{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000737","ANALYSIS_ID":"AN001151","VERSION":"1","CREATED_ON":"July 3, 2017, 1:12 pm"},

"PROJECT":{"PROJECT_TITLE":"Metabolic change in macrophages after efferocytosis","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"To study metabolic change in macrophages after efferocytosis","INSTITUTE":"Northwestern University","DEPARTMENT":"Pathology","LABORATORY":"Thorp Lab","LAST_NAME":"Thorp","FIRST_NAME":"Edward","ADDRESS":"Evanston, IL","EMAIL":"ebthorp@northwestern.edu","PHONE":"312-503-4309"},

"STUDY":{"STUDY_TITLE":"Metabolic change in macrophages after efferocytosis (part II)","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"The goal of the experiment is to study metabolic pathway alteration in macrophages after efferocytosis (when macrophages eating apoptotic cells). Peritoneal macrophages were purified and plated in the cell-culture dishes. After culturing overnight. Apoptotic cells were overlayed to macrophages for 2 hours, then un-engulfed cells were removed through washing. Macrophages were left in the plate for another 4 hours before being lifted from the plate.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"4","TOTAL_SUBJECTS":"1"},

"SUBJECT":{"SUBJECT_TYPE":"Mouse","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0015592",
"Sample ID":"S00024013",
"Factors":{"Treatment":"none"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024014",
"Factors":{"Treatment":"none"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024015",
"Factors":{"Treatment":"apoptotic cells for 2hrs"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024016",
"Factors":{"Treatment":"apoptotic cells for 2hrs"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024017",
"Factors":{"Treatment":"apoptotic cells for 2hrs"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024018",
"Factors":{"Treatment":"ko untreated"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024019",
"Factors":{"Treatment":"ko untreated"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024020",
"Factors":{"Treatment":"ko treated with apoptotic cells 2hrs"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024021",
"Factors":{"Treatment":"ko treated with apoptotic cells 2hrs"}
},
{
"Subject ID":"SU0015592",
"Sample ID":"S00024022",
"Factors":{"Treatment":"ko treated with apoptotic cells 2hrs"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A011 - Glycolysis-TCA-nucleotides.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890N","COLUMN_NAME":"Agilent HP5-MS (15m × 0.25mm, 0.25 um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A011 - Glycolysis-TCA-nucleotides.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"pmol/ug protein",

"Data":[{"Metabolite":"D-ERYTHROSE 4-PHOSPHATE","S00024013":".00106586","S00024014":"0","S00024015":".001958876","S00024016":".001292215","S00024017":"0","S00024018":".007599994","S00024019":".008146643","S00024020":".003979795","S00024021":".005497746","S00024022":".003390573"},{"Metabolite":"D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE)","S00024013":".003890481","S00024014":".001945037","S00024015":".005114715","S00024016":".002222935","S00024017":".004980454","S00024018":".008437272","S00024019":".006940036","S00024020":".007476818","S00024021":".006812015","S00024022":".008952233"},{"Metabolite":"CITRIC ACID","S00024013":".020915117","S00024014":".017025853","S00024015":".021440712","S00024016":".028471986","S00024017":".017258225","S00024018":".065730765","S00024019":".069203622","S00024020":".071994548","S00024021":".054862786","S00024022":".037426972"},{"Metabolite":"FAD","S00024013":"0","S00024014":"0","S00024015":"0","S00024016":"0","S00024017":"0","S00024018":"0","S00024019":"0","S00024020":".0000348907","S00024021":".000044205","S00024022":"0"},{"Metabolite":"MALIC ACID","S00024013":".012652249","S00024014":".011595965","S00024015":".024237432","S00024016":".034194884","S00024017":".021873915","S00024018":".032693286","S00024019":".044060541","S00024020":".039575982","S00024021":".037726584","S00024022":".031340399"},{"Metabolite":"ADENOSINE TRIPHOSPHATE","S00024013":".002151351","S00024014":".00204589","S00024015":".002234721","S00024016":".002060296","S00024017":".002174957","S00024018":".003671275","S00024019":".003597657","S00024020":".002302726","S00024021":".001890725","S00024022":".002201398"},{"Metabolite":"ROBISON ESTER","S00024013":".005113045","S00024014":".003987889","S00024015":".00426487","S00024016":".003153601","S00024017":".002516779","S00024018":".006615638","S00024019":".020124577","S00024020":".008297248","S00024021":".008095753","S00024022":".00623114"},{"Metabolite":"SUCROSE","S00024013":"0","S00024014":"0","S00024015":"0","S00024016":"0","S00024017":"0","S00024018":".001716088","S00024019":".002007621","S00024020":".012244551","S00024021":".014661049","S00024022":".008125526"},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","S00024013":".002222532","S00024014":".00201846","S00024015":".002886605","S00024016":".002735473","S00024017":".003192554","S00024018":".005605718","S00024019":".00544571","S00024020":".005237412","S00024021":".003537134","S00024022":".004722127"},{"Metabolite":"ADENOSINE 5'-MONOPHOSPHATE","S00024013":".006053719","S00024014":".003565046","S00024015":".003834798","S00024016":".003334564","S00024017":".006877784","S00024018":".013749327","S00024019":".014710731","S00024020":".013603214","S00024021":".004438048","S00024022":".012762911"},{"Metabolite":"GLYCERATE 3-PHOSPHATE","S00024013":"0","S00024014":"0","S00024015":"0","S00024016":"0","S00024017":"0","S00024018":".000232519","S00024019":"0","S00024020":"0","S00024021":"0","S00024022":"0"},{"Metabolite":"RIBOSE PHOSPHATE","S00024013":".006054687","S00024014":".007080436","S00024015":".01366128","S00024016":".015745723","S00024017":".007121562","S00024018":".012813887","S00024019":".014748332","S00024020":".013491506","S00024021":".021274454","S00024022":".010218364"},{"Metabolite":"6-PHOSPHOGLUCONIC ACID","S00024013":".001497523","S00024014":".001529843","S00024015":".001540677","S00024016":".001544017","S00024017":".001526052","S00024018":".002816493","S00024019":".002646342","S00024020":".001274555","S00024021":".001234628","S00024022":".001294086"}],

"Metabolites":[{"Metabolite":"D-ERYTHROSE 4-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"122357","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"21125049","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"CITRIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"311","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"FAD","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"46906035","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"MALIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"525","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE TRIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5957","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ROBISON ESTER","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5958","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"SUCROSE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5988","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6022","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE 5'-MONOPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6083","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"GLYCERATE 3-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"724","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"RIBOSE PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"77982","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"6-PHOSPHOGLUCONIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"91493","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}]
}

}