{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001736","ANALYSIS_ID":"AN002826","VERSION":"1","CREATED_ON":"March 30, 2021, 7:26 am"},

"PROJECT":{"PROJECT_TITLE":"The COVIDome Explorer Researcher Portal","PROJECT_SUMMARY":"COVID-19 pathology involves dysregulation of diverse molecular, cellular, and physiological processes. In order to expedite integrated and collaborative COVID-19 research, we completed multi-omics analysis of hospitalized COVID-19 patients including matched analysis of the whole blood transcriptome, plasma proteomics with two complementary platforms, cytokine profiling, plasma and red blood cell metabolomics, deep immune cell phenotyping by mass cytometry, and clinical data annotation. We refer to this multidimensional dataset as the COVIDome. We then created the COVIDome Explorer, an online researcher portal where the data can be analyzed and visualized in real time. We illustrate here the use of the COVIDome dataset through a multi-omics analysis of biosignatures associated with C-reactive protein (CRP), an established marker of poor prognosis in COVID-19, revealing associations between CRP levels and damage-associated molecular patterns, depletion of protective serpins, and mitochondrial metabolism dysregulation. We expect that the COVIDome Explorer will rapidly accelerate data sharing, hypothesis testing, and discoveries worldwide.","INSTITUTE":"University of Colorado Anschutz Medical Campus","LAST_NAME":"Haines","FIRST_NAME":"Julie","ADDRESS":"12801 E 17th Ave, Room 1303","EMAIL":"julie.haines@cuanschutz.edu","PHONE":"3037243339"},

"STUDY":{"STUDY_TITLE":"The COVIDome Explorer Researcher Portal","STUDY_SUMMARY":"COVID-19 pathology involves dysregulation of diverse molecular, cellular, and physiological processes. In order to expedite integrated and collaborative COVID-19 research, we completed multi-omics analysis of hospitalized COVID-19 patients including matched analysis of the whole blood transcriptome, plasma proteomics with two complementary platforms, cytokine profiling, plasma and red blood cell metabolomics, deep immune cell phenotyping by mass cytometry, and clinical data annotation. We refer to this multidimensional dataset as the COVIDome. We then created the COVIDome Explorer, an online researcher portal where the data can be analyzed and visualized in real time. We illustrate here the use of the COVIDome dataset through a multi-omics analysis of biosignatures associated with C-reactive protein (CRP), an established marker of poor prognosis in COVID-19, revealing associations between CRP levels and damage-associated molecular patterns, depletion of protective serpins, and mitochondrial metabolism dysregulation. We expect that the COVIDome Explorer will rapidly accelerate data sharing, hypothesis testing, and discoveries worldwide.","INSTITUTE":"University of Colorado Anschutz Medical Campus","LAST_NAME":"Haines","FIRST_NAME":"Julie","ADDRESS":"12801 E 17th Ave, Room 1303","EMAIL":"julie.haines@cuanschutz.edu","PHONE":"3037243339"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"CUcovID_0043",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"21"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-40"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0067",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-72"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0092",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-101"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0085",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-92"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0101",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-113"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0057",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-58"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0026",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"53"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-19"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0052",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"43"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-53"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0098",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"61"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-110"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0049",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"50"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-50"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0039",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"38"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-35"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0087",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"21"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-94"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0047",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"59"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-47"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0070",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"69"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-75"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0089",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"60"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-97"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0002",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"44"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-22"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0084",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"25"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-91"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0035",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"23"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-30"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0088",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-96"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0024",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-16"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0056",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-57"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0082",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-89"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0081",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"25"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-88"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0086",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"23"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-93"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0062",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-66"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0031",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"50"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-26"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0095",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"76"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-106"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0019",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"60"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-07"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0102",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"57"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-114"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0045",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"46"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-42"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0003",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"29"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-44"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0090",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"43"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-98"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0096",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"43"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-107"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0011",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"79"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-17"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0046",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"54"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-46"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0080",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"35"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-86"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0071",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"49"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-76"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0009",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"25"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-103"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0059",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"55"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-62"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0018",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"45"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-05"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0029",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"47"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-24"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0077",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"49"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-83"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0065",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"34"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-69"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0012",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"42"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-45"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0041",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"33"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-37"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0040",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"61"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-36"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0075",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"41"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-81"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0072",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"67"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-77"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0063",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"43"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-67"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0069",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"56"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-74"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0028",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"34"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-23"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0105",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"59"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-118"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0022",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"39"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-12"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0091",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"35"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-100"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0083",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"47"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-90"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0079",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"34"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-85"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0021",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"24"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-10"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0038",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"39"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-33"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0093",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"26"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-102"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0094",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"31"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-104"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0006",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"41"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-117"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0017",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"36"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-04"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0044",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"80"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-41"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0073",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"54"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-78"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0023",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"63"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-14"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0027",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"36"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-20"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0097",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"71"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-109"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0037",
"Factors":{"COVID_Status":"Negative","Sex":"Female","Age":"30"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-32"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0048",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"60"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-48"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0025",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"56"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-18"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0076",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"65"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-82"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0036",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-31"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0066",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-71"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0050",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-51"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0078",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"under 20"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-84"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0054",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"46"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-55"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0109",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"30"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-123"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0064",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"66"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-68"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0014",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"53"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-65"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0068",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"52"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-73"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0030",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"60"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-25"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0074",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"61"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-80"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0061",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"51"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-64"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0106",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"21"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-119"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0100",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"23"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-112"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0051",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"42"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-52"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0008",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"39"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-03"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0055",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"54"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-56"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0016",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"41"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-02"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0042",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"55"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-38"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0020",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"50"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-09"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0104",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"79"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-116"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0033",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"29"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-28"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0013",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"42"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-49"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0108",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"45"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-122"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0010",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"71"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-95"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0005",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"61"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-59"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0001",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"77"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-08"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0007",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"28"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-15"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0060",
"Factors":{"COVID_Status":"Positive","Sex":"Female","Age":"38"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-63"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0004",
"Factors":{"COVID_Status":"Negative","Sex":"Male","Age":"38"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-34"}
},
{
"Subject ID":"-",
"Sample ID":"CUcovID_0107",
"Factors":{"COVID_Status":"Positive","Sex":"Male","Age":"26"},
"Additional sample data":{"RAW_FILE_NAME":"COV2-120"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Study design, participant recruitment, and clinical data capture. Research participants were recruited and consented for participation in the COVID Biobank of the University of Colorado Anschutz Medical Campus [Colorado Multiple Institutional Review Board (COMIRB) Protocol # 20-0685]. Data was generated from deidentified biospecimens and linked to demographics and clinical metadata procured through the Health Data Compass of the University of Colorado under COMIRB Protocol # 20-1700. Participants were hospitalized either at Children’s Hospital Colorado or the University of Colorado Hospital. COVID-19 status was defined by a positive PCR reaction and/or antibody test. Cohort characteristics can be found in Supp. File 1. METHOD DETAILS. Blood processing. Blood samples were collected into EDTA tubes, PAXgene RNA, and sodium heparin tubes. After centrifugation, EDTA plasma was used for MS proteomics, SOMAscan® proteomics, as well as multiplex immunoassays using MSD technology for both cytokine profiles and seroconversion assays. From sodium heparin tubes, PBMCs were obtained by the Ficoll gradient method before cryopreservation and assembly of batches for MC analysis.","SAMPLE_TYPE":"Blood (plasma)"},

"TREATMENT":{"TREATMENT_SUMMARY":"n/a"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were thawed on ice and extracted via a modified Folch method (chloroform/methanol/water 8:4:3), which completely inactivates other coronaviruses, such as MERS-CoV. Briefly, 20 μL of sample was diluted in 130 μL of LC-MS grade water, 600 μL of ice-cold chloroform/methanol (2:1) was added, and the samples were vortexed for 10 seconds. Samples were then incubated at 4°C for 5 minutes, quickly vortexed (5 seconds), and centrifuged at 14,000 g for 10 minutes at 4°C. The top (i.e., aqueous) phase was transferred to a new tube for metabolomics analysis and flash frozen. The bottom (i.e., organic) phase was transferred to a new tube for lipidomics analysis, then dried under N2 flow. UHPLC-MS metabolomics. Analyses were performed using a Vanquish UHPLC coupled online to a Q Exactive high resolution mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). Samples (10 uL per injection) were randomized and analyzed in positive and negative electrospray ionization modes (separate runs) using a 5-minute C18 gradient on a Kinetex C18 column (Phenomenex) as described (Nemkov et al., 2019). Data were analyzed using Maven (Princeton University, Princeton, NJ, USA) in conjunction with the KEGG database and an in-house standard library.","PROCESSING_STORAGE_CONDITIONS":"4℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"After sample randomization, 10 μL of extracts were injected into a Thermo Vanquish UHPLC system (San Jose, CA, USA) and resolved on a Kinetex C18 column (150 × 2.1 mm, 1.7 μm, Phenomenex, Torrance, CA, USA) at 450 μL/min through a 5 min gradient from 5 to 95% organic solvent B (mobile phases: A = water, 0.1% formic acid; B = acetonitrile, 0.1% formic acid) in positive ion mode. Solvent gradient: 0-0.5 min 5% B, 0.5-1.1 min 5-95% B, 1.1-2.75 min hold at 95% B, 2.75-3 min 95-5% B, 3-5 min hold at 5% B.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Phenomenex Kinetex C18 2.1 x 150 mm, 1.7 um","FLOW_RATE":"450 ul/min","COLUMN_TEMPERATURE":"45"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Resolution 70,000, scan range 65-900 m/z, maximum injection time 200 ms, microscans 2, automatic gain control (AGC) 3 x 10^6 ions, source voltage 4.0 kV, capillary temperature 320 C, and sheath gas 45, auxiliary gas 15, and sweep gas 0 (all nitrogen)."},

"MS_METABOLITE_DATA":{
"Units":"peak area",

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}

}