{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001508","ANALYSIS_ID":"AN002499","VERSION":"1","CREATED_ON":"October 12, 2020, 12:13 pm"},

"PROJECT":{"PROJECT_TITLE":"Hepatic [U-13C]Lactate tracing and metabolomics in young and old WT and SIRT6 overexpressing mice","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression, but not in liver-specific SIRT6 overexpression. Here we preformed liver metabolomics in old liver-specific SIRT6 overexpression and control mice.","INSTITUTE":"Bar Ilan University","DEPARTMENT":"The Mina & Everard Goodman Faculty of Life Sciences","LABORATORY":"Laboratory of Prof. Haim Cohen","LAST_NAME":"Cohen","FIRST_NAME":"Chaim","ADDRESS":"Ramat Gan, Ramat Gan, Israel, 5290002, Israel","EMAIL":"Haim.Cohen@biu.ac.il","PHONE":"+97235318383","FUNDING_SOURCE":"Israel Science Foundation (621/13 and 777/16), I-Core foundation (1775/12) and (41/11) and Laura and Isaac Perlmutter Foundation","CONTRIBUTORS":"Asael Roichman, Ifat Abramovich, Eyal Gottlieb, Haim Yosef Cohen"},

"STUDY":{"STUDY_TITLE":"Liver metabolomics in old liver-specific SIRT6 overexpressing mice","STUDY_SUMMARY":"Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression, but not in liver-specific SIRT6 overexpression. Here we preformed liver metabolomics in old liver-specific SIRT6 overexpression and control mice.","INSTITUTE":"Bar Ilan University","DEPARTMENT":"The Mina & Everard Goodman Faculty of Life Sciences","LABORATORY":"Laboratory of Prof. Haim Cohen","LAST_NAME":"Cohen","FIRST_NAME":"Chaim","ADDRESS":"Bar Ilan University, Ramat Gan, Israel, 5290002","EMAIL":"Haim.Cohen@biu.ac.il","PHONE":"+97235318383","NUM_GROUPS":"4","TOTAL_SUBJECTS":"20"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6JOlaHsd","AGE_OR_AGE_RANGE":"23-25 months","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Envigo","ANIMAL_HOUSING":"Animal rooms were maintained at 21°C-24°C and 35%-75% relative humidity","ANIMAL_LIGHT_CYCLE":"12/12-h (7am to 7pm) dark-light cycle","ANIMAL_FEED":"Altromin 1324 (chow)","ANIMAL_WATER":"Ad lib"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"1",
"Sample ID":"01_Liver_OE",
"Factors":{"Genotype":"Liver specific SIRT6 overexpression"},
"Additional sample data":{"RAW_FILE_NAME":"01_Liver_OE"}
},
{
"Subject ID":"2",
"Sample ID":"02_Liver_KISS",
"Factors":{"Genotype":"Flox control"},
"Additional sample data":{"RAW_FILE_NAME":"02_Liver_KISS"}
},
{
"Subject ID":"3",
"Sample ID":"03_Liver_OE",
"Factors":{"Genotype":"Liver specific SIRT6 overexpression"},
"Additional sample data":{"RAW_FILE_NAME":"03_Liver_OE"}
},
{
"Subject ID":"4",
"Sample ID":"04_Liver_CRE",
"Factors":{"Genotype":"Albumin Cre control"},
"Additional sample data":{"RAW_FILE_NAME":"04_Liver_CRE"}
},
{
"Subject ID":"5",
"Sample ID":"05_Liver_WT",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"05_Liver_WT"}
},
{
"Subject ID":"6",
"Sample ID":"06_Liver_KISS",
"Factors":{"Genotype":"Flox control"},
"Additional sample data":{"RAW_FILE_NAME":"06_Liver_KISS"}
},
{
"Subject ID":"7",
"Sample ID":"07_Liver_CRE",
"Factors":{"Genotype":"Albumin Cre control"},
"Additional sample data":{"RAW_FILE_NAME":"07_Liver_CRE"}
},
{
"Subject ID":"8",
"Sample ID":"08_Liver_CRE",
"Factors":{"Genotype":"Albumin Cre control"},
"Additional sample data":{"RAW_FILE_NAME":"08_Liver_CRE"}
},
{
"Subject ID":"9",
"Sample ID":"09_Liver_WT",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"09_Liver_WT"}
},
{
"Subject ID":"10",
"Sample ID":"10_Liver_OE",
"Factors":{"Genotype":"Liver specific SIRT6 overexpression"},
"Additional sample data":{"RAW_FILE_NAME":"10_Liver_OE"}
},
{
"Subject ID":"11",
"Sample ID":"11_Liver_KISS",
"Factors":{"Genotype":"Flox control"},
"Additional sample data":{"RAW_FILE_NAME":"11_Liver_KISS"}
},
{
"Subject ID":"12",
"Sample ID":"12_Liver_KISS",
"Factors":{"Genotype":"Flox control"},
"Additional sample data":{"RAW_FILE_NAME":"12_Liver_KISS"}
},
{
"Subject ID":"13",
"Sample ID":"13_Liver_WT",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"13_Liver_WT"}
},
{
"Subject ID":"14",
"Sample ID":"14_Liver_KISS",
"Factors":{"Genotype":"Flox control"},
"Additional sample data":{"RAW_FILE_NAME":"14_Liver_KISS"}
},
{
"Subject ID":"15",
"Sample ID":"15_Liver_WT",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"15_Liver_WT"}
},
{
"Subject ID":"16",
"Sample ID":"16_Liver_CRE",
"Factors":{"Genotype":"Albumin Cre control"},
"Additional sample data":{"RAW_FILE_NAME":"16_Liver_CRE"}
},
{
"Subject ID":"17",
"Sample ID":"17_Liver_OE",
"Factors":{"Genotype":"Liver specific SIRT6 overexpression"},
"Additional sample data":{"RAW_FILE_NAME":"17_Liver_OE"}
},
{
"Subject ID":"18",
"Sample ID":"18_Liver_OE",
"Factors":{"Genotype":"Liver specific SIRT6 overexpression"},
"Additional sample data":{"RAW_FILE_NAME":"18_Liver_OE"}
},
{
"Subject ID":"19",
"Sample ID":"19_Liver_CRE",
"Factors":{"Genotype":"Albumin Cre control"},
"Additional sample data":{"RAW_FILE_NAME":"19_Liver_CRE"}
},
{
"Subject ID":"20",
"Sample ID":"20_Liver_WT",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"20_Liver_WT"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mice were euthanized by cervical dislocation, left liver lobe was collected as fast as possible (<30 sec) and immediately flash-frozen in liquid nitrogen. Tissues were stored at -80C until metabolite extraction.","SAMPLE_TYPE":"Liver","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"All mice were fasted for 6h (8AM-2PM). Tail blood was collected from all mice for less than 2 minutes, followed by mouse sacrifice.","ANIMAL_FASTING":"6h morning fast","ANIMAL_ENDP_EUTHANASIA":"Cervical dislocation"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Frozen liver pieces weighting ~30mg were transferred into soft tissue homogenizing CK 14 tubes containing 1.4 mm ceramic beads (Bertin corp.) prefilled with 1 ml of cold (-20°C) metabolites extraction solvent (Methanol: Acetonitrile:H2O, 50:30:20) and kept on ice. Samples were homogenized using precellys 24 tissue homogenizer (3X20sec at 6000 rpm, with a 30sec gap between each of the three cycles, Bertin Technologies) cooled to 2°C. Homogenized extracts were centrifuged in the Precellys tubes at 18,000g for 15 minutes at 4°C, supernatants were collected in microcentrifuge tubes and centrifuged again at 18,000g for 10 minutes at 4°C. The supernatants were transferred to glass HPLC vials and kept at -75 °C prior to LC-MS analysis.","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"SeQuant ZIC-HILIC (150 x 2.1mm, 5um)","FLOW_RATE":"0.2 ml/min","COLUMN_TEMPERATURE":"45","SOLVENT_A":"20 mM ammonium carbonate adjusted to pH 9.2 with 0.1% of 25% ammonium hydroxide","SOLVENT_B":"acetonitrile"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Prof. Eyal Gottlieb","OPERATOR_NAME":"Ifat Abramovich"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"polarity switching mode to enable both positive and negative ions was used"},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"Acetoacetate","01_Liver_OE":"127084.9983","02_Liver_KISS":"999443.0165","03_Liver_OE":"790517.8538","04_Liver_CRE":"966428.1878","05_Liver_WT":"1033130.001","06_Liver_KISS":"1095551.536","07_Liver_CRE":"1544269.469","08_Liver_CRE":"778113.5969","09_Liver_WT":"598420.7528","10_Liver_OE":"222553.0582","11_Liver_KISS":"744297.8193","12_Liver_KISS":"736593.3499","13_Liver_WT":"1020195.437","14_Liver_KISS":"448078.7548","15_Liver_WT":"926218.0015","16_Liver_CRE":"372857.2959","17_Liver_OE":"908632.7231","18_Liver_OE":"1780549.332","19_Liver_CRE":"638207.2021","20_Liver_WT":"629998.0201"},{"Metabolite":"Acetyl CoA","01_Liver_OE":"3136063.986","02_Liver_KISS":"5144632.104","03_Liver_OE":"8259400.965","04_Liver_CRE":"4804478.916","05_Liver_WT":"5832341.046","06_Liver_KISS":"5354130.482","07_Liver_CRE":"6596955.092","08_Liver_CRE":"4281381.318","09_Liver_WT":"5090962.285","10_Liver_OE":"4971963.593","11_Liver_KISS":"6254648.927","12_Liver_KISS":"5121737.919","13_Liver_WT":"5478406.382","14_Liver_KISS":"9706382.253","15_Liver_WT":"8370840.465","16_Liver_CRE":"5318609.185","17_Liver_OE":"7368794.456","18_Liver_OE":"6840295.482","19_Liver_CRE":"7097032.155","20_Liver_WT":"6088965.848"},{"Metabolite":"aKG","01_Liver_OE":"5953099.565","02_Liver_KISS":"10504805.42","03_Liver_OE":"12186579.92","04_Liver_CRE":"19589224.94","05_Liver_WT":"14427985.21","06_Liver_KISS":"16224614.48","07_Liver_CRE":"16571371.37","08_Liver_CRE":"10673567.41","09_Liver_WT":"12723022.45","10_Liver_OE":"12944186.63","11_Liver_KISS":"16332652.53","12_Liver_KISS":"9893577.402","13_Liver_WT":"16358531.22","14_Liver_KISS":"11845380.28","15_Liver_WT":"11428423.23","16_Liver_CRE":"14205399.64","17_Liver_OE":"11706920.51","18_Liver_OE":"11038294","19_Liver_CRE":"8227429.528","20_Liver_WT":"13507015.75"},{"Metabolite":"Arginine","01_Liver_OE":"1615353.1","02_Liver_KISS":"1257820.643","03_Liver_OE":"697882.9898","04_Liver_CRE":"894402.748","05_Liver_WT":"1099746.198","06_Liver_KISS":"696609.2792","07_Liver_CRE":"680926.9012","08_Liver_CRE":"1634123.698","09_Liver_WT":"704249.3457","10_Liver_OE":"786678.8479","11_Liver_KISS":"689345.2992","12_Liver_KISS":"1400634.394","13_Liver_WT":"646634.5882","14_Liver_KISS":"624637.8868","15_Liver_WT":"509998.773","16_Liver_CRE":"725835.1903","17_Liver_OE":"517275.7161","18_Liver_OE":"579202.1715","19_Liver_CRE":"751040.8395","20_Liver_WT":"932120.3408"},{"Metabolite":"Asparagine","01_Liver_OE":"18569933.46","02_Liver_KISS":"21966005.94","03_Liver_OE":"21216861.6","04_Liver_CRE":"19985660.62","05_Liver_WT":"20521877.4","06_Liver_KISS":"20287818.71","07_Liver_CRE":"19215814.08","08_Liver_CRE":"20694044.5","09_Liver_WT":"18857844.29","10_Liver_OE":"29132413.52","11_Liver_KISS":"18903980.07","12_Liver_KISS":"25430558.89","13_Liver_WT":"19312608.65","14_Liver_KISS":"19067200.78","15_Liver_WT":"19378729.41","16_Liver_CRE":"20864970.6","17_Liver_OE":"21420332.35","18_Liver_OE":"28064804.56","19_Liver_CRE":"20687889.38","20_Liver_WT":"19628554.46"},{"Metabolite":"Aspartate","01_Liver_OE":"96252060.6","02_Liver_KISS":"115712671.2","03_Liver_OE":"55945394.35","04_Liver_CRE":"97369995.69","05_Liver_WT":"48821572.12","06_Liver_KISS":"54784480.31","07_Liver_CRE":"56013456.81","08_Liver_CRE":"56157530.07","09_Liver_WT":"151487238.5","10_Liver_OE":"117727876.8","11_Liver_KISS":"99692488.1","12_Liver_KISS":"117383469.4","13_Liver_WT":"66739496.31","14_Liver_KISS":"101346626.2","15_Liver_WT":"88980603.84","16_Liver_CRE":"107295010.5","17_Liver_OE":"69293984.87","18_Liver_OE":"87702680.14","19_Liver_CRE":"73099426.22","20_Liver_WT":"112396204.2"},{"Metabolite":"cis-aconitate","01_Liver_OE":"1927495.465","02_Liver_KISS":"2932669.196","03_Liver_OE":"2498581.543","04_Liver_CRE":"2847543.031","05_Liver_WT":"3268500.679","06_Liver_KISS":"2221548.463","07_Liver_CRE":"2749976.8","08_Liver_CRE":"3469843.538","09_Liver_WT":"2047188.716","10_Liver_OE":"2368110.233","11_Liver_KISS":"2769199.929","12_Liver_KISS":"1770021.474","13_Liver_WT":"2224624.96","14_Liver_KISS":"1887794.638","15_Liver_WT":"2048842.867","16_Liver_CRE":"2821394.845","17_Liver_OE":"2360362.181","18_Liver_OE":"3068232.578","19_Liver_CRE":"1261037.993","20_Liver_WT":"1838566.312"},{"Metabolite":"Citrate","01_Liver_OE":"68791194.15","02_Liver_KISS":"97323618.78","03_Liver_OE":"90516770.97","04_Liver_CRE":"108626234.5","05_Liver_WT":"110119094.1","06_Liver_KISS":"83971012.84","07_Liver_CRE":"105570532.8","08_Liver_CRE":"87334274.83","09_Liver_WT":"85447609.45","10_Liver_OE":"89131701.19","11_Liver_KISS":"95434245.06","12_Liver_KISS":"64956210.93","13_Liver_WT":"86440975.48","14_Liver_KISS":"81540239.6","15_Liver_WT":"82900010.17","16_Liver_CRE":"99815017.15","17_Liver_OE":"94665657.51","18_Liver_OE":"106436105.6","19_Liver_CRE":"60940751.27","20_Liver_WT":"80637463.59"},{"Metabolite":"Cysteine","01_Liver_OE":"27547.42073","02_Liver_KISS":"5876.812933","03_Liver_OE":"0","04_Liver_CRE":"5958.375454","05_Liver_WT":"8228.444015","06_Liver_KISS":"13644.93549","07_Liver_CRE":"12949.24819","08_Liver_CRE":"7221.566738","09_Liver_WT":"6006.218893","10_Liver_OE":"12038.93103","11_Liver_KISS":"5963.895244","12_Liver_KISS":"12974.67255","13_Liver_WT":"0","14_Liver_KISS":"0","15_Liver_WT":"5117.979916","16_Liver_CRE":"0","17_Liver_OE":"6270.911411","18_Liver_OE":"6114.079005","19_Liver_CRE":"0","20_Liver_WT":"0"},{"Metabolite":"Cystine","01_Liver_OE":"677484.358","02_Liver_KISS":"282114.7135","03_Liver_OE":"121602.7692","04_Liver_CRE":"24041.31086","05_Liver_WT":"233713.2709","06_Liver_KISS":"178985.1157","07_Liver_CRE":"149225.2787","08_Liver_CRE":"290393.0943","09_Liver_WT":"36264.84814","10_Liver_OE":"321462.3923","11_Liver_KISS":"126072.0436","12_Liver_KISS":"370520.7397","13_Liver_WT":"178791.8653","14_Liver_KISS":"66076.59719","15_Liver_WT":"251415.6784","16_Liver_CRE":"143099.4733","17_Liver_OE":"70089.30595","18_Liver_OE":"150823.9924","19_Liver_CRE":"92094.41073","20_Liver_WT":"12731.21529"},{"Metabolite":"dihydroxyacetone phosphate 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}

}