{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001723","ANALYSIS_ID":"AN002807","VERSION":"1","CREATED_ON":"March 17, 2021, 9:36 am"},

"PROJECT":{"PROJECT_TITLE":"The effects of birth weight and breeding value for protein deposition on the plasma metabolome in growing pigs","PROJECT_SUMMARY":"An experiment was conducted with growing pigs, to determine the effects of birth weight (BiW) and estimated breeding value for protein deposition (EBV) on the metabolomic profile in plasma samples collected under different dietary regimens: protein adequate (A) or protein restricted (R, 70% of A).","INSTITUTE":"Aarhus University","DEPARTMENT":"Animal Science","LABORATORY":"Metabolomics LC-MS platform Aarhus University Foulum","LAST_NAME":"Hedemann","FIRST_NAME":"Mette","ADDRESS":"Blichers Alle 20, Tjele, -, 8830, Denmark","EMAIL":"Mette.Hedemann@anis.au.dk","PHONE":"51448783","FUNDING_SOURCE":"European Union's H2020 Program","PROJECT_COMMENTS":"Feed-a-Gene, Grant agreement no 633531","PUBLICATIONS":"The effects of birth weight and breeding value for protein deposition on nitrogen efficiency in growing pigs","CONTRIBUTORS":"C.M.C. van der Peet-Schwering, L.M.G. Verschuren, R. Bergsma, M.S. Hedemann, G.P. Binnendijk, A.J.M. Jansman"},

"STUDY":{"STUDY_TITLE":"The effects of birth weight and breeding value for protein deposition on the plasma metabolome in growing pigs (part-II)","STUDY_SUMMARY":"An experiment was conducted with growing pigs, to determine the effects of birth weight (BiW) and estimated breeding value for protein deposition (EBV) on the metabolomic profile in plasma samples collected under different dietary regimens: protein adequate (A) or protein restricted (R, 70% of A).","INSTITUTE":"Aarhus University","DEPARTMENT":"Animal Science","LABORATORY":"Metabolomics LC-MS platform Aarhus University Foulum","LAST_NAME":"Hedemann","FIRST_NAME":"Mette","ADDRESS":"Blichers Alle 20, Tjele, -, 8830, Denmark","EMAIL":"Mette.Hedemann@anis.au.dk","PHONE":"51448783","TOTAL_SUBJECTS":"40","NUM_MALES":"40"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Sus scrofa","TAXONOMY_ID":"9823","AGE_OR_AGE_RANGE":"0-125 days","WEIGHT_OR_WEIGHT_RANGE":"1-80 kg","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Swine Innovation Centre Sterksel, the Netherlands","ANIMAL_HOUSING":"Metabolism cages","ANIMAL_LIGHT_CYCLE":"12h light / 12h darknes","ANIMAL_FEED":"Experimental diets (see publication)","ANIMAL_WATER":"Ad libitum","ANIMAL_INCLUSION_CRITERIA":"Birth weight"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"QC",
"Sample ID":"QC-1_pos_1a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-1_pos_1a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-2_pos_1a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-2_pos_1a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-3_pos_1a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-3_pos_1a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-4_pos_1a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-4_pos_1a.mzXML"}
},
{
"Subject ID":"MM9001",
"Sample ID":"Plasma_pos_21",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"10.325","Wght_birth":"2.05","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_21.mzXML"}
},
{
"Subject ID":"MM9189",
"Sample ID":"Plasma_pos_22",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"1.003","Wght_birth":"1.70","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_22.mzXML"}
},
{
"Subject ID":"MM9003",
"Sample ID":"Plasma_pos_23",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"9.259","Wght_birth":"1.93","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_23.mzXML"}
},
{
"Subject ID":"MM9294",
"Sample ID":"Plasma_pos_24",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-4.591","Wght_birth":"1.89","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_24.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-1_pos_2a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-1_pos_2a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-2_pos_2a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-2_pos_2a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-3_pos_2a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-3_pos_2a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-4_pos_2a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-4_pos_2a.mzXML"}
},
{
"Subject ID":"MM9215",
"Sample ID":"Plasma_pos_25",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-4.771","Wght_birth":"0.93","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_25.mzXML"}
},
{
"Subject ID":"MM9096",
"Sample ID":"Plasma_pos_26",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"5.035","Wght_birth":"1.04","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_26.mzXML"}
},
{
"Subject ID":"MM9104",
"Sample ID":"Plasma_pos_27",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"7.067","Wght_birth":"1.15","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_27.mzXML"}
},
{
"Subject ID":"MM9300",
"Sample ID":"Plasma_pos_28",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.142","Wght_birth":"1.13","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_28.mzXML"}
},
{
"Subject ID":"MM9150",
"Sample ID":"Plasma_pos_29",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-4.872","Wght_birth":"1.95","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_29.mzXML"}
},
{
"Subject ID":"MM9062",
"Sample ID":"Plasma_pos_30",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"13.209","Wght_birth":"0.95","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_30.mzXML"}
},
{
"Subject ID":"MM9219",
"Sample ID":"Plasma_pos_31",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"0.031","Wght_birth":"1.61","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_31.mzXML"}
},
{
"Subject ID":"MM9221",
"Sample ID":"Plasma_pos_32",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-3.470","Wght_birth":"1.10","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_32.mzXML"}
},
{
"Subject ID":"MM9303",
"Sample ID":"Plasma_pos_33",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"9.738","Wght_birth":"1.72","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_33.mzXML"}
},
{
"Subject ID":"MM9154",
"Sample ID":"Plasma_pos_34",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-0.558","Wght_birth":"1.84","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_34.mzXML"}
},
{
"Subject ID":"MM9325",
"Sample ID":"Plasma_pos_35",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.882","Wght_birth":"1.16","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_35.mzXML"}
},
{
"Subject ID":"MM9188",
"Sample ID":"Plasma_pos_36",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"8.689","Wght_birth":"1.77","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_36.mzXML"}
},
{
"Subject ID":"MM9310",
"Sample ID":"Plasma_pos_37",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"16.944","Wght_birth":"0.78","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_37.mzXML"}
},
{
"Subject ID":"MM9006",
"Sample ID":"Plasma_pos_38",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"13.239","Wght_birth":"1.77","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_38.mzXML"}
},
{
"Subject ID":"MM9149",
"Sample ID":"Plasma_pos_39",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-3.794","Wght_birth":"1.89","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_39.mzXML"}
},
{
"Subject ID":"MM9004",
"Sample ID":"Plasma_pos_40",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"18.784","Wght_birth":"1.87","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_40.mzXML"}
},
{
"Subject ID":"MM9001",
"Sample ID":"Plasma_pos_41",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"10.325","Wght_birth":"2.05","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_41.mzXML"}
},
{
"Subject ID":"MM9189",
"Sample ID":"Plasma_pos_42",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"1.003","Wght_birth":"1.70","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_42.mzXML"}
},
{
"Subject ID":"MM9003",
"Sample ID":"Plasma_pos_43",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"9.259","Wght_birth":"1.93","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_43.mzXML"}
},
{
"Subject ID":"MM9294",
"Sample ID":"Plasma_pos_44",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-4.591","Wght_birth":"1.89","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_44.mzXML"}
},
{
"Subject ID":"MM9215",
"Sample ID":"Plasma_pos_45",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-4.771","Wght_birth":"0.93","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_45.mzXML"}
},
{
"Subject ID":"MM9096",
"Sample ID":"Plasma_pos_46",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"5.035","Wght_birth":"1.04","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_46.mzXML"}
},
{
"Subject ID":"MM9104",
"Sample ID":"Plasma_pos_47",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"7.067","Wght_birth":"1.15","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_47.mzXML"}
},
{
"Subject ID":"MM9300",
"Sample ID":"Plasma_pos_48",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.142","Wght_birth":"1.13","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_48.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-1_pos_3a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-1_pos_3a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-2_pos_3a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-2_pos_3a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-3_pos_3a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-3_pos_3a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-4_pos_3a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-4_pos_3a.mzXML"}
},
{
"Subject ID":"MM9150",
"Sample ID":"Plasma_pos_49",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-4.872","Wght_birth":"1.95","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_49.mzXML"}
},
{
"Subject ID":"MM9062",
"Sample ID":"Plasma_pos_50",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"13.209","Wght_birth":"0.95","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_50.mzXML"}
},
{
"Subject ID":"MM9219",
"Sample ID":"Plasma_pos_51",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"0.031","Wght_birth":"1.61","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_51.mzXML"}
},
{
"Subject ID":"MM9221",
"Sample ID":"Plasma_pos_52",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-3.470","Wght_birth":"1.10","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_52.mzXML"}
},
{
"Subject ID":"MM9303",
"Sample ID":"Plasma_pos_53",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"9.738","Wght_birth":"1.72","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_53.mzXML"}
},
{
"Subject ID":"MM9154",
"Sample ID":"Plasma_pos_54",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-0.558","Wght_birth":"1.84","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_54.mzXML"}
},
{
"Subject ID":"MM9325",
"Sample ID":"Plasma_pos_55",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.882","Wght_birth":"1.16","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_55.mzXML"}
},
{
"Subject ID":"MM9188",
"Sample ID":"Plasma_pos_56",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"8.689","Wght_birth":"1.77","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_56.mzXML"}
},
{
"Subject ID":"MM9310",
"Sample ID":"Plasma_pos_57",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"16.944","Wght_birth":"0.78","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_57.mzXML"}
},
{
"Subject ID":"MM9006",
"Sample ID":"Plasma_pos_58",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"13.239","Wght_birth":"1.77","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_58.mzXML"}
},
{
"Subject ID":"MM9149",
"Sample ID":"Plasma_pos_59",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-3.794","Wght_birth":"1.89","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_59.mzXML"}
},
{
"Subject ID":"MM9004",
"Sample ID":"Plasma_pos_60",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"18.784","Wght_birth":"1.87","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_60.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-1_pos_4a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-1_pos_4a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-2_pos_4a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-2_pos_4a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-3_pos_4a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-3_pos_4a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-4_pos_4a",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-4_pos_4a.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-1_pos_1",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-1_pos_1.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-2_pos_1",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-2_pos_1.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-3_pos_1",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-3_pos_1.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-4_pos_1",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-4_pos_1.mzXML"}
},
{
"Subject ID":"MM9309",
"Sample ID":"Plasma_pos_81",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"14.658","Wght_birth":"1.16","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_81.mzXML"}
},
{
"Subject ID":"MM9034",
"Sample ID":"Plasma_pos_82",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.057","Wght_birth":"1.16","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_82.mzXML"}
},
{
"Subject ID":"MM9171",
"Sample ID":"Plasma_pos_83",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-2.490","Wght_birth":"1.02","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_83.mzXML"}
},
{
"Subject ID":"MM9125",
"Sample ID":"Plasma_pos_84",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"10.481","Wght_birth":"0.84","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_84.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-1_pos_2",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-1_pos_2.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-2_pos_2",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-2_pos_2.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-3_pos_2",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-3_pos_2.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-4_pos_2",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-4_pos_2.mzXML"}
},
{
"Subject ID":"MM9304",
"Sample ID":"Plasma_pos_85",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"14.534","Wght_birth":"1.62","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_85.mzXML"}
},
{
"Subject ID":"MM9407",
"Sample ID":"Plasma_pos_86",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"8.842","Wght_birth":"1.01","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_86.mzXML"}
},
{
"Subject ID":"MM9315",
"Sample ID":"Plasma_pos_87",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.820","Wght_birth":"1.14","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_87.mzXML"}
},
{
"Subject ID":"MM9410",
"Sample ID":"Plasma_pos_88",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"14.929","Wght_birth":"1.16","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_88.mzXML"}
},
{
"Subject ID":"MM9305",
"Sample ID":"Plasma_pos_89",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"12.996","Wght_birth":"1.65","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_89.mzXML"}
},
{
"Subject ID":"MM9247",
"Sample ID":"Plasma_pos_90",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-12.409","Wght_birth":"1.09","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_90.mzXML"}
},
{
"Subject ID":"MM9152",
"Sample ID":"Plasma_pos_91",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-2.114","Wght_birth":"1.92","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_91.mzXML"}
},
{
"Subject ID":"MM9296",
"Sample ID":"Plasma_pos_92",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"0.786","Wght_birth":"1.86","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_92.mzXML"}
},
{
"Subject ID":"MM9295",
"Sample ID":"Plasma_pos_93",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"-3.789","Wght_birth":"1.72","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_93.mzXML"}
},
{
"Subject ID":"MM9266",
"Sample ID":"Plasma_pos_94",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"7.079","Wght_birth":"1.62","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_94.mzXML"}
},
{
"Subject ID":"MM9187",
"Sample ID":"Plasma_pos_95",
"Factors":{"Diet":"restricted","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"7.204","Wght_birth":"1.95","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_95.mzXML"}
},
{
"Subject ID":"MM9031",
"Sample ID":"Plasma_pos_96",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"8.422","Wght_birth":"1.07","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_96.mzXML"}
},
{
"Subject ID":"MM9117",
"Sample ID":"Plasma_pos_97",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-2.702","Wght_birth":"0.94","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_97.mzXML"}
},
{
"Subject ID":"MM9121",
"Sample ID":"Plasma_pos_98",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-1.224","Wght_birth":"1.01","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_98.mzXML"}
},
{
"Subject ID":"MM9177",
"Sample ID":"Plasma_pos_99",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"5.137","Wght_birth":"1.04","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_99.mzXML"}
},
{
"Subject ID":"MM9309",
"Sample ID":"Plasma_pos_100",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"14.658","Wght_birth":"1.16","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_100.mzXML"}
},
{
"Subject ID":"MM9034",
"Sample ID":"Plasma_pos_101",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.057","Wght_birth":"1.16","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_101.mzXML"}
},
{
"Subject ID":"MM9171",
"Sample ID":"Plasma_pos_102",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"-2.490","Wght_birth":"1.02","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_102.mzXML"}
},
{
"Subject ID":"MM9125",
"Sample ID":"Plasma_pos_103",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"10.481","Wght_birth":"0.84","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_103.mzXML"}
},
{
"Subject ID":"MM9304",
"Sample ID":"Plasma_pos_104",
"Factors":{"Diet":"adequate","Wght_birth_cat":"high"},
"Additional sample data":{"Breedvalue_animal":"14.534","Wght_birth":"1.62","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_104.mzXML"}
},
{
"Subject ID":"MM9407",
"Sample ID":"Plasma_pos_105",
"Factors":{"Diet":"adequate","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"8.842","Wght_birth":"1.01","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_105.mzXML"}
},
{
"Subject ID":"MM9315",
"Sample ID":"Plasma_pos_107",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"0.820","Wght_birth":"1.14","Breedvalue_cat":"low","RAW_FILE_NAME":"Plasma_pos_107.mzXML"}
},
{
"Subject ID":"MM9410",
"Sample ID":"Plasma_pos_108",
"Factors":{"Diet":"restricted","Wght_birth_cat":"low"},
"Additional sample data":{"Breedvalue_animal":"14.929","Wght_birth":"1.16","Breedvalue_cat":"high","RAW_FILE_NAME":"Plasma_pos_108.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-1_pos_3",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-1_pos_3.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-2_pos_3",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-2_pos_3.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-3_pos_3",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-3_pos_3.mzXML"}
},
{
"Subject ID":"QC",
"Sample ID":"QC-4_pos_3",
"Factors":{"Diet":"QC","Wght_birth_cat":"QC"},
"Additional sample data":{"Breedvalue_animal":"QC","Wght_birth":"QC","Breedvalue_cat":"QC","RAW_FILE_NAME":"QC-4_pos_3.mzXML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"At the end of each feeding regime, blood samples were collected from the jugular vein. Per sampling moment, two 9-mL plasma tubes (Vacuette, Greiner Bio-One, Kremsmünster, Austria) per pig were filled and allowed to clot for 1 h at room temperature. Plasma was collected after centrifugation for 15 min at 2,000 ∙ g and was stored at -80°C pending analyses.","SAMPLE_TYPE":"Blood (plasma)","COLLECTION_METHOD":"Jugular vein puncture","COLLECTION_FREQUENCY":"At the end of feeding period","VOLUMEORAMOUNT_COLLECTED":"2 x 9 ml blood","STORAGE_CONDITIONS":"-80℃","COLLECTION_VIALS":"Vacuette"},

"TREATMENT":{"TREATMENT_SUMMARY":"At an age of 14 weeks, 10 LBW-LEBV (BiW: 1.07 + 0.09 kg; EBV: -2.52 + 3.97 g/d, compared to an average crossbred pig with a protein deposition of 165 g/d), 10 LBW-HEBV (BiW: 1.02 + 0.13 kg; EBV: 10.47 + 4.26 g/d), 10 HBW-LEBV (BiW: 1.80 + 0.13 kg; EBV: - 2.15 + 2.28 g/d), and 10 HBW-HEBV (BiW: 1.80 + 0.15 kg; EBV: 11.18 + 3.68 g/d), male growing pigs (Synthetic boar x (Dutch Landrace x Large White)) were allotted to the experiment. The pigs were individually housed in metabolism cages (1.80 x 0.80 m) at a room temperature of 22oC. They were subjected to N- balance measurements in two sequential periods of 5 d using a restricted feeding regime. After a 6-d adaptation period to the metabolism cages, pigs were adapted for 5 days to the experimental diets before the start of the first 5-d balance period. Pigs were assigned to a protein adequate (A) or protein restricted (R, 70% of A) regime in a change-over design."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Plasma samples (150 µL) were pipetted into a 96-well plate and 450 µL acetonitrile containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL, was added per well. The samples were mixed immediately and placed at 4°C for 10 min for protein precipitation. After centrifugation (3700 rpm for 25 min at 4°C), the supernatant was transferred to a filter plate fixed on top of a 96-well collection plate in a manifold with a pressure gauge. Vacuum was then applied to the filter plate, and the solvent containing plasma components was collected into the 96-well collection plate. The collection plate was placed in a vacuum centrifuge and the samples were evaporated to dryness (ca. 2.5 h, 805 × g and 30°C). A volume of 150 µL of water/acetonitrile/formic acid (95/5/0.1%) was added to the dried extracts in the collection plate. The dried collection plate was then sealed with a piece of aluminum laminate (#186002789, Waters), centrifuged (3700 rpm for 25 min at 4°C), and kept in the auto-sampler at 10°C for the UPLC-QTOF/MS analysis. The injection volume was 3 µl.","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Chromatographic separation was performed on a Dionex UltiMate 3000RSL Binary UHPLC System (Thermo Scientific Dionex, Sunnyvale, CA) equipped with a HSS T3 C18 UHPLC column, 1.8 µm, 100x2.1 mm (Waters Corporation, Milford, MA). The column was maintained at 30°C. Samples were kept in the autosampler at 10°C, and the injection volume for both sample types was 3 µl. The mobile phases were 0.1% formic acid in Milli-Q water (A) and 0.1% formic acid in acetonitrile (B). The gradient program for the plasma samples was as follows: 0-12 min, linear gradient from 5-100% B; 12-13 min, 100% B and return to initial conditions in 0.2 min. In all cases, the column was re-equilibrated at 5% B for 2 min at the beginning of each run. The flow rate was 0.4 ml/min","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000 RS","COLUMN_NAME":"Waters Acquity BEH HSS T3 (100 x 2.1mm, 1.8um)","FLOW_RATE":"0.4 ml/min","COLUMN_TEMPERATURE":"30","SOLVENT_A":"0.1% formic acid in water","SOLVENT_B":"0.1% formic acid in acetonitrile"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Bruker Impact HD","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"The eluent was introduced into the mass spectrometer by electrospray ionization, with capillary set in the positive and negative mode to 4500 and 3600 V, respectively. End-plate offset voltage was set to 500 V. Nitrogen was used as both nebulizer and drying gas with a gas pressure of 1.8 bar. Drying gas temperature and flow were 200 °C and 8.0 L/min, respectively. Spectra were acquired over the scan range of 50−1000 m/z. A solution of lithium formate clusters (5 mM) (water/isopropanol/formic acid in a 50:50:0.2 v/v/v ratio) was injected prior to each chromatographic run as an external calibrant.","MS_RESULTS_FILE":"ST001723_AN002807_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes"}

}