{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000839","ANALYSIS_ID":"AN001345","VERSION":"1","CREATED_ON":"August 9, 2017, 10:55 am"},

"PROJECT":{"PROJECT_TITLE":"Thermal Modalities Study","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"This study is looking at the effect of cooling on muscle tissue metabolism","INSTITUTE":"University of Michigan","DEPARTMENT":"Orthopaedic Surgery","LABORATORY":"Mendias Lab","LAST_NAME":"Mendias","FIRST_NAME":"Christopher","ADDRESS":"Ann Arbor, MI","EMAIL":"cmendias@umich.edu","PHONE":"734-764-3250"},

"STUDY":{"STUDY_TITLE":"Effect of cooling on muscle tissue metabolism (part II)","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"Thermal modalities are commonly used in sports medicine to affect tissue healing. Cold therapy is commonly used modalities, but the metabolic changes to muscle after cooling are not known. The objective of this study is to look at the effect of cooling on muscle metabolites and gene expression. There are a total of 8 subjects in the study. Each subject had an ice cup cryotherapy treatment (cool samples) to one leg for 15 minutes, and the other leg served as the control (cntrl samples). Two hours after the application of cryotherapy, a biopsy was taken from each thigh muscle. Muscle was minced with scissors and quickly snap frozen in liquid nitrogen.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"10","TOTAL_SUBJECTS":"8","STUDY_COMMENTS":"We would like to run the TCA Plus Analysis on each of the samples. For the statistical analysis, we would normalize the cool side to the ctrl side, and look at the relative change in metabolite abundance as a result of the cooling procedure. only used in sports medicine to affect tissue healing."},

"SUBJECT":{"SUBJECT_TYPE":"HUMAN","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0014908",
"Sample ID":"S00023215",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"1"}
},
{
"Subject ID":"SU0014908",
"Sample ID":"S00023216",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"1"}
},
{
"Subject ID":"SU0014909",
"Sample ID":"S00023217",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"2"}
},
{
"Subject ID":"SU0014909",
"Sample ID":"S00023218",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"2"}
},
{
"Subject ID":"SU0014910",
"Sample ID":"S00023219",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"3"}
},
{
"Subject ID":"SU0014910",
"Sample ID":"S00023220",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"3"}
},
{
"Subject ID":"SU0014911",
"Sample ID":"S00023221",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"4"}
},
{
"Subject ID":"SU0014911",
"Sample ID":"S00023222",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"4"}
},
{
"Subject ID":"SU0014912",
"Sample ID":"S00023223",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"5"}
},
{
"Subject ID":"SU0014912",
"Sample ID":"S00023224",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"5"}
},
{
"Subject ID":"SU0014913",
"Sample ID":"S00023225",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"6"}
},
{
"Subject ID":"SU0014913",
"Sample ID":"S00023226",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"6"}
},
{
"Subject ID":"SU0014914",
"Sample ID":"S00023227",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"7"}
},
{
"Subject ID":"SU0014914",
"Sample ID":"S00023228",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"7"}
},
{
"Subject ID":"SU0014915",
"Sample ID":"S00023229",
"Factors":{"Temperature":"Control"},
"Additional sample data":{"Subject":"8"}
},
{
"Subject ID":"SU0014915",
"Sample ID":"S00023230",
"Factors":{"Temperature":"Cooled"},
"Additional sample data":{"Subject":"8"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A011_Glycolysis-TCA-nucleotides.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A011 - Glycolysis-TCA-nucleotides.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6520B QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"nmol/mg",

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}

}