{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001979","ANALYSIS_ID":"AN003229","VERSION":"1","CREATED_ON":"September 17, 2021, 9:59 am"},

"PROJECT":{"PROJECT_TITLE":"Gene-therapy enhances CSF’s anti-oxidative activity to mitigate chemotherapy side effects","PROJECT_TYPE":"Anti-oxidative Metabolism Measurement in CSF of MTX-treated mice under gene therapy by quantitative LC/MS method (part VI)","PROJECT_SUMMARY":"In order to test the protective activity of SOD3 levels on the redox state of the CSF of MTX-treated mice, targeted metabolomics on CSF was employed at different timepoints after treatment, on mice of both sexes, at various levels of impacted hSOD3 expression.","INSTITUTE":"Boston Childrens Hospital","DEPARTMENT":"Pathology","LABORATORY":"Naama Kanarek","LAST_NAME":"Petrova","FIRST_NAME":"Boryana","ADDRESS":"300 Longwood Av, Boston, MA, 2115, USA","EMAIL":"boryana.petrova@childrens.harvard.edu","PHONE":"6173557433"},

"STUDY":{"STUDY_TITLE":"Anti-oxidation metabolism measurement in mammalian cells and tissues by quantitative LC/MS method (VI)","STUDY_SUMMARY":"Anti-oxidation metabolism measurement in mammalian cells and tissues by quantitative LC/MS method of mouse ctrl CSF.","INSTITUTE":"Boston Children's Hospital, Harvard Medical School","LAST_NAME":"Petrova","FIRST_NAME":"Boryana","ADDRESS":"300 Longwood Ave","EMAIL":"boryana.petrova@childrens.harvard.edu","PHONE":"6173557433"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Male and female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 1",
"Factors":{"Factor":"PBS-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ441_20210403144300","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 2",
"Factors":{"Factor":"PBS-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ442_20210403134529","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 3",
"Factors":{"Factor":"PBS-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ443_20210402205911","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 4",
"Factors":{"Factor":"PBS-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ444_20210403080025","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 5",
"Factors":{"Factor":"PBS-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ445_20210403060526","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 6",
"Factors":{"Factor":"PBS-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ446_20210403102412","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 7",
"Factors":{"Factor":"PBS-7"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ447_20210403193036","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 8",
"Factors":{"Factor":"PBS-8"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ448_20210403011757","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 9",
"Factors":{"Factor":"PBS-9"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ449_20210402151403","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 10",
"Factors":{"Factor":"PBS+MTX-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ450_20210403131645","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 11",
"Factors":{"Factor":"PBS+MTX-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ451_20210403115029","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 12",
"Factors":{"Factor":"PBS+MTX-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ452_20210402134749","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 13",
"Factors":{"Factor":"PBS+MTX-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ453_20210403212538","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 14",
"Factors":{"Factor":"PBS+MTX-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ454_20210402203026","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 15",
"Factors":{"Factor":"PBS+MTX-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ455_20210403195922","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 16",
"Factors":{"Factor":"PBS+MTX-7"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ456_20210403154031","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 17",
"Factors":{"Factor":"PBS+MTX-8"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ457_20210403085756","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 18",
"Factors":{"Factor":"PBS+MTX-9"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ458_20210402232257","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 19",
"Factors":{"Factor":"Null GFP-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ459_20210403105257","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 20",
"Factors":{"Factor":"Null GFP-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ460_20210403151146","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 21",
"Factors":{"Factor":"Null GFP-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ461_20210403190151","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 22",
"Factors":{"Factor":"Null GFP-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ462_20210403041027","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 23",
"Factors":{"Factor":"Null GFP-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ463_20210402225413","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 24",
"Factors":{"Factor":"Null GFP-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ464_20210403050755","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 25",
"Factors":{"Factor":"Null GFP-7"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ465_20210402222527","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 26",
"Factors":{"Factor":"Null GFP+MTX-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ466_20210403021527","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 27",
"Factors":{"Factor":"Null GFP+MTX-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ467_20210403095526","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 28",
"Factors":{"Factor":"Null GFP+MTX-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ468_20210402235142","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 29",
"Factors":{"Factor":"Null GFP+MTX-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ469_20210403053641","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 30",
"Factors":{"Factor":"Null GFP+MTX-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ470_20210403031257","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 31",
"Factors":{"Factor":"Null GFP+MTX-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ471_20210402200142","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 32",
"Factors":{"Factor":"Null GFP+MTX-7"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ472_20210402164022","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 33",
"Factors":{"Factor":"Null GFP+MTX-8"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ473_20210403180419","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 34",
"Factors":{"Factor":"CAG-GFP-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ474_20210402125018","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 35",
"Factors":{"Factor":"CAG-GFP-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ475_20210402180641","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 36",
"Factors":{"Factor":"CAG-GFP-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ476_20210402212756","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 37",
"Factors":{"Factor":"CAG-GFP-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ477_20210402161138","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 38",
"Factors":{"Factor":"CAG-GFP-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ478_20210403043912","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 39",
"Factors":{"Factor":"CAG-GFP-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ479_20210403014642","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 40",
"Factors":{"Factor":"CAG-GFP-7"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ480_20210402190411","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 41",
"Factors":{"Factor":"CAG-GFP-8"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ481_20210403092641","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 42",
"Factors":{"Factor":"CAG-GFP+MTX-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ482_20210403063410","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 43",
"Factors":{"Factor":"CAG-GFP+MTX-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ483_20210402183527","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 44",
"Factors":{"Factor":"CAG-GFP+MTX-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ484_20210402131904","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 45",
"Factors":{"Factor":"CAG-GFP+MTX-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ485_20210402154249","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 46",
"Factors":{"Factor":"CAG-GFP+MTX-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ486_20210403160917","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 47",
"Factors":{"Factor":"CAG-GFP+MTX-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ487_20210402144519","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 48",
"Factors":{"Factor":"CAG-GFP+MTX-7"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ488_20210403202807","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 49",
"Factors":{"Factor":"CMV-GFP-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ489_20210403082911","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 50",
"Factors":{"Factor":"CMV-GFP-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ490_20210403163805","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 51",
"Factors":{"Factor":"CMV-GFP-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ491_20210403173535","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 52",
"Factors":{"Factor":"CMV-GFP-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ492_20210403034141","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 53",
"Factors":{"Factor":"CMV-GFP-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ493_20210403070255","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 54",
"Factors":{"Factor":"CMV-GFP-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ494_20210403205651","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 55",
"Factors":{"Factor":"CMV-GFP+MTX-1"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ495_20210403004912","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 56",
"Factors":{"Factor":"CMV-GFP+MTX-2"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ496_20210403112143","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 57",
"Factors":{"Factor":"CMV-GFP+MTX-3"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ497_20210402193255","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 58",
"Factors":{"Factor":"CMV-GFP+MTX-4"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ498_20210403183305","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 59",
"Factors":{"Factor":"CMV-GFP+MTX-5"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ499_20210403002026","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 60",
"Factors":{"Factor":"CMV-GFP+MTX-6"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ500_20210402141633","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 61",
"Factors":{"Factor":"CMV-GFP+MTX-7"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ501_20210403141414","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 62",
"Factors":{"Factor":"CMV-GFP+MTX-8"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ502_20210402173755","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"Mouse CSF Ctrl 63",
"Factors":{"Factor":"CMV-GFP+MTX-9"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ503_20210403124800","other":"3ul CSF 200 FB reconstituted in 25ul water with QReSS","other":"folate buffer isoAAs GSH aminopterin","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1a",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_a_20210402112359","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1b",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_b_20210402170909","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1c",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_c_20210402215641","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1d",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_d_20210403024411","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1e",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_e_20210403073139","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1f",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_f_20210403121915","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1g",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_g_20210403170649","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1h",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ504_pool1x_h_20210403215423","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1/3 dilution",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ505_pool03x_20210403222310","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1/10 dilution a-2x",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ506_pool01x_2x_20210402122129","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1/10 dilution a",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ506_pool01x_a_20210402115245","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"pooled sample QC 1/10 dilution b",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_AJ506_pool01x_b_20210403225154","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"blank 1",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_blank1_20210402095740","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"blank 2",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_blank2_20210402102630","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"blank 3",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_blank3_20210402105515","other":"-","other":"-","chromatography":"HILIC"}
},
{
"Subject ID":"-",
"Sample ID":"blank 4",
"Factors":{"Factor":"-"},
"Additional sample data":{"RAW_FILE_NAME":"20210331_QEF1_HILIC_Aram_newGFPcontrols_MTX_blank4_20210403232038","other":"-","other":"-","chromatography":"HILIC"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"All animal studies were performed under the protocol approved by the Institutional Animal Care and Use Committee (IACUC) of Boston Children’s Hospital (BCH). CD-1 mice (timed pregnant and 6-8 weeks) and Sprague Dawley rats (6-8 weeks) were purchased (Charles River Laboratories). Male and female animals were used equally for all studies. All animals were housed in a 12h light-dark cycle with ad libitum access to food and water. CSF was collected from the cisterna magna and centrifuged at 10,000g for 10 min. at 4 °C to remove any tissue debris (38). CSF samples were flash frozen and stored at −80°C until use.","SAMPLE_TYPE":"CSF"},

"TREATMENT":{"TREATMENT_SUMMARY":"Mice were treated with PBS, PBS+ MTX, Null GFP with and without MTX, CAG-GFP with and without MTX, and CMV-GFP with and without MTX. buffers from study design were used for all respective samples. CD-1 mice (6-8 weeks) or Sprague Dawley rats (8 weeks) were given a single intravenous injection of 75 mg/kg MTX (Fisher Scientific, AAJ66364MD) dissolved in 0.9 % NaCl (Mountainside Medical, 0409-4888-10). MTX was freshly prepared for each experiment. MTX in mouse serum or CSF was assessed by MTX ELISA according to the manufacturer's instructions (Enzo Life Sciences, 142-0001)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"For characterization by mass spectrometry, CSF was acquired 4h, 24h, and 48h following a single 75 mg/kg MTX injection from 6-8 mice and flash frozen for further analysis. Per condition, 3 μl of CSF were extracted by brief sonication in 240 μl 100% methanol, supplemented with isotopically labeled internal standards (17 amino acids and reduced glutathione, Cambridge Isotope Laboratories, MSK-A2-1.2 and CNLM-6245-10) and 60 μl 20 mM Ellman’s reagent in water (Sigma-Aldrich, D8130). After centrifugation for 10min. at maximum speed on a benchtop centrifuge (Eppendorf) the cleared supernatant was dried using a nitrogen dryer and reconstituted in 30 µl water by brief sonication. Extracted metabolites were spun again and cleared supernatant was transferred in LC-MS microvials. A small amount of each sample was pooled and serially diluted 3 and 10-fold to be used as quality controls throughout the batch run. Two microliters (equivalent to 0.2 ul of CSF) of reconstituted sample were injected into a ZIC-pHILIC 150 × 2.1 mm (5 µm particle size) column (EMD Millipore) operated on a Dionex UltiMate 3000 UPLC system (Thermo Fisher Scientific). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 20 mM ammonium carbonate, 0.1% ammonium hydroxide. Gradient conditions were: linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B. The column oven and autosampler tray were held at 25 °C and 4 °C, respectively. The MS data acquisition was on a QExactive benchtop orbitrap mass spectrometer equipped with an Ion Max source and a HESI II probe and was performed in a range of m/z= 70–1000, with the resolution set at 70,000, the AGC target at 1x106, and the maximum injection time (Max IT) at 20 msec. For tSIM scans, the resolution was set at 70,000, the AGC target was 1x105, and the max IT was 100 msec. Relative quantitation of polar metabolites was performed with TraceFinder 4.1 (Thermo Fisher Scientific) using a 5 ppm mass tolerance and referencing an in-house library of chemical standards. Pooled samples and fractional dilutions were prepared as quality controls and only those metabolites were taken for further analysis, for which the correlation between the dilution factor and the peak area was >0.95 (high confidence metabolites). Normalization for biological material amounts was based on the total integrated peak area values of high-confidence metabolites within an experimental batch after normalizing to the averaged factor from all mean-centered chromatographic peak areas of isotopically labeled amino acids internal standards (Cambridge Isotope Laboratories). The data were Log transformed and Pareto scaled for MetaboAnalyst-based statistical or pathway analysis (41). We profiled 200 metabolites, 85 of which were detected in CSF and passed our quality control protocol."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","COLUMN_NAME":"EMD Millipore ZIC-HILIC (100 x 2.1 mm, 3.5 um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Exactive Plus Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Xcalibur + Tracefinder"},

"MS_METABOLITE_DATA":{
"Units":"ppm",

"Data":[{"Metabolite":"GSH-13C2_15N","Mouse CSF Ctrl 1":"N/F","Mouse CSF Ctrl 2":"N/F","Mouse CSF Ctrl 3":"N/F","Mouse CSF Ctrl 4":"N/F","Mouse CSF Ctrl 5":"N/F","Mouse CSF Ctrl 6":"N/F","Mouse CSF Ctrl 7":"N/F","Mouse CSF Ctrl 8":"N/F","Mouse CSF Ctrl 9":"N/F","Mouse CSF Ctrl 10":"N/F","Mouse CSF Ctrl 11":"N/F","Mouse CSF Ctrl 12":"N/F","Mouse CSF Ctrl 13":"N/F","Mouse CSF Ctrl 14":"N/F","Mouse CSF Ctrl 15":"N/F","Mouse CSF Ctrl 16":"N/F","Mouse CSF Ctrl 17":"N/F","Mouse CSF Ctrl 18":"N/F","Mouse CSF Ctrl 19":"N/F","Mouse CSF Ctrl 20":"N/F","Mouse CSF Ctrl 21":"N/F","Mouse CSF Ctrl 22":"N/F","Mouse CSF Ctrl 23":"N/F","Mouse CSF Ctrl 24":"N/F","Mouse CSF Ctrl 25":"N/F","Mouse CSF Ctrl 26":"N/F","Mouse CSF Ctrl 27":"N/F","Mouse CSF Ctrl 28":"N/F","Mouse CSF Ctrl 29":"N/F","Mouse CSF Ctrl 30":"N/F","Mouse CSF Ctrl 31":"N/F","Mouse CSF Ctrl 32":"N/F","Mouse CSF Ctrl 33":"N/F","Mouse CSF Ctrl 34":"N/F","Mouse CSF Ctrl 35":"N/F","Mouse CSF Ctrl 36":"N/F","Mouse CSF Ctrl 37":"N/F","Mouse CSF 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dilution a":"N/F","pooled sample QC 1/10 dilution b":"N/F","blank 1":"N/F","blank 2":"N/F","blank 3":"N/F","blank 4":"N/F"},{"Metabolite":"GSH_13C2-15N-Ellmans","Mouse CSF Ctrl 1":"3897674","Mouse CSF Ctrl 2":"3272502","Mouse CSF Ctrl 3":"2729500","Mouse CSF Ctrl 4":"4164179","Mouse CSF Ctrl 5":"4820161","Mouse CSF Ctrl 6":"3135990","Mouse CSF Ctrl 7":"5686300","Mouse CSF Ctrl 8":"2028539","Mouse CSF Ctrl 9":"1219497","Mouse CSF Ctrl 10":"5228198","Mouse CSF Ctrl 11":"5027629","Mouse CSF Ctrl 12":"1152474","Mouse CSF Ctrl 13":"4541579","Mouse CSF Ctrl 14":"1571656","Mouse CSF Ctrl 15":"4137268","Mouse CSF Ctrl 16":"4363462","Mouse CSF Ctrl 17":"3500808","Mouse CSF Ctrl 18":"1427368","Mouse CSF Ctrl 19":"3015480","Mouse CSF Ctrl 20":"4233792","Mouse CSF Ctrl 21":"4218514","Mouse CSF Ctrl 22":"3309275","Mouse CSF Ctrl 23":"859423","Mouse CSF Ctrl 24":"3651052","Mouse CSF Ctrl 25":"2339991","Mouse CSF Ctrl 26":"3142175","Mouse CSF Ctrl 27":"3177320","Mouse CSF Ctrl 28":"2462337","Mouse CSF Ctrl 29":"3638004","Mouse CSF Ctrl 30":"3742328","Mouse CSF Ctrl 31":"2353480","Mouse CSF Ctrl 32":"1569429","Mouse CSF Ctrl 33":"4070432","Mouse CSF Ctrl 34":"1646532","Mouse CSF Ctrl 35":"2665204","Mouse CSF Ctrl 36":"1825613","Mouse CSF Ctrl 37":"1071052","Mouse CSF Ctrl 38":"N/F","Mouse CSF Ctrl 39":"2262529","Mouse CSF Ctrl 40":"2120100","Mouse CSF Ctrl 41":"2902911","Mouse CSF Ctrl 42":"2935680","Mouse CSF Ctrl 43":"2075585","Mouse CSF Ctrl 44":"835563","Mouse CSF Ctrl 45":"859150","Mouse CSF Ctrl 46":"3443460","Mouse CSF Ctrl 47":"476765","Mouse CSF Ctrl 48":"3044060","Mouse CSF Ctrl 49":"2723376","Mouse CSF Ctrl 50":"2705087","Mouse CSF Ctrl 51":"3872250","Mouse CSF Ctrl 52":"2475790","Mouse CSF Ctrl 53":"1728059","Mouse CSF Ctrl 54":"3960959","Mouse CSF Ctrl 55":"1955687","Mouse CSF Ctrl 56":"3874866","Mouse CSF Ctrl 57":"422190","Mouse CSF Ctrl 58":"4184859","Mouse CSF Ctrl 59":"2127612","Mouse CSF Ctrl 60":"397549","Mouse CSF Ctrl 61":"3548800","Mouse CSF Ctrl 62":"464502","Mouse CSF Ctrl 63":"3518810","pooled sample QC 1a":"736850","pooled sample QC 1b":"1602170","pooled sample QC 1c":"2289267","pooled sample QC 1d":"2980622","pooled sample QC 1e":"3490771","pooled sample QC 1f":"4264756","pooled sample QC 1g":"4808487","pooled sample QC 1h":"5354530","pooled sample QC 1/3 dilution":"90076","pooled sample QC 1/10 dilution a-2x":"N/F","pooled sample QC 1/10 dilution a":"N/F","pooled sample QC 1/10 dilution b":"N/F","blank 1":"N/F","blank 2":"N/F","blank 3":"N/F","blank 4":"N/F"},{"Metabolite":"GSH","Mouse CSF Ctrl 1":"N/F","Mouse CSF Ctrl 2":"N/F","Mouse CSF Ctrl 3":"N/F","Mouse CSF Ctrl 4":"N/F","Mouse CSF Ctrl 5":"N/F","Mouse CSF Ctrl 6":"N/F","Mouse CSF Ctrl 7":"N/F","Mouse CSF Ctrl 8":"N/F","Mouse CSF Ctrl 9":"N/F","Mouse CSF Ctrl 10":"N/F","Mouse CSF Ctrl 11":"N/F","Mouse CSF Ctrl 12":"N/F","Mouse CSF Ctrl 13":"N/F","Mouse CSF Ctrl 14":"N/F","Mouse CSF Ctrl 15":"N/F","Mouse CSF Ctrl 16":"N/F","Mouse CSF Ctrl 17":"N/F","Mouse CSF Ctrl 18":"N/F","Mouse CSF Ctrl 19":"N/F","Mouse CSF Ctrl 20":"N/F","Mouse CSF Ctrl 21":"N/F","Mouse CSF Ctrl 22":"N/F","Mouse CSF Ctrl 23":"N/F","Mouse CSF Ctrl 24":"N/F","Mouse CSF Ctrl 25":"N/F","Mouse CSF Ctrl 26":"N/F","Mouse CSF Ctrl 27":"N/F","Mouse CSF Ctrl 28":"N/F","Mouse CSF Ctrl 29":"N/F","Mouse CSF Ctrl 30":"N/F","Mouse CSF Ctrl 31":"N/F","Mouse CSF Ctrl 32":"N/F","Mouse CSF Ctrl 33":"N/F","Mouse CSF Ctrl 34":"N/F","Mouse CSF Ctrl 35":"N/F","Mouse CSF Ctrl 36":"N/F","Mouse CSF Ctrl 37":"N/F","Mouse CSF Ctrl 38":"N/F","Mouse CSF Ctrl 39":"N/F","Mouse CSF Ctrl 40":"N/F","Mouse CSF Ctrl 41":"N/F","Mouse CSF Ctrl 42":"N/F","Mouse CSF Ctrl 43":"N/F","Mouse CSF Ctrl 44":"N/F","Mouse CSF Ctrl 45":"N/F","Mouse CSF Ctrl 46":"N/F","Mouse CSF Ctrl 47":"N/F","Mouse CSF Ctrl 48":"N/F","Mouse CSF Ctrl 49":"N/F","Mouse CSF Ctrl 50":"N/F","Mouse CSF Ctrl 51":"N/F","Mouse CSF Ctrl 52":"N/F","Mouse CSF Ctrl 53":"N/F","Mouse CSF Ctrl 54":"N/F","Mouse CSF Ctrl 55":"N/F","Mouse CSF Ctrl 56":"N/F","Mouse CSF Ctrl 57":"N/F","Mouse CSF Ctrl 58":"N/F","Mouse CSF Ctrl 59":"N/F","Mouse CSF Ctrl 60":"N/F","Mouse CSF Ctrl 61":"N/F","Mouse CSF Ctrl 62":"N/F","Mouse CSF Ctrl 63":"N/F","pooled sample QC 1a":"N/F","pooled sample QC 1b":"N/F","pooled sample QC 1c":"N/F","pooled sample QC 1d":"N/F","pooled sample QC 1e":"N/F","pooled sample QC 1f":"N/F","pooled sample QC 1g":"N/F","pooled sample QC 1h":"N/F","pooled sample QC 1/3 dilution":"N/F","pooled sample QC 1/10 dilution a-2x":"N/F","pooled sample QC 1/10 dilution a":"N/F","pooled sample QC 1/10 dilution b":"N/F","blank 1":"N/F","blank 2":"N/F","blank 3":"N/F","blank 4":"N/F"},{"Metabolite":"GSH_Ellmans","Mouse CSF Ctrl 1":"6898851","Mouse CSF Ctrl 2":"5423822","Mouse CSF Ctrl 3":"6749206","Mouse CSF Ctrl 4":"6646625","Mouse CSF Ctrl 5":"7995875","Mouse CSF Ctrl 6":"6351669","Mouse CSF Ctrl 7":"7877775","Mouse CSF Ctrl 8":"4843969","Mouse CSF Ctrl 9":"6652754","Mouse CSF Ctrl 10":"7475234","Mouse CSF Ctrl 11":"5496072","Mouse CSF Ctrl 12":"4650239","Mouse CSF Ctrl 13":"5892392","Mouse CSF Ctrl 14":"5130672","Mouse CSF Ctrl 15":"5588014","Mouse CSF Ctrl 16":"6304254","Mouse CSF Ctrl 17":"6049682","Mouse CSF Ctrl 18":"4178156","Mouse CSF Ctrl 19":"6003332","Mouse CSF Ctrl 20":"8170794","Mouse CSF Ctrl 21":"6349209","Mouse CSF Ctrl 22":"6335427","Mouse CSF Ctrl 23":"4417419","Mouse CSF Ctrl 24":"5445032","Mouse CSF Ctrl 25":"5848691","Mouse CSF Ctrl 26":"5270008","Mouse CSF Ctrl 27":"6656141","Mouse CSF Ctrl 28":"7437871","Mouse CSF Ctrl 29":"4692011","Mouse CSF Ctrl 30":"6925128","Mouse CSF Ctrl 31":"6411101","Mouse CSF Ctrl 32":"5492854","Mouse CSF Ctrl 33":"6448555","Mouse CSF Ctrl 34":"6777372","Mouse CSF Ctrl 35":"6654649","Mouse CSF Ctrl 36":"4223337","Mouse CSF Ctrl 37":"5112654","Mouse CSF Ctrl 38":"N/F","Mouse CSF Ctrl 39":"3924535","Mouse CSF Ctrl 40":"4656071","Mouse CSF Ctrl 41":"6149663","Mouse CSF Ctrl 42":"4020700","Mouse CSF Ctrl 43":"4297600","Mouse CSF Ctrl 44":"3513616","Mouse CSF Ctrl 45":"4496361","Mouse CSF Ctrl 46":"6938294","Mouse CSF Ctrl 47":"5198853","Mouse CSF Ctrl 48":"5371741","Mouse CSF Ctrl 49":"5562049","Mouse CSF Ctrl 50":"5223523","Mouse CSF Ctrl 51":"5614047","Mouse CSF Ctrl 52":"4976717","Mouse CSF Ctrl 53":"3425087","Mouse CSF Ctrl 54":"5788723","Mouse CSF Ctrl 55":"5652503","Mouse CSF Ctrl 56":"6534721","Mouse CSF Ctrl 57":"4975471","Mouse CSF Ctrl 58":"7446529","Mouse CSF Ctrl 59":"4708694","Mouse CSF Ctrl 60":"4737949","Mouse CSF Ctrl 61":"6801780","Mouse CSF Ctrl 62":"2459185","Mouse CSF Ctrl 63":"5118587","pooled sample QC 1a":"4629087","pooled sample QC 1b":"5697865","pooled sample QC 1c":"5813477","pooled sample QC 1d":"5471953","pooled sample QC 1e":"5462121","pooled sample QC 1f":"5652985","pooled sample QC 1g":"5876195","pooled sample QC 1h":"6557403","pooled sample QC 1/3 dilution":"847453","pooled sample QC 1/10 dilution a-2x":"104028","pooled sample QC 1/10 dilution a":"38093","pooled sample QC 1/10 dilution b":"32482","blank 1":"N/F","blank 2":"N/F","blank 3":"N/F","blank 4":"N/F"},{"Metabolite":"GSSG","Mouse CSF Ctrl 1":"1386203","Mouse CSF Ctrl 2":"512592","Mouse CSF Ctrl 3":"280972","Mouse CSF Ctrl 4":"547984","Mouse CSF Ctrl 5":"961886","Mouse CSF Ctrl 6":"616787","Mouse CSF Ctrl 7":"394794","Mouse CSF Ctrl 8":"259915","Mouse CSF Ctrl 9":"798700","Mouse CSF Ctrl 10":"1052816","Mouse CSF Ctrl 11":"255075","Mouse CSF Ctrl 12":"586997","Mouse CSF Ctrl 13":"642472","Mouse CSF Ctrl 14":"1133217","Mouse CSF Ctrl 15":"405983","Mouse CSF Ctrl 16":"892042","Mouse CSF Ctrl 17":"722967","Mouse CSF Ctrl 18":"915403","Mouse CSF Ctrl 19":"504196","Mouse CSF Ctrl 20":"649253","Mouse CSF Ctrl 21":"744868","Mouse CSF Ctrl 22":"73044","Mouse CSF Ctrl 23":"122437","Mouse CSF Ctrl 24":"253189","Mouse CSF Ctrl 25":"94898","Mouse CSF Ctrl 26":"1734161","Mouse CSF Ctrl 27":"2887013","Mouse CSF Ctrl 28":"3757859","Mouse CSF Ctrl 29":"345003","Mouse CSF Ctrl 30":"654393","Mouse CSF Ctrl 31":"451246","Mouse CSF Ctrl 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QC 1c":"1308708","pooled sample QC 1d":"1015880","pooled sample QC 1e":"942436","pooled sample QC 1f":"820260","pooled sample QC 1g":"728871","pooled sample QC 1h":"768433","pooled sample QC 1/3 dilution":"183788","pooled sample QC 1/10 dilution a-2x":"N/F","pooled sample QC 1/10 dilution a":"N/F","pooled sample QC 1/10 dilution b":"20212","blank 1":"N/F","blank 2":"N/F","blank 3":"N/F","blank 4":"N/F"}],

"Metabolites":[{"metabolite_name":"GSH-13C2_15N"},{"metabolite_name":"GSH_13C2-15N-Ellmans"},{"metabolite_name":"GSH"},{"metabolite_name":"GSH_Ellmans"},{"metabolite_name":"GSSG"}]
}

}