{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001297","ANALYSIS_ID":"AN002158","VERSION":"1","CREATED_ON":"January 5, 2020, 6:08 pm"},

"PROJECT":{"PROJECT_TITLE":"High Cellulose diet impact on gut inflammation","PROJECT_SUMMARY":"Dietary cellulose prevents gut inflammation by modulating lipid metabolism and gut microbiota","INSTITUTE":"Asan Medical Center","LAST_NAME":"Yoo","FIRST_NAME":"Hyun Ju","ADDRESS":"88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea","EMAIL":"yoohyunju@amc.seoul.kr","PHONE":"82-02-3010-4029"},

"STUDY":{"STUDY_TITLE":"High Cellulose diet impact on gut inflammation","STUDY_SUMMARY":"Dietary cellulose prevents gut inflammation by modulating lipid metabolism and gut microbiota","INSTITUTE":"Asan Medical Center","LAST_NAME":"Yoo","FIRST_NAME":"Hyun Ju","ADDRESS":"88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea","EMAIL":"yoohyunju@amc.seoul.kr","PHONE":"82-02-3010-4029"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"High_1",
"Factors":{"Cellulose(%)":"30"},
"Additional sample data":{"RAW_FILE_NAME":"High_1.zip"}
},
{
"Subject ID":"-",
"Sample ID":"High_2",
"Factors":{"Cellulose(%)":"30"},
"Additional sample data":{"RAW_FILE_NAME":"High_2.zip"}
},
{
"Subject ID":"-",
"Sample ID":"High_3",
"Factors":{"Cellulose(%)":"30"},
"Additional sample data":{"RAW_FILE_NAME":"High_3.zip"}
},
{
"Subject ID":"-",
"Sample ID":"High_4",
"Factors":{"Cellulose(%)":"30"},
"Additional sample data":{"RAW_FILE_NAME":"High_4.zip"}
},
{
"Subject ID":"-",
"Sample ID":"High_5",
"Factors":{"Cellulose(%)":"30"},
"Additional sample data":{"RAW_FILE_NAME":"High_5.zip"}
},
{
"Subject ID":"-",
"Sample ID":"Low_1",
"Factors":{"Cellulose(%)":"0.3"},
"Additional sample data":{"RAW_FILE_NAME":"Low_1.zip"}
},
{
"Subject ID":"-",
"Sample ID":"Low_2",
"Factors":{"Cellulose(%)":"0.3"},
"Additional sample data":{"RAW_FILE_NAME":"Low_2.zip"}
},
{
"Subject ID":"-",
"Sample ID":"Low_3",
"Factors":{"Cellulose(%)":"0.3"},
"Additional sample data":{"RAW_FILE_NAME":"Low_3.zip"}
},
{
"Subject ID":"-",
"Sample ID":"Low_4",
"Factors":{"Cellulose(%)":"0.3"},
"Additional sample data":{"RAW_FILE_NAME":"Low_4.zip"}
},
{
"Subject ID":"-",
"Sample ID":"Low_5",
"Factors":{"Cellulose(%)":"0.3"},
"Additional sample data":{"RAW_FILE_NAME":"Low_5.zip"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cecum contents were collected and freeze-dried for further analysis.","SAMPLE_TYPE":"Cecum"},

"TREATMENT":{"TREATMENT_SUMMARY":"Freeze-dried cecum contents were stored at liquid N2 until metabolomics study."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Metabolites were extracted from cecum contents using conventional liquid-liquid extraction method. (Folch method) Internal standard were added to the samples before extraction. Nonpolar and polar metabolites were obtained from organic and aqueous phases, respectively."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"C18_pos","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Pursuit C18 (2.1 x 150 mm)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Xcaliber","MS_RESULTS_FILE":"ST001297_AN002158_Results.txt UNITS:cps Has m/z:Yes Has RT:Yes RT units:Minutes"}

}