{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001704","ANALYSIS_ID":"AN002776","VERSION":"1","CREATED_ON":"February 17, 2021, 1:40 pm"},

"PROJECT":{"PROJECT_TITLE":"Sclerostin antibody increases trabecular bone and bone mechanical properties by increasing osteoblast activity damaged by whole-body irradiation in mice","PROJECT_TYPE":"Proteomics and Lipidomics basic research","PROJECT_SUMMARY":"A mouse model study to test the hypothesis that treatment with anti-sclerostin antibody (Scl-Ab) would attenuate the adverse effects of irradiation by increasing bone volume and decreasing BM adipose tissue (BMAT), resulting in better quality bone.","INSTITUTE":"Mainehealth","LAST_NAME":"Vary","FIRST_NAME":"Calvin","ADDRESS":"81 Research Drive, Scarborough, ME, USA 04074","EMAIL":"varyc@mmc.org","PHONE":"2073968148","FUNDING_SOURCE":"NIH"},

"STUDY":{"STUDY_TITLE":"Sclerostin antibody increases trabecular bone and bone mechanical properties by increasing osteoblast activity damaged by whole-body irradiation in mice","STUDY_TYPE":"Basic research","STUDY_SUMMARY":"Irradiation therapy causes bone deterioration and increased risk for skeletal-related events. Irradiation interferes with trabecular architecture through increased osteoclastic activity, decreased osteoblastic activity, and increased adipocyte expansion in the bone marrow (BM), which further compounds bone-related disease. Neutralizing antibodies to sclerostin (Scl-Ab) increase bone mass and strength by increasing bone formation and reducing bone resorption. We hypothesized that treatment with Scl-Ab would attenuate the adverse effects of irradiation by increasing bone volume and decreasing BM adipose tissue (BMAT), resulting in better quality bone. In this study, 12-week-old female C57BL/6J mice were exposed to 6 Gy whole-body irradiation or were non-irradiated, then administered Scl-Ab (25 mg/kg) or vehicle weekly for 5 weeks. Femoral µCT analysis confirmed that the overall effect of IR significantly decreased trabecular bone volume/total volume (Tb.BV/TV) (2-way ANOVA, p<0.0001) with a -43.8% loss in Tb.BV/TV in the IR control group. Scl-Ab independently increased Tb.BV/TV by 3.07-fold in non-irradiated and 3.6-fold in irradiated mice (2-way ANOVA, p<0.0001). Irradiation did not affect cortical parameters, although Scl-Ab increased cortical thickness and area significantly in both irradiated and non-irradiated mice (2-way ANOVA, p<0.0001). Femoral mechanical testing confirmed Scl-Ab significantly increased bending rigidity and ultimate moment independently of irradiation (2-way ANOVA, p<0.0001). Static and dynamic histomorphometry of the femoral metaphysis revealed osteoblast vigor, not number, was significantly increased in the irradiated mice treated with Scl-Ab. Systemic alterations were assessed through serum lipidomic analysis, which showed that Scl-Ab normalized lipid profiles in the irradiated group. This data supports the theory of sclerostin as a novel contributor to the regulation of osteoblast activity after irradiation. Overall, our data support the hypothesis that Scl-Ab ameliorates the deleterious effects of whole-body irradiation on bone and adipose tissue in a mouse model. Our findings suggest that future research into localized and systemic therapies after irradiation exposure is warranted.","INSTITUTE":"Mainehealth","LAST_NAME":"Vary","FIRST_NAME":"Calvin","ADDRESS":"81 Research Drive","EMAIL":"varyc@mmc.org","PHONE":"2073968148"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","AGE_OR_AGE_RANGE":"12-17 weeks","GENDER":"Female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C100_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C100_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C100_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C100_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C101_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C101_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C101_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C101_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C107_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C107_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C107_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C107_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C133_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C133_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C133_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C133_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C135_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C135_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C135_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C135_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C136_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C136_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_C136_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_C136_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA121_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA121_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA121_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA121_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA123_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA123_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA123_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA123_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA127_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA127_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA127_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA127_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA152_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA152_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA152_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA152_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA153_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA153_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA153_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA153_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA158_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA158_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D35-IR_SA158_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D35-IR_SA158_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C100_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C100_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C100_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C100_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C101_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C101_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C101_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C101_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C107_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C107_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C107_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C107_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C133_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C133_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C133_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C133_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C135_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C135_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C135_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C135_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C136_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C136_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_C136_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_C136_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA121_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA121_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA121_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA121_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA123_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA123_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA123_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA123_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA127_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA127_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA127_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA127_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA152_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA152_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA152_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA152_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA153_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA153_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA153_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA153_2"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA158_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA158_1"}
},
{
"Subject ID":"-",
"Sample ID":"posMSMSALL-SC-D3-IR_SA158_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"posMSMSALL-SC-D3-IR_SA158_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C100_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C100_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C100_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C100_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C101_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C101_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C101_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C101_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C107_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C107_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C107_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C107_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C133_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C133_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C133_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C133_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C135_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C135_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C135_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C135_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C136_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C136_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_C136_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_C136_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA121_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA121_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA121_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA121_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA123_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA123_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA123_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA123_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA127_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA127_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA127_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA127_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA152_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA152_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA152_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA152_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA153_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA153_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA153_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA153_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA158_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA158_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D35-IR_SA158_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D35-IR_SA158_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C100_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C100_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C100_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C100_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C101_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C101_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C101_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C101_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C107_1",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C107_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C107_2",
"Factors":{"Treatment":"IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C107_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C133_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C133_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C133_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C133_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C135_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C135_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C135_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C135_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C136_1",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C136_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_C136_2",
"Factors":{"Treatment":"IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_C136_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA121_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA121_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA121_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA121_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA123_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA123_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA123_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA123_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA127_1",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA127_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA127_2",
"Factors":{"Treatment":"Non-IR Control"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA127_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA152_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA152_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA152_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA152_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA153_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA153_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA153_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA153_2"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA158_1",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA158_1"}
},
{
"Subject ID":"-",
"Sample ID":"negMSMSALL-SC-D3-IR_SA158_2",
"Factors":{"Treatment":"Non-IR + Scl-Ab"},
"Additional sample data":{"Strain":"C57BL/6J","RAW_FILE_NAME":"negMSMSALL-SC-D3-IR_SA158_2"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Lipidomic analyses were performed on mouse serum (n=3) collected through submandibular and terminal blood collections. Blood samples were extracted (10% of total body weight) and allowed to clot for 60 minutes at room temperature at which point the samples were centrifuged for 15 minutes at 12,000 RCF. The upper phase containing serum was transferred to a new, sterile microcentrifuge tube and frozen at -80°C.","SAMPLE_TYPE":"Blood (serum)","COLLECTION_METHOD":"submandibular and terminal blood collection","COLLECTION_FREQUENCY":"Day 3 and Day 35","STORAGE_CONDITIONS":"-80℃","COLLECTION_VIALS":"sterile microcentrifuge tube","STORAGE_VIALS":"sterile microcentrifuge tube"},

"TREATMENT":{"TREATMENT_SUMMARY":"Forty female C57BL/6J mice (Jackson Laboratory, Bar Harbor, ME, USA), 12 weeks of age, were randomly split between two cohorts; Irradiated (IR) and Non-Irradiated (Non-IR) (n=20) and were used for all experiments. Two sub-lethal doses of cesium-137 (Cs-137) whole-body irradiation (2x 3 Gy; total of 6 Gy) with an exposure time of 3.72 minutes per mouse (total of 7.44 minutes) were administered 4 hours apart to the IR mice (n=20) on Day 0. Within each cohort, mice were sub-divided into treatment groups; Vehicle and Scl-Ab VI (25 mg/kg) (supplied by Amgen Inc/UCB Pharma) (n=10/per treatment). All mice were administered either a sterile phosphate buffered saline (PBS) vehicle control or Scl-Ab (25 mg/kg), once weekly via subcutaneous injections for a duration of 5 weeks, starting on Day 0. No adverse effects were observed after the administration of Scl-Ab. All experimental studies and procedures involving mice were performed in accordance with protocols approved by the governing Institutional Animal Care and Use Committee (IACUC). Additional Study Design and Drug Treatment information can be found in the Supplemental Material.","TREATMENT":"Neutralizing antibodies to sclerostin (Scl-Ab)","TREATMENT_ROUTE":"subcutaneous injections","TREATMENT_DOSE":"25 mg/kg","TREATMENT_DOSEDURATION":"once weekly for 5 weeks","TREATMENT_VEHICLE":"sterile phosphate buffered saline (PBS) vehicle control","TREATMENT_COMPOUND":"Scl-Ab VI (supplied by Amgen Inc/UCB Pharma)"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Three 12 µL serum aliquots per treatment (representative of individual mice) were provided to the MMCRI Proteomics and Lipidomics Core Facility for analysis. The individual samples were sub-divided into technical replicates, which were then averaged together. Lipid extracts were dissolved in methanol/dichloromethane for mass spectrometry (MS) analysis. Lipidomic analyses were completed via 5600 TripleTOF mass spectrometer (Sciex, Framingham, MA) and downstream analyses, including t-tests and principal component analyses (PCAs), were completed utilizing MarkerView Software (Sciex). PCAs were performed with no weighting, Pareto scaling, and supervised data analysis. Lipids were analyzed using a global, bias-free lipid profiling acquisition technique (MS/MSALL).","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Direct infusion","CHROMATOGRAPHY_TYPE":"None (Direct infusion)","INSTRUMENT_NAME":"Sciex TripleTOF 5600","COLUMN_NAME":"none"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Proteomics and Lipidomics Core","OPERATOR_NAME":"Calvin Vary"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5600 TripleTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Direct infusion positive and negative mode MSMSALL workflow Sciex-LipidView; Sciex-MarkerView","MS_RESULTS_FILE":"ST001704_AN002776_Results.txt UNITS:m/z Has m/z:No Has RT:No RT units:No RT data"}

}