{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002249","ANALYSIS_ID":"AN003675","VERSION":"1","CREATED_ON":"08-24-2022"},

"PROJECT":{"PROJECT_TITLE":"Sex-dependent effects of FGF21 on hepatic metabolism","PROJECT_SUMMARY":"Untargeted metabolomics analysis of livers from male and female mice administered FGF21 or saline vehicle.","INSTITUTE":"University of California, Davis","LAST_NAME":"Chaffin","FIRST_NAME":"Aki","ADDRESS":"1 Shields Ave","EMAIL":"atchaffin@ucdavis.edu","PHONE":"603-359-1907","PUBLICATIONS":"https://doi.org/10.1172/jci.insight.155848","DOI":"http://dx.doi.org/10.21228/M8ZX31"},

"STUDY":{"STUDY_TITLE":"Sex-dependent effects of FGF21 on hepatic metabolism","STUDY_SUMMARY":"To investigate sex-dependent effects of fibroblast growth factor-21 (FGF21), diet-induced obese male and female wildtype mice were administered two acute doses of FGF21 or saline vehicle. Mice were euthanized, livers collected, and submitted for untargeted metabolomics analysis.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Neurobiology, Physiology and Behavior","LABORATORY":"Ryan","LAST_NAME":"Chaffin","FIRST_NAME":"Aki","ADDRESS":"1 Shields Ave","EMAIL":"atchaffin@ucdavis.edu","PHONE":"603-359-1907","SUBMIT_DATE":"2022-08-09"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","GENDER":"Male and female","ANIMAL_LIGHT_CYCLE":"12:12","ANIMAL_FEED":"60% high-fat diet (Research Diets Cat# D12492)"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"23",
"Sample ID":"23_016",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa23_1.cdf"}
},
{
"Subject ID":"27",
"Sample ID":"27_004",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa30_1.cdf"}
},
{
"Subject ID":"28",
"Sample ID":"28_024",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa44_1.cdf"}
},
{
"Subject ID":"30",
"Sample ID":"30_020",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180824bJWsa01_1.cdf"}
},
{
"Subject ID":"34",
"Sample ID":"34_028",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa47_1.cdf"}
},
{
"Subject ID":"37",
"Sample ID":"37_036",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa37_1.cdf"}
},
{
"Subject ID":"38",
"Sample ID":"38_012",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa49_1.cdf"}
},
{
"Subject ID":"40",
"Sample ID":"40_008",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa15_1.cdf"}
},
{
"Subject ID":"41",
"Sample ID":"41_032",
"Factors":{"Sex":"Female","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa40_1.cdf"}
},
{
"Subject ID":"24",
"Sample ID":"24_023",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa16_1.cdf"}
},
{
"Subject ID":"25",
"Sample ID":"25_035",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa45_1.cdf"}
},
{
"Subject ID":"26",
"Sample ID":"26_019",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa38_1.cdf"}
},
{
"Subject ID":"29",
"Sample ID":"29_011",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180824bJWsa03_1.cdf"}
},
{
"Subject ID":"31",
"Sample ID":"31_003",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa25_1.cdf"}
},
{
"Subject ID":"32",
"Sample ID":"32_039",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa33_1.cdf"}
},
{
"Subject ID":"33",
"Sample ID":"33_015",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa27_1.cdf"}
},
{
"Subject ID":"35",
"Sample ID":"35_031",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa34_1.cdf"}
},
{
"Subject ID":"36",
"Sample ID":"36_027",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa19_1.cdf"}
},
{
"Subject ID":"39",
"Sample ID":"39_007",
"Factors":{"Sex":"Female","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa20_1.cdf"}
},
{
"Subject ID":"10",
"Sample ID":"10_022",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180824bJWsa02_1.cdf"}
},
{
"Subject ID":"1",
"Sample ID":"1_034",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa24_1.cdf"}
},
{
"Subject ID":"13",
"Sample ID":"13_041",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa43_1.cdf"}
},
{
"Subject ID":"14",
"Sample ID":"14_038",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa18_1.cdf"}
},
{
"Subject ID":"17",
"Sample ID":"17_010",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa26_1.cdf"}
},
{
"Subject ID":"18",
"Sample ID":"18_018",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa17_1.cdf"}
},
{
"Subject ID":"21",
"Sample ID":"21_002",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa35_1.cdf"}
},
{
"Subject ID":"22",
"Sample ID":"22_026",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa21_1.cdf"}
},
{
"Subject ID":"3",
"Sample ID":"3_030",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa28_1.cdf"}
},
{
"Subject ID":"7",
"Sample ID":"7_006",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa46_1.cdf"}
},
{
"Subject ID":"8",
"Sample ID":"8_014",
"Factors":{"Sex":"Male","Treatment":"FGF21"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa14_1.cdf"}
},
{
"Subject ID":"11",
"Sample ID":"11_025",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa22_1.cdf"}
},
{
"Subject ID":"12",
"Sample ID":"12_013",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa48_1.cdf"}
},
{
"Subject ID":"15",
"Sample ID":"15_009",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa42_1.cdf"}
},
{
"Subject ID":"16",
"Sample ID":"16_029",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa13_1.cdf"}
},
{
"Subject ID":"19",
"Sample ID":"19_040",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa39_1.cdf"}
},
{
"Subject ID":"20",
"Sample ID":"20_005",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa31_1.cdf"}
},
{
"Subject ID":"2",
"Sample ID":"2_037",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa50_1.cdf"}
},
{
"Subject ID":"4",
"Sample ID":"4_017",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa41_1.cdf"}
},
{
"Subject ID":"5",
"Sample ID":"5_021",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa29_1.cdf"}
},
{
"Subject ID":"6",
"Sample ID":"6_033",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa32_1.cdf"}
},
{
"Subject ID":"9",
"Sample ID":"9_001",
"Factors":{"Sex":"Male","Treatment":"Saline"},
"Additional sample data":{"RAW_FILE_NAME":"180823bJWsa36_1.cdf"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mice were euthanized and livers collected and frozen in liquid N2.","SAMPLE_TYPE":"Liver","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"Male and female C57BL/6J mice were placed on 60% high-fat diet (Research Diets, Cat# D12492) for approx. 10 weeks beginning at 10 weeks of age. Mice were then counterbalanced into two treatment groups based on body weight (n = 9-11 mice/ sex/ treatment). For experiment, one dose of FGF21 (0.1 mg/kg, ip) or saline vehicle was given right before the onset of the dark cycle, then another dose (0.1 mg/kg, ip) was given the following morning after the onset of the light cycle. Mice were euthanized two hours following the AM injection, livers collected, and frozen in liquid N2.","TREATMENT_COMPOUND":"recombinant human FGF21 (ProSpecBio, Cat# CYT-474)","TREATMENT_ROUTE":"intraperitoneal injection","TREATMENT_DOSE":"0.1 mg/kg","TREATMENT_DOSEVOLUME":"0.01 mg/mL","TREATMENT_VEHICLE":"saline"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"See attached file.","SAMPLEPREP_PROTOCOL_ID":"methods_sampleprep.pdf","SAMPLEPREP_PROTOCOL_FILENAME":"methods_sampleprep.pdf"},

"CHROMATOGRAPHY":{"METHODS_FILENAME":"methods_GC.pdf","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)","CHROMATOGRAPHY_TYPE":"GC"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"methods_dataprocessing.pdf"},

"MS":{"INSTRUMENT_NAME":"Agilent 7890A","INSTRUMENT_TYPE":"GC-TOF","MS_TYPE":"EI","MS_COMMENTS":"See attached file.","ION_MODE":"POSITIVE","MS_RESULTS_FILE":"ST002249_AN003675_Results.txt UNITS:peak Has m/z:Yes"}

}