{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000835","ANALYSIS_ID":"AN001336","VERSION":"1","CREATED_ON":"August 9, 2017, 9:44 am"},

"PROJECT":{"PROJECT_TITLE":"Influence of murine norovirus on cell metabolism","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"C6_effects_in_RAW","INSTITUTE":"University of Michigan","DEPARTMENT":"Microbiology & Immunology","LABORATORY":"Passalacqua Lab","LAST_NAME":"Passalacqua","FIRST_NAME":"Karla","ADDRESS":"Ann Arbor, MI","EMAIL":"kpassal@umich.edu","PHONE":"734-763-5639"},

"STUDY":{"STUDY_TITLE":"Deubiquitinase inhibitor compound C6 effect on cellular metabolism","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"6.0 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of fresh medium with either 2.5 micromolar Compound C6 or a v/v match of DMSO (vehicle control) was added to the cells. Cells were incubated at 37 degrees in 5% CO2 for 30 minutes. Cells were washed 3X with cold DPBS++ and 10 mL of medium was added to the cells with either 1.0 micromolar Compound C6 or v/v match of DMSO. Cells were incubated at 37 degrees and 5% CO2 for 7.0 hours. Cells were washed with 10 mL of 150 mM Ammonium Acetate, swirled, washed again with 5 mL, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"7","TOTAL_SUBJECTS":"1","STUDY_COMMENTS":"I request that the metabolites of the TCA+ assay that are to be assessed quantitatively please be sure to include ATP, ADP, AMP, NAD, NADH, NADP, NADPH, E4P, S7P, 6PG, G3P, H6P, and Lactate in particular. Also, I would request that both reduced and oxidized Glutathione as well as Fructose 1,6-bisphosphate be included in the non-quantitative data."},

"SUBJECT":{"SUBJECT_TYPE":"MOUSE","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0016447",
"Sample ID":"S00026310",
"Factors":{"Sample type":"Cell Line","Treatment":"Vehicle","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026311",
"Factors":{"Sample type":"Cell Line","Treatment":"Vehicle","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026312",
"Factors":{"Sample type":"Cell Line","Treatment":"Vehicle","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026313",
"Factors":{"Sample type":"Cell Line","Treatment":"Vehicle","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026314",
"Factors":{"Sample type":"Cell Line","Treatment":"Vehicle","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026315",
"Factors":{"Sample type":"Cell Line","Treatment":"DUB inhibitor","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026316",
"Factors":{"Sample type":"Cell Line","Treatment":"DUB inhibitor","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026317",
"Factors":{"Sample type":"Cell Line","Treatment":"DUB inhibitor","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026318",
"Factors":{"Sample type":"Cell Line","Treatment":"DUB inhibitor","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026319",
"Factors":{"Sample type":"Cell Line","Treatment":"DUB inhibitor","Type":"RAW"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026320",
"Factors":{"Sample type":"Macrophages","Treatment":"Control-Other_Cells","Type":"Primary BL6 BMM"}
},
{
"Subject ID":"SU0016447",
"Sample ID":"S00026321",
"Factors":{"Sample type":"Macrophages","Treatment":"Control-Other_Cells","Type":"Primary BL6 BMM"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A043-TCA-Plus-20170713.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A043-TCA-Plus-20170713.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6520B QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"ISTD response ratio",

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}

}