{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000378","ANALYSIS_ID":"AN000612","VERSION":"1","CREATED_ON":"03-03-2022"},

"PROJECT":{"PROJECT_TITLE":"IDH1(R132H) mutations activity in neurospheres","PROJECT_SUMMARY":"assay 2HG procution in neurospheres expressing IDH1 mutated","INSTITUTE":"University of Michigan","DEPARTMENT":"Neurosurgery","LABORATORY":"Castro Lab","LAST_NAME":"Castro","FIRST_NAME":"Maria","ADDRESS":"Ann Arbor, MI","EMAIL":"mariacas@umich.edu","PHONE":"734-764-0850","DOI":"http://dx.doi.org/10.21228/M8NP5K"},

"STUDY":{"STUDY_TITLE":"2 hydroxiglutarate with with IDH1-R132H inhibitor in neurospheres","STUDY_TYPE":"Regular","STUDY_SUMMARY":"Tumor neurospheres carrying genetic lession NPI (NRAS, shP53, IDH1-R132H) colony 1 and 2 (C1 and C2); NPAI (NRAS, shP53, shATRX, IDH1-R132H) colony 1 and 2 (C1 and C2); and NPA (NRAS, shP53, shATRX) (C1 and C2). Was treated with IDH1-R132H inhibitor. By 2HG assay we want to check the effect of the inhibitor in our cells.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","SUBMIT_DATE":"2016-04-20"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0012404",
"Sample ID":"S00018624",
"Factors":{"shATRX plasmid":"no","IDH1-R132H mutation":"no"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018487",
"Factors":{"shATRX plasmid":"no","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018488",
"Factors":{"shATRX plasmid":"no","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018489",
"Factors":{"shATRX plasmid":"no","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018490",
"Factors":{"shATRX plasmid":"no","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012404",
"Sample ID":"S00018625",
"Factors":{"shATRX plasmid":"no","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018495",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"no"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018496",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"no"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018497",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"no"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018498",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"no"}
},
{
"Subject ID":"SU0012404",
"Sample ID":"S00018620",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"no"}
},
{
"Subject ID":"SU0012404",
"Sample ID":"S00018622",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"no"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018491",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018492",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018493",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012368",
"Sample ID":"S00018494",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012404",
"Sample ID":"S00018621",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"yes"}
},
{
"Subject ID":"SU0012404",
"Sample ID":"S00018623",
"Factors":{"shATRX plasmid":"yes","IDH1-R132H mutation":"yes"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"none","SAMPLE_TYPE":"Cell Line, Tumor"},

"TREATMENT":{"TREATMENT_SUMMARY":"none"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"none","SAMPLEPREP_PROTOCOL_FILENAME":"15DEC2015_2HG_cells_Castro.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"none","METHODS_ID":"DQM0033","METHODS_FILENAME":"2015-04-07-2HG.m.zip","INSTRUMENT_NAME":"None","COLUMN_NAME":"Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)","CHROMATOGRAPHY_TYPE":"Reversed phase"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"EX00452-2HG-QUANT-method.m.zip"},

"MS":{"INSTRUMENT_NAME":"Agilent 6410A QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"umol/g",

"Data":[{"Metabolite":"D-2-hydroxyglutarate","S00018487":"29.9000","S00018488":"17.6600","S00018489":"47.8500","S00018490":"9.9000","S00018495":"1.7300","S00018496":"1.1900","S00018497":"3.5000","S00018498":"2.7100","S00018491":"41.7300","S00018492":"11.7100","S00018493":"103.6700","S00018494":"20.9200"},{"Metabolite":"L-2-hydroxyglutarate","S00018487":"2.3700","S00018488":"4.0600","S00018489":"2.5200","S00018490":"2.0900","S00018495":"1.3100","S00018496":"0.8200","S00018497":"1.4400","S00018498":"1.3500","S00018491":"1.9000","S00018492":"1.4400","S00018493":"1.8200","S00018494":"1.7100"}],

"Metabolites":[{"Metabolite":"D-2-hydroxyglutarate","pubchem_id":"439391","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"L-2-hydroxyglutarate","pubchem_id":"439939","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""}]
}

}