{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000498","ANALYSIS_ID":"AN000766","VERSION":"1","CREATED_ON":"October 31, 2016, 10:41 am"}, "PROJECT":{"PROJECT_TITLE":"Drug's effect on cerebral metabolic pool sizes","PROJECT_SUMMARY":"The goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to drug administration and withdrawal","INSTITUTE":"University of Florida","DEPARTMENT":"Biochemistry and Molecular Biology","LAST_NAME":"Joanna","FIRST_NAME":"Long","ADDRESS":"1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611","EMAIL":"jrlong@mbi.ufl.edu","PHONE":"none","FUNDING_SOURCE":"UF"}, "STUDY":{"STUDY_TITLE":"Cocaine administration and withdrawal","STUDY_TYPE":"treated and control","STUDY_SUMMARY":"The goal of this project is to analyze the metabolic pool distribution of cerebral metabolites in response to cocaine administration and withdrawal as compared to control","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LAST_NAME":"Daniel","FIRST_NAME":"Downes","ADDRESS":"1149 Newell Drive BLDG 59 LG-129 Gainesville, FL 32611","EMAIL":"daniel.downes@neurosurgery.ufl.edu","PHONE":"(321)303-6523","NUM_GROUPS":"5","TOTAL_SUBJECTS":"12"}, "SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Rattus norvegicus","TAXONOMY_ID":"10116","SUBJECT_COMMENTS":"Long Evans Rat"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"LongEvans1", "Sample ID":"LongDNP_001", "Factors":{"drug admin":"acute"}, "Additional sample data":{"body weight":"339g"} }, { "Subject ID":"LongEvans2", "Sample ID":"LongDNP_002", "Factors":{"drug admin":"acute"}, "Additional sample data":{"body weight":"402g"} }, { "Subject ID":"LongEvans3", "Sample ID":"LongDNP_003", "Factors":{"drug admin":"acute"}, "Additional sample data":{"body weight":"363g"} }, { "Subject ID":"LongEvans4", "Sample ID":"LongDNP_004", "Factors":{"drug admin":"acute control"}, "Additional sample data":{"body weight":"425g"} }, { "Subject ID":"LongEvans5", "Sample ID":"LongDNP_005", "Factors":{"drug admin":"acute control"}, "Additional sample data":{"body weight":"380g"} }, { "Subject ID":"LongEvans6", "Sample ID":"LongDNP_006", "Factors":{"drug admin":"acute control"}, "Additional sample data":{"body weight":"397g"} }, { "Subject ID":"LongEvans7", "Sample ID":"LongDNP_007", "Factors":{"drug admin":"withdrawal"}, "Additional sample data":{"body weight":"447g"} }, { "Subject ID":"LongEvans8", "Sample ID":"LongDNP_008", "Factors":{"drug admin":"withdrawal"}, "Additional sample data":{"body weight":"375g"} }, { "Subject ID":"LongEvans9", "Sample ID":"LongDNP_009", "Factors":{"drug admin":"withdrawal"}, "Additional sample data":{"body weight":"373g"} }, { "Subject ID":"LongEvans10", "Sample ID":"LongDNP_010", "Factors":{"drug admin":"withdrawal control"}, "Additional sample data":{"body weight":"385g"} }, { "Subject ID":"LongEvans11", "Sample ID":"LongDNP_011", "Factors":{"drug admin":"withdrawal control"}, "Additional sample data":{"body weight":"407g"} }, { "Subject ID":"LongEvans12", "Sample ID":"LongDNP_012", "Factors":{"drug admin":"withdrawal control"}, "Additional sample data":{"body weight":"434g"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"harvested, rinsed with cold saline, left hemisphere flash frozen in liquid N2","SAMPLE_TYPE":"Tissue"}, "TREATMENT":{"TREATMENT_SUMMARY":"Acute injection = 10mg/kg/cc cocaine i.p, Acute Control = no injection, Withdrawal = 10mg/kg/cc cocaine i.p daily for 10 days_3days withdrawal, Withdrawal control = 1ml/kg saline i.p daily for 10 days_3 days withdrawal"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"none","SAMPLEPREP_PROTOCOL_FILENAME":"SB_OA_Assay.pdf"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Quantiva QQQ","COLUMN_NAME":"Waters Acquity BEH C18 (100 x 2mm, 1.7um)","METHODS_FILENAME":"SB_OA_Assay.pdf"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Sanford Burnham Prebys"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo Quantiva QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE"}, "MS_METABOLITE_DATA":{ "Units":"nmol/mg dry powder", "Data":[{"Metabolite":"Lactate","LongDNP_004":"58.99","LongDNP_005":"61.21","LongDNP_006":"57.85","LongDNP_001":"67.32","LongDNP_002":"73.85","LongDNP_003":"62.09","LongDNP_010":"54.93","LongDNP_011":"65.42","LongDNP_012":"60.16","LongDNP_007":"46.36","LongDNP_008":"50.16","LongDNP_009":"65.27"},{"Metabolite":"Pyruvate","LongDNP_004":"0.07","LongDNP_005":"0.07","LongDNP_006":"0.07","LongDNP_001":"0.08","LongDNP_002":"0.08","LongDNP_003":"0.09","LongDNP_010":"0.06","LongDNP_011":"0.05","LongDNP_012":"0.07","LongDNP_007":"0.06","LongDNP_008":"0.06","LongDNP_009":"0.07"},{"Metabolite":"Succinate","LongDNP_004":"7.17","LongDNP_005":"1.80","LongDNP_006":"2.37","LongDNP_001":"2.17","LongDNP_002":"2.12","LongDNP_003":"1.96","LongDNP_010":"2.04","LongDNP_011":"2.64","LongDNP_012":"1.83","LongDNP_007":"2.13","LongDNP_008":"1.90","LongDNP_009":"2.21"},{"Metabolite":"Fumarate","LongDNP_004":"0.14","LongDNP_005":"0.15","LongDNP_006":"0.16","LongDNP_001":"0.18","LongDNP_002":"0.16","LongDNP_003":"0.16","LongDNP_010":"0.16","LongDNP_011":"0.10","LongDNP_012":"0.13","LongDNP_007":"0.19","LongDNP_008":"0.14","LongDNP_009":"0.17"},{"Metabolite":"Malate","LongDNP_004":"0.80","LongDNP_005":"1.03","LongDNP_006":"1.13","LongDNP_001":"1.18","LongDNP_002":"1.02","LongDNP_003":"1.10","LongDNP_010":"1.11","LongDNP_011":"0.66","LongDNP_012":"0.89","LongDNP_007":"1.26","LongDNP_008":"1.05","LongDNP_009":"1.17"},{"Metabolite":"a-KG","LongDNP_004":"0.02","LongDNP_005":"0.01","LongDNP_006":"0.01","LongDNP_001":"0.02","LongDNP_002":"0.01","LongDNP_003":"0.02","LongDNP_010":"0.01","LongDNP_011":"0.01","LongDNP_012":"0.01","LongDNP_007":"0.01","LongDNP_008":"0.01","LongDNP_009":"0.02"},{"Metabolite":"Citrate","LongDNP_004":"0.37","LongDNP_005":"0.53","LongDNP_006":"0.49","LongDNP_001":"0.55","LongDNP_002":"0.60","LongDNP_003":"0.51","LongDNP_010":"0.51","LongDNP_011":"0.30","LongDNP_012":"0.48","LongDNP_007":"0.59","LongDNP_008":"0.40","LongDNP_009":"0.51"}], "Metabolites":[{"Metabolite":"Lactate","retention index":"3.98","quantified m/z":"196.1, 91.062","PubChem ID":"107689","KEGG ID":""},{"Metabolite":"Pyruvate","retention index":"6.68","quantified m/z":"299.1, 91.186","PubChem ID":"1060","KEGG ID":""},{"Metabolite":"Succinate","retention index":"5.44","quantified m/z":"329.1, 206.233","PubChem ID":"1110","KEGG ID":""},{"Metabolite":"Fumarate","retention index":"5.59","quantified m/z":"327.1, 91.192","PubChem ID":"444972","KEGG ID":""},{"Metabolite":"Malate","retention index":"5.28","quantified m/z":"345.1, 91.18","PubChem ID":"525","KEGG ID":""},{"Metabolite":"a-KG","retention index":"6.59","quantified m/z":"462.2, 91.2","PubChem ID":"164533","KEGG ID":""},{"Metabolite":"Citrate","retention index":"5.95","quantified m/z":"508.25, 385","PubChem ID":"311","KEGG ID":""}] } }