{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001306","ANALYSIS_ID":"AN002175","VERSION":"1","CREATED_ON":"January 24, 2020, 11:21 am"},

"PROJECT":{"PROJECT_TITLE":"Biomolecular analyses of hypospadias according to severity","PROJECT_SUMMARY":"Hypospadias, characterized by the displacement of the opening of the urethra at any point in the medial-ventral side of the penis, is classified upon severity as mild (Type I) and severe (Type II and Type III) hypospadias. Hypospadias’ etiology is idiopathic in the majority of cases, and underlying causes seem of multifactorial origin. Studies regarding genetic variants support this notion. It is unknown whether downstream gene products fit this profile. This study evaluated the metabolome of hypospadias by using the emerging technology of metabolomics in the search for distinct cellular processes associated with hypospadias’ etiology according to the severity of this congenital urogenital condition. Foreskin samples were collected during urethroplasty from boys with Type I, II, and III hypospadias or undergoing elective circumcision (N=28) between 5 to 28 months of age. Samples were processed and submitted to gas chromatography-mass spectrometry (GC/MS). MetaboloAnalyst (http://www.metaboanalyst.ca/) online platform was used for bioinformatic analyses. The metabolome of Type II and Type III hypospadias patients differs from the metabolome of Type I hypospadias and control patients. Thirty-five metabolites were identified by GC/MS. Of those, 14 metabolites, amino acids, were found in significantly low concentrations in Type II and Type III hypospadias in comparison to Type I hypospadias and controls. Amino acids comprised asparagine, aspartate, glutamate, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, and tyrosine. The difference observed in the metabolome between severe and mild hypospadias supports previous research work of plausible severity-dependent etiologies for hypospadias. The observed downregulation of specific amino acids in severe hypospadias provides alternative routes for future research aiming to identify disrupted networks and pathways while considering the severity of hypospadias.","INSTITUTE":"University of Puerto Rico, Medical Sciences Campus","DEPARTMENT":"Anatomy & Neurobiology","LAST_NAME":"Piñeyro-Ruiz","FIRST_NAME":"Coriness","ADDRESS":"University of Puerto Rico, Medical Sciences Campus, Department of Anatomy & Neurobiology, Main Building, 5th Floor, Room A-521 PO BOX 365067 San Juan, PR 00936-5067","EMAIL":"coriness.pineyro@upr.edu","PHONE":"7877582525 ext. 1506","FUNDING_SOURCE":"MBRS-RISE (R25GM061838); CCRHD-NIMHD RCMI (U54M007600); NIGMS-INBRE-PR NIH (5P20GM103475-16); NIMHD & NIAID (2U54MD007587)"},

"STUDY":{"STUDY_TITLE":"Biomolecular analyses of hypospadias according to severity","STUDY_SUMMARY":"Hypospadias, characterized by the displacement of the opening of the urethra at any point in the medial-ventral side of the penis, is classified upon severity as mild (Type I) and severe (Type II and Type III) hypospadias. Hypospadias’ etiology is idiopathic in the majority of cases, and underlying causes seem of multifactorial origin. Studies regarding genetic variants support this notion. It is unknown whether downstream gene products fit this profile. This study evaluated the metabolome of hypospadias by using the emerging technology of metabolomics in the search for distinct cellular processes associated with hypospadias’ etiology according to the severity of this congenital urogenital condition. Foreskin samples were collected during urethroplasty from boys with Type I, II, and III hypospadias or undergoing elective circumcision (N=28) between 5 to 28 months of age. Samples were processed and submitted to gas chromatography-mass spectrometry (GC/MS). MetaboloAnalyst (http://www.metaboanalyst.ca/) online platform was used for bioinformatic analyses. The metabolome of Type II and Type III hypospadias patients differs from the metabolome of Type I hypospadias and control patients. Thirty-five metabolites were identified by GC/MS. Of those, 14 metabolites, amino acids, were found in significantly low concentrations in Type II and Type III hypospadias in comparison to Type I hypospadias and controls. Amino acids comprised asparagine, aspartate, glutamate, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, and tyrosine. The difference observed in the metabolome between severe and mild hypospadias supports previous research work of plausible severity-dependent etiologies for hypospadias. The observed downregulation of specific amino acids in severe hypospadias provides alternative routes for future research aiming to identify disrupted networks and pathways while considering the severity of hypospadias.","INSTITUTE":"University of Puerto Rico, Medical Sciences Campus","DEPARTMENT":"Anatomy & Neurobiology","LAST_NAME":"Piñeyro-Ruiz","FIRST_NAME":"Coriness","ADDRESS":"University of Puerto Rico, Medical Sciences Campus, Department of Anatomy & Neurobiology, Main Building, 5th Floor, Room A-521 PO BOX 365067 San Juan, PR 00936-5067","EMAIL":"coriness.pineyro@upr.edu","PHONE":"7877582525","NUM_GROUPS":"4","TOTAL_SUBJECTS":"28","NUM_MALES":"28"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"5-28 months","GENDER":"Male","HUMAN_ETHNICITY":"Hispanic","HUMAN_INCLUSION_CRITERIA":"(1) boys diagnosed with hypospadias with no comorbidities; (2) children undergoing elective circumcision without a clinical diagnosis; (3) age must be between 0 to 28 months; (4) parents' authorization and sign inform consent","HUMAN_EXCLUSION_CRITERIA":"(1) being unable to understand spoken and written language in Spanish; (2) not being willing to provide authorization for collection of tissue sample during the surgical repair of hypospadias and circumcision procedure"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"H8-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"13","RAW_FILE_NAME":"H-008_UNK-0003_4302019_3"}
},
{
"Subject ID":"-",
"Sample ID":"H15-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"28","RAW_FILE_NAME":"H-015_UNK-0006_4302019_6"}
},
{
"Subject ID":"-",
"Sample ID":"H21-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"24","RAW_FILE_NAME":"H-021_UNK-0009_4302019_9"}
},
{
"Subject ID":"-",
"Sample ID":"H22-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"28","RAW_FILE_NAME":"H-022_UNK-0010_4302019_10"}
},
{
"Subject ID":"-",
"Sample ID":"H27-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"13","RAW_FILE_NAME":"H-027_UNK-0014_4302019_14"}
},
{
"Subject ID":"-",
"Sample ID":"H28-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"7","RAW_FILE_NAME":"H-028_UNK-0015_4302019_15"}
},
{
"Subject ID":"-",
"Sample ID":"H29-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"7","RAW_FILE_NAME":"H-029_UNK-0016_4302019_16"}
},
{
"Subject ID":"-",
"Sample ID":"H42-C",
"Factors":{"Condition":"Control"},
"Additional sample data":{"Age":"28","RAW_FILE_NAME":"H-042_UNK-0028_4302019_28"}
},
{
"Subject ID":"-",
"Sample ID":"H3-I",
"Factors":{"Condition":"Type I"},
"Additional sample data":{"Age":"22","RAW_FILE_NAME":"H-003_UNK-0002_4302019_2"}
},
{
"Subject ID":"-",
"Sample ID":"H12-I",
"Factors":{"Condition":"Type I"},
"Additional sample data":{"Age":"6","RAW_FILE_NAME":"H-012_UNK-0004_4302019_4"}
},
{
"Subject ID":"-",
"Sample ID":"H20-I",
"Factors":{"Condition":"Type I"},
"Additional sample data":{"Age":"6","RAW_FILE_NAME":"H-020_UNK-0008_4302019_8"}
},
{
"Subject ID":"-",
"Sample ID":"H23-I",
"Factors":{"Condition":"Type I"},
"Additional sample data":{"Age":"6","RAW_FILE_NAME":"H-023_UNK-0011_4302019_11"}
},
{
"Subject ID":"-",
"Sample ID":"H26-I",
"Factors":{"Condition":"Type I"},
"Additional sample data":{"Age":"5","RAW_FILE_NAME":"H-026_UNK-0013_4302019_13"}
},
{
"Subject ID":"-",
"Sample ID":"H32-I",
"Factors":{"Condition":"Type I"},
"Additional sample data":{"Age":"23","RAW_FILE_NAME":"H-032_UNK-0019_4302019_19"}
},
{
"Subject ID":"-",
"Sample ID":"H40-I",
"Factors":{"Condition":"Type I"},
"Additional sample data":{"Age":"14","RAW_FILE_NAME":"H-040_UNK-0026_4302019_26"}
},
{
"Subject ID":"-",
"Sample ID":"H24-II",
"Factors":{"Condition":"Type II"},
"Additional sample data":{"Age":"6","RAW_FILE_NAME":"H-024_UNK-0012_4302019_12"}
},
{
"Subject ID":"-",
"Sample ID":"H30-II",
"Factors":{"Condition":"Type II"},
"Additional sample data":{"Age":"6","RAW_FILE_NAME":"H-030_UNK-0017_4302019_17"}
},
{
"Subject ID":"-",
"Sample ID":"H31-II",
"Factors":{"Condition":"Type II"},
"Additional sample data":{"Age":"5","RAW_FILE_NAME":"H-031_UNK-0018_4302019_18"}
},
{
"Subject ID":"-",
"Sample ID":"H33-II",
"Factors":{"Condition":"Type II"},
"Additional sample data":{"Age":"6","RAW_FILE_NAME":"H-033_UNK-0020_4302019_20"}
},
{
"Subject ID":"-",
"Sample ID":"H35-II",
"Factors":{"Condition":"Type II"},
"Additional sample data":{"Age":"10","RAW_FILE_NAME":"H-035_UNK-0021_4302019_21"}
},
{
"Subject ID":"-",
"Sample ID":"H36-II",
"Factors":{"Condition":"Type II"},
"Additional sample data":{"Age":"9","RAW_FILE_NAME":"H-036_UNK-0022_4302019_22"}
},
{
"Subject ID":"-",
"Sample ID":"H43-II",
"Factors":{"Condition":"Type II"},
"Additional sample data":{"Age":"5","RAW_FILE_NAME":"H-043_UNK-0029_4302019_29"}
},
{
"Subject ID":"-",
"Sample ID":"H14-III",
"Factors":{"Condition":"Type III"},
"Additional sample data":{"Age":"7","RAW_FILE_NAME":"H-014_UNK-0005_4302019_5"}
},
{
"Subject ID":"-",
"Sample ID":"H37-III",
"Factors":{"Condition":"Type III"},
"Additional sample data":{"Age":"5","RAW_FILE_NAME":"H-037_UNK-0023_4302019_23"}
},
{
"Subject ID":"-",
"Sample ID":"H38-III",
"Factors":{"Condition":"Type III"},
"Additional sample data":{"Age":"5","RAW_FILE_NAME":"H-038_UNK-0024_4302019_24"}
},
{
"Subject ID":"-",
"Sample ID":"H39-III",
"Factors":{"Condition":"Type III"},
"Additional sample data":{"Age":"6","RAW_FILE_NAME":"H-039_UNK-0025_4302019_25"}
},
{
"Subject ID":"-",
"Sample ID":"H41-III",
"Factors":{"Condition":"Type III"},
"Additional sample data":{"Age":"7","RAW_FILE_NAME":"H-041_UNK-0027_4302019_27"}
},
{
"Subject ID":"-",
"Sample ID":"H44-III",
"Factors":{"Condition":"Type III"},
"Additional sample data":{"Age":"5","RAW_FILE_NAME":"H-044_UNK-0030_4302019_30"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Foreskin samples were collected from children undergoing urethroplasty and boys scheduled for elective circumcision at the University District Hospital, San Juan, Puerto Rico, and HIMA San Pablo Hospital, Caguas, Puerto Rico. Samples were immediately frozen in dry ice and stored at -80 °C.","SAMPLE_TYPE":"Foreskin"},

"TREATMENT":{"TREATMENT_SUMMARY":"No treatment."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Foreskin samples are cut into small pieces with a scalpel (new) on ice. Weight 50 mg of the foreskin for each sample in 1.5 mL Eppendorf tubes. Sample homogenization and extraction of metabolites is perform adding 1 ml of chloroform/MeOH/H2O (2:5:2) to each sample. Samples are sonicated using a polytron for 15 seconds (3X) in a beaker with ice. Shake samples for 15 minutes in a nutating vortex and centrifuge at 13000 rpm for 10 minutes at 4°C. The supernatant is collected and transferred into 1.5 glass vials for drying. For evaporation, use plastic caps with hole caps. Make a little hole in the center for evaporation to take place. Evaporate samples in SpeedVac until samples are completely dried."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Shimadzu GCMS-QP2010 ultra","COLUMN_NAME":"Shimadzu SH-Rxi-5ms (30 x 0.25 x 0.25)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"PRINBRE Metabolomics Research Core"},

"MS":{"INSTRUMENT_NAME":"Shimadzu QP2010 Ultra","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"Unspecified","MS_COMMENTS":"Data was processed using GCMS Labsolution data analysis software (Shimadzu) equipped with NIST14/2014/EPA/NIH database."},

"MS_METABOLITE_DATA":{
"Units":"millimolar",

"Data":[{"Metabolite":"Cholesta-3,5-diene","H8-C":"0.027851466","H15-C":"0.008045074","H21-C":"0.018329704","H22-C":"0.010423374","H27-C":"0.036694313","H28-C":"0.065092765","H29-C":"0.046986126","H42-C":"0.014450425","H3-I":"0.071095752","H12-I":"0.033363514","H20-I":"0.041190376","H23-I":"0.057586602","H26-I":"0.054415544","H32-I":"0.046380142","H40-I":"0.01689086","H24-II":"0.036616987","H30-II":"0.03860037","H31-II":"0.021985925","H33-II":"0.040429657","H35-II":"","H36-II":"0.012109304","H43-II":"0.0148056","H14-III":"0.022398031","H37-III":"0.029403924","H38-III":"0.019901254","H39-III":"0.025998823","H41-III":"0.027858661","H44-III":"0.020937123"},{"Metabolite":"Cholesterol","H8-C":"5.979689167","H15-C":"1.69614905","H21-C":"3.10635413","H22-C":"5.860631966","H27-C":"5.438127339","H28-C":"5.188912319","H29-C":"6.340454997","H42-C":"4.489258483","H3-I":"4.230567633","H12-I":"5.892264115","H20-I":"4.719577343","H23-I":"6.753515223","H26-I":"4.447240286","H32-I":"6.767846193","H40-I":"5.710485091","H24-II":"5.324835536","H30-II":"7.119982399","H31-II":"4.546098644","H33-II":"6.215396535","H35-II":"5.548858512","H36-II":"4.998756382","H43-II":"4.122927197","H14-III":"5.419725891","H37-III":"6.084313584","H38-III":"5.440926601","H39-III":"4.715725424","H41-III":"8.206192758","H44-III":"5.092611433"},{"Metabolite":"Aspartic 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"Metabolites":[{"Metabolite":"Cholesta-3,5-diene","retention index":"2899","KEGG ID":"C05439"},{"Metabolite":"Cholesterol","retention index":"3506","KEGG ID":"C00187"},{"Metabolite":"Aspartic acid","retention index":"2155","KEGG ID":"C00049"},{"Metabolite":"Alanine","retention index":"1537","KEGG ID":"C00041"},{"Metabolite":"Glycine","retention index":"1559","KEGG ID":"C00037"},{"Metabolite":"beta-Alanine","retention index":"1640","KEGG ID":"C00099"},{"Metabolite":"Valine","retention index":"1658","KEGG ID":"C00183"},{"Metabolite":"Leucine","retention index":"1698","KEGG ID":"C00123"},{"Metabolite":"Isoleucine","retention index":"1731","KEGG ID":"C00407"},{"Metabolite":"Proline","retention index":"1769","KEGG ID":"C00148"},{"Metabolite":"Methionine","retention index":"1970","KEGG ID":"C00073"},{"Metabolite":"Serine","retention index":"1992","KEGG ID":"C00065"},{"Metabolite":"Threonine","retention index":"2024","KEGG ID":"C00188"},{"Metabolite":"Phenylalanine","retention index":"2093","KEGG ID":"C00079"},{"Metabolite":"Glutamic acid","retention index":"2272","KEGG ID":"C00025"},{"Metabolite":"Asparagine","retention index":"2305","KEGG ID":"C00152"},{"Metabolite":"Lysine","retention index":"2380","KEGG ID":"C00047"},{"Metabolite":"Glutamine","retention index":"2425","KEGG ID":"C00064"},{"Metabolite":"Histidine","retention index":"2594","KEGG ID":"C00135"},{"Metabolite":"Tyrosine","retention index":"2643","KEGG ID":"C00082"},{"Metabolite":"Tryptophan","retention index":"2690","KEGG ID":"C00078"},{"Metabolite":"Palmitic acid","retention index":"2282","KEGG ID":"C00249"},{"Metabolite":"Oleic acid","retention index":"2460","KEGG ID":"C00712"},{"Metabolite":"Stearic acid","retention index":"2483","KEGG ID":"C01530"},{"Metabolite":"Behenic acid","retention index":"2885","KEGG ID":"C08281"},{"Metabolite":"Methylmalonic acid","retention index":"1758","KEGG ID":"C02170"},{"Metabolite":"Citric acid","retention index":"2621","KEGG ID":"C00158"},{"Metabolite":"Lactic acid","retention index":"1491","KEGG ID":"C00186"},{"Metabolite":"3-Hydroxybutyric acid","retention index":"1592","KEGG ID":"C01089"},{"Metabolite":"2-Hydroxybutyric acid","retention index":"1596","KEGG ID":"C05984"},{"Metabolite":"Malic acid","retention index":"2111","KEGG ID":"C00149"},{"Metabolite":"Urea","retention index":"1652","KEGG ID":"C00086"},{"Metabolite":"Histamine","retention index":"2082","KEGG ID":"C00388"},{"Metabolite":"Mandelic acid","retention index":"2798","KEGG ID":"C01983"},{"Metabolite":"Uric acid","retention index":"2878","KEGG ID":"C00366"}]
}

}