NADH","INSTRUMENT_NAME":"Agilent 6490 QQQ Agilent 6490"},

"MS_METABOLITE_DATA":{
"Units":"pmol/mg dry powder",

"Data":[{"Metabolite":"NADH","scf_01-M1":"90.08","scf_01-M2":"128.62","scf_01-M3":"94.34","scf_01-M4":"65.15","scf_01-M5":"155.71","scf_02-M1":"99.68","scf_02-M2":"125.30","scf_02-M3":"34.37","scf_02-M4":"55.37","scf_02-M5":"76.05","scf_03-M1":"56.18","scf_03-M2":"","scf_03-M3":"64.50","scf_03-M4":"115.93","scf_03-M5":"17.44","scf_04-M1":"184.07","scf_04-M2":"189.04","scf_04-M3":"198.01","scf_04-M4":"139.52","scf_04-M5":"150.27"}],

"Metabolites":[{"Metabolite":"NADH","Retention Index":"1.80","Moverz Quant":"666.2, 649.2","PubChem CID":"439153"}]
}

}