{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000558","ANALYSIS_ID":"AN000856","VERSION":"1","CREATED_ON":"02-08-2024"},

"PROJECT":{"PROJECT_TITLE":"Plasma metabolomic profiling of diabetic nephropathy in the steptozotocin induced type-1 diabetes mouse model","PROJECT_SUMMARY":"Diabetic nephropathy (DN) is the leading cause of end stage renal disease, and is associated with high morbidity and mortality rates. The pathophysiology of DN includes both glomerular and tubulointerstitial damage. Meprins are metalloproteinases which are most abundantly expressed in the brush border membranes of proximal kidney tubules. Meprins are also expressed in leukocytes (monocytes and macrophages) and podocytes. Meprins have been implicated in the pathology of acute and chronic kidney injury. Single nucleotide polymorphisms (SNPs) in the meprin β gene were associated in human DN in the Pima Indians, suggesting a role for meprins in the pathophysiology of DN. The current study was done to determine the mechanisms by which meprins modulate the progression of DN in mice.","INSTITUTE":"North Carolina A&T State University","LAST_NAME":"Ongeri","FIRST_NAME":"Elimelda Moige","ADDRESS":"1601 E Market Street, Greensboro, NC 27411","EMAIL":"eongeri@ncat.edu","PHONE":"336-285-2182","FUNDING_SOURCE":"NIH/NIGMS Grant # SC3102049 To Elimelda Moige Ongeri; NIH Center Grant # U24DK097193 to Susan Sumner; NIH/NCATS award # UL1TR001111 to John Buse, UNC-CH, PI, Sumner- Director, Metabolomics core; and NIH/NIGMS Grant # K01GM109320 to Jessica Gooding.","DOI":"http://dx.doi.org/10.21228/M8602H"},

"STUDY":{"STUDY_TITLE":"Plasma metabolomic profiling of diabetic nephropathy in the steptozotocin induced type-1 diabetes mouse model","STUDY_SUMMARY":"This metabolomics study evaluated plasma from wild-type and meprin β knockout mice after induction of diabetes with streptozotocin or treatment with sodium citrate control to understand how these factors influence the metabotype.","INSTITUTE":"RTI International","LAST_NAME":"Sumner","FIRST_NAME":"Susan","ADDRESS":"3040 E. Cornwallis Road, Research Triangle Park, NC 27709","EMAIL":"susan_sumner@unc.edu","PHONE":"704-250-5000","SUBMIT_DATE":"2017-02-17"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"KO: bred and housed at the laboratory animal resource unit (LARU) of North Carolina A&T State University; WT: Charles River Laboratories","ANIMAL_HOUSING":"group cages of up to 5 mice per cage","ANIMAL_LIGHT_CYCLE":"12:12h light:dark cycle","ANIMAL_FEED":"rat chow","ANIMAL_WATER":"water ad libitum","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Equilibrium_6",
"Sample ID":"Equilibrium_6",
"Factors":{"Treatment":"-","Genotype":"-","Week":"-"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P_Equil6_POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P_Equil6_NEG.raw","Gender":"-"}
},
{
"Subject ID":"Total Pool_2",
"Sample ID":"Total Pool_2",
"Factors":{"Treatment":"-","Genotype":"-","Week":"-"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P_Pool_2_POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P_Pool_2_NEG.raw","Gender":"-"}
},
{
"Subject ID":"Total Pool_3",
"Sample ID":"Total Pool_3",
"Factors":{"Treatment":"-","Genotype":"-","Week":"-"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P_Pool_3_POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P_Pool_3_NEG.raw","Gender":"-"}
},
{
"Subject ID":"Total Pool_4",
"Sample ID":"Total Pool_4",
"Factors":{"Treatment":"-","Genotype":"-","Week":"-"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P_Pool_4_POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P_Pool_4_NEG.raw","Gender":"-"}
},
{
"Subject ID":"Total Pool_5",
"Sample ID":"Total Pool_5",
"Factors":{"Treatment":"-","Genotype":"-","Week":"-"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P_Pool_5_POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P_Pool_5_NEG.raw","Gender":"-"}
},
{
"Subject ID":"Total Pool_6",
"Sample ID":"Total Pool_6",
"Factors":{"Treatment":"-","Genotype":"-","Week":"-"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P_Pool_6 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P_Pool_6 _NEG.raw","Gender":"-"}
},
{
"Subject ID":"383",
"Sample ID":"383-4",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_NaC_383 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_NaC_383 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"387",
"Sample ID":"387-4",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_NaC_387 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_NaC_387 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"390",
"Sample ID":"390-4",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_NaC_390 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_NaC_390 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"391",
"Sample ID":"391-4",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_NaC_391 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_NaC_391 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"398",
"Sample ID":"398-4",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_NaC_398 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_NaC_398 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"399",
"Sample ID":"399-4",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_NaC_399 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_NaC_399 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"383",
"Sample ID":"383-8",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_NaC_383 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_NaC_383 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"387",
"Sample ID":"387-8",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_NaC_387 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_NaC_387 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"390",
"Sample ID":"390-8",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_NaC_390 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_NaC_390 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"391",
"Sample ID":"391-8",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_NaC_391 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_NaC_391 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"398",
"Sample ID":"398-8",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_NaC_398 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_NaC_398 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"399",
"Sample ID":"399-8",
"Factors":{"Treatment":"NaC","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_NaC_399 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_NaC_399 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"519",
"Sample ID":"519-4",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_NaC_519 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_NaC_519 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"520",
"Sample ID":"520-4",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_NaC_520 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_NaC_520 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"521",
"Sample ID":"521-4",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_NaC_521 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_NaC_521 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"522",
"Sample ID":"522-4",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_NaC_522 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_NaC_522 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"523",
"Sample ID":"523-4",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_NaC_523 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_NaC_523 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"519",
"Sample ID":"519-8",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_NaC_519 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_NaC_519 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"520",
"Sample ID":"520-8",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_NaC_520 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_NaC_520 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"521",
"Sample ID":"521-8",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_NaC_521 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_NaC_521 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"522",
"Sample ID":"522-8",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_NaC_522 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_NaC_522 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"523",
"Sample ID":"523-8",
"Factors":{"Treatment":"NaC","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_NaC_523 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_NaC_523 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"395",
"Sample ID":"395-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_395 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_STZ_395 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"396",
"Sample ID":"396-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_396 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_STZ_396 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"397",
"Sample ID":"397-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_397 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_STZ_397 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"400",
"Sample ID":"400-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_400 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_STZ_400 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"514",
"Sample ID":"514-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_514 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_STZ_514 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"515",
"Sample ID":"515-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_515 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"-","Gender":"Male"}
},
{
"Subject ID":"516",
"Sample ID":"516-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_516 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_STZ_516 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"518",
"Sample ID":"518-4",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_KO_STZ_518 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_KO_STZ_518 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"395",
"Sample ID":"395-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_395 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_395 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"396",
"Sample ID":"396-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_396 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_396 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"397",
"Sample ID":"397-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_397 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_397 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"400",
"Sample ID":"400-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_400 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_400 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"514",
"Sample ID":"514-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_514 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_514 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"515",
"Sample ID":"515-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_515 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_515 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"516",
"Sample ID":"516-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_516 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_516 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"518",
"Sample ID":"518-8",
"Factors":{"Treatment":"STZ","Genotype":"Meprin bKO","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_KO_STZ_518 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_KO_STZ_518 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"525",
"Sample ID":"525-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_525 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_STZ_525 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"526",
"Sample ID":"526-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_526 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_STZ_526 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"527",
"Sample ID":"527-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_527 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"-","Gender":"Male"}
},
{
"Subject ID":"528",
"Sample ID":"528-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_528 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_STZ_528 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"529",
"Sample ID":"529-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_529 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_STZ_529 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"530",
"Sample ID":"530-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_530 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_STZ_530 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"531",
"Sample ID":"531-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_531 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_STZ_531 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"532",
"Sample ID":"532-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_532 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"-","Gender":"Male"}
},
{
"Subject ID":"533",
"Sample ID":"533-4",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"4"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P4_WT_STZ_533 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P4_WT_STZ_533 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"525",
"Sample ID":"525-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_525 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_STZ_525 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"526",
"Sample ID":"526-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_526 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_STZ_526 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"528",
"Sample ID":"528-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_528 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"-","Gender":"Male"}
},
{
"Subject ID":"529",
"Sample ID":"529-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_529 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_STZ_529 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"530",
"Sample ID":"530-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_530 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_STZ_530 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"531",
"Sample ID":"531-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_531 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_STZ_531 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"532",
"Sample ID":"532-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_532 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_STZ_532 _NEG.raw","Gender":"Male"}
},
{
"Subject ID":"533",
"Sample ID":"533-8",
"Factors":{"Treatment":"STZ","Genotype":"wild-type","Week":"8"},
"Additional sample data":{"Sample Identifer Instrument Run Name (POS)":"MO_P8_WT_STZ_533 _POS.raw","Sample Identifier Instrument Run Name (NEG)":"MO_P8_WT_STZ_533 _NEG.raw","Gender":"Male"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Blood samples were collected at 4 and 8 weeks post-STZ injection. Blood was obtained by tail nicking at week 4 and cardiac puncture at week 8, collected into heparin tubes, centrifuged to obtain plasma, and stored at -80oC until used.","SAMPLE_TYPE":"Blood","STORAGE_CONDITIONS":"-80 C","ADDITIVES":"Heparin","BLOOD_SERUM_OR_PLASMA":"Plasma"},

"TREATMENT":{"TREATMENT_SUMMARY":"Type 1 diabetes was induced at age 8 weeks. The mice were fasted for 6 hours prior to being injected with low dose STZ (50 mg/kg body weight) in sodium citrate buffer (10 mmol/L, pH 4.5) daily for 5 days following the protocols described by Tesch and Allen and recommended by the Animal Models of Diabetic Complications Consortium (AMDCC; amdcc.org)1. Control mice were injected with equivalent volumes of the sodium citrate buffer. Diabetes was confirmed by measuring fasting blood glucose levels using a standard glucose meter at 10 days post-STZ injection. Mice with a fasting glucose level ≥280 mg/dL were considered diabetic. STZ-injected mice with a fasting blood glucose of <280 mg/dL (15mmol/L) were culled and eliminated from the study.","TREATMENT_COMPOUND":"streptozotocin","TREATMENT_DOSE":"50 mg/kg body weight","TREATMENT_DOSEDURATION":"daily; 5 days","TREATMENT_VEHICLE":"sodium citrate","ANIMAL_ENDP_TISSUE_COLL_LIST":"plasma, urine, kidney tissue"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Plasma samples were thawed on ice for 30–60 min and vortexed on a multi-tube vortexer for 4 min at 5,000 rpm, followed by centrifugation at room temperature at 16,000 rcf for 4 min. An aliquot of the supernatant was transferred to pre-labeled 1.5 mL low protein-binding microcentrifuge tube to make the individual study sample (40 µL). Another aliquot of supernatant (11 µL) was combined with those from all other plasma samples in a 1.5 mL low protein-binding microcentrifuge tube to make the total pool quality control sample. The total pool quality control sample was aliquoted (40 µL) to make 6 Total Pool Samples and 4 Column Equilibration samples. 132 µL of the total pool quality control sample was added to the total study sample pool to make a pool of kidney, urine, and plasma samples. The total study pool sample was used to prepare 9 Total Study Samples (40 µL) to aid in alignment of the three studies. Of these, 3 total study samples were included in the plasma analysis. Methanol containing the internal standard Tryptophan-d5 (320 µL; 0.0125 mg/ml) was added to all samples, and samples were vortexed on a multiple tube vortexer for 2 min at 5000 rpm followed by centrifugation at 16,000 rcf for 4 min. The supernatants (290 µL) were transferred into new, pre-labeled 2.0 mL low protein-binding microcentrifuge tubes and capped with disposable rubber stoppers. Samples were placed at -80 °C for 1 h and lyophilized for 18 h. Acetonitrile:Water (125 µL; 95:5) was added to each tube and vortex mixed for 10 min at 5000 rpm, followed by centrifugation at room temperature at 16,000 rcf for 4 min. The supernatants were transferred to pre-labeled autosampler vials and 3 µL was injected into SYNAPT G2-Si."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Hydrophilic Interaction Liquid Chromatography (HILIC) Gradient Seperation","INSTRUMENT_NAME":"Waters Acquity I-Class","COLUMN_NAME":"Waters Acquity BEH Amide (150 x 2.1mm,1.7um)","CHROMATOGRAPHY_TYPE":"HILIC"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Waters Synapt G2 Si QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE"}

}