{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000295","ANALYSIS_ID":"AN000472","VERSION":"1","CREATED_ON":"December 28, 2015, 6:45 am"},

"PROJECT":{"PROJECT_TITLE":"Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis","PROJECT_SUMMARY":"Analysis of wild type lung tissue after saline or bleomycin treatment","INSTITUTE":"University of Michigan","LAST_NAME":"Hu","FIRST_NAME":"Biao","ADDRESS":"Ann Arbor, MI","EMAIL":"biaohu@med.umich.edu","PHONE":"734-763-5731"},

"STUDY":{"STUDY_TITLE":"Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human Normal Lung Fiboblasts with TGFbeta and Fizz2 treatment","STUDY_SUMMARY":"Glycolysis/TCA/Nucleotide analysis and NAD+ and related metabolite analysis for all samples","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"6","TOTAL_SUBJECTS":"35"},

"SUBJECT":{"SUBJECT_TYPE":"Animal/Human","SUBJECT_SPECIES":"Mus musculus;Homo sapiens"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0005146",
"Sample ID":"S00016590",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"saline"}
},
{
"Subject ID":"SU0005147",
"Sample ID":"S00016591",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"saline"}
},
{
"Subject ID":"SU0005148",
"Sample ID":"S00016592",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"saline"}
},
{
"Subject ID":"SU0005149",
"Sample ID":"S00016593",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"saline"}
},
{
"Subject ID":"SU0005150",
"Sample ID":"S00016594",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"saline"}
},
{
"Subject ID":"SU0005151",
"Sample ID":"S00016595",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005152",
"Sample ID":"S00016596",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005153",
"Sample ID":"S00016597",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005154",
"Sample ID":"S00016598",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005155",
"Sample ID":"S00016599",
"Factors":{"Species":"Mus musculus","Genotype":"WT","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005156",
"Sample ID":"S00016600",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"saline"}
},
{
"Subject ID":"SU0005157",
"Sample ID":"S00016601",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"saline"}
},
{
"Subject ID":"SU0005158",
"Sample ID":"S00016602",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"saline"}
},
{
"Subject ID":"SU0005159",
"Sample ID":"S00016603",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"saline"}
},
{
"Subject ID":"SU0005160",
"Sample ID":"S00016604",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"saline"}
},
{
"Subject ID":"SU0005161",
"Sample ID":"S00016605",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005162",
"Sample ID":"S00016606",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005163",
"Sample ID":"S00016607",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005164",
"Sample ID":"S00016608",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005165",
"Sample ID":"S00016609",
"Factors":{"Species":"Mus musculus","Genotype":"PARP1 knockout","Treatment":"bleomycin"}
},
{
"Subject ID":"SU0005166",
"Sample ID":"S00016610",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"None"}
},
{
"Subject ID":"SU0005167",
"Sample ID":"S00016611",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"None"}
},
{
"Subject ID":"SU0005168",
"Sample ID":"S00016612",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"None"}
},
{
"Subject ID":"SU0005169",
"Sample ID":"S00016613",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"None"}
},
{
"Subject ID":"SU0005170",
"Sample ID":"S00016614",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"None"}
},
{
"Subject ID":"SU0005171",
"Sample ID":"S00016615",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"TGFb"}
},
{
"Subject ID":"SU0005172",
"Sample ID":"S00016616",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"TGFb"}
},
{
"Subject ID":"SU0005173",
"Sample ID":"S00016617",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"TGFb"}
},
{
"Subject ID":"SU0005174",
"Sample ID":"S00016618",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"TGFb"}
},
{
"Subject ID":"SU0005175",
"Sample ID":"S00016619",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"TGFb"}
},
{
"Subject ID":"SU0005176",
"Sample ID":"S00016620",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"Fizz2"}
},
{
"Subject ID":"SU0005177",
"Sample ID":"S00016621",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"Fizz2"}
},
{
"Subject ID":"SU0005178",
"Sample ID":"S00016622",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"Fizz2"}
},
{
"Subject ID":"SU0005179",
"Sample ID":"S00016623",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"Fizz2"}
},
{
"Subject ID":"SU0005180",
"Sample ID":"S00016624",
"Factors":{"Species":"Homo sapiens","Genotype":"WT","Treatment":"Fizz2"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"Glycolysis-TCA-nucleotides-20150324.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1260","COLUMN_NAME":"Waters XBridge C18 (50 x 2.1mm, 3um)","METHODS_FILENAME":"Ceramides-20150305.docx","METHODS_ID":"DQM0152"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Ceramides MS(+)","INSTRUMENT_NAME":"Agilent 6410A QQQ Agilent 6410A QQQ"},

"MS_METABOLITE_DATA":{
"Units":"ng/ug protein",

"Data":[{"Metabolite":"Cer C14","S00016590":"93.288","S00016591":"101.353","S00016592":"103.542","S00016593":"125.486","S00016594":"140.060","S00016595":"154.471","S00016596":"201.662","S00016597":"155.359","S00016598":"73.501","S00016599":"162.947","S00016600":"135.161","S00016601":"129.968","S00016602":"98.227","S00016603":"132.268","S00016604":"152.251","S00016605":"161.141","S00016606":"111.575","S00016607":"106.159","S00016608":"152.067","S00016609":"101.588","S00016610":"155.382","S00016611":"261.432","S00016612":"378.889","S00016613":"385.095","S00016614":"237.200","S00016615":"300.852","S00016616":"322.175","S00016617":"297.417","S00016618":"251.528","S00016619":"200.446","S00016620":"215.256","S00016621":"277.997","S00016622":"274.900","S00016623":"492.692","S00016624":"411.681"},{"Metabolite":"Cer C16","S00016590":"156.292","S00016591":"127.118","S00016592":"179.486","S00016593":"190.264","S00016594":"208.758","S00016595":"237.578","S00016596":"251.387","S00016597":"223.935","S00016598":"129.219","S00016599":"195.350","S00016600":"259.262","S00016601":"194.173","S00016602":"155.850","S00016603":"199.311","S00016604":"237.022","S00016605":"199.104","S00016606":"137.600","S00016607":"131.858","S00016608":"198.442","S00016609":"162.748","S00016610":"155.953","S00016611":"307.779","S00016612":"383.478","S00016613":"454.916","S00016614":"318.123","S00016615":"357.642","S00016616":"311.316","S00016617":"368.992","S00016618":"304.137","S00016619":"264.793","S00016620":"290.305","S00016621":"356.363","S00016622":"247.555","S00016623":"497.786","S00016624":"494.510"},{"Metabolite":"Cer C18","S00016590":"63.789","S00016591":"63.740","S00016592":"76.727","S00016593":"86.257","S00016594":"89.406","S00016595":"91.469","S00016596":"82.874","S00016597":"89.978","S00016598":"56.767","S00016599":"67.920","S00016600":"98.494","S00016601":"60.876","S00016602":"61.456","S00016603":"72.299","S00016604":"96.954","S00016605":"69.014","S00016606":"57.414","S00016607":"54.886","S00016608":"86.672","S00016609":"78.614","S00016610":"99.899","S00016611":"172.728","S00016612":"222.884","S00016613":"264.461","S00016614":"185.460","S00016615":"217.412","S00016616":"201.513","S00016617":"213.863","S00016618":"166.862","S00016619":"145.998","S00016620":"171.378","S00016621":"213.614","S00016622":"160.240","S00016623":"326.282","S00016624":"295.957"},{"Metabolite":"Cer C18:1","S00016590":"2.000","S00016591":"1.935","S00016592":"2.312","S00016593":"2.793","S00016594":"2.551","S00016595":"3.645","S00016596":"2.068","S00016597":"2.533","S00016598":"1.512","S00016599":"1.296","S00016600":"3.024","S00016601":"1.615","S00016602":"1.654","S00016603":"1.787","S00016604":"2.328","S00016605":"1.984","S00016606":"1.217","S00016607":"1.052","S00016608":"1.989","S00016609":"2.398","S00016610":"2.840","S00016611":"5.707","S00016612":"8.151","S00016613":"8.302","S00016614":"6.284","S00016615":"6.760","S00016616":"6.662","S00016617":"8.066","S00016618":"5.510","S00016619":"5.259","S00016620":"6.104","S00016621":"7.789","S00016622":"5.223","S00016623":"10.173","S00016624":"9.416"},{"Metabolite":"Cer C20","S00016590":"70.328","S00016591":"69.333","S00016592":"90.764","S00016593":"104.009","S00016594":"108.344","S00016595":"108.613","S00016596":"96.793","S00016597":"92.643","S00016598":"69.257","S00016599":"88.966","S00016600":"110.143","S00016601":"69.808","S00016602":"70.775","S00016603":"74.115","S00016604":"89.809","S00016605":"90.507","S00016606":"79.281","S00016607":"86.101","S00016608":"122.261","S00016609":"99.257","S00016610":"110.621","S00016611":"228.758","S00016612":"234.373","S00016613":"328.060","S00016614":"242.337","S00016615":"267.997","S00016616":"250.882","S00016617":"308.470","S00016618":"243.249","S00016619":"186.254","S00016620":"225.505","S00016621":"288.603","S00016622":"217.809","S00016623":"410.320","S00016624":"385.374"},{"Metabolite":"Cer C22","S00016590":"249246.300","S00016591":"197007.476","S00016592":"515291.883","S00016593":"355460.976","S00016594":"464656.841","S00016595":"365254.181","S00016596":"510307.001","S00016597":"312255.895","S00016598":"315255.764","S00016599":"488964.969","S00016600":"736807.099","S00016601":"493406.892","S00016602":"282547.056","S00016603":"387942.959","S00016604":"458349.476","S00016605":"496853.384","S00016606":"219198.867","S00016607":"343588.209","S00016608":"658433.474","S00016609":"491547.356","S00016610":"234580.372","S00016611":"428701.027","S00016612":"728329.987","S00016613":"721773.987","S00016614":"614381.348","S00016615":"492752.563","S00016616":"666927.938","S00016617":"859299.330","S00016618":"607305.494","S00016619":"450409.594","S00016620":"616280.493","S00016621":"684041.466","S00016622":"570624.478","S00016623":"881893.200","S00016624":"633436.779"},{"Metabolite":"Cer C24","S00016590":"148.883","S00016591":"125.673","S00016592":"200.816","S00016593":"148.731","S00016594":"217.472","S00016595":"210.894","S00016596":"309.305","S00016597":"167.150","S00016598":"124.048","S00016599":"295.245","S00016600":"234.005","S00016601":"192.946","S00016602":"115.978","S00016603":"180.340","S00016604":"212.509","S00016605":"219.166","S00016606":"244.737","S00016607":"138.430","S00016608":"279.632","S00016609":"203.872","S00016610":"136.343","S00016611":"410.319","S00016612":"578.833","S00016613":"644.635","S00016614":"371.498","S00016615":"445.406","S00016616":"465.718","S00016617":"538.066","S00016618":"416.276","S00016619":"179.728","S00016620":"181.825","S00016621":"225.026","S00016622":"214.018","S00016623":"674.257","S00016624":"397.511"},{"Metabolite":"Cer C24:1","S00016590":"943191.087","S00016591":"587489.776","S00016592":"1451552.522","S00016593":"1270213.796","S00016594":"1366797.675","S00016595":"1353068.120","S00016596":"1727224.089","S00016597":"1147270.861","S00016598":"1020504.433","S00016599":"1376437.803","S00016600":"2033184.497","S00016601":"1328641.249","S00016602":"987074.190","S00016603":"1132915.244","S00016604":"1399355.663","S00016605":"1393113.445","S00016606":"714921.394","S00016607":"1010625.816","S00016608":"1770983.500","S00016609":"1440687.416","S00016610":"550532.395","S00016611":"988602.202","S00016612":"1494584.401","S00016613":"1470959.388","S00016614":"1470856.218","S00016615":"1283520.304","S00016616":"1542765.927","S00016617":"1963191.691","S00016618":"1381850.103","S00016619":"1331870.778","S00016620":"1426053.842","S00016621":"1578535.966","S00016622":"1262880.766","S00016623":"1656101.332","S00016624":"1423790.665"}],

"Metabolites":[{"Metabolite":"Cer C14","PubChem CID":"5282310"},{"Metabolite":"Cer C16","PubChem CID":"5283564"},{"Metabolite":"Cer C18","PubChem CID":"5283565"},{"Metabolite":"Cer C18:1","PubChem CID":"5283563"},{"Metabolite":"Cer C20","PubChem CID":"5283566"},{"Metabolite":"Cer C22","PubChem CID":"5283567"},{"Metabolite":"Cer C24","PubChem CID":"5283571"},{"Metabolite":"Cer C24:1","PubChem CID":"5283568"}]
}

}