{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002280","ANALYSIS_ID":"AN003724","VERSION":"1","CREATED_ON":"September 7, 2022, 4:59 pm"},

"PROJECT":{"PROJECT_TITLE":"Oxidative phosphorylation selectively orchestrates tissue macrophage homeostasis","PROJECT_SUMMARY":"In vitro studies associated oxidative phosphorylation (OXPHOS) with anti-inflammatory macrophages, while pro-inflammatory macrophages rely on glycolysis. However, the metabolic needs of macrophages in tissues (TMFs) to fulfil their homeostatic activities are incompletely understood. Here, we identified OXPHOS as highly discriminating process among TMFs from different tissues in homeostasis by analysis of RNAseq data, in both human and mouse. Impairing OXPHOS in TMFs via Tfam deletion differentially affected TMF populations. Tfam deletion resulted in reduction of alveolar macrophages (AMs) due to impaired lipid-handling capacity, leading to increased cholesterol content and cellular stress, causing cell cycle arrest in vivo. In obesity, Tfam depletion selectively ablated pro-inflammatory lipid-handling white adipose tissue macrophages (WAT-MFs), preventing insulin resistance and hepatosteatosis. Thus, OXPHOS, rather than glycolysis, distinguishes TMF populations and is critical for the maintenance of TMFs with a high lipid-handling activity, including pro-inflammatory WAT-MFs. This could provide a selective therapeutic targeting tool.","INSTITUTE":"Spanish National Center for Cardiovascular Research (CNIC)","DEPARTMENT":"Novel mechanisms of atherosclerosis","LABORATORY":"Immunobiology","LAST_NAME":"Mastrangelo","FIRST_NAME":"Annalaura","ADDRESS":"Calle de Melchor Fernández Almagro, 3, Centro Nacional de Investigaciones Cardiovasculares","EMAIL":"annalaura.mastrangelo@cnic.es","PHONE":"(+34) 914531200"},

"STUDY":{"STUDY_TITLE":"Oxidative phosphorylation selectively orchestrates tissue macrophage homeostasis","STUDY_TYPE":"Observational study","STUDY_SUMMARY":"In vitro studies associated oxidative phosphorylation (OXPHOS) with anti-inflammatory macrophages, while pro-inflammatory macrophages rely on glycolysis. However, the metabolic needs of macrophages in tissues (TMFs) to fulfil their homeostatic activities are incompletely understood. Here, we identified OXPHOS as highly discriminating process among TMFs from different tissues in homeostasis by analysis of RNAseq data, in both human and mouse. Impairing OXPHOS in TMFs via Tfam deletion differentially affected TMF populations. Tfam deletion resulted in reduction of alveolar macrophages (AMs) due to impaired lipid-handling capacity, leading to increased cholesterol content and cellular stress, causing cell cycle arrest in vivo. In obesity, Tfam depletion selectively ablated pro-inflammatory lipid-handling white adipose tissue macrophages (WAT-MFs), preventing insulin resistance and hepatosteatosis. Thus, OXPHOS, rather than glycolysis, distinguishes TMF populations and is critical for the maintenance of TMFs with a high lipid-handling activity, including pro-inflammatory WAT-MFs. This could provide a selective therapeutic targeting tool.","INSTITUTE":"Spanish National Center for Cardiovascular Research (CNIC)","DEPARTMENT":"Novel mechanisms of atherosclerosis","LABORATORY":"Immunobiology","LAST_NAME":"Mastrangelo","FIRST_NAME":"Annalaura","ADDRESS":"Calle de Melchor Fernández Almagro, 3, Centro Nacional de Investigaciones Cardiovasculares","EMAIL":"annalaura.mastrangelo@cnic.es","PHONE":"(+34) 914531200","NUM_GROUPS":"2"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"KO1",
"Sample ID":"KO1",
"Factors":{"Genoptype":"Mutant"},
"Additional sample data":{"RAW_FILE_NAME":"KO1.mzML"}
},
{
"Subject ID":"KO1",
"Sample ID":"KO1R",
"Factors":{"Genoptype":"Mutant"},
"Additional sample data":{"RAW_FILE_NAME":"KO1R.mzML"}
},
{
"Subject ID":"KO3",
"Sample ID":"KO3",
"Factors":{"Genoptype":"Mutant"},
"Additional sample data":{"RAW_FILE_NAME":"KO3.mzML"}
},
{
"Subject ID":"KO3",
"Sample ID":"KO3R",
"Factors":{"Genoptype":"Mutant"},
"Additional sample data":{"RAW_FILE_NAME":"KO3R.mzML"}
},
{
"Subject ID":"KO2",
"Sample ID":"KO2R",
"Factors":{"Genoptype":"Mutant"},
"Additional sample data":{"RAW_FILE_NAME":"KO2R.mzML"}
},
{
"Subject ID":"WT1",
"Sample ID":"WT1",
"Factors":{"Genoptype":"Wild_type"},
"Additional sample data":{"RAW_FILE_NAME":"WT1.mzML"}
},
{
"Subject ID":"WT1",
"Sample ID":"WT1R",
"Factors":{"Genoptype":"Wild_type"},
"Additional sample data":{"RAW_FILE_NAME":"WT1R.mzML"}
},
{
"Subject ID":"WT2",
"Sample ID":"WT2",
"Factors":{"Genoptype":"Wild_type"},
"Additional sample data":{"RAW_FILE_NAME":"WT2.mzML"}
},
{
"Subject ID":"WT2",
"Sample ID":"WT2R",
"Factors":{"Genoptype":"Wild_type"},
"Additional sample data":{"RAW_FILE_NAME":"WT2R.mzML"}
},
{
"Subject ID":"WT3",
"Sample ID":"WT3",
"Factors":{"Genoptype":"Wild_type"},
"Additional sample data":{"RAW_FILE_NAME":"WT3.mzML"}
},
{
"Subject ID":"WT3",
"Sample ID":"WT3R",
"Factors":{"Genoptype":"Wild_type"},
"Additional sample data":{"RAW_FILE_NAME":"WT3R.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mouse colonies were bred at the CNIC under specific pathogen-free conditions and on C57BL/6 background. Tfamf/f (Larsson et al., 1998) mice were kindly provided by Nils-Göran Larsson (Max Planck Institute for Biology of Ageing, Cologne, Germany). All floxed mouse lines were crossed with CD11cCre mice (Caton et al., 2007). Mice were group-housed, have not been used in previous procedures and were fed standard chow. Littermates of the same sex were randomly assigned to experimental groups. Male and female mice were used for all experiments. Mice with 6–10-weeks (adult) were used for the experiment. Bronchoalveolar lavage (BAL) was performed by inserting a venal catheter (BD) into the trachea and 3-10 washes with 0.3-1 ml FACS buffer to harvest BAL cells.","SAMPLE_TYPE":"Bronchoalveolar lavage","COLLECTION_METHOD":"Bronchoalveolar lavage (BAL) was performed by inserting a venal catheter (BD) into the trachea and 3-10 washes with 0.3-1 ml FACS buffer to harvest BAL cells.","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"Mice were group-housed and were fed standard chow."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"One million CD45+ F4/80+ CD11c+ FACS-sorted alveolar macrophages (AMs) from the bronchoalveolar lavage (BAL) of adult Tfamf/f and CD11c∆Tfam mice were collected. Each sample was generated by merging FACS-sorted AMs from 13 to 30 mice. Quality Control (QC) samples (n=4) were prepared by pooling equal volumes of cell extracts from each sample by following the same protocols used for the subject samples. Samples were subjected to two freeze–thaw cycles for metabolism quenching and complete metabolite extraction, specifically by placing the samples at -80ºC for 15 min and thawing them on ice for 10 min with brief vortex-mixing. The samples were then centrifuged at 20,000 xg at 4°C for 10 min and the supernatant collected. The supernatant was evaporated to dryness (SpeedVac Concentrator, Thermo Fisher Scientific, Waltham, MA, USA) and derivatized with 10 μl O-methoxyamine hydrochloride (15mg/mL) in pyridine and 10 μl N,O-bis(trimethylsilyl)trifluoroacetamide in 1% trimethylchlorosilane. Finally, 100 μl of heptane containing 10 ppm of 4-nitrobenzoic acid (IS) was used as internal standard to monitor sample injection"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Derivative samples (2 μL) were injected into a GC column DB5–MS (30 m length, 0.250 mm i.d., 0.25 μm film 95% dimethyl/5% diphenylpolysiloxane) with a pre–column (10 m J&W integrated with Agilent 122–5532G). The temperature gradient was programmed at 60 °C (held for 1 min), with a ramping increase rate of 10 °C/min up to 325°C (held for 10 min). The total analysis time was 37.5 min. Two analytical replicates for each sample were injected.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890B","COLUMN_NAME":"Agilent DB5-MS (30m x 0.25mm, 0.25um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 7250 GC/Q-TOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"The EI source was operated at 70 eV whereas the mass spectrometer operated in the scan mode over a mass range of m/z 50–600. Metabolite deconvolution and identification were carried out using Agilent MassHunter Unknowns Analysis version B.07.00, then, data was aligned in Agilent Mass Profiler Professional version B.12.1 and exported to Agilent MassHunter Quantitative Analysis version B.07.00. Metabolites were identified by comparing their retention time, retention index and mass fragmentation patterns with those available in an in-house library including both the NIST mass spectral database (version 2017) and Fiehn RTL library (version 2008). The different derivatives that were generated from the silylated compounds were unified by summing the abundance of all derivatives from the same metabolite. Finally, the median relative area of the two analytical replicates of the same sample was computed and used for subsequent statistical analysis."},

"MS_METABOLITE_DATA":{
"Units":"relative abundance",

"Data":[{"Metabolite":"Monoglycerol(16:0)","KO1":"1544.39","KO1R":"1859.33","KO2R":"1456","KO3":"1157.3","KO3R":"1261.46","WT1":"1419.52","WT1R":"1293.99","WT2":"901.51","WT2R":"918.9","WT3":"1196.17","WT3R":"1196.71"},{"Metabolite":"6-Deoxyglucose","KO1":"262.13","KO1R":"389.71","KO2R":"311","KO3":"282.84","KO3R":"308.35","WT1":"168","WT1R":"123.79","WT2":"230.96","WT2R":"281.02","WT3":"149.91","WT3R":"147.28"},{"Metabolite":"Alanine","KO1":"1813.68","KO1R":"2039.41","KO2R":"2171","KO3":"2773.04","KO3R":"2059.82","WT1":"1519.24","WT1R":"1514.69","WT2":"1253.32","WT2R":"1482.19","WT3":"1732.83","WT3R":"1532.32"},{"Metabolite":"Arachidic acid","KO1":"168.3","KO1R":"187.14","KO2R":"190","KO3":"194.68","KO3R":"208.16","WT1":"199.6","WT1R":"176.88","WT2":"194.92","WT2R":"183.48","WT3":"210.21","WT3R":"134.39"},{"Metabolite":"Arachidonic acid","KO1":"1279.98","KO1R":"1250.58","KO2R":"1190","KO3":"1159.05","KO3R":"1072.16","WT1":"939.55","WT1R":"893.21","WT2":"928.93","WT2R":"854.97","WT3":"1135.87","WT3R":"1011.01"},{"Metabolite":"Aspartic acid","KO1":"1685.63","KO1R":"1970.46","KO2R":"2105","KO3":"2442.26","KO3R":"2322.53","WT1":"1227.66","WT1R":"1318.78","WT2":"877.62","WT2R":"949.75","WT3":"998.36","WT3R":"930.16"},{"Metabolite":"Cholesterol","KO1":"204100.3355","KO1R":"204950.121","KO2R":"186654","KO3":"167792.5424","KO3R":"169773.9365","WT1":"110034.1367","WT1R":"108534.1513","WT2":"112531.2553","WT2R":"109774.6827","WT3":"107986.8689","WT3R":"105313.9675"},{"Metabolite":"Citric acid","KO1":"1152.92","KO1R":"1191.77","KO2R":"1151","KO3":"1091","KO3R":"1169.47","WT1":"869.22","WT1R":"967.57","WT2":"1064.91","WT2R":"1226.35","WT3":"719.11","WT3R":"703.96"},{"Metabolite":"Decanoic acid","KO1":"4376.64","KO1R":"4255.23","KO2R":"4586","KO3":"5029.79","KO3R":"4682.35","WT1":"5017.78","WT1R":"5084.47","WT2":"4651.73","WT2R":"4548","WT3":"4969.81","WT3R":"4669.5"},{"Metabolite":"Eicosapentaenoic acid","KO1":"1452.72","KO1R":"1482.11","KO2R":"5667","KO3":"9989.79","KO3R":"9745.03","WT1":"1709.84","WT1R":"1655.43","WT2":"1431","WT2R":"1495.2","WT3":"2945.34","WT3R":"3065.06"},{"Metabolite":"Fumaric acid","KO1":"760.59","KO1R":"711.51","KO2R":"738","KO3":"717.96","KO3R":"761.8","WT1":"488.34","WT1R":"518.81","WT2":"470.4","WT2R":"407.49","WT3":"496.5","WT3R":"550.58"},{"Metabolite":"Glucose","KO1":"367.55","KO1R":"460.57","KO2R":"458","KO3":"500.4","KO3R":"503.55","WT1":"301.07","WT1R":"343.37","WT2":"270.68","WT2R":"335.82","WT3":"306.02","WT3R":"218.57"},{"Metabolite":"Glutamic acid","KO1":"1433.24","KO1R":"1619.86","KO2R":"1630","KO3":"1786.23","KO3R":"1682.52","WT1":"879.97","WT1R":"869.7","WT2":"707.16","WT2R":"959.58","WT3":"886.91","WT3R":"710.34"},{"Metabolite":"Glycerol","KO1":"3017.88","KO1R":"2722.1","KO2R":"2664","KO3":"2373.91","KO3R":"2542.64","WT1":"1797.08","WT1R":"1690.93","WT2":"2108.47","WT2R":"2361.38","WT3":"2798.67","WT3R":"2627.65"},{"Metabolite":"Glycerol 1-phosphate","KO1":"865.59","KO1R":"782.07","KO2R":"758","KO3":"669.29","KO3R":"714.44","WT1":"539.55","WT1R":"486.83","WT2":"411.74","WT2R":"374.98","WT3":"622.46","WT3R":"424.1"},{"Metabolite":"Glycine","KO1":"1827.29","KO1R":"1825.22","KO2R":"2814","KO3":"2289.4","KO3R":"2046.15","WT1":"1335.91","WT1R":"1154.21","WT2":"1733.62","WT2R":"1819.12","WT3":"1549.25","WT3R":"1229.02"},{"Metabolite":"Isocitric acid","KO1":"385.45","KO1R":"478.36","KO2R":"450","KO3":"428.27","KO3R":"507","WT1":"303.91","WT1R":"280.2","WT2":"175.98","WT2R":"166.46","WT3":"223.6","WT3R":"183.62"},{"Metabolite":"Isoleucine","KO1":"1016.56","KO1R":"1052.71","KO2R":"1047","KO3":"1065.94","KO3R":"1051.58","WT1":"594.07","WT1R":"648.19","WT2":"516.06","WT2R":"504.9","WT3":"534.12","WT3R":"622.55"},{"Metabolite":"Lactic acid","KO1":"1582.21","KO1R":"1839.04","KO2R":"1754","KO3":"1829.51","KO3R":"1763.42","WT1":"1525.4","WT1R":"1490.7","WT2":"1574.46","WT2R":"1609.21","WT3":"1666.47","WT3R":"1886.25"},{"Metabolite":"Leucine","KO1":"69981.01","KO1R":"71142.5","KO2R":"58864","KO3":"47440.08","KO3R":"46891.91","WT1":"66295.54","WT1R":"67064.46","WT2":"33895.56","WT2R":"34665.72","WT3":"49295.33","WT3R":"49228.22"},{"Metabolite":"Malic acid","KO1":"229.9","KO1R":"227.42","KO2R":"221","KO3":"227.61","KO3R":"200.96","WT1":"149.51","WT1R":"141.7","WT2":"67.85","WT2R":"107.65","WT3":"143.43","WT3R":"132.3"},{"Metabolite":"Myoinositol","KO1":"1019.712435","KO1R":"904.0846988","KO2R":"948","KO3":"932.1662191","KO3R":"937.8526844","WT1":"619.5179771","WT1R":"728.9580921","WT2":"428.1399165","WT2R":"532.0223491","WT3":"369.8407219","WT3R":"333.3971281"},{"Metabolite":"Myoinositol 6phosphate","KO1":"756.15","KO1R":"740.03","KO2R":"707","KO3":"674.53","KO3R":"656.42","WT1":"513.95","WT1R":"497.54","WT2":"506.3","WT2R":"406.52","WT3":"499.46","WT3R":"401.91"},{"Metabolite":"Myristic acid","KO1":"1116.254952","KO1R":"1160.903555","KO2R":"1106","KO3":"1094.351775","KO3R":"1053.158901","WT1":"1196.207033","WT1R":"1031.864329","WT2":"1148.726676","WT2R":"1050.623068","WT3":"1185.885469","WT3R":"1129.445179"},{"Metabolite":"Oleic Acid","KO1":"1689.06","KO1R":"1620.88","KO2R":"1471","KO3":"1312.49","KO3R":"1263.04","WT1":"1427.58","WT1R":"1382.91","WT2":"1199.33","WT2R":"1253.75","WT3":"1265.91","WT3R":"1185.33"},{"Metabolite":"Palmitelaidic acid","KO1":"643.5690365","KO1R":"654.7631298","KO2R":"616","KO3":"533.0411176","KO3R":"630.6573462","WT1":"479.494271","WT1R":"440.2855699","WT2":"530.3337626","WT2R":"577.268463","WT3":"608.5112678","WT3R":"475.5485659"},{"Metabolite":"Palmitic acid","KO1":"69113.56","KO1R":"72139.78","KO2R":"65577","KO3":"59322.17","KO3R":"61733.45","WT1":"70269.62","WT1R":"70379.96","WT2":"67594.52","WT2R":"67941.95","WT3":"67367.65","WT3R":"65340.71"},{"Metabolite":"Palmitoleic acid","KO1":"434.01","KO1R":"476.15","KO2R":"366","KO3":"245.25","KO3R":"309.93","WT1":"355.59","WT1R":"349.77","WT2":"261.21","WT2R":"259.02","WT3":"344.48","WT3R":"279.03"},{"Metabolite":"Proline","KO1":"2159.32","KO1R":"2418.54","KO2R":"2498","KO3":"2852.16","KO3R":"2560.78","WT1":"1640.46","WT1R":"1706.29","WT2":"1514.53","WT2R":"1687.94","WT3":"1747.2","WT3R":"1756.99"},{"Metabolite":"Pyruvic acid","KO1":"7430.34","KO1R":"7848.19","KO2R":"7794","KO3":"7891.14","KO3R":"8007.23","WT1":"8449.62","WT1R":"8458.5","WT2":"7996.7","WT2R":"8932.16","WT3":"7861.19","WT3R":"7687"},{"Metabolite":"Serine","KO1":"1095.2","KO1R":"1262.48","KO2R":"1338","KO3":"1514.17","KO3R":"1482.14","WT1":"1100.43","WT1R":"1176.12","WT2":"962.86","WT2R":"1005.65","WT3":"1014.98","WT3R":"893.4"},{"Metabolite":"Stearic acid","KO1":"8595.44","KO1R":"8510.01","KO2R":"9314","KO3":"9825.12","KO3R":"10325.74","WT1":"10825.13","WT1R":"10845.77","WT2":"9895.13","WT2R":"9988.18","WT3":"9647.56","WT3R":"9925.36"},{"Metabolite":"Succinic acid","KO1":"1269.52","KO1R":"1397.58","KO2R":"1322","KO3":"1098.14","KO3R":"1522.73","WT1":"1233.5","WT1R":"1446.88","WT2":"1171.77","WT2R":"1051.59","WT3":"1097.97","WT3R":"1529.97"},{"Metabolite":"Threonine","KO1":"433.44","KO1R":"527.75","KO2R":"503","KO3":"557.67","KO3R":"492.75","WT1":"321.77","WT1R":"274.6","WT2":"258.1","WT2R":"212.95","WT3":"325.18","WT3R":"276.95"},{"Metabolite":"Valine","KO1":"2055.47","KO1R":"2146.87","KO2R":"2102","KO3":"2107.39","KO3R":"2096.82","WT1":"1242.2","WT1R":"1024.03","WT2":"841.44","WT2R":"1013.68","WT3":"1200.12","WT3R":"1209.19"},{"Metabolite":"Cysteine","KO1":"822.33","KO1R":"811.32","KO2R":"889","KO3":"1130.78","KO3R":"954.7","WT1":"596.6","WT1R":"388.15","WT2":"336.55","WT2R":"444.71","WT3":"557.93","WT3R":"525.47"}],

"Metabolites":[{"Metabolite":"Monoglycerol(16:0)","Pubchem ID":"14900","KEGG ID":"-","Retention_time":"23.43","Target_Ion":"371","Retention_Index":"2581"},{"Metabolite":"6-Deoxyglucose","Pubchem ID":"93579","KEGG ID":"C00586","Retention_time":"15.54","Target_Ion":"117.07","Retention_Index":"1998"},{"Metabolite":"Alanine","Pubchem ID":"5950","KEGG ID":"C00041","Retention_time":"7.404","Target_Ion":"116","Retention_Index":"777.72"},{"Metabolite":"Arachidic acid","Pubchem ID":"10467","KEGG ID":"C06425","Retention_time":"22.33","Target_Ion":"369.30","Retention_Index":"2385"},{"Metabolite":"Arachidonic acid","Pubchem ID":"444899","KEGG ID":"C00219","Retention_time":"21.681","Target_Ion":"91","Retention_Index":"2417"},{"Metabolite":"Aspartic acid","Pubchem ID":"5960","KEGG ID":"C00049","Retention_time":"12.101","Target_Ion":"160","Retention_Index":"1102.84"},{"Metabolite":"Cholesterol","Pubchem ID":"5997","KEGG ID":"C00187","Retention_time":"27.488","Target_Ion":"329","Retention_Index":"2826.78"},{"Metabolite":"Citric acid","Pubchem ID":"311","KEGG ID":"C00158","Retention_time":"16.458","Target_Ion":"273","Retention_Index":"1493.93"},{"Metabolite":"Decanoic acid","Pubchem ID":"2969","KEGG ID":"C01571","Retention_time":"16.63","Target_Ion":"229.20","Retention_Index":"1391"},{"Metabolite":"Eicosapentaenoic acid","Pubchem ID":"446284","KEGG ID":"C06428","Retention_time":"23.86","Target_Ion":"361.20","Retention_Index":"2425"},{"Metabolite":"Fumaric acid","Pubchem ID":"444972","KEGG ID":"C00122","Retention_time":"10.868","Target_Ion":"245","Retention_Index":"1025.3"},{"Metabolite":"Glucose","Pubchem ID":"5793","KEGG ID":"C00031","Retention_time":"17.36","Target_Ion":"319.20","Retention_Index":"1579.97"},{"Metabolite":"Glutamic acid","Pubchem ID":"33032","KEGG ID":"C00025","Retention_time":"14.256","Target_Ion":"246","Retention_Index":"1297.52"},{"Metabolite":"Glycerol","Pubchem ID":"753","KEGG ID":"C00116","Retention_time":"9.806","Target_Ion":"205","Retention_Index":"949.79"},{"Metabolite":"Glycerol 1-phosphate","Pubchem ID":"3707990","KEGG ID":"C00623","Retention_time":"15.879","Target_Ion":"357","Retention_Index":"1439.25"},{"Metabolite":"Glycine","Pubchem ID":"750","KEGG ID":"C00037","Retention_time":"7.66","Target_Ion":"102","Retention_Index":"1002"},{"Metabolite":"Isocitric acid","Pubchem ID":"1198","KEGG ID":"C00311","Retention_time":"16.897","Target_Ion":"273","Retention_Index":"1497.48"},{"Metabolite":"Isoleucine","Pubchem ID":"6306","KEGG ID":"C00407","Retention_time":"10.084","Target_Ion":"158","Retention_Index":"969.56"},{"Metabolite":"Lactic acid","Pubchem ID":"612","KEGG ID":"C00186","Retention_time":"6.794","Target_Ion":"147","Retention_Index":"732.96"},{"Metabolite":"Leucine","Pubchem ID":"6106","KEGG ID":"C00123","Retention_time":"7.96","Target_Ion":"86","Retention_Index":"831.85"},{"Metabolite":"Malic acid","Pubchem ID":"525","KEGG ID":"C00711","Retention_time":"12.433","Target_Ion":"233","Retention_Index":"1164.45"},{"Metabolite":"Myoinositol","Pubchem ID":"892","KEGG ID":"C00137","Retention_time":"19.29","Target_Ion":"305.10","Retention_Index":"2194"},{"Metabolite":"Myoinositol 6phosphate","Pubchem ID":"161368","KEGG ID":"C01284                      ","Retention_time":"22.12","Target_Ion":"318.20","Retention_Index":"2386"},{"Metabolite":"Myristic acid","Pubchem ID":"11005","KEGG ID":"C06424","Retention_time":"16.821","Target_Ion":"117","Retention_Index":"1524.35"},{"Metabolite":"Oleic Acid","Pubchem ID":"445639","KEGG ID":"C00712","Retention_time":"20.381","Target_Ion":"339","Retention_Index":"1892.45"},{"Metabolite":"Palmitelaidic acid","Pubchem ID":"5282745","KEGG ID":"-","Retention_time":"18.65","Target_Ion":"311.20","Retention_Index":"1995"},{"Metabolite":"Palmitic acid","Pubchem ID":"985","KEGG ID":"C00249                      ","Retention_time":"18.793","Target_Ion":"117","Retention_Index":"1720.26"},{"Metabolite":"Palmitoleic acid","Pubchem ID":" 445638","KEGG ID":"C08362","Retention_time":"18.554","Target_Ion":"311","Retention_Index":"1701.83"},{"Metabolite":"Proline","Pubchem ID":"145742","KEGG ID":"C00148","Retention_time":"10.162","Target_Ion":"142","Retention_Index":"975.16"},{"Metabolite":"Pyruvic acid","Pubchem ID":"1060","KEGG ID":"C00022","Retention_time":"6.643","Target_Ion":"174","Retention_Index":"721.67"},{"Metabolite":"Serine","Pubchem ID":"5951","KEGG ID":"C00065","Retention_time":"10.991","Target_Ion":"204","Retention_Index":"1040.14"},{"Metabolite":"Stearic acid","Pubchem ID":"5281","KEGG ID":"C01530","Retention_time":"20.608","Target_Ion":"341","Retention_Index":"1916.02"},{"Metabolite":"Succinic acid","Pubchem ID":"1110","KEGG ID":"C00042","Retention_time":"10.387","Target_Ion":"247","Retention_Index":"989.72"},{"Metabolite":"Threonine","Pubchem ID":"6288","KEGG ID":"C00188","Retention_time":"11.329","Target_Ion":"218","Retention_Index":"1063.93"},{"Metabolite":"Valine","Pubchem ID":"6287","KEGG ID":"C00183","Retention_time":"7.219","Target_Ion":"72","Retention_Index":"762.88"},{"Metabolite":"Cysteine","Pubchem ID":"5862","KEGG ID":"C00097","Retention_time":"13.43","Target_Ion":"218","Retention_Index":"1230.5"}]
}

}