{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001376","ANALYSIS_ID":"AN002296","VERSION":"1","CREATED_ON":"02-08-2024"}, "PROJECT":{"PROJECT_TITLE":"TCDD-elicited effects on metabolite levels in liver of male mice treated with sesame oil","PROJECT_TYPE":"Untargetd Metabolomics","PROJECT_SUMMARY":"Experiment to study TCDD-elicited effects on metabolite levels in liver of male mice treated with sesame oil vehicle or 0.01-30 ug/kg TCDD every 4 days for 28 days.","INSTITUTE":"Michigan State University","DEPARTMENT":"Biochemistry and Molecular Biology","LAST_NAME":"Fling","FIRST_NAME":"Russell","ADDRESS":"1129 Farm Ln, East Lansing, MI,USA","EMAIL":"flingrus@msu.edu","PHONE":"(517) 884-2054","PUBLICATIONS":"-","DOI":"http://dx.doi.org/10.21228/M8912S"}, "STUDY":{"STUDY_TITLE":"2,3,7,8 -Tetrachlorodibenzo-p-dioxin Mediated Effects on Hepatic Coenzyme A (CoA) and Acetyl-CoA Levels (part-I)","STUDY_TYPE":"Dioxin","STUDY_SUMMARY":"Experiment to study TCDD-elicited effects on hepatic Coenzyme A (CoA) and Acetyl-CoA levels in liver of male mice treated with sesame oil vehicle or 0.01-30 ug/kg TCDD every 4 days for 28 days.","INSTITUTE":"Michigan State University","DEPARTMENT":"Biochemistry and Molecular Biology","LABORATORY":"Zacharewski Lab","LAST_NAME":"Fling","FIRST_NAME":"Russell","ADDRESS":"1129 Farm Ln, East Lansing, MI, USA","EMAIL":"flingrus@msu.edu","PHONE":"(517) 884-2054","SUBMIT_DATE":"2020-04-03"}, "SUBJECT":{"SUBJECT_TYPE":"Mammalian Subjects","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6","AGE_OR_AGE_RANGE":"postnatal day 56","GENDER":"Male"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"10", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_086.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:14:13"} }, { "Subject ID":"-", "Sample ID":"10_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_086.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:14:13"} }, { "Subject ID":"-", "Sample ID":"6", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_024.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"5:33:29"} }, { "Subject ID":"-", "Sample ID":"6_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_024.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"5:33:29"} }, { "Subject ID":"-", "Sample ID":"7", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_035.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:09:03"} }, { "Subject ID":"-", "Sample ID":"7_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_035.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:09:03"} }, { "Subject ID":"-", "Sample ID":"8", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_056.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:53:34"} }, { "Subject ID":"-", "Sample ID":"8_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_056.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:53:34"} }, { "Subject ID":"-", "Sample ID":"9", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_078.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:41:53"} }, { "Subject ID":"-", "Sample ID":"9_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_078.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:41:53"} }, { "Subject ID":"-", "Sample ID":"17", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_039.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:12:58"} }, { "Subject ID":"-", "Sample ID":"17_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_039.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:12:58"} }, { "Subject ID":"-", "Sample ID":"18", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_050.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:11:47"} }, { "Subject ID":"-", "Sample ID":"18_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_050.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:11:47"} }, { "Subject ID":"-", "Sample ID":"19", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_074.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:45:09"} }, { "Subject ID":"-", "Sample ID":"19_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_074.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:45:09"} }, { "Subject ID":"-", "Sample ID":"20", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_094.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:18:10"} }, { "Subject ID":"-", "Sample ID":"20_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_094.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:18:10"} }, { "Subject ID":"-", "Sample ID":"26", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_018.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"5:41:25"} }, { "Subject ID":"-", "Sample ID":"26_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_018.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"5:41:25"} }, { "Subject ID":"-", "Sample ID":"27", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_033.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:16:20"} }, { "Subject ID":"-", "Sample ID":"27_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_033.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:16:20"} }, { "Subject ID":"-", "Sample ID":"28", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_060.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:15:18"} }, { "Subject ID":"-", "Sample ID":"28_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_060.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:15:18"} }, { "Subject ID":"-", "Sample ID":"29", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_070.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:48:42"} }, { "Subject ID":"-", "Sample ID":"29_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_070.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:48:42"} }, { "Subject ID":"-", "Sample ID":"30", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_096.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:20:56"} }, { "Subject ID":"-", "Sample ID":"30_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_096.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:20:56"} }, { "Subject ID":"-", "Sample ID":"36", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_014.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"5:45:15"} }, { "Subject ID":"-", "Sample ID":"36_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_014.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"5:45:15"} }, { "Subject ID":"-", "Sample ID":"37", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_037.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:30:12"} }, { "Subject ID":"-", "Sample ID":"37_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_037.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:30:12"} }, { "Subject ID":"-", "Sample ID":"38", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_058.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:18:36"} }, { "Subject ID":"-", "Sample ID":"38_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_058.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:18:36"} }, { "Subject ID":"-", "Sample ID":"39", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_068.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:53:42"} }, { "Subject ID":"-", "Sample ID":"39_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_068.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:53:42"} }, { "Subject ID":"-", "Sample ID":"46", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_012.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"5:48:59"} }, { "Subject ID":"-", "Sample ID":"46_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_012.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"5:48:59"} }, { "Subject ID":"-", "Sample ID":"47", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_031.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:20:14"} }, { "Subject ID":"-", "Sample ID":"47_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_031.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:20:14"} }, { "Subject ID":"-", "Sample ID":"48", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_048.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:22:17"} }, { "Subject ID":"-", "Sample ID":"48_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_048.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:22:17"} }, { "Subject ID":"-", "Sample ID":"49", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_084.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:57:17"} }, { "Subject ID":"-", "Sample ID":"49_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_084.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:57:17"} }, { "Subject ID":"-", "Sample ID":"56", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_010.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"5:53:06"} }, { "Subject ID":"-", "Sample ID":"56_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_010.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"5:53:06"} }, { "Subject ID":"-", "Sample ID":"58", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_064.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:26:39"} }, { "Subject ID":"-", "Sample ID":"58_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_064.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:26:39"} }, { "Subject ID":"-", "Sample ID":"59", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_076.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:00:36"} }, { "Subject ID":"-", "Sample ID":"59_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_076.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:00:36"} }, { "Subject ID":"-", "Sample ID":"60", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_088.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:29:46"} }, { "Subject ID":"-", "Sample ID":"60_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_088.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:29:46"} }, { "Subject ID":"-", "Sample ID":"76", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_022.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:01:34"} }, { "Subject ID":"-", "Sample ID":"76_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_022.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:01:34"} }, { "Subject ID":"-", "Sample ID":"77", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_029.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:39:31"} }, { "Subject ID":"-", "Sample ID":"77_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_029.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:39:31"} }, { "Subject ID":"-", "Sample ID":"78", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_052.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:33:48"} }, { "Subject ID":"-", "Sample ID":"78_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_052.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:33:48"} }, { "Subject ID":"-", "Sample ID":"79", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_080.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:07:40"} }, { "Subject ID":"-", "Sample ID":"79_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_080.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:07:40"} }, { "Subject ID":"-", "Sample ID":"80", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_090.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:35:49"} }, { "Subject ID":"-", "Sample ID":"80_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_090.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:35:49"} }, { "Subject ID":"-", "Sample ID":"66", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_020.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"5:56:46"} }, { "Subject ID":"-", "Sample ID":"66_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_020.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"5:56:46"} }, { "Subject ID":"-", "Sample ID":"67", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_041.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:33:55"} }, { "Subject ID":"-", "Sample ID":"67_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_041.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:33:55"} }, { "Subject ID":"-", "Sample ID":"68", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_062.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:30:22"} }, { "Subject ID":"-", "Sample ID":"68_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_062.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:30:22"} }, { "Subject ID":"-", "Sample ID":"69", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_082.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:03:47"} }, { "Subject ID":"-", "Sample ID":"69_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_082.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:03:47"} }, { "Subject ID":"-", "Sample ID":"70", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_098.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:32:58"} }, { "Subject ID":"-", "Sample ID":"70_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_098.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:32:58"} }, { "Subject ID":"-", "Sample ID":"86", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_016.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:04:56"} }, { "Subject ID":"-", "Sample ID":"86_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_016.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:04:56"} }, { "Subject ID":"-", "Sample ID":"87", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_043.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"6:42:43"} }, { "Subject ID":"-", "Sample ID":"87_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_043.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"6:42:43"} }, { "Subject ID":"-", "Sample ID":"89", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_054.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"7:37:33"} }, { "Subject ID":"-", "Sample ID":"89_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_054.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"7:37:33"} }, { "Subject ID":"-", "Sample ID":"90", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_092.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"8:10:53"} }, { "Subject ID":"-", "Sample ID":"90_1uM_Coa_added", "Factors":{"Collection Time":"Afternoon","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_092.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"8:10:53"} }, { "Subject ID":"-", "Sample ID":"1", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_025.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:00:11"} }, { "Subject ID":"-", "Sample ID":"1_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_025.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:00:11"} }, { "Subject ID":"-", "Sample ID":"2", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_033.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:38:05"} }, { "Subject ID":"-", "Sample ID":"2_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_033.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:38:05"} }, { "Subject ID":"-", "Sample ID":"3", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_069.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:18:00"} }, { "Subject ID":"-", "Sample ID":"3_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_069.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:18:00"} }, { "Subject ID":"-", "Sample ID":"4", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_073.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:07:51"} }, { "Subject ID":"-", "Sample ID":"4_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_073.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:07:51"} }, { "Subject ID":"-", "Sample ID":"5", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_100.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:36:42"} }, { "Subject ID":"-", "Sample ID":"5_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"-"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_100.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:36:42"} }, { "Subject ID":"-", "Sample ID":"11", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_072.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:04:36"} }, { "Subject ID":"-", "Sample ID":"11_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_090519_072.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:04:36"} }, { "Subject ID":"-", "Sample ID":"13", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_061.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:22:18"} }, { "Subject ID":"-", "Sample ID":"13_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_061.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:22:18"} }, { "Subject ID":"-", "Sample ID":"15", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_104.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:11:10"} }, { "Subject ID":"-", "Sample ID":"15_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.01"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_104.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:11:10"} }, { "Subject ID":"-", "Sample ID":"21", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_027.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:09:35"} }, { "Subject ID":"-", "Sample ID":"21_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_027.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:09:35"} }, { "Subject ID":"-", "Sample ID":"22", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_039.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:46:22"} }, { "Subject ID":"-", "Sample ID":"22_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_039.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:46:22"} }, { "Subject ID":"-", "Sample ID":"23", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_055.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:26:34"} }, { "Subject ID":"-", "Sample ID":"23_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_055.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:26:34"} }, { "Subject ID":"-", "Sample ID":"24", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_084.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:14:25"} }, { "Subject ID":"-", "Sample ID":"24_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.03"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_084.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:14:25"} }, { "Subject ID":"-", "Sample ID":"31", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_019.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"-"} }, { "Subject ID":"-", "Sample ID":"31_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_019.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"-"} }, { "Subject ID":"-", "Sample ID":"32", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_047.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:49:55"} }, { "Subject ID":"-", "Sample ID":"32_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_047.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:49:55"} }, { "Subject ID":"-", "Sample ID":"33", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_051.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:47:11"} }, { "Subject ID":"-", "Sample ID":"33_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_051.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:47:11"} }, { "Subject ID":"-", "Sample ID":"34", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_086.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:18:44"} }, { "Subject ID":"-", "Sample ID":"34_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_086.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:18:44"} }, { "Subject ID":"-", "Sample ID":"35", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_090.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:40:07"} }, { "Subject ID":"-", "Sample ID":"35_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_090.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:40:07"} }, { "Subject ID":"-", "Sample ID":"41", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_031.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:18:21"} }, { "Subject ID":"-", "Sample ID":"41_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_031.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:18:21"} }, { "Subject ID":"-", "Sample ID":"42", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_045.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:53:55"} }, { "Subject ID":"-", "Sample ID":"42_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_045.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:53:55"} }, { "Subject ID":"-", "Sample ID":"43", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_067.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:51:07"} }, { "Subject ID":"-", "Sample ID":"43_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_067.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:51:07"} }, { "Subject ID":"-", "Sample ID":"44", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_088.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:22:26"} }, { "Subject ID":"-", "Sample ID":"44_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_088.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:22:26"} }, { "Subject ID":"-", "Sample ID":"45", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_096.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:43:01"} }, { "Subject ID":"-", "Sample ID":"45_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"0.3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_096.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:43:01"} }, { "Subject ID":"-", "Sample ID":"51", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_029.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:23:10"} }, { "Subject ID":"-", "Sample ID":"51_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_029.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:23:10"} }, { "Subject ID":"-", "Sample ID":"52", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_043.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:57:21"} }, { "Subject ID":"-", "Sample ID":"52_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_043.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:57:21"} }, { "Subject ID":"-", "Sample ID":"53", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_065.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:54:33"} }, { "Subject ID":"-", "Sample ID":"53_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_065.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:54:33"} }, { "Subject ID":"-", "Sample ID":"54", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_080.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:26:12"} }, { "Subject ID":"-", "Sample ID":"54_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_080.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:26:12"} }, { "Subject ID":"-", "Sample ID":"55", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_094.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:46:12"} }, { "Subject ID":"-", "Sample ID":"55_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"1"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_094.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:46:12"} }, { "Subject ID":"-", "Sample ID":"71", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_023.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:30:25"} }, { "Subject ID":"-", "Sample ID":"71_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_023.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:30:25"} }, { "Subject ID":"-", "Sample ID":"72", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_049.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:05:24"} }, { "Subject ID":"-", "Sample ID":"72_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_049.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:05:24"} }, { "Subject ID":"-", "Sample ID":"73", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_053.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:00:57"} }, { "Subject ID":"-", "Sample ID":"73_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_053.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:00:57"} }, { "Subject ID":"-", "Sample ID":"74", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_071.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:33:42"} }, { "Subject ID":"-", "Sample ID":"74_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_071.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:33:42"} }, { "Subject ID":"-", "Sample ID":"75", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_102.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:51:01"} }, { "Subject ID":"-", "Sample ID":"75_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"10"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_102.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:51:01"} }, { "Subject ID":"-", "Sample ID":"61", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_021.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:26:37"} }, { "Subject ID":"-", "Sample ID":"61_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_021.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:26:37"} }, { "Subject ID":"-", "Sample ID":"62", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_041.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:00:57"} }, { "Subject ID":"-", "Sample ID":"62_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_041.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:00:57"} }, { "Subject ID":"-", "Sample ID":"63", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_063.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:57:41"} }, { "Subject ID":"-", "Sample ID":"63_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_063.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:57:41"} }, { "Subject ID":"-", "Sample ID":"64", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_082.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:29:49"} }, { "Subject ID":"-", "Sample ID":"64_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_082.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:29:49"} }, { "Subject ID":"-", "Sample ID":"65", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_098.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:49:10"} }, { "Subject ID":"-", "Sample ID":"65_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"3"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_098.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:49:10"} }, { "Subject ID":"-", "Sample ID":"82", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_017.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"0:34:20"} }, { "Subject ID":"-", "Sample ID":"82_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_017.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"0:34:20"} }, { "Subject ID":"-", "Sample ID":"84", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_037.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"1:09:05"} }, { "Subject ID":"-", "Sample ID":"84_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_037.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"1:09:05"} }, { "Subject ID":"-", "Sample ID":"85", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_059.raw","CoA standard addition":"no CoA std. addition","collection time (zietgeber time (H:MM:SS)":"2:04:02"} }, { "Subject ID":"-", "Sample ID":"85_1uM_Coa_added", "Factors":{"Collection Time":"Morning","TCDD treatment(ug/kg)":"30"}, "Additional sample data":{"RAW_FILE_NAME":"XS1_082919_059.raw","CoA standard addition":"1 uM CoA std addition","collection time (zietgeber time (H:MM:SS)":"2:04:02"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Mice were take down with CO2. Livers were excised and immediatley frozen in liquid nitrogen. Collection time (Morning or Afternoon) denoted in sample factors refers to take-down time. Morning cohort samples were collected from when the light turned on (7 am, ZT0) to ZT3.5. Afternoon cohort samples were collected from ZT5-8.5.","COLLECTION_PROTOCOL_COMMENTS":"Time-point: 28; Time-point Unit: Days;","SAMPLE_TYPE":"Liver","STORAGE_CONDITIONS":"-80℃"}, "TREATMENT":{"TREATMENT_SUMMARY":"oral gavage every 4 days for 28 days. 7 total exposures oral gavage every 4 days for 28 days. 7 total exposures with vehicle or 0.01-30 ug/kg TCDD.","TREATMENT_PROTOCOL_COMMENTS":"Administration Interval: 4; Administration Interval Unit: DAYS; Dose Unit: ug/kg; Number of Administrations: 7; Chemical Soucre: AccuStandard; Chemical Catalog Number: S-70982; Chemical Purity: -; Vehicle Substance: CHEBI 28119","TREATMENT_COMPOUND":"2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)","TREATMENT_ROUTE":"GAVAGE","TREATMENT_DOSE":"0.01,0.03,0.1,0.3,1,3,10,30","TREATMENT_DOSEVOLUME":"0.1 ml","TREATMENT_DOSEDURATION":"28 DAYS;","TREATMENT_VEHICLE":"Sesame oil"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Frozen liver tissue was homegenized in 1000 ul h20:methanol (5:3) mixture5 ml and homegenized, 625 ul chloroform was added,vortexed and spun down at 15,000G. 800 ul of top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section.","SAMPLEPREP_PROTOCOL_FILENAME":"SP_190815_Acetyl-CoA_modified_Metabolomics_polarextraction.docx","PROCESSING_METHOD":"Liquid extraction","PROCESSING_STORAGE_CONDITIONS":"-80 C"}, "CHROMATOGRAPHY":{"METHODS_FILENAME":"CH_190829_prj155_28RDDR_LC-MS_expiriment_settings_Xevo_G2-XS_QTof_NEG-mode.txt","INSTRUMENT_NAME":"Acquity H (Waters)","COLUMN_NAME":"Ascentis Express C18 2.1x5cm (Waters)","INTERNAL_STANDARD":"Acetyl-1,2-13C2 Coenzyme A Sodium Salt (A172214, Toronto Research Chemicals, Toronto, Canada)","CHROMATOGRAPHY_TYPE":"Reversed phase"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"MS_170814_prj140_28RDDR_liver_compound_measurements_QTOF1_NEG.txt"}, "MS":{"INSTRUMENT_NAME":"Waters Xevo G2 XS QTof","INSTRUMENT_TYPE":"TOF","MS_TYPE":"ESI","MS_COMMENTS":"Samples were run in MS^e mode. MassLynx V4.2 was used to measure area under the curve. Accurate mass and retention were used for identification of signal for respective metabolites.","ION_MODE":"NEGATIVE"}, "MS_METABOLITE_DATA":{ "Units":"nmol/mg total protein", "Data":[{"Metabolite":"acetyl-coa","10":"2.2159","6":"2.3828","7":"1.5989","8":"2.1954","9":"2.4543","17":"1.8728","18":"2.6342","19":"1.5160","20":"1.4476","26":"1.6000","27":"1.1697","28":"2.1707","29":"1.9352","30":"2.5440","36":"2.0520","37":"1.4957","38":"1.6076","39":"2.9079","46":"2.7142","47":"1.2730","48":"3.6566","49":"1.9934","56":"3.8692","58":"2.2475","59":"2.3399","60":"2.4181","76":"1.2302","77":"1.9557","78":"1.5453","79":"1.3701","80":"0.8614","66":"1.6225","67":"1.1968","68":"1.8670","69":"2.0662","70":"2.0846","87":"0.6247","89":"0.8056","90":"0.5218","1":"1.4942","2":"2.4709","3":"2.4694","4":"3.1688","5":"3.8684","11":"1.6411","13":"3.5639","15":"3.0476","21":"2.8214","22":"2.1784","23":"3.0725","24":"3.1436","31":"2.3040","32":"1.6865","33":"4.3636","34":"2.6944","35":"3.0662","41":"3.2521","42":"1.8382","43":"3.5370","44":"2.7930","45":"2.0757","51":"2.4859","52":"1.6832","53":"2.5705","54":"2.4549","55":"1.8792","71":"2.3039","72":"1.5166","73":"2.0303","74":"1.7782","75":"2.2943","61":"2.7691","62":"1.8469","63":"3.2190","64":"3.2536","65":"2.6261","82":"0.8621","84":"0.5414","85":"0.7642"},{"Metabolite":"Coenzyme A","10":"10.0808","6":"7.0780","7":"11.5181","8":"6.2198","9":"9.5884","17":"11.7701","18":"6.1272","19":"11.6535","20":"12.3824","26":"10.1981","27":"14.4735","28":"7.8192","29":"11.5014","30":"14.9029","36":"12.2803","37":"10.1760","38":"10.7999","39":"10.6519","46":"12.9513","47":"14.1545","48":"5.6500","49":"11.7734","56":"12.4498","58":"7.8053","59":"13.9706","60":"11.1028","76":"10.0897","77":"11.5296","78":"11.2133","79":"13.0423","80":"16.0554","66":"8.0464","67":"15.4303","68":"10.0363","69":"14.5289","70":"10.7239","87":"9.1508","89":"8.4528","90":"4.6657","1":"6.0380","2":"4.8281","3":"8.4765","4":"6.2538","5":"15.9818","11":"15.5446","13":"13.1808","15":"13.2219","21":"11.4612","22":"8.8322","23":"18.3500","24":"12.7409","31":"10.8057","32":"11.2232","33":"9.5698","34":"13.8333","35":"13.4325","41":"14.5539","42":"14.5512","43":"25.9182","44":"22.2971","45":"16.3554","51":"22.4537","52":"18.1612","53":"18.2665","54":"15.6190","55":"13.0755","71":"16.0141","72":"26.2931","73":"10.8778","74":"17.1308","75":"16.3902","61":"17.0464","62":"13.7223","63":"13.8506","64":"23.4316","65":"19.6168","82":"7.0923","84":"3.6007","85":"6.9959"}], "Metabolites":[{"Metabolite":"acetyl-coa","pubchem_id":"444493","inchi_key":"","kegg_id":"C00024","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"809.126","":""},{"Metabolite":"Coenzyme A","pubchem_id":"6816","inchi_key":"","kegg_id":"C00010","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"767.115","":""}] } }