{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000736","ANALYSIS_ID":"AN001150","VERSION":"1","CREATED_ON":"July 3, 2017, 12:32 pm"},

"PROJECT":{"PROJECT_TITLE":"Metabolic change in macrophages after efferocytosis","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"To study metabolic change in macrophages after efferocytosis","INSTITUTE":"Northwestern University","DEPARTMENT":"Pathology","LABORATORY":"Thorp Lab","LAST_NAME":"Thorp","FIRST_NAME":"Edward","ADDRESS":"Evanston, IL","EMAIL":"ebthorp@northwestern.edu","PHONE":"312-503-4309"},

"STUDY":{"STUDY_TITLE":"Metabolic change in macrophages after efferocytosis","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"Study metabolic pathway alteration in macrophages after efferocytosis (when macrophages eating apoptotic cells). Peritoneal macrophages were purified and plated in the cell-culture dishes. After culturing overnight. Apoptotic cells were overlayed to macrophages for 2 hours, then un-engulfed cells were removed through washing. Macrophages were left in the plate for another 4 hours before being lifted from the plate.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"5","TOTAL_SUBJECTS":"1"},

"SUBJECT":{"SUBJECT_TYPE":"Mouse","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0013786",
"Sample ID":"S00020913",
"Factors":{"Treatment":"none"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020914",
"Factors":{"Treatment":"none"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020915",
"Factors":{"Treatment":"apoptotic cells for 2 hours"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020916",
"Factors":{"Treatment":"apoptotic cells for 2 hours"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020917",
"Factors":{"Treatment":"apoptotic cells for 2 hours"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020918",
"Factors":{"Treatment":"ko untreated"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020919",
"Factors":{"Treatment":"ko untreated"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020920",
"Factors":{"Treatment":"ko treated with apoptotic cells 2hrs"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020921",
"Factors":{"Treatment":"ko treated with apoptotic cells 2 hrs"}
},
{
"Subject ID":"SU0013786",
"Sample ID":"S00020922",
"Factors":{"Treatment":"ko treated with apoptotic cells 2 hrs"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A011 - Glycolysis-TCA-nucleotides.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A011 - Glycolysis-TCA-nucleotides.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6520B QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"counts",

"Data":[{"Metabolite":"XANTHINE","S00020913":"105151","S00020914":"63757","S00020915":"579974","S00020916":"578715","S00020917":"535447","S00020918":"340657","S00020919":"373986","S00020920":"780294","S00020921":"874081","S00020922":"642803"},{"Metabolite":"HEXOPYRANOSE","S00020913":"259377","S00020914":"341640","S00020915":"666318","S00020916":"573966","S00020917":"596058","S00020918":"272018","S00020919":"252238","S00020920":"791756","S00020921":"725652","S00020922":"771341"},{"Metabolite":"CITRIC ACID","S00020913":"8583517","S00020914":"6624953","S00020915":"7639874","S00020916":"5929683","S00020917":"8642758","S00020918":"6586761","S00020919":"6147650","S00020920":"9970224","S00020921":"6042404","S00020922":"7047741"},{"Metabolite":"DL-ASPARTIC ACID","S00020913":"4864664","S00020914":"4889908","S00020915":"3883201","S00020916":"4000330","S00020917":"2651796","S00020918":"2822236","S00020919":"2557041","S00020920":"3986783","S00020921":"3052645","S00020922":"2907860"},{"Metabolite":"OLEIC ACID","S00020913":"4231990","S00020914":"4697056","S00020915":"7536088","S00020916":"7587419","S00020917":"6995680","S00020918":"6676446","S00020919":"4950031","S00020920":"10692288","S00020921":"11600169","S00020922":"11426963"},{"Metabolite":"STEARIC ACID","S00020913":"20016122","S00020914":"15371109","S00020915":"17579309","S00020916":"17040678","S00020917":"13046999","S00020918":"20230254","S00020919":"15859216","S00020920":"18243587","S00020921":"15276115","S00020922":"17189468"},{"Metabolite":"ADENOSINE TRIPHOSPHATE","S00020913":"2660912","S00020914":"3309513","S00020915":"4112698","S00020916":"385391","S00020917":"2342068","S00020918":"265312","S00020919":"443067","S00020920":"2149398","S00020921":"82945","S00020922":"555266"},{"Metabolite":"L-GLUTAMINE","S00020913":"885575","S00020914":"811857","S00020915":"3081781","S00020916":"2640518","S00020917":"2715004","S00020918":"568768","S00020919":"548955","S00020920":"2923350","S00020921":"2303232","S00020922":"2323019"},{"Metabolite":"L-LYSINE","S00020913":"49088","S00020914":"41239","S00020915":"285413","S00020916":"276431","S00020917":"296903","S00020918":"52314","S00020919":"39772","S00020920":"348265","S00020921":"293363","S00020922":"330699"},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","S00020913":"4633774","S00020914":"4214840","S00020915":"3280832","S00020916":"2147441","S00020917":"3179734","S00020918":"1866116","S00020919":"2738734","S00020920":"4727528","S00020921":"1195479","S00020922":"3627633"},{"Metabolite":"DL-GLUTAMIC ACID","S00020913":"6941086","S00020914":"7913602","S00020915":"12005280","S00020916":"13170253","S00020917":"9894104","S00020918":"7591595","S00020919":"7223025","S00020920":"15540686","S00020921":"11240652","S00020922":"10767160"},{"Metabolite":"LACTIC ACID","S00020913":"3994","S00020914":"36936","S00020915":"8542","S00020916":"47451","S00020917":"59229","S00020918":"62827","S00020919":"2071","S00020920":"93600","S00020921":"3358","S00020922":"52449"},{"Metabolite":"GLYCERALDEHYDE 3-PHOSPHATE","S00020913":"2429","S00020914":"5218","S00020915":"7013","S00020916":"11021","S00020917":"17392","S00020918":"3240","S00020919":"2241","S00020920":"15329","S00020921":"8232","S00020922":"13605"},{"Metabolite":"HYPOXANTHINE","S00020913":"536277","S00020914":"656057","S00020915":"625438","S00020916":"603619","S00020917":"467856","S00020918":"440921","S00020919":"345946","S00020920":"662032","S00020921":"476493","S00020922":"439799"},{"Metabolite":"ACETOACETYL-COA","S00020913":"40934","S00020914":"38569","S00020915":"14512","S00020916":"11088","S00020917":"19702","S00020918":"20261","S00020919":"19872","S00020920":"19302","S00020921":"7097","S00020922":"7194"}],

"Metabolites":[{"Metabolite":"XANTHINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"1188","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"HEXOPYRANOSE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"206","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"CITRIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"311","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"DL-ASPARTIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"424","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"OLEIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"445639","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"STEARIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5281","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE TRIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5957","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-GLUTAMINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5961","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"L-LYSINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5962","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6022","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"DL-GLUTAMIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"611","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"LACTIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"612","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"GLYCERALDEHYDE 3-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"729","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"HYPOXANTHINE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"790","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ACETOACETYL-COA","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"92153","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}]
}

}