{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000287","ANALYSIS_ID":"AN000457","VERSION":"1","CREATED_ON":"December 26, 2015, 12:12 pm"}, "PROJECT":{"PROJECT_TITLE":"Energetics in dystrophic muscle","PROJECT_TYPE":"Cross-sectional","PROJECT_SUMMARY":"This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.","INSTITUTE":"University of Florida","DEPARTMENT":"Physical Therapy","LABORATORY":"Muscle Physiology Laboratory","LAST_NAME":"Forbes","FIRST_NAME":"Sean","ADDRESS":"University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC","EMAIL":"scforbes@ufl.edu","PHONE":"352-273-6111","FUNDING_SOURCE":"Start-up funds"}, "STUDY":{"STUDY_TITLE":"Energetics in dystrophic muscle","STUDY_TYPE":"Cross-sectional","STUDY_SUMMARY":"This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LABORATORY":"Muscle Physiology Laboratory","LAST_NAME":"Forbes","FIRST_NAME":"Sean","ADDRESS":"University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC","EMAIL":"scforbes@ufl.edu","PHONE":"352-273-6111","NUM_GROUPS":"4"}, "SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Jackson Lab","ANIMAL_HOUSING":"McKnight Brain Institute, UF","ANIMAL_LIGHT_CYCLE":"12 hr light/12 hr dark","ANIMAL_FEED":"ad libtum","ANIMAL_WATER":"ad libtum","ANIMAL_INCLUSION_CRITERIA":"males only"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"scf_01-M1", "Factors":{"Strain":"nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_01-M2", "Factors":{"Strain":"nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_01-M3", "Factors":{"Strain":"nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_01-M4", "Factors":{"Strain":"nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_01-M5", "Factors":{"Strain":"nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_02-M1", "Factors":{"Strain":"mdx-nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_02-M2", "Factors":{"Strain":"mdx-nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_02-M3", "Factors":{"Strain":"mdx-nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_02-M4", "Factors":{"Strain":"mdx-nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_02-M5", "Factors":{"Strain":"mdx-nNOS-/-"} }, { "Subject ID":"-", "Sample ID":"scf_03-M1", "Factors":{"Strain":"mdx"} }, { "Subject ID":"-", "Sample ID":"scf_03-M2", "Factors":{"Strain":"mdx"} }, { "Subject ID":"-", "Sample ID":"scf_03-M3", "Factors":{"Strain":"mdx"} }, { "Subject ID":"-", "Sample ID":"scf_03-M4", "Factors":{"Strain":"mdx"} }, { "Subject ID":"-", "Sample ID":"scf_03-M5", "Factors":{"Strain":"mdx"} }, { "Subject ID":"-", "Sample ID":"scf_04-M1", "Factors":{"Strain":"C57Bl/10"} }, { "Subject ID":"-", "Sample ID":"scf_04-M2", "Factors":{"Strain":"C57Bl/10"} }, { "Subject ID":"-", "Sample ID":"scf_04-M3", "Factors":{"Strain":"C57Bl/10"} }, { "Subject ID":"-", "Sample ID":"scf_04-M4", "Factors":{"Strain":"C57Bl/10"} }, { "Subject ID":"-", "Sample ID":"scf_04-M5", "Factors":{"Strain":"C57Bl/10"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C","COLLECTION_PROTOCOL_COMMENTS":"L3-190","COLLECTION_METHOD":"muscle extraction, frozen","COLLECTION_LOCATION":"UF, musle physiology laboratory","COLLECTION_FREQUENCY":"once/mouse","COLLECTION_DURATION":"Total ~5 min","COLLECTION_TIME":"no dose","VOLUMEORAMOUNT_COLLECTED":"entire muscles extracted","STORAGE_CONDITIONS":"-80C freezer","COLLECTION_VIALS":"2 ml vials","STORAGE_VIALS":"2 ml vials","COLLECTION_TUBE_TEMP":"-80C","ADDITIVES":"none","BLOOD_SERUM_OR_PLASMA":"none","TISSUE_CELL_IDENTIFICATION":"none","TISSUE_CELL_QUANTITY_TAKEN":"none"}, "TREATMENT":{"TREATMENT_SUMMARY":"No treatment"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"NAD/ATP or NADH","SAMPLEPREP_PROTOCOL_FILENAME":"SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"NAD-ATP method","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290 Infinity","COLUMN_NAME":"Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)","METHODS_FILENAME":"SB_NADH_Assay_Agilent.pdf"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"NADH","INSTRUMENT_NAME":"Agilent 6490 QQQ Agilent 6490"}, "MS_METABOLITE_DATA":{ "Units":"pmol/mg dry powder", "Data":[{"Metabolite":"NADH","scf_01-M1":"90.08","scf_01-M2":"128.62","scf_01-M3":"94.34","scf_01-M4":"65.15","scf_01-M5":"155.71","scf_02-M1":"99.68","scf_02-M2":"125.30","scf_02-M3":"34.37","scf_02-M4":"55.37","scf_02-M5":"76.05","scf_03-M1":"56.18","scf_03-M2":"","scf_03-M3":"64.50","scf_03-M4":"115.93","scf_03-M5":"17.44","scf_04-M1":"184.07","scf_04-M2":"189.04","scf_04-M3":"198.01","scf_04-M4":"139.52","scf_04-M5":"150.27"}], "Metabolites":[{"Metabolite":"NADH","Retention Index":"1.80","Moverz Quant":"666.2, 649.2","PubChem CID":"439153"}] } }