{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000931","ANALYSIS_ID":"AN001525","VERSION":"1","CREATED_ON":"February 28, 2018, 3:40 pm"},

"PROJECT":{"PROJECT_TITLE":"FLUTE: Functionalizing Lists of Unknown TB Entities","PROJECT_TYPE":"NIH U19","PROJECT_SUMMARY":"Functional studies of proteins of unknown function in Mycobacterium tuberculosis","INSTITUTE":"Texas A&M University","LAST_NAME":"Ioerger","FIRST_NAME":"Thomas","ADDRESS":"310 Bright Bldg, TAMUS 3112, College Station, TX 77843","EMAIL":"ioerger@cs.tamu.edu","PHONE":"9792291494","FUNDING_SOURCE":"NIH U19 AI107774"},

"STUDY":{"STUDY_TITLE":"Activity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv3915 (CwlM)","STUDY_TYPE":"timecourse","STUDY_SUMMARY":"These experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.","INSTITUTE":"Weill Cornell Medical College, NY","LAST_NAME":"Rhee","FIRST_NAME":"Kyu","ADDRESS":"Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY","EMAIL":"kyr9001@med.cornell.edu","PHONE":"979-229-1494"},

"SUBJECT":{"SUBJECT_TYPE":"Bacteria","SUBJECT_SPECIES":"Mycobacterium tuberculosis H37Rv","TAXONOMY_ID":"Mycobacterium tuberculosis H37Rv, taxid: 83332"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_A_0",
"Factors":{"timepoint (min)":"-","protein prep":"Active"},
"Additional sample data":{"replicate":"1"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_B_0",
"Factors":{"timepoint (min)":"-","protein prep":"Active"},
"Additional sample data":{"replicate":"2"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_C_0",
"Factors":{"timepoint (min)":"-","protein prep":"Active"},
"Additional sample data":{"replicate":"3"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_A_15",
"Factors":{"timepoint (min)":"15","protein prep":"Active"},
"Additional sample data":{"replicate":"1"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_B_15",
"Factors":{"timepoint (min)":"15","protein prep":"Active"},
"Additional sample data":{"replicate":"2"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_C_15",
"Factors":{"timepoint (min)":"15","protein prep":"Active"},
"Additional sample data":{"replicate":"3"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_A_30",
"Factors":{"timepoint (min)":"30","protein prep":"Active"},
"Additional sample data":{"replicate":"1"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_B_30",
"Factors":{"timepoint (min)":"30","protein prep":"Active"},
"Additional sample data":{"replicate":"2"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"CwlM_C_30",
"Factors":{"timepoint (min)":"30","protein prep":"Active"},
"Additional sample data":{"replicate":"3"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_A_0",
"Factors":{"timepoint (min)":"-","protein prep":"Inactive"},
"Additional sample data":{"replicate":"1"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_B_0",
"Factors":{"timepoint (min)":"-","protein prep":"Inactive"},
"Additional sample data":{"replicate":"2"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_C_0",
"Factors":{"timepoint (min)":"-","protein prep":"Inactive"},
"Additional sample data":{"replicate":"3"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_A_15",
"Factors":{"timepoint (min)":"15","protein prep":"Inactive"},
"Additional sample data":{"replicate":"1"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_B_15",
"Factors":{"timepoint (min)":"15","protein prep":"Inactive"},
"Additional sample data":{"replicate":"2"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_C_15",
"Factors":{"timepoint (min)":"15","protein prep":"Inactive"},
"Additional sample data":{"replicate":"3"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_A_30",
"Factors":{"timepoint (min)":"30","protein prep":"Inactive"},
"Additional sample data":{"replicate":"1"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_B_30",
"Factors":{"timepoint (min)":"30","protein prep":"Inactive"},
"Additional sample data":{"replicate":"2"}
},
{
"Subject ID":"Rv3915",
"Sample ID":"Inactivated-CwlM_C_30",
"Factors":{"timepoint (min)":"30","protein prep":"Inactive"},
"Additional sample data":{"replicate":"3"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Rv3915 from M. tuberculosis H37Rv was expressed in E. coli cells and purified.","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated)."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Aqueous normal phase diamond hydride","CHROMATOGRAPHY_TYPE":"Normal phase","INSTRUMENT_NAME":"Agilent 1290","COLUMN_NAME":"Microsolv Diamond hydride (150mm, 4um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6220 TOF","INSTRUMENT_TYPE":"TOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_RESULTS_FILE":"ST000931_AN001526_Results.txt UNITS:integrated peak counts"}

}