{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000304","ANALYSIS_ID":"AN000483","VERSION":"1","CREATED_ON":"December 29, 2015, 9:46 am"},

"PROJECT":{"PROJECT_TITLE":"Early in life exposure studies","PROJECT_SUMMARY":"Early in life exposures affect innate immune cells activation","INSTITUTE":"University of Michigan","DEPARTMENT":"Pediatrics","LABORATORY":"Hershenson Lab","LAST_NAME":"Popova","FIRST_NAME":"Antonia","ADDRESS":"Ann Arbor, MI","EMAIL":"antoniap@med.umich.edu","PHONE":"734-764-6736"},

"STUDY":{"STUDY_TITLE":"Early in life exposure studies on human and mouse samples","STUDY_SUMMARY":"Experiment1: 2 day old baby mice were exposed to hyperoxia (75% O2) continuously for 7 days. Control baby mice were housed in room air (normoxia). Plasma and bronchoalveolar lavage fluid (BALF) were harvested after 7 days of exposure (on Day of life 9). Experiment2: 2 day old baby mice were exposed to room air or hyperoxia for 14 days and subsequently treated with RV1 or sham.Plasma was collected 5 days after treatment. Human tracheal aspirates were collected from prematurely born infants undergoing mechanical ventilation for respiratory distress syndrome in the first week of life.Tracheal aspirate supernatants are submitted for the assay. We would like to measure adenosine, AMP, ADP and ATP levels.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"7","TOTAL_SUBJECTS":"52"},

"SUBJECT":{"SUBJECT_TYPE":"Human/animal","SUBJECT_SPECIES":"Mus musculus;Homo sapiens"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0011995",
"Sample ID":"S00017890",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0011996",
"Sample ID":"S00017891",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0011997",
"Sample ID":"S00017892",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0011998",
"Sample ID":"S00017893",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0011999",
"Sample ID":"S00017894",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012000",
"Sample ID":"S00017895",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012001",
"Sample ID":"S00017896",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012002",
"Sample ID":"S00017897",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0011995",
"Sample ID":"S00017898",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0011996",
"Sample ID":"S00017899",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0011997",
"Sample ID":"S00017900",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0011998",
"Sample ID":"S00017901",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0011999",
"Sample ID":"S00017902",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012000",
"Sample ID":"S00017903",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012001",
"Sample ID":"S00017904",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012002",
"Sample ID":"S00017905",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012003",
"Sample ID":"S00017906",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012004",
"Sample ID":"S00017907",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012005",
"Sample ID":"S00017908",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012006",
"Sample ID":"S00017909",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012007",
"Sample ID":"S00017910",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Bronchoalveolar Lavage Fluid"}
},
{
"Subject ID":"SU0012003",
"Sample ID":"S00017911",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012004",
"Sample ID":"S00017912",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012005",
"Sample ID":"S00017913",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012006",
"Sample ID":"S00017914",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012007",
"Sample ID":"S00017915",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012008",
"Sample ID":"S00017916",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n/a","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012009",
"Sample ID":"S00017917",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012010",
"Sample ID":"S00017918",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012011",
"Sample ID":"S00017919",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012012",
"Sample ID":"S00017920",
"Factors":{"Hyperoxia exposure":"n","RV infection":"n","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012013",
"Sample ID":"S00017921",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012014",
"Sample ID":"S00017922",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012015",
"Sample ID":"S00017923",
"Factors":{"Hyperoxia exposure":"y","RV infection":"n","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012016",
"Sample ID":"S00017924",
"Factors":{"Hyperoxia exposure":"y","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012017",
"Sample ID":"S00017925",
"Factors":{"Hyperoxia exposure":"n","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012018",
"Sample ID":"S00017926",
"Factors":{"Hyperoxia exposure":"n","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012019",
"Sample ID":"S00017927",
"Factors":{"Hyperoxia exposure":"n","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012020",
"Sample ID":"S00017928",
"Factors":{"Hyperoxia exposure":"n","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012021",
"Sample ID":"S00017929",
"Factors":{"Hyperoxia exposure":"y","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012022",
"Sample ID":"S00017930",
"Factors":{"Hyperoxia exposure":"y","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012023",
"Sample ID":"S00017931",
"Factors":{"Hyperoxia exposure":"y","RV infection":"y","Sample type":"Plasma"}
},
{
"Subject ID":"SU0012024",
"Sample ID":"S00017932",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012025",
"Sample ID":"S00017933",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012026",
"Sample ID":"S00017934",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012027",
"Sample ID":"S00017935",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012028",
"Sample ID":"S00017936",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012029",
"Sample ID":"S00017937",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012030",
"Sample ID":"S00017938",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012031",
"Sample ID":"S00017939",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012032",
"Sample ID":"S00017940",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
},
{
"Subject ID":"SU0012033",
"Sample ID":"S00017941",
"Factors":{"Hyperoxia exposure":"n/a","RV infection":"n/a","Sample type":"Trachea"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Bronchoalveolar Lavage Fluid"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"Glycolysis-TCA-nucleotides-20150324.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent 1260","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)","METHODS_FILENAME":"QQQ-ATP,ADP,AMP,A-update.m"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"Glycolysis-TCA-nucleotides-20150324.docx"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","INSTRUMENT_NAME":"Agilent 6410 QQQ Agilent QQQ 6410"},

"MS_METABOLITE_DATA":{
"Units":"nM",

"Data":[{"Metabolite":"Adenosine","S00017890":"451.21","S00017891":"384.48","S00017892":"145.60","S00017893":"343.71","S00017894":"410.64","S00017895":"321.17","S00017896":"186.06","S00017897":"3352.85","S00017898":"5587.76","S00017899":"4677.85","S00017900":"4444.76","S00017901":"2096.14","S00017902":"918.16","S00017903":"15609.60","S00017904":"4949.85","S00017905":"6778.91","S00017906":"3552.23","S00017907":"13413.11","S00017908":"5757.74","S00017909":"7643.50","S00017910":"4396.09","S00017911":"6657.44","S00017912":"6168.17","S00017913":"4545.65","S00017914":"10053.27","S00017915":"5467.71","S00017916":"5282.80","S00017917":"9597.40","S00017918":"182.04","S00017919":"92.67","S00017920":"112.08","S00017921":"210.36","S00017922":"235.64","S00017923":"60.15","S00017924":"93.10","S00017925":"203.92","S00017926":"194.00","S00017927":"79.69","S00017928":"1983.26","S00017929":"30936.99","S00017930":"11307.88","S00017931":"6061.51","S00017932":"26859.58","S00017933":"10789.11","S00017934":"16484.98","S00017935":"18779.74","S00017936":"359.24","S00017937":"1028.88","S00017938":"2906.41","S00017939":"2192.16","S00017940":"563.64","S00017941":"646.32"},{"Metabolite":"ADP","S00017890":"3667.65","S00017891":"1053.68","S00017892":"237.02","S00017893":"839.01","S00017894":"865.35","S00017895":"360.01","S00017896":"403.88","S00017897":"568.39","S00017898":"2564.21","S00017899":"937.09","S00017900":"1114.42","S00017901":"1588.48","S00017902":"720.34","S00017903":"640.46","S00017904":"415.69","S00017905":"178.73","S00017906":"319.00","S00017907":"175.97","S00017908":"249.84","S00017909":"195.50","S00017910":"715.78","S00017911":"123.47","S00017912":"86.93","S00017913":"59.43","S00017914":"322.01","S00017915":"746.51","S00017916":"143.26","S00017917":"106.24","S00017918":"102.85","S00017919":"99.25","S00017920":"67.46","S00017921":"73.84","S00017922":"76.37","S00017923":"68.86","S00017924":"90.89","S00017925":"77.31","S00017926":"88.77","S00017927":"65.28","S00017928":"2975.89","S00017929":"320.18","S00017930":"4393.75","S00017931":"3345.07","S00017932":"158.13","S00017933":"773.87","S00017934":"1917.18","S00017935":"383.45","S00017936":"2926.50","S00017937":"1848.17","S00017938":"3984.50","S00017939":"1491.40","S00017940":"1304.47","S00017941":"1610.76"},{"Metabolite":"AMP","S00017890":"4228.15","S00017891":"847.56","S00017892":"343.16","S00017893":"827.85","S00017894":"902.30","S00017895":"471.51","S00017896":"291.48","S00017897":"1517.69","S00017898":"5950.74","S00017899":"2386.09","S00017900":"2216.87","S00017901":"3228.80","S00017902":"868.85","S00017903":"4288.03","S00017904":"1466.39","S00017905":"1125.61","S00017906":"425.99","S00017907":"781.23","S00017908":"157.11","S00017909":"231.44","S00017910":"1843.49","S00017911":"214.76","S00017912":"165.44","S00017913":"94.30","S00017914":"1131.80","S00017915":"1325.26","S00017916":"109.08","S00017917":"143.13","S00017918":"69.79","S00017919":"206.26","S00017920":"69.68","S00017921":"60.13","S00017922":"63.08","S00017923":"89.38","S00017924":"365.93","S00017925":"90.79","S00017926":"78.92","S00017927":"64.68","S00017928":"4124.62","S00017929":"4864.90","S00017930":"10040.03","S00017931":"5640.95","S00017932":"2586.09","S00017933":"4839.49","S00017934":"11120.87","S00017935":"3644.17","S00017936":"4133.14","S00017937":"1742.97","S00017938":"12921.74","S00017939":"8149.03","S00017940":"1248.10","S00017941":"1094.87"},{"Metabolite":"ATP","S00017890":"4462.56","S00017891":"1617.21","S00017892":"416.06","S00017893":"2326.73","S00017894":"1076.46","S00017895":"442.57","S00017896":"629.87","S00017897":"1595.01","S00017898":"6917.69","S00017899":"2229.26","S00017900":"2811.10","S00017901":"4746.44","S00017902":"2463.69","S00017903":"353.09","S00017904":"0.00","S00017905":"174.14","S00017906":"355.67","S00017907":"199.79","S00017908":"404.81","S00017909":"230.96","S00017910":"626.81","S00017911":"238.20","S00017912":"99.81","S00017913":"73.11","S00017914":"254.52","S00017915":"484.64","S00017916":"227.81","S00017917":"0.00","S00017918":"145.63","S00017919":"143.58","S00017920":"0.00","S00017921":"139.97","S00017922":"121.98","S00017923":"93.74","S00017924":"143.57","S00017925":"0.00","S00017926":"182.33","S00017927":"0.00","S00017928":"11066.04","S00017929":"0.00","S00017930":"4408.58","S00017931":"5132.99","S00017932":"201.16","S00017933":"260.72","S00017934":"590.63","S00017935":"107.92","S00017936":"5141.16","S00017937":"2533.61","S00017938":"1577.94","S00017939":"467.35","S00017940":"1282.26","S00017941":"2703.69"}],

"Metabolites":[{"Metabolite":"Adenosine","PubChem CID":"60961"},{"Metabolite":"AMP","PubChem CID":"6083"},{"Metabolite":"ADP","PubChem CID":"6022"},{"Metabolite":"ATP","PubChem CID":"5957"}]
}

}