{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002133","ANALYSIS_ID":"AN003488","VERSION":"1","CREATED_ON":"March 31, 2022, 3:40 pm"},

"PROJECT":{"PROJECT_TITLE":"Untarget metabolomics of HIV patients carrier","PROJECT_TYPE":"NMR-based metabolomics","PROJECT_SUMMARY":"Analysis of metabolite variation in HIV patients carrier blood serum in response to treatment with effervencent glutamine.","INSTITUTE":"University of Campinas","DEPARTMENT":"Organic Chemistry","LABORATORY":"Biological Chemistry Lab","LAST_NAME":"Martins","FIRST_NAME":"Lucas","ADDRESS":"Cidade Universitário Zeferino Vaz, Campinas, SP, Brazil","EMAIL":"lgmartins1984@gmail.com","PHONE":"+5519992947044","FUNDING_SOURCE":"FAPESP, INCT-Bio"},

"STUDY":{"STUDY_TITLE":"Metabolite variation in the blood serum of HIV patients carrier treated with effervecent glutamine","STUDY_TYPE":"Liquid NMR experiments","STUDY_SUMMARY":"It was demonstrated that effervescent glutamine supplementation in people living with HIV/AIDS increased the amount of CD4+ T lymphocytes. , decreased the serum levels of biomarkers of inflammation, and introduced health benefits. Herein, NMR spectroscopy was applied to evaluate if oral ingestion of glutamine (12.5 g) for 30 days can be measured through serum metabolite variations and if the reported benefits might correlate to small metabolites' changes in a chosen cohort of individuals. The HIV/AIDS group has been carefully selected and studied before and after the 30-days of supplementation with the glutamine, together with the matched non-HIV carrier patients' group. The group of HIV/AIDS individuals presented lower (p < 0.05) levels of choline, creatine, pyruvate, glutamate, lysine, and tyrosine when compared to the non-HIV carrier patients. On the other hand, serum concentrations of glucose, lipids, lactate, glutamine, phenylalanine, and threonine were higher (p < 0.05) in HIV/AIDS individuals. The variation in metabolites as a result of treatment with glutamine is consistent with the improvements observed in these patients and may lead us to suggest the introduction of the effervescent glutamine supplementation to antiretroviral therapies in people living with HIV/AIDS.","INSTITUTE":"University of Campinas","DEPARTMENT":"Organic Chemistry","LABORATORY":"Biological Chemistry Lab","LAST_NAME":"MARTINS","FIRST_NAME":"LUCAS","ADDRESS":"Rua Josué de Castro, s/n - Cidade Universitária, Campinas - SP, 13083-970","EMAIL":"lgmartins1984@gmail.com","PHONE":"+5519992947044","NUM_GROUPS":"3","TOTAL_SUBJECTS":"24","NUM_MALES":"10","NUM_FEMALES":"14","STUDY_COMMENTS":"Crossover study"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"40.3 ± 3.9","HUMAN_NUTRITION":"Effervecent glutamine administration (12.5g, once a day for 30 days)","HUMAN_INCLUSION_CRITERIA":"Patients living with HIV from Public Service Health Care for Sexually Transmitted Diseases/AIDS Center of Maringá City, Paraná, Brazil, with same ART treatment for at least 1 year.","HUMAN_EXCLUSION_CRITERIA":"Patients which were transferred, who abandoned treatment, and those who had not been evaluated for viral load and CD4+ T lymphocyte counts were excluded."},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"1pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"1pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"2pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"2pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"4pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"4pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"6pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"6pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"8pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"8pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"9pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"9pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"10pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"10pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"12pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"12pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"13pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"13pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"16pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"16pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"14pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"14pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"17pre1",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"17pre1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"2pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"2pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"4pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"4pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"6pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"6pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"8pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"8pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"9pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"9pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"10pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"10pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"12pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"12pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"13pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"13pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"14pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"14pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"15pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"15pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"16pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"16pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"17pre2",
"Factors":{"Sample class":"BT"},
"Additional sample data":{"RAW_FILE_NAME":"17pre2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"1pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"1pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"2pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"2pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"4pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"4pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"6pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"6pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"8pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"8pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"9pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"9pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"10pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"10pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"12pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"12pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"13pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"13pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"14pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"14pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"15pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"15pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"17pos1",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"17pos1","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"1pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"1pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"4pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"4pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"6pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"6pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"8pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"8pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"10pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"10pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"12pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"12pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"13pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"13pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"14pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"14pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"15pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"15pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"16pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"16pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
},
{
"Subject ID":"-",
"Sample ID":"17pos2",
"Factors":{"Sample class":"AT"},
"Additional sample data":{"RAW_FILE_NAME":"17pos2","NMR sample ID":"3","Sample description":"Blood serum","NMR experiment":"1H CPMG"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Serum samples were obtained from 12 HIV/AIDS patients before (day 0) and after glutamine (day 31) administration, and from healthy donors.","SAMPLE_TYPE":"Blood (serum)","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"Glutamine supplementation (12.5g/day for 30 days)"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Serum samples were carefully separated and aliquoted in duplicates (250 μL each one) into cryovials and stored at -80 °C. Samples (250 μL) were thawed on ice, mixed with 250 μL of deuterium oxide (D2O 99.9%, Cambridge Isotope Laboratories, Inc., Andover, USA) at room temperature, centrifuged at 12,000 × g for 2 min at 4 °C, and transferred into 5 mm NMR tubes","PROCESSING_STORAGE_CONDITIONS":"-80℃"},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR"},

"NM":{"INSTRUMENT_NAME":"Avance 600 Bruker","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","SPECTROMETER_FREQUENCY":"600 MHz for 1H","NMR_PROBE":"inverse triple-core probe (TBI)","NMR_SOLVENT":"H2O/D2O","NMR_TUBE_SIZE":"5 mm","NMR_RESULTS_FILE":"ST002133_AN003488_Results.txt UNITS:intensity"}

}