{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001965","ANALYSIS_ID":"AN003203","VERSION":"1","CREATED_ON":"October 27, 2021, 9:52 am"},

"PROJECT":{"PROJECT_TITLE":"Proteomics to Unveil Orchestration of Photorespiration and Central Carbon Pathway in Microchloropsis gaditana NIES 2587","PROJECT_TYPE":"Time Course VLC HC Metabolome","PROJECT_SUMMARY":"Photosynthetic organisms have evolved and adapted strategies to overcome the limiting concentrations of CO2. In this regard, the CO2-concentrating mechanism (CCM) developed by microalgae implies an efficient machinery to acquire CO2 in limiting environment. Inorganic carbon transporters channelize CO2 towards Rubisco, however, there are significant differences in the CCM of some species and it is obscurely understood. In the present study, we performed qualitative metabolomics and proteomics on Microchloropsis gaditana, under the influence of very-low CO2 (VLC; 300 ppm, or 0.03%) and high CO2 (HC; 30,000 ppm, or 3% v/v) at the time intervals of 0, 6, 12 and 24 hrs. Our results demonstrate that HC supplementation channelizes the carbon flux towards enhancing the biomass yield, increasing up to 1.7-fold. Cyclic electron flow driven (CEF) by PSI confers energy to the cells in the case of VLC in the initial acclimatization stage. Our qualitative metabolomic analyses has identified nearly 35 essential metabolites among which significant fold-change was observed as a photorespiratory by-product, glycolate, in VLC resulting in delayed growth and lower biomass. Whole cell proteomics study was performed in M. gaditana in both VLC and HC conditions and a total of 998 proteins were identified. Cells in VLC, undergoes dynamic changes to activate biophysical CCM with the help of bicarbonate transporters. In conclusion, comprehensive changes occur inside the cell that consequently mediate the assimilation and regulation of carbon metabolic loadout such that it favours fatty acid biosynthesis in HC. In conclusion, our emphasis is to delineate carbon assimilation in M. gaditana with the help of advanced multi-omics tools and provide translational approach for the enhanced production of biofuels and biorenewables.","INSTITUTE":"International Centre for Genetic Engineering and Biotechnology","DEPARTMENT":"Integrative Biotechnology","LABORATORY":"Omics of Algae","LAST_NAME":"Jutur","FIRST_NAME":"Pannaga Pavan","ADDRESS":"Omics of Algae Group, ICGEB campus, Aruna Asaf Ali Marg, New Delhi, Delhi, 110070, India","EMAIL":"jppavan@icgeb.res.in","PHONE":"26781358"},

"STUDY":{"STUDY_TITLE":"Integration of Metabolomics and Proteomics to Unveil Orchestration of Photorespiration and Central Carbon Pathway in Microchloropsis gaditana NIES 2587","STUDY_TYPE":"Time Course VLC HC Metabolome","STUDY_SUMMARY":"Photosynthetic organisms have evolved and adapted strategies to overcome the limiting concentrations of CO2. In this regard, the CO2-concentrating mechanism (CCM) developed by microalgae implies an efficient machinery to acquire CO2 in limiting environment. Inorganic carbon transporters channelize CO2 towards Rubisco, however, there are significant differences in the CCM of some species and it is obscurely understood. In the present study, we performed qualitative metabolomics and proteomics on Microchloropsis gaditana, under the influence of very-low CO2 (VLC; 300 ppm, or 0.03%) and high CO2 (HC; 30,000 ppm, or 3% v/v) at the time intervals of 0, 6, 12 and 24 hrs. Our results demonstrate that HC supplementation channelizes the carbon flux towards enhancing the biomass yield, increasing up to 1.7-fold. Cyclic electron flow driven (CEF) by PSI confers energy to the cells in the case of VLC in the initial acclimatization stage. Our qualitative metabolomic analyses has identified nearly 35 essential metabolites among which significant fold-change was observed as a photorespiratory by-product, glycolate, in VLC resulting in delayed growth and lower biomass. Whole cell proteomics study was performed in M. gaditana in both VLC and HC conditions and a total of 998 proteins were identified. Cells in VLC, undergoes dynamic changes to activate biophysical CCM with the help of bicarbonate transporters. In conclusion, comprehensive changes occur inside the cell that consequently mediate the assimilation and regulation of carbon metabolic loadout such that it favours fatty acid biosynthesis in HC. In conclusion, our emphasis is to delineate carbon assimilation in M. gaditana with the help of advanced multi-omics tools and provide translational approach for the enhanced production of biofuels and biorenewables.","INSTITUTE":"International Centre for Genetic Engineering and Biotechnology","DEPARTMENT":"Integrative Biotechnology","LABORATORY":"Omics of Algae","LAST_NAME":"Jutur","FIRST_NAME":"Pavan","ADDRESS":"Omics of Algae Lab, 2nd Floor, New Building, ICGEB, Aruna Asaf Ali Marg, New Delhi","EMAIL":"jppavan@gmail.com","PHONE":"01126741358"},

"SUBJECT":{"SUBJECT_TYPE":"Other organism","SUBJECT_SPECIES":"Microchloropsis"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"VLC_6hr_1",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_6hr_1"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_6hr_2",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_6hr_2"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_6hr_3",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_6hr_3"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_12hr_1",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_12hr_1"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_12hr_2",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_12hr_2"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_12hr_3",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_12hr_3"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_24hr_1",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_24hr_1"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_24hr_2",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_24hr_2"}
},
{
"Subject ID":"-",
"Sample ID":"VLC_24hr_3",
"Factors":{"Treatment":"VLC"},
"Additional sample data":{"RAW_FILE_NAME":"VLC_24hr_3"}
},
{
"Subject ID":"-",
"Sample ID":"HC_6hr_1",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_6hr_1"}
},
{
"Subject ID":"-",
"Sample ID":"HC_6hr_2",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_6hr_2"}
},
{
"Subject ID":"-",
"Sample ID":"HC_6hr_3",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_6hr_3"}
},
{
"Subject ID":"-",
"Sample ID":"HC_12hr_1",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_12hr_1"}
},
{
"Subject ID":"-",
"Sample ID":"HC_12hr_2",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_12hr_2"}
},
{
"Subject ID":"-",
"Sample ID":"HC_12hr_3",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_12hr_3"}
},
{
"Subject ID":"-",
"Sample ID":"HC_24hr_1",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_24hr_1"}
},
{
"Subject ID":"-",
"Sample ID":"HC_24hr_2",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_24hr_2"}
},
{
"Subject ID":"-",
"Sample ID":"HC_24hr_3",
"Factors":{"Treatment":"HC"},
"Additional sample data":{"RAW_FILE_NAME":"HC_24hr_3"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Marine microalgae Microchloropsis gaditana NIES 2587 is procured from Microbial Culture Collection, National Institute for Environmental Studies (NIES), Tsukuba, Japan. The strain was grown in minimal medium F/2 (Guillard and Ryther, 1962) under a light regime of 16:8 h and an illumination of 150 µmol m−2 s−1 photosynthetically active radiation (PAR) in a multi-cultivator MC 1000-OD (Photon Systems Instruments, Czech Republic) with a flow rate of 800 mL min-1 with continuous bubbling of air at 24 °C.","SAMPLE_TYPE":"Algae"},

"TREATMENT":{"TREATMENT_SUMMARY":"Microchloropsis gaditana cells were grown in a Multicultivator in the presence of very-low CO2 (300 ppm) and high CO2 (30,000 ppm) for 24 hours with an illumination intensity of 150 uE.","TREATMENT_COMPOUND":"CO2"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis.","PROCESSING_STORAGE_CONDITIONS":"Described in summary","EXTRACTION_METHOD":"Sonication","SAMPLE_DERIVATIZATION":"MSTFA","SAMPLE_SPIKING":"Ribitol (Internal Standard)"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"GC-triple quadrupole analysis was performed on an HP-5 gas chromatograph with standard liners containing glass wool in split mode (1:5) at 250°C injector temperature. The GC was operated at constant flow of 1 ml/min helium on a 30-m, 0.25-mm i.d., 0.25-μm HP-5 column, a start temperature of 60°C, 3 min isothermal, temperature ramping by 5°C/min to 180°C, 3 min isothermal and finally temperature ramping of 10°C/min to 310°C.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Agilent HP5-MS (30m x 0.25mm, 0.25 um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 7890A","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Mass Hunter"},

"MS_METABOLITE_DATA":{
"Units":"Area",

"Data":[{"Metabolite":"Alanine","VLC_6hr_1":"3244387","VLC_6hr_2":"1746127","VLC_6hr_3":"3273323","VLC_12hr_1":"1181743","VLC_12hr_2":"9725067","VLC_12hr_3":"26019342","VLC_24hr_1":"2164276","VLC_24hr_2":"699854","VLC_24hr_3":"2625316","HC_6hr_1":"15864880","HC_6hr_2":"5150896","HC_6hr_3":"28353606","HC_12hr_1":"4777717","HC_12hr_2":"10964164","HC_12hr_3":"9319152","HC_24hr_1":"64136868","HC_24hr_2":"12459556","HC_24hr_3":"578467"},{"Metabolite":"Glutamine","VLC_6hr_1":"110463.2","VLC_6hr_2":"110463.2","VLC_6hr_3":"110463.2","VLC_12hr_1":"110463.2","VLC_12hr_2":"110463.2","VLC_12hr_3":"110463.2","VLC_24hr_1":"110463.2","VLC_24hr_2":"110463.2","VLC_24hr_3":"110463.2","HC_6hr_1":"110463.2","HC_6hr_2":"110463.2","HC_6hr_3":"110463.2","HC_12hr_1":"110463.2","HC_12hr_2":"110463.2","HC_12hr_3":"110463.2","HC_24hr_1":"552316","HC_24hr_2":"110463.2","HC_24hr_3":"110463.2"},{"Metabolite":"Glycine","VLC_6hr_1":"2391243","VLC_6hr_2":"10508067","VLC_6hr_3":"570653","VLC_12hr_1":"7083560","VLC_12hr_2":"699441","VLC_12hr_3":"330813","VLC_24hr_1":"625713","VLC_24hr_2":"714878","VLC_24hr_3":"371842","HC_6hr_1":"7567633","HC_6hr_2":"66162.6","HC_6hr_3":"823216","HC_12hr_1":"66162.6","HC_12hr_2":"569479","HC_12hr_3":"66162.6","HC_24hr_1":"25052594","HC_24hr_2":"1757040","HC_24hr_3":"4643990"},{"Metabolite":"Histidine","VLC_6hr_1":"105537.8","VLC_6hr_2":"105537.8","VLC_6hr_3":"105537.8","VLC_12hr_1":"105537.8","VLC_12hr_2":"105537.8","VLC_12hr_3":"105537.8","VLC_24hr_1":"105537.8","VLC_24hr_2":"105537.8","VLC_24hr_3":"105537.8","HC_6hr_1":"105537.8","HC_6hr_2":"105537.8","HC_6hr_3":"105537.8","HC_12hr_1":"105537.8","HC_12hr_2":"105537.8","HC_12hr_3":"105537.8","HC_24hr_1":"527689","HC_24hr_2":"105537.8","HC_24hr_3":"105537.8"},{"Metabolite":"Leucine","VLC_6hr_1":"74303","VLC_6hr_2":"1654095","VLC_6hr_3":"74303","VLC_12hr_1":"74303","VLC_12hr_2":"74303","VLC_12hr_3":"608053","VLC_24hr_1":"74303","VLC_24hr_2":"371515","VLC_24hr_3":"74303","HC_6hr_1":"41397018","HC_6hr_2":"74303","HC_6hr_3":"74303","HC_12hr_1":"1804657","HC_12hr_2":"469832","HC_12hr_3":"74303","HC_24hr_1":"74303","HC_24hr_2":"74303","HC_24hr_3":"2738516"},{"Metabolite":"Lysine","VLC_6hr_1":"529861.4","VLC_6hr_2":"529861.4","VLC_6hr_3":"529861.4","VLC_12hr_1":"529861.4","VLC_12hr_2":"529861.4","VLC_12hr_3":"529861.4","VLC_24hr_1":"529861.4","VLC_24hr_2":"529861.4","VLC_24hr_3":"529861.4","HC_6hr_1":"529861.4","HC_6hr_2":"529861.4","HC_6hr_3":"529861.4","HC_12hr_1":"529861.4","HC_12hr_2":"529861.4","HC_12hr_3":"529861.4","HC_24hr_1":"2649307","HC_24hr_2":"529861.4","HC_24hr_3":"529861.4"},{"Metabolite":"Proline","VLC_6hr_1":"20509465","VLC_6hr_2":"16241432","VLC_6hr_3":"11449789","VLC_12hr_1":"8091030","VLC_12hr_2":"9501006","VLC_12hr_3":"9152727","VLC_24hr_1":"20766175","VLC_24hr_2":"18148565","VLC_24hr_3":"7783481","HC_6hr_1":"27385525","HC_6hr_2":"16012782","HC_6hr_3":"16139087","HC_12hr_1":"16080179","HC_12hr_2":"8182184","HC_12hr_3":"29752313","HC_24hr_1":"8158507","HC_24hr_2":"60703051","HC_24hr_3":"17676351"},{"Metabolite":"Valine","VLC_6hr_1":"102214.8","VLC_6hr_2":"102214.8","VLC_6hr_3":"102214.8","VLC_12hr_1":"102214.8","VLC_12hr_2":"102214.8","VLC_12hr_3":"102214.8","VLC_24hr_1":"102214.8","VLC_24hr_2":"102214.8","VLC_24hr_3":"102214.8","HC_6hr_1":"1410017","HC_6hr_2":"102214.8","HC_6hr_3":"102214.8","HC_12hr_1":"102214.8","HC_12hr_2":"102214.8","HC_12hr_3":"1847273","HC_24hr_1":"1055414","HC_24hr_2":"553859","HC_24hr_3":"511074"},{"Metabolite":"Galactose","VLC_6hr_1":"158232","VLC_6hr_2":"791160","VLC_6hr_3":"158232","VLC_12hr_1":"158232","VLC_12hr_2":"158232","VLC_12hr_3":"158232","VLC_24hr_1":"158232","VLC_24hr_2":"158232","VLC_24hr_3":"158232","HC_6hr_1":"1879705","HC_6hr_2":"2589335","HC_6hr_3":"158232","HC_12hr_1":"158232","HC_12hr_2":"46977004","HC_12hr_3":"158232","HC_24hr_1":"2306522","HC_24hr_2":"1087734","HC_24hr_3":"1209505"},{"Metabolite":"Glucose","VLC_6hr_1":"126876","VLC_6hr_2":"1419256","VLC_6hr_3":"1006619","VLC_12hr_1":"126876","VLC_12hr_2":"126876","VLC_12hr_3":"126876","VLC_24hr_1":"943361","VLC_24hr_2":"126876","VLC_24hr_3":"634380","HC_6hr_1":"5269071","HC_6hr_2":"4043480","HC_6hr_3":"44232477","HC_12hr_1":"126876","HC_12hr_2":"2405102","HC_12hr_3":"1660080","HC_24hr_1":"1075098","HC_24hr_2":"60328823","HC_24hr_3":"14870736"},{"Metabolite":"Glycerol","VLC_6hr_1":"745008","VLC_6hr_2":"871613","VLC_6hr_3":"750859","VLC_12hr_1":"1007172","VLC_12hr_2":"1007172","VLC_12hr_3":"1282731","VLC_24hr_1":"1709879","VLC_24hr_2":"1874981","VLC_24hr_3":"516369","HC_6hr_1":"885973","HC_6hr_2":"3108184","HC_6hr_3":"655707","HC_12hr_1":"1306483","HC_12hr_2":"586422","HC_12hr_3":"549440","HC_24hr_1":"2172715","HC_24hr_2":"1208948","HC_24hr_3":"1320905"},{"Metabolite":"Maltose","VLC_6hr_1":"3444494","VLC_6hr_2":"89724.4","VLC_6hr_3":"89724.4","VLC_12hr_1":"89724.4","VLC_12hr_2":"89724.4","VLC_12hr_3":"89724.4","VLC_24hr_1":"89724.4","VLC_24hr_2":"89724.4","VLC_24hr_3":"89724.4","HC_6hr_1":"89724.4","HC_6hr_2":"2345229","HC_6hr_3":"497986","HC_12hr_1":"1934196","HC_12hr_2":"89724.4","HC_12hr_3":"89724.4","HC_24hr_1":"89724.4","HC_24hr_2":"448622","HC_24hr_3":"875292"},{"Metabolite":"Acetic acid","VLC_6hr_1":"4462784","VLC_6hr_2":"4462784","VLC_6hr_3":"782991","VLC_12hr_1":"153860.8","VLC_12hr_2":"153860.8","VLC_12hr_3":"153860.8","VLC_24hr_1":"8125303","VLC_24hr_2":"8681611","VLC_24hr_3":"7618974","HC_6hr_1":"769304","HC_6hr_2":"1453309","HC_6hr_3":"1475354","HC_12hr_1":"3190188","HC_12hr_2":"1301083","HC_12hr_3":"8031965","HC_24hr_1":"60828001","HC_24hr_2":"8256980","HC_24hr_3":"9193593"},{"Metabolite":"Carbonic acid","VLC_6hr_1":"461738","VLC_6hr_2":"1099601","VLC_6hr_3":"1623589","VLC_12hr_1":"92347.6","VLC_12hr_2":"1971034","VLC_12hr_3":"843992","VLC_24hr_1":"1176226","VLC_24hr_2":"553231","VLC_24hr_3":"92347.6","HC_6hr_1":"92347.6","HC_6hr_2":"565156","HC_6hr_3":"92347.6","HC_12hr_1":"92347.6","HC_12hr_2":"92347.6","HC_12hr_3":"92347.6","HC_24hr_1":"547746","HC_24hr_2":"92347.6","HC_24hr_3":"92347.6"},{"Metabolite":"Fumaric acid","VLC_6hr_1":"4688012","VLC_6hr_2":"96786.2","VLC_6hr_3":"96786.2","VLC_12hr_1":"96786.2","VLC_12hr_2":"96786.2","VLC_12hr_3":"96786.2","VLC_24hr_1":"96786.2","VLC_24hr_2":"96786.2","VLC_24hr_3":"96786.2","HC_6hr_1":"96786.2","HC_6hr_2":"1062453","HC_6hr_3":"96786.2","HC_12hr_1":"483931","HC_12hr_2":"96786.2","HC_12hr_3":"96786.2","HC_24hr_1":"4973896","HC_24hr_2":"96786.2","HC_24hr_3":"21085272"},{"Metabolite":"Glycolic acid","VLC_6hr_1":"1459950","VLC_6hr_2":"1395142","VLC_6hr_3":"3797136","VLC_12hr_1":"1338589","VLC_12hr_2":"1338589","VLC_12hr_3":"1275177","VLC_24hr_1":"1267217","VLC_24hr_2":"4057096","VLC_24hr_3":"1122408","HC_6hr_1":"224481.6","HC_6hr_2":"224481.6","HC_6hr_3":"224481.6","HC_12hr_1":"224481.6","HC_12hr_2":"224481.6","HC_12hr_3":"224481.6","HC_24hr_1":"224481.6","HC_24hr_2":"224481.6","HC_24hr_3":"224481.6"},{"Metabolite":"Lactic acid","VLC_6hr_1":"781349","VLC_6hr_2":"714332","VLC_6hr_3":"90069.2","VLC_12hr_1":"1053267","VLC_12hr_2":"90069.2","VLC_12hr_3":"2145235","VLC_24hr_1":"2394128","VLC_24hr_2":"733093","VLC_24hr_3":"450346","HC_6hr_1":"1008956","HC_6hr_2":"90069.2","HC_6hr_3":"688446","HC_12hr_1":"3230608","HC_12hr_2":"2673536","HC_12hr_3":"2308500","HC_24hr_1":"4692327","HC_24hr_2":"3627775","HC_24hr_3":"1693333"},{"Metabolite":"Malonic acid","VLC_6hr_1":"65830.8","VLC_6hr_2":"65830.8","VLC_6hr_3":"757696","VLC_12hr_1":"65830.8","VLC_12hr_2":"65830.8","VLC_12hr_3":"1251250","VLC_24hr_1":"65830.8","VLC_24hr_2":"329154","VLC_24hr_3":"1329371","HC_6hr_1":"65830.8","HC_6hr_2":"1376083","HC_6hr_3":"65830.8","HC_12hr_1":"65830.8","HC_12hr_2":"65830.8","HC_12hr_3":"65830.8","HC_24hr_1":"65830.8","HC_24hr_2":"439845","HC_24hr_3":"65830.8"},{"Metabolite":"Oxalic acid","VLC_6hr_1":"2521220","VLC_6hr_2":"2852884","VLC_6hr_3":"272544.8","VLC_12hr_1":"2480696","VLC_12hr_2":"2480696","VLC_12hr_3":"2469891","VLC_24hr_1":"2397039","VLC_24hr_2":"272544.8","VLC_24hr_3":"4384701","HC_6hr_1":"4099165","HC_6hr_2":"2470513","HC_6hr_3":"2819058","HC_12hr_1":"2751527","HC_12hr_2":"272544.8","HC_12hr_3":"2691746","HC_24hr_1":"3136111","HC_24hr_2":"2123615","HC_24hr_3":"1362724"},{"Metabolite":"Succinic acid","VLC_6hr_1":"3908088","VLC_6hr_2":"898527","VLC_6hr_3":"84755.8","VLC_12hr_1":"84755.8","VLC_12hr_2":"84755.8","VLC_12hr_3":"5327993","VLC_24hr_1":"478109","VLC_24hr_2":"84755.8","VLC_24hr_3":"562563","HC_6hr_1":"84755.8","HC_6hr_2":"423779","HC_6hr_3":"480662","HC_12hr_1":"953411","HC_12hr_2":"84755.8","HC_12hr_3":"1503036","HC_24hr_1":"1507483","HC_24hr_2":"84755.8","HC_24hr_3":"1626719"},{"Metabolite":"α-Ketoglutaric 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"Metabolites":[{"metabolite_name":"Alanine"},{"metabolite_name":"Glutamine"},{"metabolite_name":"Glycine"},{"metabolite_name":"Histidine"},{"metabolite_name":"Leucine"},{"metabolite_name":"Lysine"},{"metabolite_name":"Proline"},{"metabolite_name":"Valine"},{"metabolite_name":"Galactose"},{"metabolite_name":"Glucose"},{"metabolite_name":"Glycerol"},{"metabolite_name":"Maltose"},{"metabolite_name":"Acetic acid"},{"metabolite_name":"Carbonic acid"},{"metabolite_name":"Fumaric acid"},{"metabolite_name":"Glycolic acid"},{"metabolite_name":"Lactic acid"},{"metabolite_name":"Malonic acid"},{"metabolite_name":"Oxalic acid"},{"metabolite_name":"Succinic acid"},{"metabolite_name":"α-Ketoglutaric acid"},{"metabolite_name":"Ethanolamine"},{"metabolite_name":"Myo-inositol"},{"metabolite_name":"Phosphoric acid"},{"metabolite_name":"Phytol"},{"metabolite_name":"Trehalose"},{"metabolite_name":"Eicosanoic acid"},{"metabolite_name":"Eicosatrienoic acid"},{"metabolite_name":"EPA"},{"metabolite_name":"Heptadecanoic acid"},{"metabolite_name":"Hexadecanoic acid"},{"metabolite_name":"α-Linolenic acid"},{"metabolite_name":"Octadecanoic acid"},{"metabolite_name":"Ergosterol"},{"metabolite_name":"Stigmasterol"},{"metabolite_name":"α-Tocopherol"}]
}

}