{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002249","ANALYSIS_ID":"AN003675","VERSION":"1","CREATED_ON":"08-24-2022"}, "PROJECT":{"PROJECT_TITLE":"Sex-dependent effects of FGF21 on hepatic metabolism","PROJECT_SUMMARY":"Untargeted metabolomics analysis of livers from male and female mice administered FGF21 or saline vehicle.","INSTITUTE":"University of California, Davis","LAST_NAME":"Chaffin","FIRST_NAME":"Aki","ADDRESS":"1 Shields Ave","EMAIL":"atchaffin@ucdavis.edu","PHONE":"603-359-1907","PUBLICATIONS":"https://doi.org/10.1172/jci.insight.155848","DOI":"http://dx.doi.org/10.21228/M8ZX31"}, "STUDY":{"STUDY_TITLE":"Sex-dependent effects of FGF21 on hepatic metabolism","STUDY_SUMMARY":"To investigate sex-dependent effects of fibroblast growth factor-21 (FGF21), diet-induced obese male and female wildtype mice were administered two acute doses of FGF21 or saline vehicle. Mice were euthanized, livers collected, and submitted for untargeted metabolomics analysis.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Neurobiology, Physiology and Behavior","LABORATORY":"Ryan","LAST_NAME":"Chaffin","FIRST_NAME":"Aki","ADDRESS":"1 Shields Ave","EMAIL":"atchaffin@ucdavis.edu","PHONE":"603-359-1907","SUBMIT_DATE":"2022-08-09"}, "SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","GENDER":"Male and female","ANIMAL_LIGHT_CYCLE":"12:12","ANIMAL_FEED":"60% high-fat diet (Research Diets Cat# D12492)"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"23", "Sample ID":"23_016", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa23_1.cdf"} }, { "Subject ID":"27", "Sample ID":"27_004", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa30_1.cdf"} }, { "Subject ID":"28", "Sample ID":"28_024", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa44_1.cdf"} }, { "Subject ID":"30", "Sample ID":"30_020", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180824bJWsa01_1.cdf"} }, { "Subject ID":"34", "Sample ID":"34_028", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa47_1.cdf"} }, { "Subject ID":"37", "Sample ID":"37_036", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa37_1.cdf"} }, { "Subject ID":"38", "Sample ID":"38_012", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa49_1.cdf"} }, { "Subject ID":"40", "Sample ID":"40_008", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa15_1.cdf"} }, { "Subject ID":"41", "Sample ID":"41_032", "Factors":{"Sex":"Female","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa40_1.cdf"} }, { "Subject ID":"24", "Sample ID":"24_023", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa16_1.cdf"} }, { "Subject ID":"25", "Sample ID":"25_035", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa45_1.cdf"} }, { "Subject ID":"26", "Sample ID":"26_019", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa38_1.cdf"} }, { "Subject ID":"29", "Sample ID":"29_011", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180824bJWsa03_1.cdf"} }, { "Subject ID":"31", "Sample ID":"31_003", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa25_1.cdf"} }, { "Subject ID":"32", "Sample ID":"32_039", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa33_1.cdf"} }, { "Subject ID":"33", "Sample ID":"33_015", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa27_1.cdf"} }, { "Subject ID":"35", "Sample ID":"35_031", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa34_1.cdf"} }, { "Subject ID":"36", "Sample ID":"36_027", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa19_1.cdf"} }, { "Subject ID":"39", "Sample ID":"39_007", "Factors":{"Sex":"Female","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa20_1.cdf"} }, { "Subject ID":"10", "Sample ID":"10_022", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180824bJWsa02_1.cdf"} }, { "Subject ID":"1", "Sample ID":"1_034", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa24_1.cdf"} }, { "Subject ID":"13", "Sample ID":"13_041", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa43_1.cdf"} }, { "Subject ID":"14", "Sample ID":"14_038", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa18_1.cdf"} }, { "Subject ID":"17", "Sample ID":"17_010", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa26_1.cdf"} }, { "Subject ID":"18", "Sample ID":"18_018", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa17_1.cdf"} }, { "Subject ID":"21", "Sample ID":"21_002", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa35_1.cdf"} }, { "Subject ID":"22", "Sample ID":"22_026", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa21_1.cdf"} }, { "Subject ID":"3", "Sample ID":"3_030", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa28_1.cdf"} }, { "Subject ID":"7", "Sample ID":"7_006", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa46_1.cdf"} }, { "Subject ID":"8", "Sample ID":"8_014", "Factors":{"Sex":"Male","Treatment":"FGF21"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa14_1.cdf"} }, { "Subject ID":"11", "Sample ID":"11_025", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa22_1.cdf"} }, { "Subject ID":"12", "Sample ID":"12_013", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa48_1.cdf"} }, { "Subject ID":"15", "Sample ID":"15_009", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa42_1.cdf"} }, { "Subject ID":"16", "Sample ID":"16_029", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa13_1.cdf"} }, { "Subject ID":"19", "Sample ID":"19_040", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa39_1.cdf"} }, { "Subject ID":"20", "Sample ID":"20_005", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa31_1.cdf"} }, { "Subject ID":"2", "Sample ID":"2_037", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa50_1.cdf"} }, { "Subject ID":"4", "Sample ID":"4_017", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa41_1.cdf"} }, { "Subject ID":"5", "Sample ID":"5_021", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa29_1.cdf"} }, { "Subject ID":"6", "Sample ID":"6_033", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa32_1.cdf"} }, { "Subject ID":"9", "Sample ID":"9_001", "Factors":{"Sex":"Male","Treatment":"Saline"}, "Additional sample data":{"RAW_FILE_NAME":"180823bJWsa36_1.cdf"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Mice were euthanized and livers collected and frozen in liquid N2.","SAMPLE_TYPE":"Liver","STORAGE_CONDITIONS":"-80℃"}, "TREATMENT":{"TREATMENT_SUMMARY":"Male and female C57BL/6J mice were placed on 60% high-fat diet (Research Diets, Cat# D12492) for approx. 10 weeks beginning at 10 weeks of age. Mice were then counterbalanced into two treatment groups based on body weight (n = 9-11 mice/ sex/ treatment). For experiment, one dose of FGF21 (0.1 mg/kg, ip) or saline vehicle was given right before the onset of the dark cycle, then another dose (0.1 mg/kg, ip) was given the following morning after the onset of the light cycle. Mice were euthanized two hours following the AM injection, livers collected, and frozen in liquid N2.","TREATMENT_COMPOUND":"recombinant human FGF21 (ProSpecBio, Cat# CYT-474)","TREATMENT_ROUTE":"intraperitoneal injection","TREATMENT_DOSE":"0.1 mg/kg","TREATMENT_DOSEVOLUME":"0.01 mg/mL","TREATMENT_VEHICLE":"saline"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"See attached file.","SAMPLEPREP_PROTOCOL_ID":"methods_sampleprep.pdf","SAMPLEPREP_PROTOCOL_FILENAME":"methods_sampleprep.pdf"}, "CHROMATOGRAPHY":{"METHODS_FILENAME":"methods_GC.pdf","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)","CHROMATOGRAPHY_TYPE":"GC"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"methods_dataprocessing.pdf"}, "MS":{"INSTRUMENT_NAME":"Agilent 7890A","INSTRUMENT_TYPE":"GC-TOF","MS_TYPE":"EI","MS_COMMENTS":"See attached file.","ION_MODE":"POSITIVE","MS_RESULTS_FILE":"ST002249_AN003675_Results.txt UNITS:peak Has m/z:Yes"} }