{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001491","ANALYSIS_ID":"AN002471","VERSION":"1","CREATED_ON":"September 28, 2020, 12:31 pm"},

"PROJECT":{"PROJECT_TITLE":"Untargeted urine metabolomics to predict gestational age in term and preterm pregnancies","PROJECT_SUMMARY":"Multi-site collection of urine early in pregnancy (8-19 weeks) and untargeted LC-MS metabolomics to predict gestational age in term and preterm pregnancies","INSTITUTE":"Stanford University","DEPARTMENT":"Genetics","LAST_NAME":"Contrepois","FIRST_NAME":"Kevin","ADDRESS":"300 Pasteur Dr, ALWAY bldg M302, STANFORD, California, 94305, USA","EMAIL":"kcontrep@stanford.edu","PHONE":"6507239914"},

"STUDY":{"STUDY_TITLE":"Global Urine Metabolic Profiling to Predict Gestational Age in Term and Preterm Pregnancies","STUDY_SUMMARY":"Assessment of gestational age (GA) is key to provide optimal care during pregnancy. However, its accurate determination remains challenging in low- and middle-resource countries, where access to obstetric ultrasound is limited. Hence, there is an urgent need to develop clinical approaches that allow accurate and inexpensive estimation of GA. We investigated the ability of urinary metabolites to predict GA at time of collection in a diverse multi-site cohort (n = 99) using a broad-spectrum liquid chromatography coupled with mass spectrometry (LC-MS) platform. Our approach detected a myriad of steroid hormones and their derivatives including estrogens, progesterones, corticosteroids and androgens that associated with pregnancy progression. We developed a prediction model that predicted GA with high accuracy using the levels of three metabolites (rho = 0.87, .RMSE = 1.58 weeks). These predictions were robust irrespective of whether the pregnancy went to term or ended prematurely. Overall, we demonstrate the feasibility of implementing urine collection for metabolomics analysis in large-scale multi-site studies and we report a predictive model of GA with a potential clinical value.","INSTITUTE":"Stanford University","LAST_NAME":"Contrepois","FIRST_NAME":"Kevin","ADDRESS":"300 Pasteur Dr","EMAIL":"kcontrep@stanford.edu","PHONE":"6506664538"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENDER":"Female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"30-06183",
"Sample ID":"1",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_1;nHILIC_1;pRPLC_1 nRPLC_1"}
},
{
"Subject ID":"20UE01401",
"Sample ID":"2",
"Factors":{"Site":"Tanzania","GA_delivery":"31","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_2;nHILIC_2;pRPLC_2 nRPLC_2"}
},
{
"Subject ID":"20UE00635",
"Sample ID":"3",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_3;nHILIC_3;pRPLC_3 nRPLC_3"}
},
{
"Subject ID":"20-37618",
"Sample ID":"4",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"33","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_4;nHILIC_4;pRPLC_4 nRPLC_4"}
},
{
"Subject ID":"20UE01817",
"Sample ID":"5",
"Factors":{"Site":"Tanzania","GA_delivery":"32","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_5;nHILIC_5;pRPLC_5 nRPLC_5"}
},
{
"Subject ID":"20UE02184",
"Sample ID":"6",
"Factors":{"Site":"Tanzania","GA_delivery":"33","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_6;nHILIC_6;pRPLC_6 nRPLC_6"}
},
{
"Subject ID":"17-UE00413",
"Sample ID":"7",
"Factors":{"Site":"Pakistan","GA_delivery":"28","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_7;nHILIC_7;pRPLC_7 nRPLC_7"}
},
{
"Subject ID":"30-08014",
"Sample ID":"8",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"33","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_8;nHILIC_8;pRPLC_8 nRPLC_8"}
},
{
"Subject ID":"17-UE00679",
"Sample ID":"9",
"Factors":{"Site":"Pakistan","GA_delivery":"40","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_9;nHILIC_9;pRPLC_9 nRPLC_9"}
},
{
"Subject ID":"20UE00867",
"Sample ID":"10",
"Factors":{"Site":"Tanzania","GA_delivery":"26","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_10;nHILIC_10;pRPLC_10 nRPLC_10"}
},
{
"Subject ID":"17-UE01059",
"Sample ID":"11",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_11;nHILIC_11;pRPLC_11 nRPLC_11"}
},
{
"Subject ID":"11UE01127",
"Sample ID":"12",
"Factors":{"Site":"Bangladesh","GA_delivery":"33","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_12;nHILIC_12;pRPLC_12 nRPLC_12"}
},
{
"Subject ID":"30-06482",
"Sample ID":"13",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_13;nHILIC_13;pRPLC_13 nRPLC_13"}
},
{
"Subject ID":"11UE00971",
"Sample ID":"14",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_14;nHILIC_14;pRPLC_14 nRPLC_14"}
},
{
"Subject ID":"17-UE00922",
"Sample ID":"15",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_15;nHILIC_15;pRPLC_15 nRPLC_15"}
},
{
"Subject ID":"20UE00071",
"Sample ID":"16",
"Factors":{"Site":"Tanzania","GA_delivery":"33","GA_sampling":"14"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_16;nHILIC_16;pRPLC_16 nRPLC_16"}
},
{
"Subject ID":"20-27695",
"Sample ID":"17",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"33","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_17;nHILIC_17;pRPLC_17 nRPLC_17"}
},
{
"Subject ID":"17-UE00283",
"Sample ID":"18",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_18;nHILIC_18;pRPLC_18 nRPLC_18"}
},
{
"Subject ID":"11UE00066",
"Sample ID":"19",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_19;nHILIC_19;pRPLC_19 nRPLC_19"}
},
{
"Subject ID":"11UE01855",
"Sample ID":"20",
"Factors":{"Site":"Bangladesh","GA_delivery":"29","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_20;nHILIC_20;pRPLC_20 nRPLC_20"}
},
{
"Subject ID":"20UE02184",
"Sample ID":"21",
"Factors":{"Site":"Tanzania","GA_delivery":"33","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_21;nHILIC_21;pRPLC_21 nRPLC_21"}
},
{
"Subject ID":"11UE00631",
"Sample ID":"22",
"Factors":{"Site":"Bangladesh","GA_delivery":"30","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_22;nHILIC_22;pRPLC_22 nRPLC_22"}
},
{
"Subject ID":"20-01475",
"Sample ID":"23",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"39","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_23;nHILIC_23;pRPLC_23 nRPLC_23"}
},
{
"Subject ID":"30-00423",
"Sample ID":"24",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"41","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_24;nHILIC_24;pRPLC_24 nRPLC_24"}
},
{
"Subject ID":"20UE00285",
"Sample ID":"25",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_25;nHILIC_25;pRPLC_25 nRPLC_25"}
},
{
"Subject ID":"11UE00066",
"Sample ID":"26",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_26;nHILIC_26;pRPLC_26 nRPLC_26"}
},
{
"Subject ID":"20UE00701",
"Sample ID":"27",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_27;nHILIC_27;pRPLC_27 nRPLC_27"}
},
{
"Subject ID":"30-07271",
"Sample ID":"28",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"31","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_28;nHILIC_28;pRPLC_28 nRPLC_28"}
},
{
"Subject ID":"11UE00848",
"Sample ID":"29",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_29;nHILIC_29;pRPLC_29 nRPLC_29"}
},
{
"Subject ID":"20-23828",
"Sample ID":"30",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"34","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_30;nHILIC_30;pRPLC_30 nRPLC_30"}
},
{
"Subject ID":"17-UE00384",
"Sample ID":"31",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_31;nHILIC_31;pRPLC_31 nRPLC_31"}
},
{
"Subject ID":"20UE00691",
"Sample ID":"32",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_32;nHILIC_32;pRPLC_32 nRPLC_32"}
},
{
"Subject ID":"17-UE00283",
"Sample ID":"33",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_33;nHILIC_33;pRPLC_33 nRPLC_33"}
},
{
"Subject ID":"17-UE01072",
"Sample ID":"34",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_34;nHILIC_34;pRPLC_34 nRPLC_34"}
},
{
"Subject ID":"20UE01639",
"Sample ID":"35",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_35;nHILIC_35;pRPLC_35 nRPLC_35"}
},
{
"Subject ID":"30-00472",
"Sample ID":"36",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"28","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_36;nHILIC_36;pRPLC_36 nRPLC_36"}
},
{
"Subject ID":"17-UE00922",
"Sample ID":"37",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_37;nHILIC_37;pRPLC_37 nRPLC_37"}
},
{
"Subject ID":"20UE00071",
"Sample ID":"38",
"Factors":{"Site":"Tanzania","GA_delivery":"33","GA_sampling":"14"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_38;nHILIC_38;pRPLC_38 nRPLC_38"}
},
{
"Subject ID":"20-37431",
"Sample ID":"39",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"33","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_39;nHILIC_39;pRPLC_39 nRPLC_39"}
},
{
"Subject ID":"20UE00700",
"Sample ID":"40",
"Factors":{"Site":"Tanzania","GA_delivery":"26","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_40;nHILIC_40;pRPLC_40 nRPLC_40"}
},
{
"Subject ID":"17-UE00848",
"Sample ID":"41",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_41;nHILIC_41;pRPLC_41 nRPLC_41"}
},
{
"Subject ID":"11UE00742",
"Sample ID":"42",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_42;nHILIC_42;pRPLC_42 nRPLC_42"}
},
{
"Subject ID":"20-01444",
"Sample ID":"43",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_43;nHILIC_43;pRPLC_43 nRPLC_43"}
},
{
"Subject ID":"30-08712",
"Sample ID":"44",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"36","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_44;nHILIC_44;pRPLC_44 nRPLC_44"}
},
{
"Subject ID":"11UE01104",
"Sample ID":"45",
"Factors":{"Site":"Bangladesh","GA_delivery":"29","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_45;nHILIC_45;pRPLC_45 nRPLC_45"}
},
{
"Subject ID":"30-05663",
"Sample ID":"46",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_46;nHILIC_46;pRPLC_46 nRPLC_46"}
},
{
"Subject ID":"11UE00631",
"Sample ID":"47",
"Factors":{"Site":"Bangladesh","GA_delivery":"30","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_47;nHILIC_47;pRPLC_47 nRPLC_47"}
},
{
"Subject ID":"11UE00742",
"Sample ID":"48",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_48;nHILIC_48;pRPLC_48 nRPLC_48"}
},
{
"Subject ID":"20UE02238",
"Sample ID":"49",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_49;nHILIC_49;pRPLC_49 nRPLC_49"}
},
{
"Subject ID":"11UE00455",
"Sample ID":"50",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_50;nHILIC_50;pRPLC_50 nRPLC_50"}
},
{
"Subject ID":"30-00423",
"Sample ID":"51",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"41","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_51;nHILIC_51;pRPLC_51 nRPLC_51"}
},
{
"Subject ID":"17-UE00374",
"Sample ID":"52",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_52;nHILIC_52;pRPLC_52 nRPLC_52"}
},
{
"Subject ID":"17-UE00384",
"Sample ID":"53",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_53;nHILIC_53;pRPLC_53 nRPLC_53"}
},
{
"Subject ID":"30-04509",
"Sample ID":"54",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_54;nHILIC_54;pRPLC_54 nRPLC_54"}
},
{
"Subject ID":"17-UE00208",
"Sample ID":"55",
"Factors":{"Site":"Pakistan","GA_delivery":"32","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_55;nHILIC_55;pRPLC_55 nRPLC_55"}
},
{
"Subject ID":"30-05696",
"Sample ID":"56",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"32","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_56;nHILIC_56;pRPLC_56 nRPLC_56"}
},
{
"Subject ID":"30-04510",
"Sample ID":"57",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"28","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_57;nHILIC_57;pRPLC_57 nRPLC_57"}
},
{
"Subject ID":"20-20993",
"Sample ID":"58",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"36","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_58;nHILIC_58;pRPLC_58 nRPLC_58"}
},
{
"Subject ID":"11UE01294",
"Sample ID":"59",
"Factors":{"Site":"Bangladesh","GA_delivery":"24","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_59;nHILIC_59;pRPLC_59 nRPLC_59"}
},
{
"Subject ID":"20UE00700",
"Sample ID":"60",
"Factors":{"Site":"Tanzania","GA_delivery":"26","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_60;nHILIC_60;pRPLC_60 nRPLC_60"}
},
{
"Subject ID":"11UE02004",
"Sample ID":"61",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_61;nHILIC_61;pRPLC_61 nRPLC_61"}
},
{
"Subject ID":"20-30636",
"Sample ID":"62",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_62;nHILIC_62;pRPLC_62 nRPLC_62"}
},
{
"Subject ID":"20UE01401",
"Sample ID":"63",
"Factors":{"Site":"Tanzania","GA_delivery":"31","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_63;nHILIC_63;pRPLC_63 nRPLC_63"}
},
{
"Subject ID":"20-20311",
"Sample ID":"64",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_64;nHILIC_64;pRPLC_64 nRPLC_64"}
},
{
"Subject ID":"20UE00754",
"Sample ID":"65",
"Factors":{"Site":"Tanzania","GA_delivery":"26","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_65;nHILIC_65;pRPLC_65 nRPLC_65"}
},
{
"Subject ID":"17-UE01243",
"Sample ID":"66",
"Factors":{"Site":"Pakistan","GA_delivery":"32","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_66;nHILIC_66;pRPLC_66 nRPLC_66"}
},
{
"Subject ID":"17-UE00789",
"Sample ID":"67",
"Factors":{"Site":"Pakistan","GA_delivery":"32","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_67;nHILIC_67;pRPLC_67 nRPLC_67"}
},
{
"Subject ID":"20UE01240",
"Sample ID":"68",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_68;nHILIC_68;pRPLC_68 nRPLC_68"}
},
{
"Subject ID":"17-UE00208",
"Sample ID":"69",
"Factors":{"Site":"Pakistan","GA_delivery":"32","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_69;nHILIC_69;pRPLC_69 nRPLC_69"}
},
{
"Subject ID":"11UE00374",
"Sample ID":"70",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_70;nHILIC_70;pRPLC_70 nRPLC_70"}
},
{
"Subject ID":"30-07165",
"Sample ID":"71",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"31","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_71;nHILIC_71;pRPLC_71 nRPLC_71"}
},
{
"Subject ID":"17-UE00401",
"Sample ID":"72",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_72;nHILIC_72;pRPLC_72 nRPLC_72"}
},
{
"Subject ID":"11UE01476",
"Sample ID":"73",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_73;nHILIC_73;pRPLC_73 nRPLC_73"}
},
{
"Subject ID":"11UE00490",
"Sample ID":"74",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_74;nHILIC_74;pRPLC_74 nRPLC_74"}
},
{
"Subject ID":"20UE01639",
"Sample ID":"75",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_75;nHILIC_75;pRPLC_75 nRPLC_75"}
},
{
"Subject ID":"11UE00971",
"Sample ID":"76",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_76;nHILIC_76;pRPLC_76 nRPLC_76"}
},
{
"Subject ID":"30-06436",
"Sample ID":"77",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"35","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_77;nHILIC_77;pRPLC_77 nRPLC_77"}
},
{
"Subject ID":"11UE01266",
"Sample ID":"78",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_78;nHILIC_78;pRPLC_78 nRPLC_78"}
},
{
"Subject ID":"20UE00157",
"Sample ID":"79",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"14"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_79;nHILIC_79;pRPLC_79 nRPLC_79"}
},
{
"Subject ID":"17-UE00789",
"Sample ID":"80",
"Factors":{"Site":"Pakistan","GA_delivery":"32","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_80;nHILIC_80;pRPLC_80 nRPLC_80"}
},
{
"Subject ID":"11UE01104",
"Sample ID":"81",
"Factors":{"Site":"Bangladesh","GA_delivery":"29","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_81;nHILIC_81;pRPLC_81 nRPLC_81"}
},
{
"Subject ID":"17-UE01047",
"Sample ID":"82",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_82;nHILIC_82;pRPLC_82 nRPLC_82"}
},
{
"Subject ID":"17-UE00394",
"Sample ID":"83",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_83;nHILIC_83;pRPLC_83 nRPLC_83"}
},
{
"Subject ID":"20UE02257",
"Sample ID":"84",
"Factors":{"Site":"Tanzania","GA_delivery":"33","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_84;nHILIC_84;pRPLC_84 nRPLC_84"}
},
{
"Subject ID":"20UE00867",
"Sample ID":"85",
"Factors":{"Site":"Tanzania","GA_delivery":"26","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_85;nHILIC_85;pRPLC_85 nRPLC_85"}
},
{
"Subject ID":"30-00472",
"Sample ID":"86",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"28","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_86;nHILIC_86;pRPLC_86 nRPLC_86"}
},
{
"Subject ID":"20UE01197",
"Sample ID":"87",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_87;nHILIC_87;pRPLC_87 nRPLC_87"}
},
{
"Subject ID":"20-23895",
"Sample ID":"88",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_88;nHILIC_88;pRPLC_88 nRPLC_88"}
},
{
"Subject ID":"11UE01121",
"Sample ID":"89",
"Factors":{"Site":"Bangladesh","GA_delivery":"29","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_89;nHILIC_89;pRPLC_89 nRPLC_89"}
},
{
"Subject ID":"30-07113",
"Sample ID":"90",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_90;nHILIC_90;pRPLC_90 nRPLC_90"}
},
{
"Subject ID":"30-00472",
"Sample ID":"91",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"28","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_91;nHILIC_91;pRPLC_91 nRPLC_91"}
},
{
"Subject ID":"11UE00904",
"Sample ID":"92",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_92;nHILIC_92;pRPLC_92 nRPLC_92"}
},
{
"Subject ID":"20UE00831",
"Sample ID":"93",
"Factors":{"Site":"Tanzania","GA_delivery":"32","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_93;nHILIC_93;pRPLC_93 nRPLC_93"}
},
{
"Subject ID":"20-32883",
"Sample ID":"94",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"39","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_94;nHILIC_94;pRPLC_94 nRPLC_94"}
},
{
"Subject ID":"11UE01391",
"Sample ID":"95",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_95;nHILIC_95;pRPLC_95 nRPLC_95"}
},
{
"Subject ID":"20-30035",
"Sample ID":"96",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"35","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_96;nHILIC_96;pRPLC_96 nRPLC_96"}
},
{
"Subject ID":"17-UE00873",
"Sample ID":"97",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_97;nHILIC_97;pRPLC_97 nRPLC_97"}
},
{
"Subject ID":"20UE00285",
"Sample ID":"98",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_98;nHILIC_98;pRPLC_98 nRPLC_98"}
},
{
"Subject ID":"30-01421",
"Sample ID":"99",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"37","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_99;nHILIC_99;pRPLC_99 nRPLC_99"}
},
{
"Subject ID":"20-23876",
"Sample ID":"100",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"36","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_100;nHILIC_100;pRPLC_100 nRPLC_100"}
},
{
"Subject ID":"20UE00701",
"Sample ID":"101",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_101;nHILIC_101;pRPLC_101 nRPLC_101"}
},
{
"Subject ID":"11UE00848",
"Sample ID":"102",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_102;nHILIC_102;pRPLC_102 nRPLC_102"}
},
{
"Subject ID":"30-01421",
"Sample ID":"103",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"37","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_103;nHILIC_103;pRPLC_103 nRPLC_103"}
},
{
"Subject ID":"17-UE00787",
"Sample ID":"104",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_104;nHILIC_104;pRPLC_104 nRPLC_104"}
},
{
"Subject ID":"11UE01266",
"Sample ID":"105",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_105;nHILIC_105;pRPLC_105 nRPLC_105"}
},
{
"Subject ID":"11UE01876",
"Sample ID":"106",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_106;nHILIC_106;pRPLC_106 nRPLC_106"}
},
{
"Subject ID":"17-UE00374",
"Sample ID":"107",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_107;nHILIC_107;pRPLC_107 nRPLC_107"}
},
{
"Subject ID":"20UE00635",
"Sample ID":"108",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_108;nHILIC_108;pRPLC_108 nRPLC_108"}
},
{
"Subject ID":"17-UE00848",
"Sample ID":"109",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_109;nHILIC_109;pRPLC_109 nRPLC_109"}
},
{
"Subject ID":"20UE02100",
"Sample ID":"110",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_110;nHILIC_110;pRPLC_110 nRPLC_110"}
},
{
"Subject ID":"20UE00754",
"Sample ID":"111",
"Factors":{"Site":"Tanzania","GA_delivery":"26","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_111;nHILIC_111;pRPLC_111 nRPLC_111"}
},
{
"Subject ID":"11UE00099",
"Sample ID":"112",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_112;nHILIC_112;pRPLC_112 nRPLC_112"}
},
{
"Subject ID":"20-27607",
"Sample ID":"113",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"32","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_113;nHILIC_113;pRPLC_113 nRPLC_113"}
},
{
"Subject ID":"11UE00455",
"Sample ID":"114",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_114;nHILIC_114;pRPLC_114 nRPLC_114"}
},
{
"Subject ID":"20UE01197",
"Sample ID":"115",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_115;nHILIC_115;pRPLC_115 nRPLC_115"}
},
{
"Subject ID":"30-06158",
"Sample ID":"116",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"14"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_116;nHILIC_116;pRPLC_116 nRPLC_116"}
},
{
"Subject ID":"30-00423",
"Sample ID":"117",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"41","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_117;nHILIC_117;pRPLC_117 nRPLC_117"}
},
{
"Subject ID":"20-34360",
"Sample ID":"118",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"29","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_118;nHILIC_118;pRPLC_118 nRPLC_118"}
},
{
"Subject ID":"17-UE00873",
"Sample ID":"119",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_119;nHILIC_119;pRPLC_119 nRPLC_119"}
},
{
"Subject ID":"17-UE01072",
"Sample ID":"120",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_120;nHILIC_120;pRPLC_120 nRPLC_120"}
},
{
"Subject ID":"30-07248",
"Sample ID":"121",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_121;nHILIC_121;pRPLC_121 nRPLC_121"}
},
{
"Subject ID":"11UE01876",
"Sample ID":"122",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_122;nHILIC_122;pRPLC_122 nRPLC_122"}
},
{
"Subject ID":"20UE00691",
"Sample ID":"123",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_123;nHILIC_123;pRPLC_123 nRPLC_123"}
},
{
"Subject ID":"17-UE00253",
"Sample ID":"124",
"Factors":{"Site":"Pakistan","GA_delivery":"40","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_124;nHILIC_124;pRPLC_124 nRPLC_124"}
},
{
"Subject ID":"20UE02238",
"Sample ID":"125",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_125;nHILIC_125;pRPLC_125 nRPLC_125"}
},
{
"Subject ID":"11UE01121",
"Sample ID":"126",
"Factors":{"Site":"Bangladesh","GA_delivery":"29","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_126;nHILIC_126;pRPLC_126 nRPLC_126"}
},
{
"Subject ID":"17-UE00563",
"Sample ID":"127",
"Factors":{"Site":"Pakistan","GA_delivery":"40","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_127;nHILIC_127;pRPLC_127 nRPLC_127"}
},
{
"Subject ID":"20UE00831",
"Sample ID":"128",
"Factors":{"Site":"Tanzania","GA_delivery":"32","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_128;nHILIC_128;pRPLC_128 nRPLC_128"}
},
{
"Subject ID":"20UE01817",
"Sample ID":"129",
"Factors":{"Site":"Tanzania","GA_delivery":"32","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_129;nHILIC_129;pRPLC_129 nRPLC_129"}
},
{
"Subject ID":"30-00472",
"Sample ID":"130",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"28","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_130;nHILIC_130;pRPLC_130 nRPLC_130"}
},
{
"Subject ID":"20UE02257",
"Sample ID":"131",
"Factors":{"Site":"Tanzania","GA_delivery":"33","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_131;nHILIC_131;pRPLC_131 nRPLC_131"}
},
{
"Subject ID":"20-20374",
"Sample ID":"132",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_132;nHILIC_132;pRPLC_132 nRPLC_132"}
},
{
"Subject ID":"30-07248",
"Sample ID":"133",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_133;nHILIC_133;pRPLC_133 nRPLC_133"}
},
{
"Subject ID":"17-UE00253",
"Sample ID":"134",
"Factors":{"Site":"Pakistan","GA_delivery":"40","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_134;nHILIC_134;pRPLC_134 nRPLC_134"}
},
{
"Subject ID":"11UE00490",
"Sample ID":"135",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_135;nHILIC_135;pRPLC_135 nRPLC_135"}
},
{
"Subject ID":"30-07113",
"Sample ID":"136",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_136;nHILIC_136;pRPLC_136 nRPLC_136"}
},
{
"Subject ID":"11UE01391",
"Sample ID":"137",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_137;nHILIC_137;pRPLC_137 nRPLC_137"}
},
{
"Subject ID":"20UE02136",
"Sample ID":"138",
"Factors":{"Site":"Tanzania","GA_delivery":"32","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_138;nHILIC_138;pRPLC_138 nRPLC_138"}
},
{
"Subject ID":"17-UE00563",
"Sample ID":"139",
"Factors":{"Site":"Pakistan","GA_delivery":"40","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_139;nHILIC_139;pRPLC_139 nRPLC_139"}
},
{
"Subject ID":"17-UE00413",
"Sample ID":"140",
"Factors":{"Site":"Pakistan","GA_delivery":"28","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_140;nHILIC_140;pRPLC_140 nRPLC_140"}
},
{
"Subject ID":"20UE02100",
"Sample ID":"141",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_141;nHILIC_141;pRPLC_141 nRPLC_141"}
},
{
"Subject ID":"30-04431",
"Sample ID":"142",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"40","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_142;nHILIC_142;pRPLC_142 nRPLC_142"}
},
{
"Subject ID":"20-29287",
"Sample ID":"143",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_143;nHILIC_143;pRPLC_143 nRPLC_143"}
},
{
"Subject ID":"17-UE00401",
"Sample ID":"144",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_144;nHILIC_144;pRPLC_144 nRPLC_144"}
},
{
"Subject ID":"20UE02136",
"Sample ID":"145",
"Factors":{"Site":"Tanzania","GA_delivery":"32","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_145;nHILIC_145;pRPLC_145 nRPLC_145"}
},
{
"Subject ID":"11UE01855",
"Sample ID":"146",
"Factors":{"Site":"Bangladesh","GA_delivery":"29","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_146;nHILIC_146;pRPLC_146 nRPLC_146"}
},
{
"Subject ID":"17-UE01059",
"Sample ID":"147",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_147;nHILIC_147;pRPLC_147 nRPLC_147"}
},
{
"Subject ID":"30-00423",
"Sample ID":"148",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"41","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_148;nHILIC_148;pRPLC_148 nRPLC_148"}
},
{
"Subject ID":"30-07165",
"Sample ID":"149",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"31","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_149;nHILIC_149;pRPLC_149 nRPLC_149"}
},
{
"Subject ID":"30-08712",
"Sample ID":"150",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"36","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_150;nHILIC_150;pRPLC_150 nRPLC_150"}
},
{
"Subject ID":"30-07249",
"Sample ID":"151",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"33","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_151;nHILIC_151;pRPLC_151 nRPLC_151"}
},
{
"Subject ID":"20UE00157",
"Sample ID":"152",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"14"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_152;nHILIC_152;pRPLC_152 nRPLC_152"}
},
{
"Subject ID":"11UE00904",
"Sample ID":"153",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_153;nHILIC_153;pRPLC_153 nRPLC_153"}
},
{
"Subject ID":"20-31519",
"Sample ID":"154",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_154;nHILIC_154;pRPLC_154 nRPLC_154"}
},
{
"Subject ID":"17-UE01243",
"Sample ID":"155",
"Factors":{"Site":"Pakistan","GA_delivery":"32","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_155;nHILIC_155;pRPLC_155 nRPLC_155"}
},
{
"Subject ID":"20UE01240",
"Sample ID":"156",
"Factors":{"Site":"Tanzania","GA_delivery":"39","GA_sampling":"19"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_156;nHILIC_156;pRPLC_156 nRPLC_156"}
},
{
"Subject ID":"20-20961",
"Sample ID":"157",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"40","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_157;nHILIC_157;pRPLC_157 nRPLC_157"}
},
{
"Subject ID":"20-02340",
"Sample ID":"158",
"Factors":{"Site":"Bangladesh_GAPPS","GA_delivery":"33","GA_sampling":"12"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_158;nHILIC_158;pRPLC_158 nRPLC_158"}
},
{
"Subject ID":"11UE00099",
"Sample ID":"159",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_159;nHILIC_159;pRPLC_159 nRPLC_159"}
},
{
"Subject ID":"17-UE01047",
"Sample ID":"160",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"10"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_160;nHILIC_160;pRPLC_160 nRPLC_160"}
},
{
"Subject ID":"17-UE01292",
"Sample ID":"161",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_161;nHILIC_161;pRPLC_161 nRPLC_161"}
},
{
"Subject ID":"11UE01294",
"Sample ID":"162",
"Factors":{"Site":"Bangladesh","GA_delivery":"24","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_162;nHILIC_162;pRPLC_162 nRPLC_162"}
},
{
"Subject ID":"11UE02004",
"Sample ID":"163",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"15"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_163;nHILIC_163;pRPLC_163 nRPLC_163"}
},
{
"Subject ID":"17-UE00679",
"Sample ID":"164",
"Factors":{"Site":"Pakistan","GA_delivery":"40","GA_sampling":"16"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_164;nHILIC_164;pRPLC_164 nRPLC_164"}
},
{
"Subject ID":"11UE01127",
"Sample ID":"165",
"Factors":{"Site":"Bangladesh","GA_delivery":"33","GA_sampling":"17"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_165;nHILIC_165;pRPLC_165 nRPLC_165"}
},
{
"Subject ID":"17-UE00394",
"Sample ID":"166",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"9"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_166;nHILIC_166;pRPLC_166 nRPLC_166"}
},
{
"Subject ID":"17-UE00787",
"Sample ID":"167",
"Factors":{"Site":"Pakistan","GA_delivery":"39","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_167;nHILIC_167;pRPLC_167 nRPLC_167"}
},
{
"Subject ID":"30-07249",
"Sample ID":"168",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"33","GA_sampling":"13"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_168;nHILIC_168;pRPLC_168 nRPLC_168"}
},
{
"Subject ID":"11UE01476",
"Sample ID":"169",
"Factors":{"Site":"Bangladesh","GA_delivery":"41","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_169;nHILIC_169;pRPLC_169 nRPLC_169"}
},
{
"Subject ID":"17-UE01292",
"Sample ID":"170",
"Factors":{"Site":"Pakistan","GA_delivery":"33","GA_sampling":"18"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_170;nHILIC_170;pRPLC_170 nRPLC_170"}
},
{
"Subject ID":"30-08014",
"Sample ID":"171",
"Factors":{"Site":"Zambia_GAPPS","GA_delivery":"31","GA_sampling":"11"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_171;nHILIC_171;pRPLC_171 nRPLC_171"}
},
{
"Subject ID":"11UE00374",
"Sample ID":"172",
"Factors":{"Site":"Bangladesh","GA_delivery":"31","GA_sampling":"8"},
"Additional sample data":{"RAW_FILE_NAME":"pHILIC_172;nHILIC_172;pRPLC_172 nRPLC_172"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"The study comprises a single urine sample for each participant (n = 99) that was collected at a prenatal visit after ultrasound confirmed a gestation < 20 weeks. Ultrasound imaging was performed by trained sonologists in compliance with standard-of-care. All study sites employed a uniform method of GA assessment, urine collection and handling. Urine samples were aliquoted and frozen at -80°C within 2 hours. Deidentified urine aliquots were shipped on dry ice from each biorepository to Stanford University as a single batch and under continuous temperature monitoring. Urine samples from 20 healthy pregnancies collected between 8 and 19 weeks of gestation at the Lucile Packard Children’s Hospital at Stanford University, served as the validation cohort.","SAMPLE_TYPE":"Urine","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"There was no treatment."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Urine aliquots were prepared and analyzed in a random order as previously described (Contrepois et al., 2015). Briefly, frozen urine samples were thawed on ice and centrifuged at 17,000g for 10 min at 4°C. Supernatants (25 µl) were then diluted 1:4 with 75% acetonitrile and 100% water for HILIC- and RPLC-MS experiments, respectively. Each sample was spiked-in with 15 analytical-grade internal standards (IS). Samples for HILIC-MS experiments were further centrifuged at 21,000g for 10 min at 4°C to precipitate proteins."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"HILIC experiments were performed using a ZIC-HILIC column 2.1x100 mm, 3.5μm, 200Å (Merck Millipore) and mobile phase solvents consisting of 10mM ammonium acetate in 50/50 acetonitrile/water (A) and 10 mM ammonium acetate in 95/5 acetonitrile/water (B).(Contrepois et al., 2015)","CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000 RS","COLUMN_NAME":"SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um)","FLOW_RATE":"0.5 ml/min","COLUMN_TEMPERATURE":"40","SOLVENT_A":"10mM ammonium acetate in 50/50 acetonitrile/water","SOLVENT_B":"10 mM ammonium acetate in 95/5 acetonitrile/water"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","OPERATOR_NAME":"Kevin Contrepois","DETECTOR_TYPE":"Orbitrap","DATA_FORMAT":".RAW"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Data processing. Data from each mode were independently analyzed using Progenesis QI software (v2.3) (Nonlinear Dynamics). Metabolic features from blanks and that did not show sufficient linearity upon dilution in QC samples (r < 0.6) were discarded. Only metabolic features present in > 2/3 of the samples were kept for further analysis. Inter- and intra-batch variations were corrected by applying locally estimated scatterplot smoothing local regression (LOESS) on pooled samples injected repetitively along the batches (span = 0.75). Data were acquired in four batches for HILIC and RPLC modes. Dilution effects were corrected using probabilistic quotient normalization (PQN) (Rosen Vollmar et al., 2019). Missing values were imputed by drawing from a random distribution of low values in the corresponding sample. Data from each mode were then merged, producing a dataset containing 6,630 metabolic features. Metabolite abundances were reported as spectral counts. Metabolic feature annotation. Peak annotation was first performed by matching experimental m/z, retention time and MS/MS spectra to an in-house library of analytical-grade standards. Remaining peaks were identified by matching experimental m/z and fragmentation spectra to publicly available databases including HMDB (http://www.hmdb.ca/), MoNA (http://mona.fiehnlab.ucdavis.edu/) and MassBank (http://www.massbank.jp/) using the R package ‘MetID’ (v0.2.0) (Shen et al., 2019). Briefly, metabolic feature tables from Progenesis QI were matched to fragmentation spectra with a m/z and a retention time window of ± 15 ppm and ± 30 s (HILIC) and ± 20 s (RPLC), respectively. When multiple MS/MS spectra match a single metabolic feature, all matched MS/MS spectra were used for the identification. Next, MS1 and MS2 pairs were searched against public databases and a similarity score was calculated using the forward dot–product algorithm which considers both fragments and intensities (Stein and Scott, 1994). Metabolites were reported if the similarity score was above 0.4. Spectra from metabolic features of interest important in random forest models (see below) were further investigated manually to confirm identification.","CAPILLARY_TEMPERATURE":"375C","CAPILLARY_VOLTAGE":"3.4kV","COLLISION_ENERGY":"25 & 35 NCE","COLLISION_GAS":"N2","DRY_GAS_TEMP":"310C","MS_RESULTS_FILE":"ST001491_AN002471_Results.txt UNITS:MS Counts Has m/z:Yes Has RT:Yes RT units:Minutes"}

}