{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000344","ANALYSIS_ID":"AN000558","VERSION":"1","CREATED_ON":"February 12, 2016, 12:14 pm"},
"PROJECT":{"PROJECT_TITLE":"Effects of dietary supplement on hamster metabolism","PROJECT_SUMMARY":"This experiment aims to analyze spent media from a protein over-expression system. The treatment was a lipid supplement given to hamsters. The spent media was then analyzed to see how the lipid supplement affected lipid metabolism.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","FUNDING_SOURCE":"NIH U24DK097154"},
"STUDY":{"STUDY_TITLE":"Effects of dietary supplement on hamster metabolism","STUDY_SUMMARY":"This experiment aims to analyze spent media from a protein over-expression system. The treatment was a lipid supplement given to hamsters. The spent media was then analyzed to see how the lipid supplement affected lipid metabolism.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","NUM_GROUPS":"6","TOTAL_SUBJECTS":"28"},
"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mesocricetus auratus","TAXONOMY_ID":"10026"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"d2 C",
"Sample ID":"CHO cells_Elhofy_d2 C_001.d",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"d2 E",
"Sample ID":"CHO cells_Elhofy_d2 E_007.d",
"Factors":{"Treatment":"Lipid Supplement"}
},
{
"Subject ID":"d5 C",
"Sample ID":"CHO cells_Elhofy_d5 C_002.d",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"d5 E",
"Sample ID":"CHO cells_Elhofy_d5 E_008.d",
"Factors":{"Treatment":"Lipid Supplement"}
},
{
"Subject ID":"d8 C",
"Sample ID":"CHO cells_Elhofy_d8 C_003.d",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"d8 E",
"Sample ID":"CHO cells_Elhofy_d8 E_009.d",
"Factors":{"Treatment":"Lipid Supplement"}
},
{
"Subject ID":"d11 C",
"Sample ID":"CHO cells_Elhofy_d11 C_004.d",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"d11 E",
"Sample ID":"CHO cells_Elhofy_d11 E_010.d",
"Factors":{"Treatment":"Lipid Supplement"}
},
{
"Subject ID":"d14 C",
"Sample ID":"CHO cells_Elhofy_d14 C_005.d",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"d14 E",
"Sample ID":"CHO cells_Elhofy_d14 E_011.d",
"Factors":{"Treatment":"Lipid Supplement"}
},
{
"Subject ID":"d17 C",
"Sample ID":"CHO cells_Elhofy_d17 C_006.d",
"Factors":{"Treatment":"Control"}
},
{
"Subject ID":"d17 E",
"Sample ID":"CHO cells_Elhofy_d17 E_012.d",
"Factors":{"Treatment":"Lipid Supplement"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"This is spent media from a protein over-expression system. The media is serum free from the DG44 CHO cell line","COLLECTION_PROTOCOL_FILENAME":"StudyDesignAdamElhofy10202015.pdf"},
"TREATMENT":{"TREATMENT_SUMMARY":"2x6 each: The treatment is the addition of a lipid supplement.","TREATMENT_PROTOCOL_FILENAME":"StudyDesignAdamElhofy10202015.pdf"},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"None"},
"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"UPLC","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 6550","COLUMN_NAME":"Waters Acquity CSH C18 (100 x 2.1mm, 1.7um) 1.7um Pre-Column","FLOW_GRADIENT":"15% B to 99% B","FLOW_RATE":"0.6 mL/min","COLUMN_TEMPERATURE":"65 C","METHODS_FILENAME":"Data Dictionary Fiehn laboratory_CSH QTOF lipidomics_05-29-2014.pdf","SOLVENT_A":"60:40 Acetonitrile:Water +10mM Ammonium Acetate +10mM Acetic Acid","SOLVENT_B":"9:1 Isopropanol:Acetonitrile +10mM Ammonium Acetate +10mM Acetic Acid","COLUMN_PRESSURE":"450-850 bar","SAMPLE_INJECTION":"5.0 uL","ANALYTICAL_TIME":"13 min","CAPILLARY_VOLTAGE":"3500","TIME_PROGRAM":"15 min","WEAK_WASH_SOLVENT_NAME":"Isopropanol","WEAK_WASH_VOLUME":"5 seconds","STRONG_WASH_SOLVENT_NAME":"Same","TARGET_SAMPLE_TEMPERATURE":"Autosampler temp 4 C","RANDOMIZATION_ORDER":"Excel"},
"ANALYSIS":{"ANALYSIS_TYPE":"MS","DETECTOR_TYPE":"TOF MCP","SOFTWARE_VERSION":"MassHunter","DATA_FORMAT":".d"},
"MS":{"INSTRUMENT_NAME":"Agilent 6550 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Lipidomics runs are performed in negative and positve mode (two filenames)","CAPILLARY_VOLTAGE":"3500","COLLISION_GAS":"Nitrogen","DRY_GAS_FLOW":"13 l/min","DRY_GAS_TEMP":"200","FRAGMENT_VOLTAGE":"175","FRAGMENTATION_METHOD":"Auto MS/MS","ION_SOURCE_TEMPERATURE":"325","ION_SPRAY_VOLTAGE":"1000","IONIZATION":"Neg","PRECURSOR_TYPE":"Intact Molecule","REAGENT_GAS":"Nitrogen","SOURCE_TEMPERATURE":"325 C","DATAFORMAT":".d","DESOLVATION_GAS_FLOW":"11 l/min","DESOLVATION_TEMPERATURE":"350 C","NEBULIZER":"35 psig","OCTPOLE_VOLTAGE":"750","SCAN_RANGE_MOVERZ":"60-1700 Da","SCANNING_CYCLE":"2 Hz","SCANNING_RANGE":"60-1700 Da","SKIMMER_VOLTAGE":"65"},
"MS_METABOLITE_DATA":{
"Units":"Peak height",
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