{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000371","ANALYSIS_ID":"AN000605","VERSION":"1","CREATED_ON":"April 18, 2016, 5:27 pm"},

"PROJECT":{"PROJECT_TITLE":"Bacterial Regulators of Tumorigenesis","PROJECT_SUMMARY":"identify bacterial products associated with reduced tumorigenesis","INSTITUTE":"University of Michigan","DEPARTMENT":"Surgery","LABORATORY":"Lubman, David Lab","LAST_NAME":"Lubman","FIRST_NAME":"David","ADDRESS":"Ann Arbor, MI","EMAIL":"dmlubman@umich.edu","PHONE":"734-647-8834"},

"STUDY":{"STUDY_TITLE":"Colon cancer","STUDY_TYPE":"Regular","STUDY_SUMMARY":"There are 4 germfree controls and 4 germfree with bacteria. After treatment, the mouse stool samples were collected at different timepoints: day 0, day 10, day 26, day 47, and day 61 or 70. Place the stool samples into liquid nitrogen after they were collected, then stored in -80oC.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"11","TOTAL_SUBJECTS":"41"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0012170",
"Sample ID":"S00018140",
"Factors":{"Time (day)":"-","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012170",
"Sample ID":"S00018141",
"Factors":{"Time (day)":"10","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012170",
"Sample ID":"S00018142",
"Factors":{"Time (day)":"26","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012170",
"Sample ID":"S00018143",
"Factors":{"Time (day)":"47","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012170",
"Sample ID":"S00018144",
"Factors":{"Time (day)":"61","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012171",
"Sample ID":"S00018145",
"Factors":{"Time (day)":"-","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012171",
"Sample ID":"S00018146",
"Factors":{"Time (day)":"10","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012171",
"Sample ID":"S00018147",
"Factors":{"Time (day)":"26","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012171",
"Sample ID":"S00018148",
"Factors":{"Time (day)":"47","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012171",
"Sample ID":"S00018149",
"Factors":{"Time (day)":"61","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012172",
"Sample ID":"S00018150",
"Factors":{"Time (day)":"-","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012173",
"Sample ID":"S00018151",
"Factors":{"Time (day)":"10","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012173",
"Sample ID":"S00018152",
"Factors":{"Time (day)":"26","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012173",
"Sample ID":"S00018153",
"Factors":{"Time (day)":"47","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012173",
"Sample ID":"S00018154",
"Factors":{"Time (day)":"61","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012174",
"Sample ID":"S00018155",
"Factors":{"Time (day)":"-","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012174",
"Sample ID":"S00018156",
"Factors":{"Time (day)":"10","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012174",
"Sample ID":"S00018157",
"Factors":{"Time (day)":"26","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012174",
"Sample ID":"S00018158",
"Factors":{"Time (day)":"47","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012174",
"Sample ID":"S00018159",
"Factors":{"Time (day)":"61","Treatment":"GF bacteria"}
},
{
"Subject ID":"SU0012175",
"Sample ID":"S00018160",
"Factors":{"Time (day)":"-","Treatment":"none"}
},
{
"Subject ID":"SU0012176",
"Sample ID":"S00018161",
"Factors":{"Time (day)":"10","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012176",
"Sample ID":"S00018162",
"Factors":{"Time (day)":"26","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012176",
"Sample ID":"S00018163",
"Factors":{"Time (day)":"47","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012176",
"Sample ID":"S00018164",
"Factors":{"Time (day)":"70","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012175",
"Sample ID":"S00018165",
"Factors":{"Time (day)":"-","Treatment":"none"}
},
{
"Subject ID":"SU0012177",
"Sample ID":"S00018166",
"Factors":{"Time (day)":"10","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012177",
"Sample ID":"S00018167",
"Factors":{"Time (day)":"26","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012177",
"Sample ID":"S00018168",
"Factors":{"Time (day)":"47","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012177",
"Sample ID":"S00018169",
"Factors":{"Time (day)":"70","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012175",
"Sample ID":"S00018170",
"Factors":{"Time (day)":"-","Treatment":"none"}
},
{
"Subject ID":"SU0012178",
"Sample ID":"S00018171",
"Factors":{"Time (day)":"10","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012178",
"Sample ID":"S00018172",
"Factors":{"Time (day)":"26","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012178",
"Sample ID":"S00018173",
"Factors":{"Time (day)":"47","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012178",
"Sample ID":"S00018174",
"Factors":{"Time (day)":"70","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012179",
"Sample ID":"S00018175",
"Factors":{"Time (day)":"-","Treatment":"none"}
},
{
"Subject ID":"SU0012180",
"Sample ID":"S00018176",
"Factors":{"Time (day)":"10","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012180",
"Sample ID":"S00018177",
"Factors":{"Time (day)":"26","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012180",
"Sample ID":"S00018178",
"Factors":{"Time (day)":"47","Treatment":"GF no bacteria"}
},
{
"Subject ID":"SU0012180",
"Sample ID":"S00018179",
"Factors":{"Time (day)":"70","Treatment":"GF no bacteria"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"None","SAMPLE_TYPE":"Pooled sample"},

"TREATMENT":{"TREATMENT_SUMMARY":"none"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"none","SAMPLEPREP_PROTOCOL_FILENAME":"ShortChainFattyAcids-20150305.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um)","COLUMN_TEMPERATURE":"WAXPLUS_100-200C_2_SIM.M.zip"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975C","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"nmol/mg sample",

"Data":[{"Metabolite":"acetic acid","S00018140":"5.67","S00018141":"3.93","S00018142":"4.72","S00018143":"4.71","S00018144":"6.57","S00018145":"4.55","S00018146":"3.88","S00018147":"3.40","S00018148":"3.46","S00018149":"5.68","S00018150":"5.26","S00018151":"3.90","S00018152":"4.33","S00018153":"4.04","S00018154":"4.90","S00018155":"5.24","S00018156":"3.37","S00018157":"4.19","S00018158":"4.53","S00018159":"6.11","S00018160":"1.87","S00018161":"2.07","S00018162":"2.21","S00018163":"1.90","S00018164":"1.96","S00018165":"1.98","S00018166":"1.92","S00018167":"1.85","S00018168":"1.93","S00018169":"1.85","S00018170":"1.73","S00018171":"1.84","S00018172":"2.00","S00018173":"2.07","S00018174":"1.85","S00018175":"1.80","S00018176":"1.80","S00018177":"1.80","S00018178":"1.92","S00018179":"1.73"},{"Metabolite":"propionic acid","S00018140":"7.51","S00018141":"5.83","S00018142":"6.70","S00018143":"6.42","S00018144":"8.31","S00018145":"6.21","S00018146":"5.97","S00018147":"5.69","S00018148":"5.79","S00018149":"7.39","S00018150":"7.00","S00018151":"6.05","S00018152":"6.44","S00018153":"6.37","S00018154":"6.62","S00018155":"6.97","S00018156":"5.96","S00018157":"6.62","S00018158":"6.79","S00018159":"8.15","S00018160":"4.47","S00018161":"4.65","S00018162":"4.79","S00018163":"4.60","S00018164":"4.55","S00018165":"4.76","S00018166":"4.81","S00018167":"4.63","S00018168":"4.66","S00018169":"4.58","S00018170":"4.49","S00018171":"4.52","S00018172":"4.67","S00018173":"4.90","S00018174":"4.63","S00018175":"4.55","S00018176":"4.61","S00018177":"4.64","S00018178":"4.64","S00018179":"4.48"},{"Metabolite":"iso-butyric acid","S00018140":"4.56","S00018141":"4.49","S00018142":"4.46","S00018143":"4.57","S00018144":"4.57","S00018145":"4.33","S00018146":"4.52","S00018147":"4.60","S00018148":"4.44","S00018149":"4.58","S00018150":"4.62","S00018151":"4.75","S00018152":"4.93","S00018153":"4.62","S00018154":"4.89","S00018155":"4.47","S00018156":"4.67","S00018157":"4.89","S00018158":"4.64","S00018159":"4.67","S00018160":"4.07","S00018161":"4.11","S00018162":"4.10","S00018163":"4.09","S00018164":"4.08","S00018165":"4.10","S00018166":"4.09","S00018167":"4.08","S00018168":"4.07","S00018169":"4.07","S00018170":"","S00018171":"4.07","S00018172":"","S00018173":"4.10","S00018174":"4.09","S00018175":"4.07","S00018176":"4.08","S00018177":"4.07","S00018178":"","S00018179":"4.07"},{"Metabolite":"butyric acid","S00018140":"2.42","S00018141":"2.40","S00018142":"2.38","S00018143":"2.36","S00018144":"2.60","S00018145":"2.34","S00018146":"2.43","S00018147":"2.34","S00018148":"2.39","S00018149":"2.55","S00018150":"2.40","S00018151":"2.41","S00018152":"2.36","S00018153":"2.47","S00018154":"2.44","S00018155":"2.42","S00018156":"2.36","S00018157":"2.35","S00018158":"2.73","S00018159":"2.67","S00018160":"2.25","S00018161":"2.26","S00018162":"2.27","S00018163":"2.25","S00018164":"2.26","S00018165":"2.26","S00018166":"2.26","S00018167":"2.25","S00018168":"2.26","S00018169":"2.26","S00018170":"2.25","S00018171":"2.21","S00018172":"2.27","S00018173":"2.26","S00018174":"2.26","S00018175":"2.25","S00018176":"2.25","S00018177":"2.25","S00018178":"2.26","S00018179":"2.25"},{"Metabolite":"isovaleric acid","S00018140":"2.42","S00018141":"2.40","S00018142":"2.38","S00018143":"2.36","S00018144":"2.60","S00018145":"2.34","S00018146":"2.43","S00018147":"2.34","S00018148":"2.39","S00018149":"2.55","S00018150":"2.40","S00018151":"2.41","S00018152":"2.36","S00018153":"2.47","S00018154":"2.44","S00018155":"2.42","S00018156":"2.36","S00018157":"2.35","S00018158":"2.73","S00018159":"2.67","S00018160":"2.25","S00018161":"2.26","S00018162":"2.27","S00018163":"2.25","S00018164":"2.26","S00018165":"2.26","S00018166":"2.26","S00018167":"2.25","S00018168":"2.26","S00018169":"2.26","S00018170":"2.25","S00018171":"2.21","S00018172":"2.27","S00018173":"2.26","S00018174":"2.26","S00018175":"2.25","S00018176":"2.25","S00018177":"2.25","S00018178":"2.26","S00018179":"2.25"},{"Metabolite":"valeric acid","S00018140":"2.05","S00018141":"2.01","S00018142":"2.03","S00018143":"2.03","S00018144":"2.03","S00018145":"2.00","S00018146":"1.99","S00018147":"2.01","S00018148":"1.99","S00018149":"2.03","S00018150":"2.03","S00018151":"2.02","S00018152":"2.08","S00018153":"2.01","S00018154":"2.05","S00018155":"2.05","S00018156":"2.02","S00018157":"2.04","S00018158":"2.00","S00018159":"2.03","S00018160":"1.93","S00018161":"1.93","S00018162":"1.93","S00018163":"1.93","S00018164":"1.93","S00018165":"1.93","S00018166":"1.93","S00018167":"1.93","S00018168":"1.94","S00018169":"1.93","S00018170":"1.93","S00018171":"1.93","S00018172":"1.93","S00018173":"1.94","S00018174":"1.93","S00018175":"1.93","S00018176":"1.93","S00018177":"1.93","S00018178":"1.95","S00018179":"1.93"},{"Metabolite":"hexanoic acid","S00018140":"3.00","S00018141":"3.00","S00018142":"3.00","S00018143":"3.01","S00018144":"3.01","S00018145":"3.00","S00018146":"3.00","S00018147":"3.00","S00018148":"3.00","S00018149":"3.01","S00018150":"3.00","S00018151":"3.00","S00018152":"3.00","S00018153":"3.00","S00018154":"3.00","S00018155":"3.00","S00018156":"3.00","S00018157":"3.00","S00018158":"3.01","S00018159":"3.02","S00018160":"2.99","S00018161":"2.99","S00018162":"3.00","S00018163":"3.00","S00018164":"2.99","S00018165":"3.00","S00018166":"3.00","S00018167":"2.99","S00018168":"2.99","S00018169":"2.99","S00018170":"2.99","S00018171":"2.99","S00018172":"3.00","S00018173":"2.99","S00018174":"2.99","S00018175":"2.99","S00018176":"2.99","S00018177":"2.99","S00018178":"2.99","S00018179":"2.99"},{"Metabolite":"heptanoic acid","S00018140":"3.09","S00018141":"3.15","S00018142":"3.19","S00018143":"3.24","S00018144":"3.14","S00018145":"3.13","S00018146":"3.12","S00018147":"3.12","S00018148":"3.15","S00018149":"3.10","S00018150":"3.15","S00018151":"3.11","S00018152":"3.11","S00018153":"3.13","S00018154":"3.11","S00018155":"3.13","S00018156":"3.13","S00018157":"3.10","S00018158":"3.13","S00018159":"3.10","S00018160":"3.11","S00018161":"3.18","S00018162":"3.14","S00018163":"3.10","S00018164":"3.14","S00018165":"3.11","S00018166":"3.11","S00018167":"3.09","S00018168":"3.10","S00018169":"3.11","S00018170":"3.11","S00018171":"3.11","S00018172":"3.12","S00018173":"3.12","S00018174":"3.10","S00018175":"3.13","S00018176":"3.14","S00018177":"3.14","S00018178":"3.11","S00018179":"3.12"}],

"Metabolites":[{"Metabolite":"acetic acid","retention index":"","quantified m/z":"","PubChem ID":"176","KEGG ID":""},{"Metabolite":"propionic acid","retention index":"","quantified m/z":"","PubChem ID":"1032","KEGG ID":""},{"Metabolite":"iso-butyric acid","retention index":"","quantified m/z":"","PubChem ID":"6590","KEGG ID":""},{"Metabolite":"butyric acid","retention index":"","quantified m/z":"","PubChem ID":"20975673","KEGG ID":""},{"Metabolite":"isovaleric acid","retention index":"","quantified m/z":"","PubChem ID":"10430","KEGG ID":""},{"Metabolite":"valeric acid","retention index":"","quantified m/z":"","PubChem ID":"7991","KEGG ID":""},{"Metabolite":"hexanoic acid","retention index":"","quantified m/z":"","PubChem ID":"8892","KEGG ID":""},{"Metabolite":"heptanoic acid","retention index":"","quantified m/z":"","PubChem ID":"8094","KEGG ID":""}]
}

}