{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000462","ANALYSIS_ID":"AN000723","VERSION":"1","CREATED_ON":"September 9, 2016, 12:13 pm"},

"PROJECT":{"PROJECT_TITLE":"CNS and peripheral metabolomics of calorie restriction in a mouse model of Alzheimer’s disease","PROJECT_SUMMARY":"Alzheimer’s disease (AD) is a devastating neurodegenerative disorder that robs people of their memory and cognitive function. Currently, no successful treatment or preventative measure exists for AD. Calorie restriction (CR) is a dietary regimen posited to suppress genetic programs of aging and reduce AD-related pathology. CR is known to enhance longevity and mitigate aging phenotypes in multiple model species. Mechanisms underlying the benefits of CR remain unknown, particularly in areas of the brain selectively vulnerable to age-related AD pathology such as the hippocampus, a region crucial for learning and memory. Moreover, AD pathology can be influenced by changes in diet, metabolism, and immunity, indicating that factors distant from the brain may play a role in pathogenesis. The intestinal microbiota, composed of trillions of microbial cells, influences host metabolism, immunity, and cognitive function, and is posited to be linked mechanistically to AD pathobiology, but a specific role remains to be adequately tested. We hypothesize that mechanisms underlying the benefits of CR are cell-type and organ specific, involving the gut-brain microbiome throughout the lifespan, this requiring subregional analysis in the brain as well as coordinated assessments of key peripheral targets including the liver, fecal pellets , and plasma. Thus, CR is proposed to be a viable treatment option that may ameliorate the development of AD-related pathology, and importantly, reveal mechanisms that attenuate age-related expression changes in vulnerable cells.","INSTITUTE":"New York University","DEPARTMENT":"Langone Medical Center","LAST_NAME":"Ginsberg","FIRST_NAME":"Stephen","ADDRESS":"140 Old Orangeburg Road, Orangeburg, NY 10962","EMAIL":"stephen.ginsberg@nyumc.org","PHONE":"845-398-2170"},

"STUDY":{"STUDY_TITLE":"CNS and peripheral metabolomics of calorie restriction in a mouse model of Alzheimer’s disease (part I)","STUDY_SUMMARY":"This pilot metabolomic study will evaluate brain specimens from an established mouse model of AD, the tq2576 mouse model of cerebral amyloid overexpression (APP), in comparison to their non-transgenic (NTG) littermates. These animals were either on a CR or ad libitum (AL) diet, and specimens were collected at two time points (5 and 15 months of age). Tissue from this cohorts of mice have already undergone microbiome analysis, and await coordinated brain and peripheral tissue assessments. Future analysis will include metabolomics, RNA-seq, and microarray data to assess the gut-brain microbiome system in neurodegenerative disorders.","INSTITUTE":"RTI International","LAST_NAME":"Sumner","FIRST_NAME":"Susan","ADDRESS":"3040 E. Cornwallis Road","EMAIL":"ssumner@rti.org","PHONE":"919-541-7479"},

"SUBJECT":{"SUBJECT_TYPE":"Murine","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
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{
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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{
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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{
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{
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"Additional sample data":{"Sample":"Sample"}
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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{
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Sample":"Sample"}
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{
"Subject ID":"-",
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"Additional sample data":{"Sample":"Sample"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Sample":"Sample"}
},
{
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"Additional sample data":{"Sample":"Sample"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Sample":"Sample"}
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{
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"Additional sample data":{"Sample":"Sample"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Brain (without hippocampus and entorhinal cortex)"},

"TREATMENT":{"TREATMENT_SUMMARY":"Calorie restricted (CR) and ad libitum (AL) diets"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Brain tissue samples were homogenized in 50:50 Acetonitrile:Water , and the final concentration of all of the extracts was 1 mL/mg. Of the 71 samples, 48 had sufficient volume for study samples and for an analytical quality control (QC) total pool sample. This total pool was generated by transferring a 50 µL of each sample and aliquoting into six replicates, which were processed identically to the study samples. A 250 µL aliquot of each sample homogenate was lyophilized and then reconstituted with 250 µL of Phosphate buffer with Chenomx ISTD and 6mM Imidazole. Samples were vortexed and centrifuged before transferring 225 µL of each sample into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR"},

"NM":{"INSTRUMENT_NAME":"Bruker Avance III","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","SPECTROMETER_FREQUENCY":"700.13 MHz"},

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