{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000371","ANALYSIS_ID":"AN000605","VERSION":"1","CREATED_ON":"April 18, 2016, 5:27 pm"}, "PROJECT":{"PROJECT_TITLE":"Bacterial Regulators of Tumorigenesis","PROJECT_SUMMARY":"identify bacterial products associated with reduced tumorigenesis","INSTITUTE":"University of Michigan","DEPARTMENT":"Surgery","LABORATORY":"Lubman, David Lab","LAST_NAME":"Lubman","FIRST_NAME":"David","ADDRESS":"Ann Arbor, MI","EMAIL":"dmlubman@umich.edu","PHONE":"734-647-8834"}, "STUDY":{"STUDY_TITLE":"Colon cancer","STUDY_TYPE":"Regular","STUDY_SUMMARY":"There are 4 germfree controls and 4 germfree with bacteria. After treatment, the mouse stool samples were collected at different timepoints: day 0, day 10, day 26, day 47, and day 61 or 70. Place the stool samples into liquid nitrogen after they were collected, then stored in -80oC.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"11","TOTAL_SUBJECTS":"41"}, "SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"SU0012170", "Sample ID":"S00018140", "Factors":{"Time (day)":"-","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012170", "Sample ID":"S00018141", "Factors":{"Time (day)":"10","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012170", "Sample ID":"S00018142", "Factors":{"Time (day)":"26","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012170", "Sample ID":"S00018143", "Factors":{"Time (day)":"47","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012170", "Sample ID":"S00018144", "Factors":{"Time (day)":"61","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012171", "Sample ID":"S00018145", "Factors":{"Time (day)":"-","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012171", "Sample ID":"S00018146", "Factors":{"Time (day)":"10","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012171", "Sample ID":"S00018147", "Factors":{"Time (day)":"26","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012171", "Sample ID":"S00018148", "Factors":{"Time (day)":"47","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012171", "Sample ID":"S00018149", "Factors":{"Time (day)":"61","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012172", "Sample ID":"S00018150", "Factors":{"Time (day)":"-","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012173", "Sample ID":"S00018151", "Factors":{"Time (day)":"10","Treatment":"GF bacteria"} }, { "Subject ID":"SU0012173", "Sample ID":"S00018152", "Factors":{"Time (day)":"26","Treatment":"GF 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(day)":"-","Treatment":"none"} }, { "Subject ID":"SU0012178", "Sample ID":"S00018171", "Factors":{"Time (day)":"10","Treatment":"GF no bacteria"} }, { "Subject ID":"SU0012178", "Sample ID":"S00018172", "Factors":{"Time (day)":"26","Treatment":"GF no bacteria"} }, { "Subject ID":"SU0012178", "Sample ID":"S00018173", "Factors":{"Time (day)":"47","Treatment":"GF no bacteria"} }, { "Subject ID":"SU0012178", "Sample ID":"S00018174", "Factors":{"Time (day)":"70","Treatment":"GF no bacteria"} }, { "Subject ID":"SU0012179", "Sample ID":"S00018175", "Factors":{"Time (day)":"-","Treatment":"none"} }, { "Subject ID":"SU0012180", "Sample ID":"S00018176", "Factors":{"Time (day)":"10","Treatment":"GF no bacteria"} }, { "Subject ID":"SU0012180", "Sample ID":"S00018177", "Factors":{"Time (day)":"26","Treatment":"GF no bacteria"} }, { "Subject ID":"SU0012180", "Sample ID":"S00018178", "Factors":{"Time (day)":"47","Treatment":"GF no bacteria"} }, { "Subject ID":"SU0012180", "Sample ID":"S00018179", "Factors":{"Time (day)":"70","Treatment":"GF no bacteria"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"None","SAMPLE_TYPE":"Pooled sample"}, "TREATMENT":{"TREATMENT_SUMMARY":"none"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"none","SAMPLEPREP_PROTOCOL_FILENAME":"ShortChainFattyAcids-20150305.docx"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um)","COLUMN_TEMPERATURE":"WAXPLUS_100-200C_2_SIM.M.zip"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975C","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"}, "MS_METABOLITE_DATA":{ "Units":"nmol/mg sample", "Data":[{"Metabolite":"acetic 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