{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000723","ANALYSIS_ID":"AN001133","VERSION":"1","CREATED_ON":"July 2, 2017, 9:44 pm"},

"PROJECT":{"PROJECT_TITLE":"Analysis of SCFA in the fecal matter of wt and Orai1kO mice","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Orai1 mediated pancreatic secretion is critical for gut innate immunity","INSTITUTE":"National Institutes of Health","DEPARTMENT":"NIDCR/MPTB","LABORATORY":"Muallem Lab","LAST_NAME":"Muallem","FIRST_NAME":"Shmuel","ADDRESS":"Bethesda, Maryland","EMAIL":"301-402-0262","PHONE":"301-471-3027"},

"STUDY":{"STUDY_TITLE":"Analysis of SCFA in the fecal matter of wt and Orai1kO mice","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"The gut microbiome participates in numerous physiological functions and is believed to be shaped by the host intestine. We report here an unsuspected prominent role of Orai1-mediated pancreatic acinar cell exocytosis in shaping the microbiome. Deletion of Orai1 in pancreatic acini of mature mice maintained on solid diet resulted in 60-70% mortality. The mice have severe bacterial burden with dysbiosis resulting in systemic inflammation and death. This occurs despite prominent Paneth cell hyperplasia and activation of the intestinal innate immune response. Secretion of and bacterial killing by pancreatic defensins are markedly reduced, resulting in microbiota with decreased diversity and increased pathogenic bacteria. Survival and weight gain are not rescued by digestive enzyme supplements, but are by broad-spectrum antibiotics and purified liquid diet. These findings reveal an unexpected profound role of pancreatic secretion in shaping the microbiome","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"4","TOTAL_SUBJECTS":"4"},

"SUBJECT":{"SUBJECT_TYPE":"MOUSE","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0016351",
"Sample ID":"S00026161",
"Factors":{"Diet":"solid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016351",
"Sample ID":"S00026162",
"Factors":{"Diet":"solid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016351",
"Sample ID":"S00026163",
"Factors":{"Diet":"solid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016351",
"Sample ID":"S00026164",
"Factors":{"Diet":"solid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016351",
"Sample ID":"S00026165",
"Factors":{"Diet":"solid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016351",
"Sample ID":"S00026166",
"Factors":{"Diet":"solid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016352",
"Sample ID":"S00026167",
"Factors":{"Diet":"solid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016352",
"Sample ID":"S00026168",
"Factors":{"Diet":"solid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016352",
"Sample ID":"S00026169",
"Factors":{"Diet":"solid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016352",
"Sample ID":"S00026170",
"Factors":{"Diet":"solid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016352",
"Sample ID":"S00026171",
"Factors":{"Diet":"solid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016352",
"Sample ID":"S00026172",
"Factors":{"Diet":"solid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016353",
"Sample ID":"S00026173",
"Factors":{"Diet":"liquid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016353",
"Sample ID":"S00026174",
"Factors":{"Diet":"liquid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016353",
"Sample ID":"S00026175",
"Factors":{"Diet":"liquid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016353",
"Sample ID":"S00026176",
"Factors":{"Diet":"liquid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016353",
"Sample ID":"S00026177",
"Factors":{"Diet":"liquid diet","Genotype":"wt"}
},
{
"Subject ID":"SU0016354",
"Sample ID":"S00026178",
"Factors":{"Diet":"liquid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016354",
"Sample ID":"S00026179",
"Factors":{"Diet":"liquid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016354",
"Sample ID":"S00026180",
"Factors":{"Diet":"liquid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016354",
"Sample ID":"S00026181",
"Factors":{"Diet":"liquid diet","Genotype":"Orai1Ko"}
},
{
"Subject ID":"SU0016354",
"Sample ID":"S00026182",
"Factors":{"Diet":"liquid diet","Genotype":"Orai1Ko"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A007 - Short Chain Fatty Acids.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890N","COLUMN_NAME":"Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A007 - Short Chain Fatty Acids.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"nmol/mg",

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}

}