{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001387","ANALYSIS_ID":"AN002316","VERSION":"1","CREATED_ON":"June 2, 2020, 10:47 am"},

"PROJECT":{"PROJECT_TITLE":"Bat liver lipid profiles","PROJECT_SUMMARY":"In this study, we investigated changes in hepatic lipid profiles of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at an early stage (70 d) of infection with the etiological agent, Pseudogymnoascus destructans (Pd).","INSTITUTE":"Georgetown University","LAST_NAME":"Pannkuk","FIRST_NAME":"Evan","ADDRESS":"3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, 20057, USA","EMAIL":"elp44@georgetown.edu","PHONE":"2026875650"},

"STUDY":{"STUDY_TITLE":"Bat liver lipid profiles","STUDY_SUMMARY":"In this study, we investigated changes in hepatic lipid profiles of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at an early stage (70 d) of infection with the etiological agent, Pseudogymnoascus destructans (Pd).","INSTITUTE":"Georgetown University","LAST_NAME":"Pannkuk","FIRST_NAME":"Evan","ADDRESS":"3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, 20057, USA","EMAIL":"elp44@georgetown.edu","PHONE":"2026875650"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Myotis lucifugus","TAXONOMY_ID":"59463"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"MYLU",
"Sample ID":"POS_10",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_10.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_11",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_11.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_12",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_12.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_13",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_13.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_14",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_14.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_15",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_15.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_16",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_16.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_17",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_17.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_18",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_18.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_19",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_19.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_20",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_20.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_21",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_21.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_22",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_22.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_23",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_23.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_27",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_27.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_28",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_28.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_29",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_29.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_30",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_30.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_31",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_31.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_32",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_32.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_33",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_33.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_34",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_34.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_35",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_35.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_36",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_36.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_37",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_37.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_38",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_38.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_42",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_42.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_43",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_43.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_44",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_44.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_45",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_45.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_46",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_46.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_47",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_47.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_48",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_48.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_49",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_49.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_50",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_50.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_51",
"Factors":{"Treatment":"sham"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_51.raw"}
},
{
"Subject ID":"MYLU",
"Sample ID":"POS_52",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"m","RAW_FILE_NAME":"POS_52.raw"}
},
{
"Subject ID":"EPFU",
"Sample ID":"POS_53",
"Factors":{"Treatment":"Pd"},
"Additional sample data":{"Sex":"f","RAW_FILE_NAME":"POS_53.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Laboratory experiments were conducted at the University of Winnipeg (Manitoba Sustainable Development Species at Risk/Wildlife Scientific Permit # SAR16009). M. lucifugus were collected from a WNS-negative hibernaculum in Central Manitoba in January (mid-winter) 2017. E. fuscus were collected from a WNS-negative hibernaculum in northwestern Ontario near Kenora in the same month. Hibernating bats were removed from the cave wall by hand and transferred to a bio-secure bat facility as previously described (Warnecke et al., 2012; McGuire et al., 2016). Bats were tested to be negative for Pd upon arrival using standard qPCR techniques and inoculated using previously established protocols (Warnecke et al., 2012; McGuire et al., 2016).","SAMPLE_TYPE":"Liver"},

"TREATMENT":{"TREATMENT_SUMMARY":"Bats given sham (PBST) or Pd inoculation"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Liver samples (~10 mg) were homogenized with cold methanol (300 μl) containing internal standards (10 μl SPLASH® Lipidomix®), 10 μl was removed for protein quantification, and the tissue homogenate was incubated on ice for 5 min. Chilled chloroform (600 μl) was added to samples and followed by shaking (vortex 30 sec) and further incubation on ice (10 min). Chilled water (300 μl) was added to samples followed by shaking (vortex 30 sec) and incubation on ice (10 min), then centrifuged for 10 min (10,000 x g, 4°C) to separate layers. The lower organic phase was separated, evaporated under vacuum, and reconstituted in 200 μl isopropanol (IPA):acetonitrile (ACN):H2O (2:1:1) for analysis.","PROCESSING_STORAGE_CONDITIONS":"On ice","EXTRACT_STORAGE":"On ice"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity CSH C18 (100 x 2.1mm, 1.7um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Waters Synapt G2 S QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"None","MS_RESULTS_FILE":"ST001387_AN002316_Results.txt UNITS:m/z values Has m/z:Yes Has RT:Yes RT units:Minutes"}

}